JP2012533323A - ヒト胚性幹細胞の分化 - Google Patents
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Abstract
Description
本発明は、米国特許公開番号第61/226,929号(2009年7月20日出願)に対する優先権を請求する。
本発明は、PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない、膵内分泌系に特徴的なマーカーを発現している細胞への多能性幹細胞の分化を促進する方法を提供する。
a.多能性幹細胞を培養する工程、
b.多能性幹細胞を、胚体内胚葉系に特徴的なマーカーを発現している細胞へと分化させる工程、及び
c.胚体内胚葉系に特徴的なマーカーを発現している細胞を、FGF7を添加した第1培地で処理し、次いで細胞を、FGF7、BMP阻害能を持つ因子、アクチビンA、レチノイン酸、及びhedgehogシグナル経路阻害剤を添加した第2培地で培養することにより、PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない、膵臓内胚葉系に特徴的なマーカーを発現している細胞へと分化させる工程。
幹細胞は、単一の細胞レベルにて自己複製し、分化して後代細胞を生成する、それら両方の能力で定義される未分化細胞であり、後代細胞としては、自己複製前駆細胞、非再生前駆細胞、及び最終分化細胞が挙げられる。幹細胞はまた、in vitroで複数の胚葉(内胚葉、中胚葉及び外胚葉)から様々な細胞系の機能的細胞へと分化する能力によって、また移植後に複数の胚葉の組織を生じ、胚盤胞への注入後、全部ではないとしてもほとんどの組織を提供する能力によっても、特徴付けられる。
多能性幹細胞の特徴付け
多能性幹細胞は、ステージ特異的胚抗原(SSEA)3及び4、並びにTra−1−60及びTra−1−81と呼ばれる抗体によって検出可能なマーカーのうちの1つ以上を発現している(Thomsonら、Science 282:1145,1998)。in vitroで多能性幹細胞を分化させると、SSEA−4、Tra 1−60、及びTra 1−81の発現が減少し(存在する場合)、SSEA−1の発現が上昇する。未分化の多能性幹細胞は通常アルカリホスファターゼ活性を有し、これは、細胞を4%パラホルムアルデヒドで固定した後、製造業者(Vector Laboratories(Burlingame Calif.))によって記載されるようにVectorRedを基質として現像することによって検出することができる。未分化の多能性幹細胞はまた、RT−PCRで検出されるように、典型的にOct−4及びTERTも発現している。
使用が可能な多能性幹細胞の種類としては、妊娠期間中の任意の時期(必ずしもではないが、通常は妊娠約10〜12週よりも前)に採取した前胚性組織(例えば胚盤胞など)、胚性組織、胎児組織などの、妊娠後に形成される組織に由来する多能性細胞の株化細胞系が挙げられる。非限定的な例は、例えばヒト胚幹細胞株H1、H7、及びH9(WiCell)などのヒト胚幹細胞又はヒト胚生殖細胞の確立株である。それらの細胞の最初の樹立又は安定化中に本開示の組成物を使用することも想定され、その場合、供給源となる細胞は、供給源となる組織から直接採取した一次多能性細胞であろう。フィーダー細胞の不在下で既に培養された多能性幹細胞集団から採取した細胞も好適である。例えば、BG01v(BresaGen、Athens、GA)などの変異ヒト胚性幹細胞株も好適である。
一実施形態では、多能性幹細胞は、典型的にはフィーダー細胞の層上で培養され、このフィーダー細胞は、多能性幹細胞を様々な方法で支持する。あるいは、多能性幹細胞を、フィーダー細胞を本質的に含まないにも関わらず、細胞を実質的に分化させることなく多能性幹細胞の増殖を支持するような培養システム中で培養する。フィーダー細胞不含培養における多能性幹細胞の、分化を伴わない増殖は、あらかじめ他の細胞種を培養することにより馴化培地を使用して支持される。あるいはフィーダー細胞不含培養における多能性幹細胞の分化を伴わない増殖は、合成培地を使用して支持される。
一実施形態では、本発明は、多能性幹細胞から、膵臓内胚葉系に特徴的なマーカーを発現している細胞を産生するための方法を提供し、この方法は以下の工程a、b及びcを包含する:
a.多能性幹細胞を培養する工程、
b.多能性幹細胞を、胚体内胚葉系に特徴的なマーカーを発現している細胞へと分化させる工程、及び
c.胚体内胚葉系に特徴的なマーカーを発現している細胞を、膵臓内胚葉系に特徴的なマーカーを発現している細胞へと分化させる工程。
胚体内胚葉系に特徴的なマーカーを発現している細胞の形成は、以下の特定のプロトコルの前後に、マーカーの存在に関して試験することにより決定することができる。多能性幹細胞は、一般にこのようなマーカーを発現しない。したがって、多能性細胞の分化は、細胞がそれらの発現を開始した際に検出される。
一実施形態では、胚体内胚葉系に特徴的なマーカーを発現している細胞を、FGF7を添加した第1培地で培養し、次いで細胞を、FGF7、BMP阻害能を持つ因子、アクチビンA、レチノイン酸、及びhedgehogシグナル経路阻害剤を添加した第2培地で培養することにより、PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない、膵臓内胚葉系に特徴的なマーカーを発現している細胞へと分化させる。
一実施形態では、本発明の方法により産生される、PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない、膵臓内胚葉系に特徴的なマーカーを発現している細胞は、膵内分泌系に特徴的なマーカーを発現している細胞へと更に分化し得る。
一態様では、本発明は、I型糖尿病に罹患しているかあるいはI型糖尿病を発症するリスクを有する患者を治療する方法を提供する。一実施形態では、本方法は、多能性幹細胞を培養することと、多能性幹細胞をin vitroでβ細胞系に分化させることと、β細胞系の細胞を患者に移植することと、を包含する。別の実施形態では、本方法は、多能性幹細胞を培養することと、多能性幹細胞を、PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない、膵臓内胚葉系に特徴的なマーカーを発現している細胞へとin vitroで分化させることと、PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない膵臓内胚葉系細胞を患者に移植することと、を包含する。
PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない、膵臓内胚葉系に特徴的なマーカーを発現している細胞へのヒト多能性幹細胞の分化
本実施例は、アクチビンAをノギン及びレチノイン酸と組み合わせて使用することで、NKX6.1発現の上方制御を促進できることを示す。簡潔に述べると、ヒト胚性幹細胞株H1細胞を、MATRIGEL(商標)(1:30希釈)をコートしたディッシュと、2%のBSA、100ng/mLのアクチビンA、20ng/mLのWNT−3a、8ng/mLのbFGFを添加したRPMI培地により1日培養した後で、2%のBSA、100ng/mLのアクチビンA、8ng/mLのbFGFを添加したRPMI培地で更に2日間処理し(ステージ1)、次いで
a.2%のBSA、50ng/mLのFGF7及び0.25μMのシクロパミン−KAADを添加したDMEM/F12で3日間にわたって培養し(ステージ2)、次いで
b.1%のB27、50ng/mLのFGF7、0.25μMのシクロパミン−KAAD、2μMのレチノイン酸(RA)、100ng/mLのノギン、及び20ng/mLのアクチビンA若しくは50ng/mLのアクチビンAを添加したDMEM(高グルコース)で4日間にわたって培養した(ステージ3)。
a.2%のBSA(カタログ# 152401,MP Biomedical,Ohio)、100ng/mLのアクチビンA(R&D Systems,MN)、20ng/mLのWNT−3a(カタログ# 1324−WN−002,R&D Systems,MN)及び8ng/mLのbFGF(カタログ# 100−18B,PeproTech,NJ)を添加したRPMI培地(カタログ#22400,Invitrogen,Ca)で1日培養した後に、2%のBSA、100ng/mLのアクチビンA、8ng/mLのbFGFを添加したRPMI培地で更に2日間にわたって処理し(ステージ1)、次いで
b.2%のBSA及び50ng/mLのFGF7を添加したDMEM/F12(カタログ#11330,Invitrogen,Ca)で3日間にわたって培養し(ステージ2)、次いで
c.処理1:1%のB27(Invitrogen,CA)、50ng/mLのFGF7、0.25μMのシクロパミン−KAAD、2μMのレチノイン酸(RA)及び100ng/mLのノギンを添加したDMEM(高グルコース)で4日間にわたって処理するか、又は
d.処理2:1%のB27(Invitrogen,CA)、50ng/mLのFGF7、0.25μMのシクロパミン−KAAD、2μMのレチノイン酸(RA)、100ng/mLのノギン、20ng/mLのアクチビンAを添加したDMEM(高グルコース)で4日間にわたって処理するか、又は
e.処理3:1%のB27(Invitrogen,CA)、50ng/mLのFGF7、0.25μMのシクロパミン−KAAD、2μMのレチノイン酸(RA)、100ng/mLのノギン及び1μMのALK5阻害剤IIを添加したDMEM(高グルコース)で4日間にわたって処理した。
Claims (1)
- PDX−1及びNKX−6.1は共発現するが、CDX−2及びNGN−3は発現しない、膵臓内胚葉系に特徴的なマーカーを発現している細胞集団へと、多能性幹細胞集団を分化させる方法であって、以下の工程a、b及びcを包含する方法:
a.前記多能性幹細胞を培養する工程、
b.前記多能性幹細胞を、胚体内胚葉系に特徴的なマーカーを発現している細胞へと分化させる工程、及び
c.前記胚体内胚葉系に特徴的なマーカーを発現している細胞を、FGF7を添加した第1培地で処理し、次いで前記細胞を、FGF7、BMP阻害能を持つ因子、アクチビンA、レチノイン酸、及びhedgehogシグナル経路阻害剤を添加した第2培地で培養することにより、PDX1及びNKX6.1は共発現するが、CDX2及びNGN3は発現しない、膵臓内胚葉系に特徴的なマーカーを発現している細胞へと分化させる工程。
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PCT/US2010/042504 WO2011011349A2 (en) | 2009-07-20 | 2010-07-20 | Differentiation of human embryonic stem cells |
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WO2018139600A1 (ja) * | 2017-01-27 | 2018-08-02 | 株式会社カネカ | 内胚葉系細胞集団、及び多能性細胞から三胚葉のいずれかの細胞集団を製造する方法 |
Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
CN101484575B (zh) | 2005-06-08 | 2013-10-02 | 森托科尔公司 | 用于眼变性的细胞疗法 |
US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
US8685730B2 (en) | 2006-05-02 | 2014-04-01 | Wisconsin Alumni Research Foundation | Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage |
US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
CN101952415B (zh) | 2007-07-31 | 2017-06-27 | 生命扫描有限公司 | 人胚胎干细胞的分化 |
CN107574142B (zh) | 2007-11-27 | 2021-07-06 | 生命扫描有限公司 | 人胚胎干细胞的分化 |
WO2009105570A2 (en) | 2008-02-21 | 2009-08-27 | Centocor Ortho Biotech Inc. | Methods, surface modified plates and compositions for cell attachment, cultivation and detachment |
US8623648B2 (en) | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
JP5734183B2 (ja) | 2008-06-30 | 2015-06-17 | ヤンセン バイオテツク,インコーポレーテツド | 多能性幹細胞の分化 |
CA2742267C (en) | 2008-10-31 | 2019-06-04 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells to the pancreatic endocrine lineage |
BRPI0919885A2 (pt) | 2008-10-31 | 2015-08-11 | Centocor Ortho Biotech Inc | Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática |
AU2009316583B2 (en) | 2008-11-20 | 2016-04-21 | Janssen Biotech, Inc. | Methods and compositions for cell attachment and cultivation on planar substrates |
RU2555538C2 (ru) | 2008-11-20 | 2015-07-10 | Сентокор Орто Байотек Инк. | Культура плюрипотентных стволовых клеток на микроносителях |
GB2485113B (en) | 2009-07-20 | 2016-12-28 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into cells of the pancreatic endoderm lineage |
RU2540021C2 (ru) | 2009-07-20 | 2015-01-27 | Янссен Байотек, Инк. | Дифференцировка эмбриональных стволовых клеток человека |
EP2456862A4 (en) | 2009-07-20 | 2013-02-27 | Janssen Biotech Inc | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS |
RU2586506C2 (ru) | 2009-12-23 | 2016-06-10 | Янссен Байотек, Инк. | Дифференцировка человеческих эмбриональных стволовых клеток |
CN102741395B (zh) | 2009-12-23 | 2016-03-16 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
AU2011223900A1 (en) | 2010-03-01 | 2012-09-13 | Janssen Biotech, Inc. | Methods for purifying cells derived from pluripotent stem cells |
MX351515B (es) | 2010-05-12 | 2017-10-17 | Janssen Biotech Inc | Diferenciacion de celulas madre embrionarias humanas. |
CA2809305C (en) | 2010-08-31 | 2019-06-11 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
AU2011296381B2 (en) | 2010-08-31 | 2016-03-31 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
US9528090B2 (en) | 2010-08-31 | 2016-12-27 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
ITMI20110780A1 (it) * | 2011-05-06 | 2012-11-07 | Euroclone Spa | Terreno di coltura per differenziare cellule staminali in cellule beta |
WO2012170853A1 (en) * | 2011-06-10 | 2012-12-13 | Wisconsin Alumni Research Foundation ("Warf") | Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage |
AU2012355698B2 (en) | 2011-12-22 | 2018-11-29 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
KR20140131999A (ko) | 2012-03-07 | 2014-11-14 | 얀센 바이오테크 인코포레이티드 | 만능 줄기 세포의 증폭 및 유지를 위한 한정 배지 |
EP2847319A4 (en) * | 2012-05-07 | 2015-12-16 | Janssen Biotech Inc | DIFFERENTIATION OF HUMAN EMBRYONAL STEM CELLS IN THE PANCREAS ENDODERM |
CN108103006A (zh) | 2012-06-08 | 2018-06-01 | 詹森生物科技公司 | 人胚胎干细胞向胰腺内分泌细胞的分化 |
US20150247123A1 (en) | 2012-09-03 | 2015-09-03 | Novo Nordisk A/S | Generation of pancreatic endoderm from Pluripotent Stem cells using small molecules |
US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
JP6529440B2 (ja) | 2012-12-31 | 2019-06-12 | ヤンセン バイオテツク,インコーポレーテツド | 膵内分泌細胞への分化のためのヒト多能性細胞の懸濁及びクラスタリング |
KR102084561B1 (ko) | 2012-12-31 | 2020-03-04 | 얀센 바이오테크 인코포레이티드 | 췌장 내분비 세포로의 분화를 위한 공기-액체 계면에서의 인간 배아 줄기세포의 배양 |
KR102036780B1 (ko) | 2012-12-31 | 2019-10-25 | 얀센 바이오테크 인코포레이티드 | Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화 |
CN103194424A (zh) * | 2013-03-28 | 2013-07-10 | 于涛 | 一种诱导胚胎干细胞为胰腺组织样细胞的方法 |
RU2016100219A (ru) | 2013-06-11 | 2017-07-17 | Президент Энд Феллоус Оф Гарвард Колледж | КЛЕТКИ SC-β И КОМПОЗИЦИИ, И СПОСОБЫ ДЛЯ ИХ СОЗДАНИЯ |
CA2949056A1 (en) | 2014-05-16 | 2015-11-19 | Janssen Biotech, Inc. | Use of small molecules to enhance mafa expression in pancreatic endocrine cells |
WO2016044721A1 (en) | 2014-09-19 | 2016-03-24 | Regenerative Medical Solutions, Inc. | Compositions and methods for differentiating stem cells into cell populations comprising beta-like cells |
EP3234110B1 (en) | 2014-12-18 | 2024-02-28 | President and Fellows of Harvard College | METHODS FOR GENERATING STEM CELL-DERIVED ß CELLS AND USES THEREOF |
US10253298B2 (en) | 2014-12-18 | 2019-04-09 | President And Fellows Of Harvard College | Methods for generating stem cell-derived beta cells and methods of use thereof |
US10443042B2 (en) | 2014-12-18 | 2019-10-15 | President And Fellows Of Harvard College | Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof |
MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
IL305391A (en) | 2017-11-15 | 2023-10-01 | Vertex Pharma | Preparations for the production of islet cells and methods of use |
AU2019320072A1 (en) | 2018-08-10 | 2021-02-25 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
US20200080107A1 (en) | 2018-09-07 | 2020-03-12 | Crispr Therapeutics Ag | Universal donor cells |
JP2022533208A (ja) * | 2019-05-22 | 2022-07-21 | ザ クリーブランド クリニック ファウンデーション | 背側前腸及び前方ドメイン内胚葉細胞の生成 |
EP3975926A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates, Inc. | A biocompatible membrane composite |
CN114401752B (zh) | 2019-05-31 | 2023-04-04 | W.L.戈尔及同仁股份有限公司 | 具有受控氧扩散距离的细胞封装装置 |
CA3139591C (en) | 2019-05-31 | 2024-01-16 | Timothy M. BRUHN | A biocompatible membrane composite |
CN114173837A (zh) | 2019-05-31 | 2022-03-11 | W.L.戈尔及同仁股份有限公司 | 生物相容性膜复合材料 |
CA3150233A1 (en) | 2019-09-05 | 2021-03-11 | Alireza Rezania | UNIVERSAL DONOR CELLS |
JP2022547505A (ja) | 2019-09-05 | 2022-11-14 | クリスパー セラピューティクス アクチェンゲゼルシャフト | ユニバーサルドナー細胞 |
WO2022144855A1 (en) | 2020-12-31 | 2022-07-07 | Crispr Therapeutics Ag | Universal donor cells |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003050249A2 (en) * | 2001-12-07 | 2003-06-19 | Geron Corporation | Islet cells from human embryonic stem cells |
WO2005086860A2 (en) * | 2004-03-09 | 2005-09-22 | Gang Xu | Methods for generating insulin-producing cells |
WO2007051038A2 (en) * | 2005-10-27 | 2007-05-03 | Cythera, Inc. | Pdx1-expressing dorsal and ventral foregut endoderm |
Family Cites Families (174)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3209652A (en) | 1961-03-30 | 1965-10-05 | Burgsmueller Karl | Thread whirling method |
AT326803B (de) | 1968-08-26 | 1975-12-29 | Binder Fa G | Maschenware sowie verfahren zur herstellung derselben |
US3935067A (en) | 1974-11-22 | 1976-01-27 | Wyo-Ben Products, Inc. | Inorganic support for culture media |
CA1201400A (en) | 1982-04-16 | 1986-03-04 | Joel L. Williams | Chemically specific surfaces for influencing cell activity during culture |
US4499802A (en) | 1982-09-29 | 1985-02-19 | Container Graphics Corporation | Rotary cutting die with scrap ejection |
US4537773A (en) | 1983-12-05 | 1985-08-27 | E. I. Du Pont De Nemours And Company | α-Aminoboronic acid derivatives |
US4557264A (en) | 1984-04-09 | 1985-12-10 | Ethicon Inc. | Surgical filament from polypropylene blended with polyethylene |
US5089396A (en) | 1985-10-03 | 1992-02-18 | Genentech, Inc. | Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid |
US5215893A (en) | 1985-10-03 | 1993-06-01 | Genentech, Inc. | Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid |
US4737578A (en) | 1986-02-10 | 1988-04-12 | The Salk Institute For Biological Studies | Human inhibin |
US5863531A (en) * | 1986-04-18 | 1999-01-26 | Advanced Tissue Sciences, Inc. | In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework |
CA1340581C (en) | 1986-11-20 | 1999-06-08 | Joseph P. Vacanti | Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices |
US5804178A (en) * | 1986-11-20 | 1998-09-08 | Massachusetts Institute Of Technology | Implantation of cell-matrix structure adjacent mesentery, omentum or peritoneum tissue |
US5567612A (en) | 1986-11-20 | 1996-10-22 | Massachusetts Institute Of Technology | Genitourinary cell-matrix structure for implantation into a human and a method of making |
EP0363125A3 (en) | 1988-10-03 | 1990-08-16 | Hana Biologics Inc. | Proliferated pancreatic endocrine cell product and process |
US5837539A (en) * | 1990-11-16 | 1998-11-17 | Osiris Therapeutics, Inc. | Monoclonal antibodies for human mesenchymal stem cells |
US5449383A (en) * | 1992-03-18 | 1995-09-12 | Chatelier; Ronald C. | Cell growth substrates |
GB9206861D0 (en) * | 1992-03-28 | 1992-05-13 | Univ Manchester | Wound healing and treatment of fibrotic disorders |
CA2114282A1 (en) | 1993-01-28 | 1994-07-29 | Lothar Schilder | Multi-layered implant |
JP3525221B2 (ja) | 1993-02-17 | 2004-05-10 | 味の素株式会社 | 免疫抑制剤 |
US5523226A (en) | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
GB9310557D0 (en) * | 1993-05-21 | 1993-07-07 | Smithkline Beecham Plc | Novel process and apparatus |
TW257671B (ja) | 1993-11-19 | 1995-09-21 | Ciba Geigy | |
US5834308A (en) * | 1994-04-28 | 1998-11-10 | University Of Florida Research Foundation, Inc. | In vitro growth of functional islets of Langerhans |
US6703017B1 (en) | 1994-04-28 | 2004-03-09 | Ixion Biotechnology, Inc. | Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures |
US6001647A (en) | 1994-04-28 | 1999-12-14 | Ixion Biotechnology, Inc. | In vitro growth of functional islets of Langerhans and in vivo uses thereof |
US6083903A (en) | 1994-10-28 | 2000-07-04 | Leukosite, Inc. | Boronic ester and acid compounds, synthesis and uses |
DE69525971T3 (de) | 1994-12-29 | 2013-01-10 | Chugai Seiyaku K.K. | Verwendung eines pm-1 antikörpers oder eines mh 166 antikörpers zur verstärkung des anti-tumor-effektes von cisplatin oder carboplatin |
US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
US5718922A (en) * | 1995-05-31 | 1998-02-17 | Schepens Eye Research Institute, Inc. | Intravitreal microsphere drug delivery and method of preparation |
US5908782A (en) * | 1995-06-05 | 1999-06-01 | Osiris Therapeutics, Inc. | Chemically defined medium for human mesenchymal stem cells |
UA65572C2 (en) | 1997-04-24 | 2004-04-15 | Ortho Mcneil Pharm Inc | Substituted imidazoles, intermediate compounds for the preparation thereof, a method for the preparation of substituted imidazoles and a method for the treatment of inflammatory diseases |
DE69837491T2 (de) * | 1997-07-03 | 2008-01-17 | Osiris Therapeutics, Inc. | Menschliche mesenchymale stammzellen aus peripherem blut |
ATE462004T1 (de) | 1997-09-16 | 2010-04-15 | Centocor Inc | Methoden zur kompletten chemischen synthese und zusammensetzung von genen und genomen |
US6670127B2 (en) | 1997-09-16 | 2003-12-30 | Egea Biosciences, Inc. | Method for assembly of a polynucleotide encoding a target polypeptide |
AU729377B2 (en) * | 1997-10-23 | 2001-02-01 | Asterias Biotherapeutics, Inc. | Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture |
ZA9811898B (en) * | 1997-12-29 | 2000-06-28 | Ortho Mcneil Pharm Inc | Anti-Inflammatory Compounds. |
AU755888B2 (en) | 1998-03-18 | 2003-01-02 | Mesoblast International Sarl | Mesenchymal stem cells for prevention and treatment of immune responses in transplantation |
MY132496A (en) | 1998-05-11 | 2007-10-31 | Vertex Pharma | Inhibitors of p38 |
US6667176B1 (en) * | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
US7410798B2 (en) | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
US6413556B1 (en) * | 1999-01-08 | 2002-07-02 | Sky High, Llc | Aqueous anti-apoptotic compositions |
US6458593B1 (en) * | 1999-01-21 | 2002-10-01 | Vitro Diagnostics, Inc. | Immortalized cell lines and methods of making the same |
US6815203B1 (en) | 1999-06-23 | 2004-11-09 | Joslin Diabetes Center, Inc. | Methods of making pancreatic islet cells |
US6333029B1 (en) | 1999-06-30 | 2001-12-25 | Ethicon, Inc. | Porous tissue scaffoldings for the repair of regeneration of tissue |
US6306424B1 (en) | 1999-06-30 | 2001-10-23 | Ethicon, Inc. | Foam composite for the repair or regeneration of tissue |
US6685936B2 (en) | 1999-10-12 | 2004-02-03 | Osiris Therapeutics, Inc. | Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation |
US20030082155A1 (en) | 1999-12-06 | 2003-05-01 | Habener Joel F. | Stem cells of the islets of langerhans and their use in treating diabetes mellitus |
EP1240518A4 (en) * | 1999-12-13 | 2006-05-17 | Scripps Research Inst | MARKERS FOR THE IDENTIFICATION AND INSULATION OF PRE-GENERIC CELLS OF A AND B PANCREAS ISOLATED CELLS |
US7439064B2 (en) | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
US7005252B1 (en) | 2000-03-09 | 2006-02-28 | Wisconsin Alumni Research Foundation | Serum free cultivation of primate embryonic stem cells |
US6436704B1 (en) | 2000-04-10 | 2002-08-20 | Raven Biotechnologies, Inc. | Human pancreatic epithelial progenitor cells and methods of isolation and use thereof |
US6458589B1 (en) * | 2000-04-27 | 2002-10-01 | Geron Corporation | Hepatocyte lineage cells derived from pluripotent stem cells |
EP1302534A4 (en) * | 2000-06-26 | 2004-06-16 | Renomedix Inst Inc | CELL FRACTIONS CONTAINING CELLS CAPABLE OF DIFFERENCING INTO NEURAL CELLS |
CZ20031125A3 (cs) * | 2000-10-23 | 2003-10-15 | Smithkline Beecham Corporation | Nové sloučeniny |
ATE301661T1 (de) | 2000-12-08 | 2005-08-15 | Ortho Mcneil Pharm Inc | Makroheterocyclische verbindungen als kinase inhibitoren |
ATE326464T1 (de) | 2000-12-08 | 2006-06-15 | Ortho Mcneil Pharm Inc | Indazolyl-substituierte pyrrolidin-verbindungen als kinase inhibitoren |
US6599323B2 (en) * | 2000-12-21 | 2003-07-29 | Ethicon, Inc. | Reinforced tissue implants and methods of manufacture and use |
EP1366148A2 (en) | 2001-01-24 | 2003-12-03 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE DEPARTMENT OF HEALTH & HUMAN SERVICES | Differentiation of stem cells to pancreatic endocrine cells |
AU2002243646B2 (en) | 2001-01-25 | 2006-06-22 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Formulation of boronic acid compounds |
US6656488B2 (en) * | 2001-04-11 | 2003-12-02 | Ethicon Endo-Surgery, Inc. | Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering |
JP2004527249A (ja) * | 2001-04-19 | 2004-09-09 | デヴェロゲン アクチエンゲゼルシャフト フュア エントヴィックルングスビオローギッシェ フォルシュング | 幹細胞をインスリン産生細胞に分化する方法 |
JP4296781B2 (ja) | 2001-04-24 | 2009-07-15 | 味の素株式会社 | 幹細胞及びその分離方法 |
WO2002092756A2 (en) | 2001-05-15 | 2002-11-21 | Rappaport Family Institute For Research In The Medical Sciences | Insulin producing cells derived from human embryonic stem cells |
US6626950B2 (en) * | 2001-06-28 | 2003-09-30 | Ethicon, Inc. | Composite scaffold with post anchor for the repair and regeneration of tissue |
KR100418195B1 (ko) | 2001-07-05 | 2004-02-11 | 주식회사 우리기술 | 전력케이블의 다중절연진단장치 및 그 방법 |
GB0117583D0 (en) * | 2001-07-19 | 2001-09-12 | Astrazeneca Ab | Novel compounds |
CA2456981C (en) * | 2001-08-06 | 2012-02-28 | Bresagen, Inc. | Alternative compositions and methods for the culture of stem cells |
US6617152B2 (en) * | 2001-09-04 | 2003-09-09 | Corning Inc | Method for creating a cell growth surface on a polymeric substrate |
WO2003033697A1 (en) | 2001-10-18 | 2003-04-24 | Ixion Biotechnology, Inc. | Conversion of liver stem and progenitor cells to pancreatic functional cells |
EP1442115B9 (en) | 2001-11-15 | 2009-12-16 | Children's Medical Center Corporation | Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof |
EP1572071B1 (en) | 2001-12-07 | 2018-10-03 | Cytori Therapeutics, Inc. | Methods for preparing fresh adipose tissue-derived cells and uses in treating patients |
AU2002218893A1 (en) | 2001-12-21 | 2003-07-09 | Thromb-X Nv | Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability |
US20030162290A1 (en) | 2002-01-25 | 2003-08-28 | Kazutomo Inoue | Method for inducing differentiation of embryonic stem cells into functioning cells |
US20050208029A1 (en) * | 2002-04-17 | 2005-09-22 | Akihiro Umezawa | Method of forming pancreatic beta cells from mesenchymal cells |
US20040161419A1 (en) * | 2002-04-19 | 2004-08-19 | Strom Stephen C. | Placental stem cells and uses thereof |
ATE387444T1 (de) | 2002-05-08 | 2008-03-15 | Janssen Pharmaceutica Nv | Substituierte pyrroline als kinase inhibitoren |
US20060003446A1 (en) * | 2002-05-17 | 2006-01-05 | Gordon Keller | Mesoderm and definitive endoderm cell populations |
JP2005527241A (ja) | 2002-05-28 | 2005-09-15 | ベクトン・ディキンソン・アンド・カンパニー | invitroにおけるヒト膵臓腺房細胞の増殖およびインスリン産生細胞への分化転換のための方法 |
WO2003104222A1 (en) | 2002-06-05 | 2003-12-18 | Janssen Pharmaceutica N.V. | Bisindolyl-maleimid derivatives as kinase inhibitors |
GB0212976D0 (en) | 2002-06-06 | 2002-07-17 | Tonejet Corp Pty Ltd | Ejection method and apparatus |
CN1171991C (zh) | 2002-07-08 | 2004-10-20 | 徐如祥 | 人神经干细胞的培养方法 |
US6877147B2 (en) * | 2002-07-22 | 2005-04-05 | Broadcom Corporation | Technique to assess timing delay by use of layout quality analyzer comparison |
US7838290B2 (en) * | 2002-07-25 | 2010-11-23 | The Scripps Research Institute | Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith |
US20040110287A1 (en) | 2002-07-29 | 2004-06-10 | Es Cell International Pte Ltd. | Multi-step method for the differentiation of insulin positive, glucose responsive cells |
US20040063204A1 (en) | 2002-08-14 | 2004-04-01 | Lijun Yang | Bone marrow cell differentiation |
AU2003268534A1 (en) | 2002-09-06 | 2004-03-29 | Amcyte Inc. | Cd56 positive human adult pancreatic endocrine progenitor cells |
US9969977B2 (en) | 2002-09-20 | 2018-05-15 | Garnet Biotherapeutics | Cell populations which co-express CD49c and CD90 |
US20040062753A1 (en) * | 2002-09-27 | 2004-04-01 | Alireza Rezania | Composite scaffolds seeded with mammalian cells |
AU2003285172A1 (en) | 2002-11-08 | 2004-06-03 | The Johns Hopkins University | Human embryonic stem cell cultures, and compositions and methods for growing same |
US7144999B2 (en) * | 2002-11-23 | 2006-12-05 | Isis Pharmaceuticals, Inc. | Modulation of hypoxia-inducible factor 1 alpha expression |
AU2003302702B2 (en) | 2002-12-05 | 2008-08-07 | Technion Research & Development Foundation Ltd. | Cultured human pancreatic islets, and uses thereof |
ES2571355T3 (es) | 2002-12-16 | 2016-05-24 | Technion Res & Dev Foundation | Sistema de cultivo sin células alimentadoras ni xenocontaminantes para células madre embrionarias humanas |
US20070154981A1 (en) * | 2003-02-14 | 2007-07-05 | The Board Of Trustees Of The Leland Stanford Junior University | Insulin-producing cells derived from stem cells |
WO2004073633A2 (en) | 2003-02-14 | 2004-09-02 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for modulating the development of stem cells |
CA2520861A1 (en) | 2003-03-27 | 2004-10-14 | Ixion Biotechnology, Inc. | Method for transdifferentiation of non-pancreatic stem cells to the pancreatic pathway |
WO2004090110A2 (en) | 2003-03-31 | 2004-10-21 | Bresagen Inc. | Compositions and methods for the control, differentiation and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway |
US20090203141A1 (en) | 2003-05-15 | 2009-08-13 | Shi-Lung Lin | Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents |
WO2005001079A2 (en) | 2003-06-27 | 2005-01-06 | Ethicon, Incorporated | Soft tissue repair and regeneration using postpartum-derived cells |
IL161903A0 (en) | 2003-07-17 | 2005-11-20 | Gamida Cell Ltd | Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs |
ITRM20030395A1 (it) | 2003-08-12 | 2005-02-13 | Istituto Naz Per Le Malattie Infettive Lazz | Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero. |
US20050042595A1 (en) | 2003-08-14 | 2005-02-24 | Martin Haas | Banking of multipotent amniotic fetal stem cells |
US7157275B2 (en) * | 2003-08-15 | 2007-01-02 | Becton, Dickinson And Company | Peptides for enhanced cell attachment and growth |
WO2005021728A2 (en) | 2003-08-27 | 2005-03-10 | Stemcells California, Inc. | Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for these populations |
EP1696899A1 (en) | 2003-12-17 | 2006-09-06 | Allergan, Inc. | Methods for treating retinoid responsive disorders using selective inhibitors of cyp26a and cyp26b |
US20060030042A1 (en) | 2003-12-19 | 2006-02-09 | Ali Brivanlou | Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime |
EA200601231A1 (ru) | 2003-12-23 | 2007-06-29 | Китера, Инк. | Дефинитивная энтодерма |
US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
US20050266554A1 (en) * | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
US20050233446A1 (en) * | 2003-12-31 | 2005-10-20 | Parsons Xuejun H | Defined media for stem cell culture |
TWI334443B (en) * | 2003-12-31 | 2010-12-11 | Ind Tech Res Inst | Method of single cell culture of undifferentiated human embryonic stem cells |
US20080241107A1 (en) | 2004-01-23 | 2008-10-02 | Copland Iii John A | Methods and Compositions For Preparing Pancreatic Insulin Secreting Cells |
WO2005080551A2 (en) | 2004-02-12 | 2005-09-01 | University Of Newcastle Upon Tyne | Stem cells |
WO2005086845A2 (en) | 2004-03-10 | 2005-09-22 | Regents Of The University Of California | Compositions and methods for growth of embryonic stem cells |
EP1730268A2 (en) | 2004-04-01 | 2006-12-13 | Wisconsin Alumni Research Foundation | Differentiation of stem cells to endoderm and pancreatic lineage |
CN103103158B (zh) | 2004-04-27 | 2016-08-03 | 韦尔赛特公司 | 细胞培养基 |
CA2966883A1 (en) | 2004-07-09 | 2006-02-16 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
WO2006020919A2 (en) | 2004-08-13 | 2006-02-23 | University Of Georgia Research Foundation, Inc. | Compositions and methods for self-renewal and differentiation in human embryonic stem cells |
US20080268533A1 (en) | 2004-08-25 | 2008-10-30 | University Of Georgia Research Foundation, Inc. | Methods and Compositions Utilizing Myc and Gsk3Beta to Manipulate the Pluripotency of Embryonic Stem Cells |
DE102004043256B4 (de) | 2004-09-07 | 2013-09-19 | Rheinische Friedrich-Wilhelms-Universität Bonn | Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension |
NZ553235A (en) | 2004-09-08 | 2009-11-27 | Wisconsin Alumni Res Found | Culturing human pluripotent stem cells |
MX2007002390A (es) | 2004-09-08 | 2007-04-23 | Wisconsin Alumni Res Found | Medio y cultivo de celulas progenitoras embrionarias. |
WO2006083782A2 (en) | 2005-01-31 | 2006-08-10 | Es Cell International Pte Ltd. | Directed differentiation of embryonic stem cells and uses thereof |
CN101188942B (zh) | 2005-03-04 | 2011-11-30 | 生命扫描有限公司 | 成年胰衍生的基质细胞 |
GB0505970D0 (en) | 2005-03-23 | 2005-04-27 | Univ Edinburgh | Culture medium containing kinase inhibitor, and uses thereof |
ATE553198T1 (de) | 2005-04-15 | 2012-04-15 | Geron Corp | Behandlung von krebs durch die kombinierte hemmung der proteasom- und telomeraseaktivitäten |
EP1874367B1 (en) | 2005-04-26 | 2011-07-06 | Arhus Universitet | Biocompatible material for surgical implants and cell guiding tissue culture surfaces |
AU2006257859B2 (en) | 2005-06-10 | 2009-12-10 | Irm Llc | Compounds that maintain pluripotency of embryonic stem cells |
WO2006138433A2 (en) | 2005-06-14 | 2006-12-28 | The Regents Of The University Of California | Induction of cell differentiation by class i bhlh polypeptides |
US20080199959A1 (en) | 2005-06-21 | 2008-08-21 | Ge Healthcare Bio-Sciences Ab | Method For Cell Culture |
CN101341138B (zh) | 2005-06-30 | 2012-11-14 | 詹森药业有限公司 | 作为gsk-3抑制剂的环状苯胺基-吡啶并三嗪类 |
AU2006274438A1 (en) | 2005-07-29 | 2007-02-01 | Australian Stem Cell Centre Limited | Compositions and methods for growth of pluripotent cells |
WO2007016485A2 (en) | 2005-07-29 | 2007-02-08 | Athersys, Inc. | Use of a gsk-3 inhibitor to maintain potency of cultured cells |
BRPI0617085A2 (pt) | 2005-09-02 | 2016-11-08 | Agency Science Tech & Res | método para obter uma célula-tronco mesenquimal (msc), célula ou linhagem celular, método para derivar uma cultura celular a partir de uma célula-tronco embrionária, método para tratamento de uma doença, uso de uma célula-tronco mesenquimal, linhagem diferenciada, composição farmacêutica, método para condicionar um meio de cultura celular, meio condicionado e uso do mesmo, mpetodo para obterum polipeptídeo, método para obtar uma cultura celular, cultura celular, célula-tronco mesenquimal, linhagem de célula-tronco mesenquimal ou uma célula mesenquimal diferenciada |
WO2007030870A1 (en) | 2005-09-12 | 2007-03-22 | Es Cell International Pte Ltd | Cardiomyocyte production |
JP2009511061A (ja) | 2005-10-14 | 2009-03-19 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | 膵臓表現型を有する細胞への非胚性幹細胞の分化 |
WO2007082963A1 (es) | 2006-01-18 | 2007-07-26 | Fundación Instituto Valenciano De Infertilidad | Líneas de células madre embrionarias humanas y métodos para usar las mismas |
CN105802904B (zh) | 2006-02-23 | 2021-04-20 | 维亚赛特公司 | 用于培养可分化细胞的组合物和方法 |
AU2007224116B2 (en) | 2006-03-02 | 2013-11-07 | Viacyte, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
CA2984541C (en) | 2006-04-28 | 2022-04-12 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
GB2452186B (en) * | 2006-05-02 | 2011-01-26 | Wisconsin Alumni Res Found | Method of differentiating stem cells into cells of the endoderm and pancreatic lineage |
US7964402B2 (en) | 2006-05-25 | 2011-06-21 | Sanford-Burnham Medical Research Institute | Methods for culture and production of single cell populations of human embryonic stem cells |
WO2007149182A2 (en) | 2006-06-19 | 2007-12-27 | Geron Corporation | Differentiation and enrichment of islet-like cells from human pluripotent stem cells |
CN100494359C (zh) | 2006-06-23 | 2009-06-03 | 中日友好医院 | 神经干细胞三维立体培养体外扩增的方法 |
US20080003676A1 (en) | 2006-06-26 | 2008-01-03 | Millipore Corporation | Growth of embryonic stem cells |
CA2893679C (en) | 2006-06-26 | 2018-10-30 | Lifescan, Inc. | Conditioned medium for pluripotent stem cell culture |
WO2008004990A2 (en) | 2006-07-06 | 2008-01-10 | Es Cell International Pte Ltd | Method for stem cell culture and cells derived therefrom |
WO2008013664A2 (en) | 2006-07-26 | 2008-01-31 | Cythera, Inc. | Methods of producing pancreatic hormones |
JP2008099662A (ja) | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
WO2008039521A2 (en) * | 2006-09-26 | 2008-04-03 | Nmt Medical, Inc. | Method for modifying a medical implant surface for promoting tissue growth |
US20100323442A1 (en) | 2006-10-17 | 2010-12-23 | Emmanuel Edward Baetge | Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells |
CA2666789C (en) | 2006-10-18 | 2016-11-22 | Yong Zhao | Embryonic-like stem cells derived from adult human peripheral blood and methods of use |
TW200836749A (en) | 2007-01-09 | 2008-09-16 | Vioquest Pharmaceuticals Inc | Compositions including triciribine and bortezomib and derivatives thereof and methods of use thereof |
US9175260B2 (en) | 2007-01-30 | 2015-11-03 | TheUniversity of Georgia Research Foundation, Inc. | Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (MMC) |
GB0703188D0 (en) | 2007-02-19 | 2007-03-28 | Roger Land Building | Large scale production of stem cells |
CN105176919A (zh) | 2007-07-18 | 2015-12-23 | 生命扫描有限公司 | 人胚胎干细胞的分化 |
CN101952415B (zh) | 2007-07-31 | 2017-06-27 | 生命扫描有限公司 | 人胚胎干细胞的分化 |
CA2696622C (en) | 2007-08-24 | 2016-07-19 | Stichting Het Nederlands Kanker Instituut | Compositions for the treatment of neoplastic diseases |
CN107574142B (zh) | 2007-11-27 | 2021-07-06 | 生命扫描有限公司 | 人胚胎干细胞的分化 |
SG154367A1 (en) | 2008-01-31 | 2009-08-28 | Es Cell Int Pte Ltd | Method of differentiating stem cells |
US20100330677A1 (en) | 2008-02-11 | 2010-12-30 | Cambridge Enterprise Limited | Improved Reprogramming of Mammalian Cells, and Cells Obtained |
WO2009105570A2 (en) | 2008-02-21 | 2009-08-27 | Centocor Ortho Biotech Inc. | Methods, surface modified plates and compositions for cell attachment, cultivation and detachment |
SG188918A1 (ja) | 2008-03-17 | 2013-04-30 | Agency Science Tech & Res | |
AU2008355123B2 (en) | 2008-04-21 | 2014-12-04 | Viacyte, Inc. | Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells |
US20090298178A1 (en) | 2008-06-03 | 2009-12-03 | D Amour Kevin Allen | Growth factors for production of definitive endoderm |
JP5734183B2 (ja) | 2008-06-30 | 2015-06-17 | ヤンセン バイオテツク,インコーポレーテツド | 多能性幹細胞の分化 |
DE102008032236A1 (de) | 2008-06-30 | 2010-04-01 | Eberhard-Karls-Universität Tübingen | Isolierung und/oder Identifizierung von Stammzellen mit adipozytärem, chondrozytärem und pankreatischem Differenzierungspotential |
US20100028307A1 (en) | 2008-07-31 | 2010-02-04 | O'neil John J | Pluripotent stem cell differentiation |
BRPI0919885A2 (pt) | 2008-10-31 | 2015-08-11 | Centocor Ortho Biotech Inc | Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática |
US8008075B2 (en) | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
CA2768218C (en) | 2009-07-15 | 2018-02-20 | The University Of Akron | Manufacturing of multifunctional electrically conductive/transparent/flexible films |
GB2485113B (en) | 2009-07-20 | 2016-12-28 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into cells of the pancreatic endoderm lineage |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003050249A2 (en) * | 2001-12-07 | 2003-06-19 | Geron Corporation | Islet cells from human embryonic stem cells |
WO2005086860A2 (en) * | 2004-03-09 | 2005-09-22 | Gang Xu | Methods for generating insulin-producing cells |
WO2007051038A2 (en) * | 2005-10-27 | 2007-05-03 | Cythera, Inc. | Pdx1-expressing dorsal and ventral foregut endoderm |
Non-Patent Citations (9)
Title |
---|
JPN6014047896; Development Vol.125, 1998, p.4863-4876 * |
JPN6014047897; Development Vol.129, 2002, p.4685-4696 * |
JPN6014047898; Gene Expression Patterns Vol.6, 2006, p.783-793 * |
JPN6014047901; Development Vol.126, 1999, p.723-732 * |
JPN6014047903; J.Hepatol. Author Manuscript Vol.49, No.3, 200901, p.384-395 * |
JPN6014047905; Development Vol.129, 2002, p.1819-1828 * |
JPN6014047910; Developmental Dynamics Vol.238, Issue 12, 200910, p.3035-3042 * |
JPN6014047913; BMC Genomics Vol.9, 2008, p.196 * |
JPN6014047915; Anthony, K. et al.: 'Signaling events mediated by the Hedgehog family' Pathway Interaction Database [online] , 2012, Pathway ID: hedgehog_2pathway, National Cancer Institute * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2018139600A1 (ja) * | 2017-01-27 | 2018-08-02 | 株式会社カネカ | 内胚葉系細胞集団、及び多能性細胞から三胚葉のいずれかの細胞集団を製造する方法 |
JPWO2018139600A1 (ja) * | 2017-01-27 | 2019-11-21 | 株式会社カネカ | 内胚葉系細胞集団、及び多能性細胞から三胚葉のいずれかの細胞集団を製造する方法 |
JP7107504B2 (ja) | 2017-01-27 | 2022-07-27 | 株式会社カネカ | 内胚葉系細胞集団、及び多能性細胞から三胚葉のいずれかの細胞集団を製造する方法 |
US11746331B2 (en) | 2017-01-27 | 2023-09-05 | Kaneka Corporation | Endodermal cell population, and method for producing cell population of any of three germ layers from pluripotent cell |
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JP5819826B2 (ja) | 2015-11-24 |
US8785185B2 (en) | 2014-07-22 |
KR101786735B1 (ko) | 2017-10-18 |
ZA201201220B (en) | 2013-07-31 |
CN102597219A (zh) | 2012-07-18 |
CN102597219B (zh) | 2015-08-19 |
US20110014703A1 (en) | 2011-01-20 |
AU2010276402B2 (en) | 2014-07-03 |
RU2012105924A (ru) | 2013-08-27 |
CN103952372A (zh) | 2014-07-30 |
KR20120037986A (ko) | 2012-04-20 |
MX2012000898A (es) | 2012-06-01 |
EP2456859A2 (en) | 2012-05-30 |
RU2540016C2 (ru) | 2015-01-27 |
EP2456859A4 (en) | 2015-03-18 |
GB2485113A (en) | 2012-05-02 |
WO2011011349A3 (en) | 2011-05-05 |
CN103952372B (zh) | 2016-10-05 |
GB2485113B (en) | 2016-12-28 |
BR112012001564A2 (pt) | 2015-09-01 |
CA2768720C (en) | 2018-12-18 |
GB201202848D0 (en) | 2012-04-04 |
AR077766A1 (es) | 2011-09-21 |
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