JP2012509084A5 - - Google Patents
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- JP2012509084A5 JP2012509084A5 JP2011537596A JP2011537596A JP2012509084A5 JP 2012509084 A5 JP2012509084 A5 JP 2012509084A5 JP 2011537596 A JP2011537596 A JP 2011537596A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2012509084 A5 JP2012509084 A5 JP 2012509084A5
- Authority
- JP
- Japan
- Prior art keywords
- restriction site
- nucleic acid
- linker sequence
- orientation
- restriction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 102000039446 nucleic acids Human genes 0.000 claims 54
- 108020004707 nucleic acids Proteins 0.000 claims 54
- 150000007523 nucleic acids Chemical class 0.000 claims 54
- 102000040430 polynucleotide Human genes 0.000 claims 50
- 108091033319 polynucleotide Proteins 0.000 claims 50
- 239000002157 polynucleotide Substances 0.000 claims 50
- 239000013598 vector Substances 0.000 claims 36
- 108091008146 restriction endonucleases Proteins 0.000 claims 20
- 230000000295 complement effect Effects 0.000 claims 12
- 238000000137 annealing Methods 0.000 claims 9
- 238000004519 manufacturing process Methods 0.000 claims 6
- 230000007850 degeneration Effects 0.000 claims 4
- 230000029087 digestion Effects 0.000 claims 4
- 230000006801 homologous recombination Effects 0.000 claims 4
- 238000002744 homologous recombination Methods 0.000 claims 4
- 230000015572 biosynthetic process Effects 0.000 claims 3
- 238000003776 cleavage reaction Methods 0.000 claims 3
- 238000002844 melting Methods 0.000 claims 3
- 230000008018 melting Effects 0.000 claims 3
- 230000007017 scission Effects 0.000 claims 3
- 238000003786 synthesis reaction Methods 0.000 claims 3
- 230000001131 transforming effect Effects 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 2
- 102000003960 Ligases Human genes 0.000 claims 2
- 108090000364 Ligases Proteins 0.000 claims 2
- 238000004925 denaturation Methods 0.000 claims 2
- 230000036425 denaturation Effects 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 2
- 229930024421 Adenine Natural products 0.000 claims 1
- 108010042407 Endonucleases Proteins 0.000 claims 1
- 102000004533 Endonucleases Human genes 0.000 claims 1
- 229960000643 adenine Drugs 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- 239000005549 deoxyribonucleoside Substances 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
- 229940113082 thymine Drugs 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11610908P | 2008-11-19 | 2008-11-19 | |
| US61/116,109 | 2008-11-19 | ||
| US16223009P | 2009-03-20 | 2009-03-20 | |
| US61/162,230 | 2009-03-20 | ||
| PCT/US2009/065048 WO2010059763A1 (en) | 2008-11-19 | 2009-11-19 | Compositions and methods for the assembly of polynucleotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012509084A JP2012509084A (ja) | 2012-04-19 |
| JP2012509084A5 true JP2012509084A5 (enExample) | 2012-10-11 |
Family
ID=41698076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011537596A Pending JP2012509084A (ja) | 2008-11-19 | 2009-11-19 | ポリヌクレオチドアセンブリに関する組成物及び方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US8221982B2 (enExample) |
| EP (1) | EP2358875B1 (enExample) |
| JP (1) | JP2012509084A (enExample) |
| KR (1) | KR101794298B1 (enExample) |
| CN (1) | CN102282255B (enExample) |
| AU (1) | AU2009316660B2 (enExample) |
| BR (1) | BRPI0922187B1 (enExample) |
| CA (1) | CA2744153C (enExample) |
| MX (1) | MX2011005195A (enExample) |
| SG (1) | SG171760A1 (enExample) |
| WO (1) | WO2010059763A1 (enExample) |
| ZA (1) | ZA201103637B (enExample) |
Families Citing this family (62)
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| WO2010059763A1 (en) * | 2008-11-19 | 2010-05-27 | Amyris Biotechnologies, Inc. | Compositions and methods for the assembly of polynucleotides |
| US7919605B1 (en) | 2010-08-30 | 2011-04-05 | Amyris, Inc. | Nucleic acids, compositions and methods for the excision of target nucleic acids |
| SG194089A1 (en) | 2011-04-27 | 2013-11-29 | Amyris Inc | Methods for genomic modification |
| CN104039974B (zh) | 2011-11-09 | 2016-10-12 | 阿迈瑞斯公司 | 乙酰辅酶a衍生的类异戊二烯的生产 |
| US8332160B1 (en) | 2011-11-17 | 2012-12-11 | Amyris Biotechnologies, Inc. | Systems and methods for engineering nucleic acid constructs using scoring techniques |
| US20150141714A1 (en) * | 2012-01-13 | 2015-05-21 | Chromatin, Inc. | Engineering plants with rate limiting farnesene metabolic genes |
| US9738890B2 (en) | 2012-03-27 | 2017-08-22 | Dsm Ip Assets B.V. | Cloning method |
| ES2663424T3 (es) * | 2012-06-29 | 2018-04-12 | Massachusetts Institute Of Technology | Genética combinatoria masivamente en paralelo |
| WO2014092886A2 (en) | 2012-12-10 | 2014-06-19 | Agilent Technologies, Inc. | Pairing code directed assembly |
| EP2971027B1 (en) | 2013-03-15 | 2019-01-30 | Amyris, Inc. | Use of phosphoketolase and phosphotransacetylase for production of acetyl-coenzyme a derived compounds |
| DK2986635T3 (en) | 2013-04-18 | 2019-01-28 | Fond Telethon | EFFECTIVE DELIVERY OF BIG GENES THROUGH DUAL-AAV VECTORS |
| US9476065B2 (en) | 2013-12-19 | 2016-10-25 | Amyris, Inc. | Methods for genomic integration |
| US10287627B2 (en) | 2014-02-08 | 2019-05-14 | The Regents Of The University Of Colorado, A Body Corporate | Multiplexed linking PCR |
| WO2015184016A2 (en) * | 2014-05-27 | 2015-12-03 | The Broad Institute, Inc. | High-thoughput assembly of genetic elements |
| CN114365792A (zh) | 2014-11-11 | 2022-04-19 | 克莱拉食品公司 | 用于生成卵清蛋白的方法和组合物 |
| WO2016090266A1 (en) | 2014-12-05 | 2016-06-09 | Amyris, Inc. | High-throughput sequencing of polynucleotides |
| US20180327779A1 (en) * | 2015-03-03 | 2018-11-15 | Fondazione Telethon (IT/IT) | Multiple vector system and uses thereof |
| CN107849600A (zh) | 2015-06-09 | 2018-03-27 | 生命技术公司 | 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体 |
| WO2017024173A1 (en) | 2015-08-04 | 2017-02-09 | The Regents Of The University Of Colorado | Multiplexed binary assembly and quantitative tracking of bacterial populations |
| CN108779468A (zh) | 2015-10-29 | 2018-11-09 | 阿迈瑞斯公司 | 生成月桂烯的组合物和方法 |
| CN106811459B (zh) * | 2015-11-27 | 2019-09-27 | 清华大学 | 一种利用短链引物阻遏提高dna克隆及组装效率的方法 |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11293029B2 (en) | 2015-12-07 | 2022-04-05 | Zymergen Inc. | Promoters from Corynebacterium glutamicum |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| EP3458595A2 (en) | 2016-05-18 | 2019-03-27 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| EP3478833A4 (en) | 2016-06-30 | 2019-10-02 | Zymergen, Inc. | METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE |
| WO2018005793A1 (en) | 2016-06-30 | 2018-01-04 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
| GB201621589D0 (en) * | 2016-12-19 | 2017-02-01 | Univ Gent And Katholieke Univ Leuven K U Leuven R&D | Polynucleotide shuffling method |
| WO2018140652A1 (en) | 2017-01-25 | 2018-08-02 | Amyris, Inc. | Co-production of a sesquiterpene and a carotenoid |
| RS63202B1 (sr) | 2017-06-30 | 2022-06-30 | Inscripta Inc | Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| CN111433369A (zh) | 2017-09-18 | 2020-07-17 | 阿迈瑞斯公司 | 用于毕赤酵母和其他宿主细胞基因组整合的方法 |
| WO2019055535A1 (en) | 2017-09-18 | 2019-03-21 | Amyris, Inc. | METHODS OF GENOMIC INTEGRATION FOR HOST CELLS COMPRISING HANSENULA AND ARXULA |
| US11884928B2 (en) | 2017-09-18 | 2024-01-30 | Amyris, Inc. | Methods for genetic engineering Kluyveromyces host cells |
| EP3684927B1 (en) | 2017-09-18 | 2024-05-29 | Amyris, Inc. | Methods for genomic integration for kluyveromyces host cells |
| EP3688167A4 (en) * | 2017-09-29 | 2021-07-14 | Victoria Link Limited | SYSTEM FOR MODULAR DNA ARRANGEMENT |
| CN111372650A (zh) | 2017-09-30 | 2020-07-03 | 因思科瑞普特公司 | 流通式电穿孔仪器 |
| WO2019190874A1 (en) | 2018-03-29 | 2019-10-03 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| AU2019256237A1 (en) * | 2018-04-17 | 2020-10-29 | Nanjing GenScript Biotech Co., Ltd. | DNA construct design system |
| WO2019209926A1 (en) | 2018-04-24 | 2019-10-31 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| IL292273B2 (en) | 2018-08-14 | 2023-10-01 | Inscripta Inc | Devices, modules and methods for improved detection of edited sequences in living cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| MX2021005028A (es) * | 2018-10-31 | 2021-08-24 | Zymergen Inc | Ensamble determinista multiplexado de genotecas de adn. |
| CN113728130B (zh) * | 2019-04-01 | 2024-09-06 | 国立大学法人神户大学 | 嵌合质粒文库的构建方法 |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| HRP20250315T1 (hr) | 2019-07-11 | 2025-06-06 | Clara Foods Co. | Sastav napitka koji sadrži rekombinantni protein ovomukoid |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| JP7473953B2 (ja) * | 2020-03-24 | 2024-04-24 | 国立大学法人 東京大学 | 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ |
| WO2021211972A1 (en) * | 2020-04-16 | 2021-10-21 | Zymergen Inc. | Circular-permuted nucleic acids for homology-directed editing |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| CN112813103A (zh) * | 2021-01-28 | 2021-05-18 | 云舟生物科技（广州）有限公司 | 一种标准品载体及其在测定重组腺相关病毒滴度中的应用 |
| CN113943666B (zh) * | 2021-11-11 | 2023-08-22 | 广西壮族自治区农业科学院 | 一种酿酒酵母工程菌及其应用 |
| CN115261377B (zh) * | 2021-12-27 | 2024-11-19 | 天津大学 | 一种dna串联重复单元的拆分组装方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5057419A (en) * | 1988-09-22 | 1991-10-15 | Rutgers University | Genetically engineered plasmid and organisms for the production of specialized oils |
| US5976846A (en) * | 1996-01-13 | 1999-11-02 | Passmore; Steven E. | Method for multifragment in vivo cloning and mutation mapping |
| AU9577998A (en) * | 1997-09-29 | 1999-04-23 | City Of Hope | Efficient linking of nucleic acid segments |
| EP1196554B1 (en) * | 1999-03-18 | 2009-02-25 | Complete Genomics AS | Methods of cloning and producing fragment chains with readable information content |
| CA2317865A1 (en) | 2000-03-07 | 2001-09-07 | University Of Guelph | Dna joining method |
| EP1313851B1 (en) * | 2000-08-21 | 2006-01-04 | Invitrogen Corporation | Methods and reagents for molecular cloning |
| US20030087254A1 (en) * | 2001-04-05 | 2003-05-08 | Simon Delagrave | Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics |
| US20020165175A1 (en) * | 2001-04-17 | 2002-11-07 | Xiaowu Liang | Fast and enzymeless cloning of nucleic acid fragments |
| CN1263860C (zh) * | 2002-09-30 | 2006-07-12 | 华南农业大学 | 多基因载体的构建方法与应用 |
| US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| US7776532B2 (en) * | 2005-08-11 | 2010-08-17 | Synthetic Genomics, Inc. | Method for in vitro recombination |
| EP1915446B1 (en) * | 2005-08-11 | 2017-06-14 | Synthetic Genomics, Inc. | In vitro recombination method |
| EP2078077A2 (en) | 2006-10-04 | 2009-07-15 | Codon Devices, Inc | Nucleic acid libraries and their design and assembly |
| EP2115144A1 (en) * | 2007-02-05 | 2009-11-11 | Philipps-Universität Marburg | Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases |
| US9593329B2 (en) | 2007-10-08 | 2017-03-14 | Synthetic Genomics, Inc. | Assembly of large nucleic acids |
| WO2010059763A1 (en) * | 2008-11-19 | 2010-05-27 | Amyris Biotechnologies, Inc. | Compositions and methods for the assembly of polynucleotides |
-
2009
- 2009-11-19 WO PCT/US2009/065048 patent/WO2010059763A1/en not_active Ceased
- 2009-11-19 MX MX2011005195A patent/MX2011005195A/es active IP Right Grant
- 2009-11-19 CA CA2744153A patent/CA2744153C/en active Active
- 2009-11-19 BR BRPI0922187-5A patent/BRPI0922187B1/pt active IP Right Grant
- 2009-11-19 US US12/622,401 patent/US8221982B2/en active Active
- 2009-11-19 KR KR1020117014144A patent/KR101794298B1/ko not_active Expired - Fee Related
- 2009-11-19 JP JP2011537596A patent/JP2012509084A/ja active Pending
- 2009-11-19 SG SG2011036274A patent/SG171760A1/en unknown
- 2009-11-19 CN CN200980154897.0A patent/CN102282255B/zh not_active Expired - Fee Related
- 2009-11-19 AU AU2009316660A patent/AU2009316660B2/en not_active Ceased
- 2009-11-19 EP EP09764127.8A patent/EP2358875B1/en active Active
-
2010
- 2010-01-08 US US12/684,874 patent/US8110360B2/en active Active
-
2011
- 2011-05-17 ZA ZA2011/03637A patent/ZA201103637B/en unknown
-
2012
- 2012-03-26 US US13/430,322 patent/US8546136B2/en active Active
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