CN102282255B - 多核苷酸装配的组合物和方法 - Google Patents

多核苷酸装配的组合物和方法 Download PDF

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CN102282255B
CN102282255B CN200980154897.0A CN200980154897A CN102282255B CN 102282255 B CN102282255 B CN 102282255B CN 200980154897 A CN200980154897 A CN 200980154897A CN 102282255 B CN102282255 B CN 102282255B
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nucleic acid
sequence
restriction enzyme
dna
acid molecule
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CN102282255A (zh
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扎克·塞贝
雷蒙德·洛
杰弗里·A·尤伯萨克斯
苏尼尔·S·钱德兰
埃里克·杰迪戴亚·迪安
达伦·M·普拉特
肯尼思·俊树·竹冈
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Amyris Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN200980154897.0A 2008-11-19 2009-11-19 多核苷酸装配的组合物和方法 Expired - Fee Related CN102282255B (zh)

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Application Number Priority Date Filing Date Title
US11610908P 2008-11-19 2008-11-19
US61/116,109 2008-11-19
US16223009P 2009-03-20 2009-03-20
US61/162,230 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

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CN102282255B true CN102282255B (zh) 2014-05-07

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US (3) US8221982B2 (enExample)
EP (1) EP2358875B1 (enExample)
JP (1) JP2012509084A (enExample)
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CN (1) CN102282255B (enExample)
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BR (1) BRPI0922187B1 (enExample)
CA (1) CA2744153C (enExample)
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CN102282255A (zh) 2011-12-14
JP2012509084A (ja) 2012-04-19
CA2744153C (en) 2020-07-07
KR101794298B1 (ko) 2017-11-20
SG171760A1 (en) 2011-07-28
BRPI0922187B1 (pt) 2021-05-04
US8221982B2 (en) 2012-07-17
WO2010059763A1 (en) 2010-05-27
US20120245056A1 (en) 2012-09-27
MX2011005195A (es) 2011-09-01
AU2009316660A1 (en) 2011-07-07
US20100136633A1 (en) 2010-06-03
BRPI0922187A2 (pt) 2018-10-23
KR20110102351A (ko) 2011-09-16
AU2009316660B2 (en) 2015-01-29
EP2358875B1 (en) 2015-08-12
CA2744153A1 (en) 2010-05-27
EP2358875A1 (en) 2011-08-24
US20100124768A1 (en) 2010-05-20
US8546136B2 (en) 2013-10-01
ZA201103637B (en) 2012-08-29
US8110360B2 (en) 2012-02-07

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