ZA201103637B - Compositions and methods for the assembly of polynucleotides - Google Patents

Compositions and methods for the assembly of polynucleotides

Info

Publication number
ZA201103637B
ZA201103637B ZA2011/03637A ZA201103637A ZA201103637B ZA 201103637 B ZA201103637 B ZA 201103637B ZA 2011/03637 A ZA2011/03637 A ZA 2011/03637A ZA 201103637 A ZA201103637 A ZA 201103637A ZA 201103637 B ZA201103637 B ZA 201103637B
Authority
ZA
South Africa
Prior art keywords
polynucleotides
compositions
assembly
methods
Prior art date
Application number
ZA2011/03637A
Other languages
English (en)
Inventor
Zach Serber
Raymond Lowe
Jeffrey A Ubersax
Sunil S Chandran
Erik Jedediah Dean
Darren M Platt
Kenneth Toshiki Takeoka
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of ZA201103637B publication Critical patent/ZA201103637B/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ZA2011/03637A 2008-11-19 2011-05-17 Compositions and methods for the assembly of polynucleotides ZA201103637B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11610908P 2008-11-19 2008-11-19
US16223009P 2009-03-20 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

Publications (1)

Publication Number Publication Date
ZA201103637B true ZA201103637B (en) 2012-08-29

Family

ID=41698076

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2011/03637A ZA201103637B (en) 2008-11-19 2011-05-17 Compositions and methods for the assembly of polynucleotides

Country Status (12)

Country Link
US (3) US8221982B2 (enExample)
EP (1) EP2358875B1 (enExample)
JP (1) JP2012509084A (enExample)
KR (1) KR101794298B1 (enExample)
CN (1) CN102282255B (enExample)
AU (1) AU2009316660B2 (enExample)
BR (1) BRPI0922187B1 (enExample)
CA (1) CA2744153C (enExample)
MX (1) MX2011005195A (enExample)
SG (1) SG171760A1 (enExample)
WO (1) WO2010059763A1 (enExample)
ZA (1) ZA201103637B (enExample)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009316660B2 (en) * 2008-11-19 2015-01-29 Amrys, Inc. Compositions and methods for the assembly of polynucleotides
US7919605B1 (en) 2010-08-30 2011-04-05 Amyris, Inc. Nucleic acids, compositions and methods for the excision of target nucleic acids
WO2012149470A1 (en) 2011-04-27 2012-11-01 Amyris, Inc. Methods for genomic modification
KR102021914B1 (ko) 2011-11-09 2019-09-17 아미리스 인코퍼레이티드 아세틸-코엔자임 a로부터 유래되는 이소프레노이드의 제조 방법
US8332160B1 (en) * 2011-11-17 2012-12-11 Amyris Biotechnologies, Inc. Systems and methods for engineering nucleic acid constructs using scoring techniques
AU2013203380A1 (en) * 2012-01-13 2013-08-01 Chromatin, Inc. Engineering plants with rate limiting farnesene metabolic genes
DK2831238T3 (en) * 2012-03-27 2018-04-03 Dsm Ip Assets Bv CLONING PROCEDURE
CN104508130B (zh) * 2012-06-29 2016-09-28 麻省理工学院 大规模并行组合遗传学
WO2014092886A2 (en) * 2012-12-10 2014-06-19 Agilent Technologies, Inc. Pairing code directed assembly
CN105189772B (zh) 2013-03-15 2019-09-13 阿迈瑞斯公司 磷酸转酮酶和磷酸转乙酰酶生产乙酰-辅酶a衍生化合物的用途
EP2986635B1 (en) 2013-04-18 2018-10-03 Fondazione Telethon Effective delivery of large genes by dual aav vectors
AU2014368982B2 (en) 2013-12-19 2021-03-25 Amyris, Inc. Methods for genomic integration
WO2015120403A1 (en) 2014-02-08 2015-08-13 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking pcr
WO2015184016A2 (en) * 2014-05-27 2015-12-03 The Broad Institute, Inc. High-thoughput assembly of genetic elements
EP3217807A4 (en) 2014-11-11 2018-09-12 Clara Foods Co. Methods and compositions for egg white protein production
US20180127804A1 (en) 2014-12-05 2018-05-10 Amyris, Inc. High-throughput sequencing of polynucleotides
US20180327779A1 (en) * 2015-03-03 2018-11-15 Fondazione Telethon (IT/IT) Multiple vector system and uses thereof
JP6698708B2 (ja) 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
US10793851B2 (en) 2015-08-04 2020-10-06 The Regents Of The University Of Colorado, A Body Corporate Multiplexed binary assembly and quantitative tracking of bacterial populations
WO2017075538A1 (en) 2015-10-29 2017-05-04 Amyris, Inc. Compositions and methods for production of myrcene
CN106811459B (zh) * 2015-11-27 2019-09-27 清华大学 一种利用短链引物阻遏提高dna克隆及组装效率的方法
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
CA3007635A1 (en) 2015-12-07 2017-06-15 Zymergen Inc. Promoters from corynebacterium glutamicum
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
JP2019519242A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド 細菌ヘモグロビンライブラリーを生成するための方法およびその使用
WO2018005793A1 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
GB201621589D0 (en) * 2016-12-19 2017-02-01 Univ Gent And Katholieke Univ Leuven K U Leuven R&D Polynucleotide shuffling method
WO2018140652A1 (en) 2017-01-25 2018-08-02 Amyris, Inc. Co-production of a sesquiterpene and a carotenoid
HUE058668T2 (hu) 2017-06-30 2022-09-28 Inscripta Inc Automatizált sejtkezelési eljárások, modulok, berendezések és rendszerek
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019190590A2 (en) 2017-09-18 2019-10-03 Amyris, Inc. Methods for genomic integration for kluyveromyces host cells
EP3684939A1 (en) 2017-09-18 2020-07-29 Amyris, Inc. Methods for genomic integration in pichia and other host cells
WO2019055535A1 (en) 2017-09-18 2019-03-21 Amyris, Inc. METHODS OF GENOMIC INTEGRATION FOR HOST CELLS COMPRISING HANSENULA AND ARXULA
EP3684940A1 (en) 2017-09-18 2020-07-29 Amyris, Inc. Methods for genetic engineering kluyveromyces host cells
US11655476B2 (en) * 2017-09-29 2023-05-23 Victoria Link Llimited Modular DNA assembly system
WO2019068022A1 (en) 2017-09-30 2019-04-04 Inscripta, Inc. CONTINUOUS FLOW ELECTROPORATION INSTRUMENTATION
WO2019190874A1 (en) 2018-03-29 2019-10-03 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
EP3782057B1 (en) * 2018-04-17 2025-06-25 Nanjing GenScript Biotech Co., Ltd. Dna construct design system
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11965154B2 (en) 2018-08-30 2024-04-23 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
SG11202104409YA (en) * 2018-10-31 2021-05-28 Zymergen Inc Multiplexed deterministic assembly of dna libraries
CA3135856A1 (en) * 2019-04-01 2020-10-08 National University Corporation Kobe University Method for constructing chimeric plasmid library
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
SI3997118T1 (sl) 2019-07-11 2025-06-30 Clara Foods Co. Sestavek napitka, ki obsega rekombinantni ovomukoidni protein
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
JP7473953B2 (ja) * 2020-03-24 2024-04-24 国立大学法人 東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ
WO2021211972A1 (en) * 2020-04-16 2021-10-21 Zymergen Inc. Circular-permuted nucleic acids for homology-directed editing
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CN112813103A (zh) * 2021-01-28 2021-05-18 云舟生物科技(广州)有限公司 一种标准品载体及其在测定重组腺相关病毒滴度中的应用
CN113943666B (zh) * 2021-11-11 2023-08-22 广西壮族自治区农业科学院 一种酿酒酵母工程菌及其应用
CN115261377B (zh) * 2021-12-27 2024-11-19 天津大学 一种dna串联重复单元的拆分组装方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5057419A (en) * 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
ATE361377T1 (de) 1997-09-29 2007-05-15 Hope City Leistungstarke verknüpfung von nukleinsäuresegmenten
JP2004512807A (ja) * 1999-03-18 2004-04-30 コンプリート ゲノミックス エイエス 可読情報を有するフラグメント鎖のクローニングおよび生成方法
CA2317865A1 (en) 2000-03-07 2001-09-07 University Of Guelph Dna joining method
ATE315086T1 (de) * 2000-08-21 2006-02-15 Invitrogen Corp Methoden und reagenzien für molekulares klonieren
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
US20020165175A1 (en) 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
CN1263860C (zh) * 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
US20080050808A1 (en) * 2002-10-09 2008-02-28 Reed Thomas D DNA modular cloning vector plasmids and methods for their use
EP1915446B1 (en) * 2005-08-11 2017-06-14 Synthetic Genomics, Inc. In vitro recombination method
MY144014A (en) * 2005-08-11 2011-07-29 Synthetic Genomics Inc Method for in vitro recombination
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly
US20100291633A1 (en) * 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
WO2009048885A2 (en) * 2007-10-08 2009-04-16 Synthetic Genomics, Inc. Assembly of large nucleic acids
AU2009316660B2 (en) * 2008-11-19 2015-01-29 Amrys, Inc. Compositions and methods for the assembly of polynucleotides

Also Published As

Publication number Publication date
US20100136633A1 (en) 2010-06-03
CN102282255B (zh) 2014-05-07
WO2010059763A1 (en) 2010-05-27
CA2744153C (en) 2020-07-07
JP2012509084A (ja) 2012-04-19
CA2744153A1 (en) 2010-05-27
EP2358875A1 (en) 2011-08-24
US8110360B2 (en) 2012-02-07
AU2009316660A1 (en) 2011-07-07
AU2009316660B2 (en) 2015-01-29
BRPI0922187B1 (pt) 2021-05-04
EP2358875B1 (en) 2015-08-12
BRPI0922187A2 (pt) 2018-10-23
CN102282255A (zh) 2011-12-14
KR101794298B1 (ko) 2017-11-20
US20120245056A1 (en) 2012-09-27
MX2011005195A (es) 2011-09-01
SG171760A1 (en) 2011-07-28
US8546136B2 (en) 2013-10-01
US8221982B2 (en) 2012-07-17
KR20110102351A (ko) 2011-09-16
US20100124768A1 (en) 2010-05-20

Similar Documents

Publication Publication Date Title
IL210153A0 (en) Nutrigenomics methods and compositions
ZA201003084B (en) Compositions and methods for enhancing cognitive function
ZA201001300B (en) Antigen-asjuvant compositions and methods
LT2634231T (lt) Sudėtys
GB0810404D0 (en) Compositions
IL212822A0 (en) Anti-cxcr1 compositions and methods
GB0819530D0 (en) Methods and compositions
GB0812041D0 (en) Compositions
IL210559A0 (en) Novel compositions and methods
GB0808537D0 (en) Compositions
GB0818804D0 (en) Compositions
GB0721291D0 (en) Methods and compositions
SG10201505149WA (en) Novel compositions and methods
GB0817585D0 (en) Novel compositions and methods
GB0719526D0 (en) Compositions and methods
SI2320888T1 (sl) Sestavki laidlomicina in postopki
ZA201100008B (en) Anti-tumour compositions and methods
GB0811250D0 (en) Methods and compositions
GB0818399D0 (en) Methods and compositions
GB0811152D0 (en) Methods and compositions
GB0803464D0 (en) Methods and compositions
GB0816534D0 (en) Compositions and methods
GB0724549D0 (en) Compositions and methods
GB0723775D0 (en) Methods and compositions
GB0717683D0 (en) Compositions and methods