JP2012509084A - ポリヌクレオチドアセンブリに関する組成物及び方法 - Google Patents

ポリヌクレオチドアセンブリに関する組成物及び方法 Download PDF

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JP2012509084A
JP2012509084A JP2011537596A JP2011537596A JP2012509084A JP 2012509084 A JP2012509084 A JP 2012509084A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2012509084 A JP2012509084 A JP 2012509084A
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nucleic acid
annealable linker
restriction site
restriction
linker sequence
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JP2012509084A5 (enExample
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セルベル ザクフ
ロウエ ライモンド
エー. ウベルサク ジェエフフレイ
エス. チャンドラン スニル
ジェエデドイアフ デアン エリク
エム. プルアトト ダルレン
トシキ タケオカ ケンネトフ
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アムイリス, インコーポレイテッド
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2011537596A 2008-11-19 2009-11-19 ポリヌクレオチドアセンブリに関する組成物及び方法 Pending JP2012509084A (ja)

Applications Claiming Priority (5)

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US11610908P 2008-11-19 2008-11-19
US61/116,109 2008-11-19
US16223009P 2009-03-20 2009-03-20
US61/162,230 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

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JP2012509084A5 JP2012509084A5 (enExample) 2012-10-11

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US (3) US8221982B2 (enExample)
EP (1) EP2358875B1 (enExample)
JP (1) JP2012509084A (enExample)
KR (1) KR101794298B1 (enExample)
CN (1) CN102282255B (enExample)
AU (1) AU2009316660B2 (enExample)
BR (1) BRPI0922187B1 (enExample)
CA (1) CA2744153C (enExample)
MX (1) MX2011005195A (enExample)
SG (1) SG171760A1 (enExample)
WO (1) WO2010059763A1 (enExample)
ZA (1) ZA201103637B (enExample)

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JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
JP2021151200A (ja) * 2020-03-24 2021-09-30 国立大学法人 東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ

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US10287627B2 (en) 2014-02-08 2019-05-14 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking PCR
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KR20180084756A (ko) 2015-12-07 2018-07-25 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터
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Cited By (4)

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Publication number Priority date Publication date Assignee Title
JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
JP2021151200A (ja) * 2020-03-24 2021-09-30 国立大学法人 東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ
WO2021192596A1 (ja) * 2020-03-24 2021-09-30 国立大学法人東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ
JP7473953B2 (ja) 2020-03-24 2024-04-24 国立大学法人 東京大学 連結dnaの製造方法及びそれに用いるためのベクターの組み合わせ

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CN102282255A (zh) 2011-12-14
CA2744153C (en) 2020-07-07
KR101794298B1 (ko) 2017-11-20
SG171760A1 (en) 2011-07-28
BRPI0922187B1 (pt) 2021-05-04
US8221982B2 (en) 2012-07-17
WO2010059763A1 (en) 2010-05-27
US20120245056A1 (en) 2012-09-27
MX2011005195A (es) 2011-09-01
AU2009316660A1 (en) 2011-07-07
US20100136633A1 (en) 2010-06-03
BRPI0922187A2 (pt) 2018-10-23
KR20110102351A (ko) 2011-09-16
AU2009316660B2 (en) 2015-01-29
EP2358875B1 (en) 2015-08-12
CN102282255B (zh) 2014-05-07
CA2744153A1 (en) 2010-05-27
EP2358875A1 (en) 2011-08-24
US20100124768A1 (en) 2010-05-20
US8546136B2 (en) 2013-10-01
ZA201103637B (en) 2012-08-29
US8110360B2 (en) 2012-02-07

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