JP2012509084A - ポリヌクレオチドアセンブリに関する組成物及び方法 - Google Patents

ポリヌクレオチドアセンブリに関する組成物及び方法 Download PDF

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JP2012509084A
JP2012509084A JP2011537596A JP2011537596A JP2012509084A JP 2012509084 A JP2012509084 A JP 2012509084A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2012509084 A JP2012509084 A JP 2012509084A
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nucleic acid
annealable linker
restriction site
restriction
linker sequence
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JP2012509084A5 (enExample
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セルベル ザクフ
ロウエ ライモンド
エー． ウベルサク ジェエフフレイ
エス． チャンドラン スニル
ジェエデドイアフ デアン エリク
エム． プルアトト ダルレン
トシキ タケオカ ケンネトフ
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アムイリス， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2011537596A 2008-11-19 2009-11-19 ポリヌクレオチドアセンブリに関する組成物及び方法 Pending JP2012509084A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11610908P 2008-11-19 2008-11-19
US61/116,109 2008-11-19
US16223009P 2009-03-20 2009-03-20
US61/162,230 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

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JP2012509084A true JP2012509084A (ja) 2012-04-19
JP2012509084A5 JP2012509084A5 (enExample) 2012-10-11

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US (3) US8221982B2 (enExample)
EP (1) EP2358875B1 (enExample)
JP (1) JP2012509084A (enExample)
KR (1) KR101794298B1 (enExample)
CN (1) CN102282255B (enExample)
AU (1) AU2009316660B2 (enExample)
BR (1) BRPI0922187B1 (enExample)
CA (1) CA2744153C (enExample)
MX (1) MX2011005195A (enExample)
SG (1) SG171760A1 (enExample)
WO (1) WO2010059763A1 (enExample)
ZA (1) ZA201103637B (enExample)

Cited By (2)

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JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
WO2021192596A1 (ja) * 2020-03-24 2021-09-30 国立大学法人東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ

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US8332160B1 (en) 2011-11-17 2012-12-11 Amyris Biotechnologies, Inc. Systems and methods for engineering nucleic acid constructs using scoring techniques
WO2013106868A1 (en) * 2012-01-13 2013-07-18 Chromatin, Inc. Engineering plants with rate limiting farnesene metabolic genes
WO2013144257A1 (en) * 2012-03-27 2013-10-03 Dsm Ip Assets B.V. Cloning method
US9976162B2 (en) 2012-12-10 2018-05-22 Agilent Technologies, Inc. Pairing code directed assembly
BR112015023089A2 (pt) 2013-03-15 2017-11-21 Amyris Inc célula hospedeira, método para produzir um isoprenoide, e método para aumentar a produção de acetil-coa ou um composto derivado de acetil-coa
DK2986635T3 (en) 2013-04-18 2019-01-28 Fond Telethon EFFECTIVE DELIVERY OF BIG GENES THROUGH DUAL-AAV VECTORS
US9476065B2 (en) 2013-12-19 2016-10-25 Amyris, Inc. Methods for genomic integration
US10287627B2 (en) 2014-02-08 2019-05-14 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking PCR
WO2015184016A2 (en) * 2014-05-27 2015-12-03 The Broad Institute, Inc. High-thoughput assembly of genetic elements
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
US20180127804A1 (en) 2014-12-05 2018-05-10 Amyris, Inc. High-throughput sequencing of polynucleotides
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WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10793851B2 (en) 2015-08-04 2020-10-06 The Regents Of The University Of Colorado, A Body Corporate Multiplexed binary assembly and quantitative tracking of bacterial populations
EP3368673B1 (en) 2015-10-29 2020-07-29 Amyris, Inc. Compositions and methods for production of myrcene
CN106811459B (zh) * 2015-11-27 2019-09-27 清华大学 一种利用短链引物阻遏提高dna克隆及组装效率的方法
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
JP6821598B2 (ja) 2015-12-07 2021-01-27 ザイマージェン インコーポレイテッド Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーター
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
EP3478833A4 (en) 2016-06-30 2019-10-02 Zymergen, Inc. METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE
US10544411B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
GB201621589D0 (en) * 2016-12-19 2017-02-01 Univ Gent And Katholieke Univ Leuven K U Leuven R&D Polynucleotide shuffling method
WO2018140652A1 (en) 2017-01-25 2018-08-02 Amyris, Inc. Co-production of a sesquiterpene and a carotenoid
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US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019055495A1 (en) 2017-09-18 2019-03-21 Amyris, Inc. METHODS FOR GENETICALLY MODIFYING KLUYVEROMYCES HOST CELLS
WO2019055481A1 (en) 2017-09-18 2019-03-21 Amyris, Inc. METHODS OF GENOMIC INTEGRATION IN PICHIA AND OTHER HOST CELLS
US11685934B2 (en) 2017-09-18 2023-06-27 Amyris, Inc. Methods for genomic integration for Kluyveromyces host cells
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EP3688167A4 (en) * 2017-09-29 2021-07-14 Victoria Link Limited SYSTEM FOR MODULAR DNA ARRANGEMENT
US10435713B2 (en) 2017-09-30 2019-10-08 Inscripta, Inc. Flow through electroporation instrumentation
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
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US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
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US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
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Cited By (4)

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Publication number Priority date Publication date Assignee Title
JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
WO2021192596A1 (ja) * 2020-03-24 2021-09-30 国立大学法人東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ
JP2021151200A (ja) * 2020-03-24 2021-09-30 国立大学法人 東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ
JP7473953B2 (ja) 2020-03-24 2024-04-24 国立大学法人 東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ

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US20120245056A1 (en) 2012-09-27
SG171760A1 (en) 2011-07-28
EP2358875A1 (en) 2011-08-24
CA2744153C (en) 2020-07-07
US20100124768A1 (en) 2010-05-20
EP2358875B1 (en) 2015-08-12
CN102282255A (zh) 2011-12-14
CA2744153A1 (en) 2010-05-27
WO2010059763A1 (en) 2010-05-27
US8110360B2 (en) 2012-02-07
KR20110102351A (ko) 2011-09-16
CN102282255B (zh) 2014-05-07
ZA201103637B (en) 2012-08-29
BRPI0922187A2 (pt) 2018-10-23
AU2009316660B2 (en) 2015-01-29
BRPI0922187B1 (pt) 2021-05-04
US20100136633A1 (en) 2010-06-03
US8221982B2 (en) 2012-07-17
AU2009316660A1 (en) 2011-07-07
US8546136B2 (en) 2013-10-01
MX2011005195A (es) 2011-09-01
KR101794298B1 (ko) 2017-11-20

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