BRPI0922187B1 - Métodos para gerar um polinucleotídeo e para gerar uma célula hospedeira - Google Patents
Métodos para gerar um polinucleotídeo e para gerar uma célula hospedeira Download PDFInfo
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- BRPI0922187B1 BRPI0922187B1 BRPI0922187-5A BRPI0922187A BRPI0922187B1 BR PI0922187 B1 BRPI0922187 B1 BR PI0922187B1 BR PI0922187 A BRPI0922187 A BR PI0922187A BR PI0922187 B1 BRPI0922187 B1 BR PI0922187B1
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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| PCT/US2009/065048 WO2010059763A1 (en) | 2008-11-19 | 2009-11-19 | Compositions and methods for the assembly of polynucleotides |
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