BRPI0922187B1 - Métodos para gerar um polinucleotídeo e para gerar uma célula hospedeira - Google Patents

Métodos para gerar um polinucleotídeo e para gerar uma célula hospedeira Download PDF

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BRPI0922187B1
BRPI0922187B1 BRPI0922187-5A BRPI0922187A BRPI0922187B1 BR PI0922187 B1 BRPI0922187 B1 BR PI0922187B1 BR PI0922187 A BRPI0922187 A BR PI0922187A BR PI0922187 B1 BRPI0922187 B1 BR PI0922187B1
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nucleic acid
linker sequence
polynucleotide
dna
sequence
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BRPI0922187-5A
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English (en)
Portuguese (pt)
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Zach Serber
Raymond Lowe
Jeffrey A. Ubersax
Sunil S. Chandran
Erick Jedediah Dean
Darren M. Platt
Kenneth Toshiki Takeoka
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Amyris, Inc
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Publication of BRPI0922187A2 publication Critical patent/BRPI0922187A2/pt
Publication of BRPI0922187B1 publication Critical patent/BRPI0922187B1/pt

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BRPI0922187-5A 2008-11-19 2009-11-19 Métodos para gerar um polinucleotídeo e para gerar uma célula hospedeira BRPI0922187B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11610908P 2008-11-19 2008-11-19
US61/116,109 2008-11-19
US16223009P 2009-03-20 2009-03-20
US61/162,230 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

Publications (2)

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BRPI0922187A2 BRPI0922187A2 (pt) 2018-10-23
BRPI0922187B1 true BRPI0922187B1 (pt) 2021-05-04

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BRPI0922187-5A BRPI0922187B1 (pt) 2008-11-19 2009-11-19 Métodos para gerar um polinucleotídeo e para gerar uma célula hospedeira

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US (3) US8221982B2 (enExample)
EP (1) EP2358875B1 (enExample)
JP (1) JP2012509084A (enExample)
KR (1) KR101794298B1 (enExample)
CN (1) CN102282255B (enExample)
AU (1) AU2009316660B2 (enExample)
BR (1) BRPI0922187B1 (enExample)
CA (1) CA2744153C (enExample)
MX (1) MX2011005195A (enExample)
SG (1) SG171760A1 (enExample)
WO (1) WO2010059763A1 (enExample)
ZA (1) ZA201103637B (enExample)

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Also Published As

Publication number Publication date
KR101794298B1 (ko) 2017-11-20
US8221982B2 (en) 2012-07-17
US20100136633A1 (en) 2010-06-03
AU2009316660B2 (en) 2015-01-29
EP2358875B1 (en) 2015-08-12
EP2358875A1 (en) 2011-08-24
ZA201103637B (en) 2012-08-29
CN102282255B (zh) 2014-05-07
US20120245056A1 (en) 2012-09-27
SG171760A1 (en) 2011-07-28
BRPI0922187A2 (pt) 2018-10-23
AU2009316660A1 (en) 2011-07-07
US8546136B2 (en) 2013-10-01
US8110360B2 (en) 2012-02-07
JP2012509084A (ja) 2012-04-19
WO2010059763A1 (en) 2010-05-27
CA2744153C (en) 2020-07-07
CN102282255A (zh) 2011-12-14
KR20110102351A (ko) 2011-09-16
MX2011005195A (es) 2011-09-01
US20100124768A1 (en) 2010-05-20
CA2744153A1 (en) 2010-05-27

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