MX2011005195A - Composiciones y metodos para el ensamblaje de polinucleotidos. - Google Patents
Composiciones y metodos para el ensamblaje de polinucleotidos.Info
- Publication number
- MX2011005195A MX2011005195A MX2011005195A MX2011005195A MX2011005195A MX 2011005195 A MX2011005195 A MX 2011005195A MX 2011005195 A MX2011005195 A MX 2011005195A MX 2011005195 A MX2011005195 A MX 2011005195A MX 2011005195 A MX2011005195 A MX 2011005195A
- Authority
- MX
- Mexico
- Prior art keywords
- polynucleotides
- annealable linker
- methods
- assembly
- dna
- Prior art date
Links
- 102000040430 polynucleotide Human genes 0.000 title abstract 4
- 108091033319 polynucleotide Proteins 0.000 title abstract 4
- 239000002157 polynucleotide Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 230000006801 homologous recombination Effects 0.000 abstract 1
- 238000002744 homologous recombination Methods 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 238000007858 polymerase cycling assembly Methods 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11610908P | 2008-11-19 | 2008-11-19 | |
| US16223009P | 2009-03-20 | 2009-03-20 | |
| PCT/US2009/065048 WO2010059763A1 (en) | 2008-11-19 | 2009-11-19 | Compositions and methods for the assembly of polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2011005195A true MX2011005195A (es) | 2011-09-01 |
Family
ID=41698076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2011005195A MX2011005195A (es) | 2008-11-19 | 2009-11-19 | Composiciones y metodos para el ensamblaje de polinucleotidos. |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US8221982B2 (enExample) |
| EP (1) | EP2358875B1 (enExample) |
| JP (1) | JP2012509084A (enExample) |
| KR (1) | KR101794298B1 (enExample) |
| CN (1) | CN102282255B (enExample) |
| AU (1) | AU2009316660B2 (enExample) |
| BR (1) | BRPI0922187B1 (enExample) |
| CA (1) | CA2744153C (enExample) |
| MX (1) | MX2011005195A (enExample) |
| SG (1) | SG171760A1 (enExample) |
| WO (1) | WO2010059763A1 (enExample) |
| ZA (1) | ZA201103637B (enExample) |
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| WO2010059763A1 (en) * | 2008-11-19 | 2010-05-27 | Amyris Biotechnologies, Inc. | Compositions and methods for the assembly of polynucleotides |
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| ES2663424T3 (es) * | 2012-06-29 | 2018-04-12 | Massachusetts Institute Of Technology | Genética combinatoria masivamente en paralelo |
| WO2014092886A2 (en) | 2012-12-10 | 2014-06-19 | Agilent Technologies, Inc. | Pairing code directed assembly |
| EP2971027B1 (en) | 2013-03-15 | 2019-01-30 | Amyris, Inc. | Use of phosphoketolase and phosphotransacetylase for production of acetyl-coenzyme a derived compounds |
| DK2986635T3 (en) | 2013-04-18 | 2019-01-28 | Fond Telethon | EFFECTIVE DELIVERY OF BIG GENES THROUGH DUAL-AAV VECTORS |
| US9476065B2 (en) | 2013-12-19 | 2016-10-25 | Amyris, Inc. | Methods for genomic integration |
| US10287627B2 (en) | 2014-02-08 | 2019-05-14 | The Regents Of The University Of Colorado, A Body Corporate | Multiplexed linking PCR |
| WO2015184016A2 (en) * | 2014-05-27 | 2015-12-03 | The Broad Institute, Inc. | High-thoughput assembly of genetic elements |
| CN114365792A (zh) | 2014-11-11 | 2022-04-19 | 克莱拉食品公司 | 用于生成卵清蛋白的方法和组合物 |
| WO2016090266A1 (en) | 2014-12-05 | 2016-06-09 | Amyris, Inc. | High-throughput sequencing of polynucleotides |
| US20180327779A1 (en) * | 2015-03-03 | 2018-11-15 | Fondazione Telethon (IT/IT) | Multiple vector system and uses thereof |
| CN107849600A (zh) | 2015-06-09 | 2018-03-27 | 生命技术公司 | 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体 |
| WO2017024173A1 (en) | 2015-08-04 | 2017-02-09 | The Regents Of The University Of Colorado | Multiplexed binary assembly and quantitative tracking of bacterial populations |
| CN108779468A (zh) | 2015-10-29 | 2018-11-09 | 阿迈瑞斯公司 | 生成月桂烯的组合物和方法 |
| CN106811459B (zh) * | 2015-11-27 | 2019-09-27 | 清华大学 | 一种利用短链引物阻遏提高dna克隆及组装效率的方法 |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11293029B2 (en) | 2015-12-07 | 2022-04-05 | Zymergen Inc. | Promoters from Corynebacterium glutamicum |
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| EP3458595A2 (en) | 2016-05-18 | 2019-03-27 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| EP3478833A4 (en) | 2016-06-30 | 2019-10-02 | Zymergen, Inc. | METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE |
| WO2018005793A1 (en) | 2016-06-30 | 2018-01-04 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
| GB201621589D0 (en) * | 2016-12-19 | 2017-02-01 | Univ Gent And Katholieke Univ Leuven K U Leuven R&D | Polynucleotide shuffling method |
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| RS63202B1 (sr) | 2017-06-30 | 2022-06-30 | Inscripta Inc | Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija |
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| CN111433369A (zh) | 2017-09-18 | 2020-07-17 | 阿迈瑞斯公司 | 用于毕赤酵母和其他宿主细胞基因组整合的方法 |
| WO2019055535A1 (en) | 2017-09-18 | 2019-03-21 | Amyris, Inc. | METHODS OF GENOMIC INTEGRATION FOR HOST CELLS COMPRISING HANSENULA AND ARXULA |
| US11884928B2 (en) | 2017-09-18 | 2024-01-30 | Amyris, Inc. | Methods for genetic engineering Kluyveromyces host cells |
| EP3684927B1 (en) | 2017-09-18 | 2024-05-29 | Amyris, Inc. | Methods for genomic integration for kluyveromyces host cells |
| EP3688167A4 (en) * | 2017-09-29 | 2021-07-14 | Victoria Link Limited | SYSTEM FOR MODULAR DNA ARRANGEMENT |
| CN111372650A (zh) | 2017-09-30 | 2020-07-03 | 因思科瑞普特公司 | 流通式电穿孔仪器 |
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| AU2019256237A1 (en) * | 2018-04-17 | 2020-10-29 | Nanjing GenScript Biotech Co., Ltd. | DNA construct design system |
| WO2019209926A1 (en) | 2018-04-24 | 2019-10-31 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| IL292273B2 (en) | 2018-08-14 | 2023-10-01 | Inscripta Inc | Devices, modules and methods for improved detection of edited sequences in living cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| MX2021005028A (es) * | 2018-10-31 | 2021-08-24 | Zymergen Inc | Ensamble determinista multiplexado de genotecas de adn. |
| CN113728130B (zh) * | 2019-04-01 | 2024-09-06 | 国立大学法人神户大学 | 嵌合质粒文库的构建方法 |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
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| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| HRP20250315T1 (hr) | 2019-07-11 | 2025-06-06 | Clara Foods Co. | Sastav napitka koji sadrži rekombinantni protein ovomukoid |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| JP7473953B2 (ja) * | 2020-03-24 | 2024-04-24 | 国立大学法人 東京大学 | 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ |
| WO2021211972A1 (en) * | 2020-04-16 | 2021-10-21 | Zymergen Inc. | Circular-permuted nucleic acids for homology-directed editing |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| CN112813103A (zh) * | 2021-01-28 | 2021-05-18 | 云舟生物科技（广州）有限公司 | 一种标准品载体及其在测定重组腺相关病毒滴度中的应用 |
| CN113943666B (zh) * | 2021-11-11 | 2023-08-22 | 广西壮族自治区农业科学院 | 一种酿酒酵母工程菌及其应用 |
| CN115261377B (zh) * | 2021-12-27 | 2024-11-19 | 天津大学 | 一种dna串联重复单元的拆分组装方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5057419A (en) * | 1988-09-22 | 1991-10-15 | Rutgers University | Genetically engineered plasmid and organisms for the production of specialized oils |
| US5976846A (en) * | 1996-01-13 | 1999-11-02 | Passmore; Steven E. | Method for multifragment in vivo cloning and mutation mapping |
| AU9577998A (en) * | 1997-09-29 | 1999-04-23 | City Of Hope | Efficient linking of nucleic acid segments |
| EP1196554B1 (en) * | 1999-03-18 | 2009-02-25 | Complete Genomics AS | Methods of cloning and producing fragment chains with readable information content |
| CA2317865A1 (en) | 2000-03-07 | 2001-09-07 | University Of Guelph | Dna joining method |
| EP1313851B1 (en) * | 2000-08-21 | 2006-01-04 | Invitrogen Corporation | Methods and reagents for molecular cloning |
| US20030087254A1 (en) * | 2001-04-05 | 2003-05-08 | Simon Delagrave | Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics |
| US20020165175A1 (en) * | 2001-04-17 | 2002-11-07 | Xiaowu Liang | Fast and enzymeless cloning of nucleic acid fragments |
| CN1263860C (zh) * | 2002-09-30 | 2006-07-12 | 华南农业大学 | 多基因载体的构建方法与应用 |
| US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| US7776532B2 (en) * | 2005-08-11 | 2010-08-17 | Synthetic Genomics, Inc. | Method for in vitro recombination |
| EP1915446B1 (en) * | 2005-08-11 | 2017-06-14 | Synthetic Genomics, Inc. | In vitro recombination method |
| EP2078077A2 (en) | 2006-10-04 | 2009-07-15 | Codon Devices, Inc | Nucleic acid libraries and their design and assembly |
| EP2115144A1 (en) * | 2007-02-05 | 2009-11-11 | Philipps-Universität Marburg | Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases |
| US9593329B2 (en) | 2007-10-08 | 2017-03-14 | Synthetic Genomics, Inc. | Assembly of large nucleic acids |
| WO2010059763A1 (en) * | 2008-11-19 | 2010-05-27 | Amyris Biotechnologies, Inc. | Compositions and methods for the assembly of polynucleotides |
-
2009
- 2009-11-19 WO PCT/US2009/065048 patent/WO2010059763A1/en not_active Ceased
- 2009-11-19 MX MX2011005195A patent/MX2011005195A/es active IP Right Grant
- 2009-11-19 CA CA2744153A patent/CA2744153C/en active Active
- 2009-11-19 BR BRPI0922187-5A patent/BRPI0922187B1/pt active IP Right Grant
- 2009-11-19 US US12/622,401 patent/US8221982B2/en active Active
- 2009-11-19 KR KR1020117014144A patent/KR101794298B1/ko not_active Expired - Fee Related
- 2009-11-19 JP JP2011537596A patent/JP2012509084A/ja active Pending
- 2009-11-19 SG SG2011036274A patent/SG171760A1/en unknown
- 2009-11-19 CN CN200980154897.0A patent/CN102282255B/zh not_active Expired - Fee Related
- 2009-11-19 AU AU2009316660A patent/AU2009316660B2/en not_active Ceased
- 2009-11-19 EP EP09764127.8A patent/EP2358875B1/en active Active
-
2010
- 2010-01-08 US US12/684,874 patent/US8110360B2/en active Active
-
2011
- 2011-05-17 ZA ZA2011/03637A patent/ZA201103637B/en unknown
-
2012
- 2012-03-26 US US13/430,322 patent/US8546136B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20100136633A1 (en) | 2010-06-03 |
| US8110360B2 (en) | 2012-02-07 |
| US20100124768A1 (en) | 2010-05-20 |
| CA2744153A1 (en) | 2010-05-27 |
| ZA201103637B (en) | 2012-08-29 |
| SG171760A1 (en) | 2011-07-28 |
| AU2009316660B2 (en) | 2015-01-29 |
| KR20110102351A (ko) | 2011-09-16 |
| CN102282255A (zh) | 2011-12-14 |
| CA2744153C (en) | 2020-07-07 |
| CN102282255B (zh) | 2014-05-07 |
| EP2358875A1 (en) | 2011-08-24 |
| JP2012509084A (ja) | 2012-04-19 |
| AU2009316660A1 (en) | 2011-07-07 |
| US8221982B2 (en) | 2012-07-17 |
| BRPI0922187B1 (pt) | 2021-05-04 |
| WO2010059763A1 (en) | 2010-05-27 |
| BRPI0922187A2 (pt) | 2018-10-23 |
| US8546136B2 (en) | 2013-10-01 |
| EP2358875B1 (en) | 2015-08-12 |
| US20120245056A1 (en) | 2012-09-27 |
| KR101794298B1 (ko) | 2017-11-20 |
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