JP2012070731A5

JP2012070731A5 -

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Publication number: JP2012070731A5
Authority: JP; Japan
Prior art keywords: stem cells; pluripotent stem; hematopoietic; cells; tgf
Prior art date: 2011-08-31
Legal status : Granted

Application number

JP2011189582A

Other languages

English (en)

Japanese (ja)

Other versions

JP5928681B2 (ja

JP2012070731A (ja

Filing date

2011-08-31

Publication date

2014-10-16

2011-08-31 Application filed filed Critical

2011-08-31 Priority to JP2011189582A priority Critical patent/JP5928681B2/ja

2011-08-31 Priority claimed from JP2011189582A external-priority patent/JP5928681B2/ja

2012-04-12 Publication of JP2012070731A publication Critical patent/JP2012070731A/ja

2014-10-16 Publication of JP2012070731A5 publication Critical patent/JP2012070731A5/ja

2016-06-01 Application granted granted Critical

2016-06-01 Publication of JP5928681B2 publication Critical patent/JP5928681B2/ja

Status Expired - Fee Related legal-status Critical Current

2031-08-31 Anticipated expiration legal-status Critical

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210000003958 hematopoietic stem cell Anatomy 0.000 claims 56
210000001778 pluripotent stem cell Anatomy 0.000 claims 40
230000004069 differentiation Effects 0.000 claims 28
102000004887 Transforming Growth Factor beta Human genes 0.000 claims 24
108090001012 Transforming Growth Factor beta Proteins 0.000 claims 24
239000003112 inhibitor Substances 0.000 claims 24
ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims 24
150000001875 compounds Chemical class 0.000 claims 14
230000001939 inductive effect Effects 0.000 claims 13
238000000034 method Methods 0.000 claims 13
230000001737 promoting effect Effects 0.000 claims 11
230000006698 induction Effects 0.000 claims 9
108091005735 TGF-beta receptors Proteins 0.000 claims 8
102000016715 Transforming Growth Factor beta Receptors Human genes 0.000 claims 8
238000012258 culturing Methods 0.000 claims 8
210000004263 induced pluripotent stem cell Anatomy 0.000 claims 8
238000000338 in vitro Methods 0.000 claims 7
239000003795 chemical substances by application Substances 0.000 claims 5
230000003394 haemopoietic effect Effects 0.000 claims 4
101000762379 Homo sapiens Bone morphogenetic protein 4 Proteins 0.000 claims 3
102000046148 human BMP4 Human genes 0.000 claims 3
101000808011 Homo sapiens Vascular endothelial growth factor A Proteins 0.000 claims 2
102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 2
108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 2
210000004027 cell Anatomy 0.000 claims 2
239000003153 chemical reaction reagent Substances 0.000 claims 2
102000058223 human VEGFA Human genes 0.000 claims 2
102000008137 Bone Morphogenetic Protein 4 Human genes 0.000 claims 1
108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 claims 1
108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 1
102000003974 Fibroblast growth factor 2 Human genes 0.000 claims 1
101001052035 Homo sapiens Fibroblast growth factor 2 Proteins 0.000 claims 1
101000799461 Homo sapiens Thrombopoietin Proteins 0.000 claims 1
108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 claims 1
238000004113 cell culture Methods 0.000 claims 1
210000000130 stem cell Anatomy 0.000 claims 1
IBCXZJCWDGCXQT-UHFFFAOYSA-N c([nH]nc1-c2ccccn2)c1-c1ccnc2c1cccc2 Chemical compound c([nH]nc1-c2ccccn2)c1-c1ccnc2c1cccc2 IBCXZJCWDGCXQT-UHFFFAOYSA-N 0.000 description 2
DKPQHFZUICCZHF-UHFFFAOYSA-N CC(C)(C)c1nc(-c2ccc3nccnc3c2)c(-c2cccc(C)n2)[nH]1 Chemical compound CC(C)(C)c1nc(-c2ccc3nccnc3c2)c(-c2cccc(C)n2)[nH]1 DKPQHFZUICCZHF-UHFFFAOYSA-N 0.000 description 1
FHYUGAJXYORMHI-UHFFFAOYSA-N NC(c(cc1)ccc1-c1nc(-c(cc2)cc3c2OCO3)c(-c2ncccc2)[nH]1)=O Chemical compound NC(c(cc1)ccc1-c1nc(-c(cc2)cc3c2OCO3)c(-c2ncccc2)[nH]1)=O FHYUGAJXYORMHI-UHFFFAOYSA-N 0.000 description 1

JP2011189582A 2010-08-31 2011-08-31 ヒト多能性幹細胞からの造血幹細胞の効率的な誘導方法 Expired - Fee Related JP5928681B2 (ja)