JP2013500003A5 - - Google Patents

Info

Publication number

JP2013500003A5

JP2013500003A5 JP2012520985A JP2012520985A JP2013500003A5 JP 2013500003 A5 JP2013500003 A5 JP 2013500003A5 JP 2012520985 A JP2012520985 A JP 2012520985A JP 2012520985 A JP2012520985 A JP 2012520985A JP 2013500003 A5 JP2013500003 A5 JP 2013500003A5

Authority

JP

Japan

Prior art keywords

myofibroblasts

cells

serum

cell

cell culture

Prior art date

2010-07-02

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 claims 13
• 210000004027 cell Anatomy 0.000 claims 11
• 210000000651 myofibroblast Anatomy 0.000 claims 8
• 238000004113 cell culture Methods 0.000 claims 6
• 239000012679 serum free medium Substances 0.000 claims 5
• 239000012472 biological sample Substances 0.000 claims 4
• 210000002950 fibroblast Anatomy 0.000 claims 4
• JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims 4
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 4
• 239000002609 medium Substances 0.000 claims 3
• 239000000523 sample Substances 0.000 claims 3
• 241000283690 Bos taurus Species 0.000 claims 2
• ZWQVYZXPYSYPJD-RYUDHWBXSA-N Glu-Gly-Phe Chemical compound OC(=O)CC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 ZWQVYZXPYSYPJD-RYUDHWBXSA-N 0.000 claims 2
• 102000004877 Insulin Human genes 0.000 claims 2
• 108090001061 Insulin Proteins 0.000 claims 2
• 206010028980 Neoplasm Diseases 0.000 claims 2
• 239000003242 anti bacterial agent Substances 0.000 claims 2
• 229940088710 antibiotic agent Drugs 0.000 claims 2
• 239000006285 cell suspension Substances 0.000 claims 2
• 238000012258 culturing Methods 0.000 claims 2
• 210000002919 epithelial cell Anatomy 0.000 claims 2
• 229960000890 hydrocortisone Drugs 0.000 claims 2
• 229940125396 insulin Drugs 0.000 claims 2
• 230000001817 pituitary effect Effects 0.000 claims 2
• 210000002966 serum Anatomy 0.000 claims 2
• 239000013589 supplement Substances 0.000 claims 2
• 210000000481 breast Anatomy 0.000 claims 1
• 210000004907 gland Anatomy 0.000 claims 1