AU2010275678B2 - Method for obtaining myofibroblasts - Google Patents

Method for obtaining myofibroblasts Download PDF

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Publication number
AU2010275678B2
AU2010275678B2 AU2010275678A AU2010275678A AU2010275678B2 AU 2010275678 B2 AU2010275678 B2 AU 2010275678B2 AU 2010275678 A AU2010275678 A AU 2010275678A AU 2010275678 A AU2010275678 A AU 2010275678A AU 2010275678 B2 AU2010275678 B2 AU 2010275678B2
Authority
AU
Australia
Prior art keywords
cells
myofibroblasts
serum
medium
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2010275678A
Other languages
English (en)
Other versions
AU2010275678A1 (en
Inventor
Robert Barthel
Nicolas Jacques Rouyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ONCOBIOTEK
Original Assignee
ONCOBIOTEK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ONCOBIOTEK filed Critical ONCOBIOTEK
Publication of AU2010275678A1 publication Critical patent/AU2010275678A1/en
Application granted granted Critical
Publication of AU2010275678B2 publication Critical patent/AU2010275678B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2010275678A 2009-07-24 2010-07-02 Method for obtaining myofibroblasts Ceased AU2010275678B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0955216 2009-07-24
FR0955216A FR2948381B1 (fr) 2009-07-24 2009-07-24 Procede d'obtention de myofibroblastes.
PCT/EP2010/059478 WO2011009706A1 (fr) 2009-07-24 2010-07-02 Procede d'obtention de myofibroblastes

Publications (2)

Publication Number Publication Date
AU2010275678A1 AU2010275678A1 (en) 2012-02-09
AU2010275678B2 true AU2010275678B2 (en) 2016-06-16

Family

ID=41460947

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010275678A Ceased AU2010275678B2 (en) 2009-07-24 2010-07-02 Method for obtaining myofibroblasts

Country Status (12)

Country Link
US (1) US9243228B2 (enExample)
EP (1) EP2456854B1 (enExample)
JP (1) JP5758891B2 (enExample)
CN (1) CN102471761A (enExample)
AU (1) AU2010275678B2 (enExample)
CA (1) CA2767929A1 (enExample)
ES (1) ES2650664T3 (enExample)
FR (1) FR2948381B1 (enExample)
IL (1) IL217352A (enExample)
MX (1) MX2012001004A (enExample)
NZ (1) NZ597728A (enExample)
WO (1) WO2011009706A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096265B (zh) * 2014-07-01 2016-03-23 温州医科大学 一种用于构建人工血管模型的三维收缩模型的制备方法
CN104630134A (zh) * 2015-02-15 2015-05-20 叶中华 阴道上皮细胞培养基及阴道上皮细胞培养方法
CN111500527A (zh) * 2020-06-30 2020-08-07 北京昱龙盛世生物科技有限公司 一种表皮干细胞的分离培养方法
EP4398948A4 (en) * 2021-09-07 2025-07-09 Wisconsin Alumni Res Found CARDIAC FIBROBLAST-DERIVED EXTRACELLULAR MATRIX
CN115197892A (zh) * 2022-06-13 2022-10-18 浙江省人民医院 一种人甲状腺乳头状癌组织中成纤维细胞的分离培养方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573937A (en) * 1989-12-07 1996-11-12 Snow Brand Milk Products Co., Ltd. Serum free culture medium
KR20060114381A (ko) * 2004-02-13 2006-11-06 레프로셀 인코퍼레이티드 배아줄기세포용 피더세포 작성 배지 및 피더세포
JP5523674B2 (ja) * 2005-02-11 2014-06-18 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 植物タンパク質加水分解産物を含有する血清フリーの細胞培養液におけるポリペプチドの生産

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Jester et al (1996) Cornea, vol. 15, pps 505-516 *
Webber et al (1999) Carcinogenesis, vol. 20, pps 1185-1192 *

Also Published As

Publication number Publication date
CN102471761A (zh) 2012-05-23
AU2010275678A1 (en) 2012-02-09
EP2456854A1 (fr) 2012-05-30
EP2456854B1 (fr) 2017-08-30
JP5758891B2 (ja) 2015-08-05
JP2013500003A (ja) 2013-01-07
US20120190109A1 (en) 2012-07-26
MX2012001004A (es) 2012-05-29
FR2948381B1 (fr) 2013-07-12
IL217352A (en) 2016-03-31
ES2650664T3 (es) 2018-01-19
NZ597728A (en) 2013-11-29
FR2948381A1 (fr) 2011-01-28
US9243228B2 (en) 2016-01-26
IL217352A0 (en) 2012-02-29
CA2767929A1 (fr) 2011-01-27
WO2011009706A1 (fr) 2011-01-27

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Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired