NZ597728A - Method for obtaining myofibroblasts - Google Patents

Method for obtaining myofibroblasts

Info

Publication number
NZ597728A
NZ597728A NZ597728A NZ59772810A NZ597728A NZ 597728 A NZ597728 A NZ 597728A NZ 597728 A NZ597728 A NZ 597728A NZ 59772810 A NZ59772810 A NZ 59772810A NZ 597728 A NZ597728 A NZ 597728A
Authority
NZ
New Zealand
Prior art keywords
cells
myofibroblasts
obtaining
sample
obtaining myofibroblasts
Prior art date
Application number
NZ597728A
Inventor
Robert Barthel
Nicolas Jacques Rouyer
Original Assignee
Oncobiotek
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncobiotek filed Critical Oncobiotek
Publication of NZ597728A publication Critical patent/NZ597728A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

597728 Discloses a process for obtainin myofibroblasts comprising: Preparing a sample of cells comprising fibroblasts from a tumor sample and Culturing the cells in a serum-free culture medium for mammary epithelial cells Where the cells obtained in step b) are 80% myfibroblasts.
NZ597728A 2009-07-24 2010-07-02 Method for obtaining myofibroblasts NZ597728A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0955216A FR2948381B1 (en) 2009-07-24 2009-07-24 PROCESS FOR OBTAINING MYOFIBROBLASTS
PCT/EP2010/059478 WO2011009706A1 (en) 2009-07-24 2010-07-02 Method for obtaining myofibroblasts

Publications (1)

Publication Number Publication Date
NZ597728A true NZ597728A (en) 2013-11-29

Family

ID=41460947

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ597728A NZ597728A (en) 2009-07-24 2010-07-02 Method for obtaining myofibroblasts

Country Status (12)

Country Link
US (1) US9243228B2 (en)
EP (1) EP2456854B1 (en)
JP (1) JP5758891B2 (en)
CN (1) CN102471761A (en)
AU (1) AU2010275678B2 (en)
CA (1) CA2767929A1 (en)
ES (1) ES2650664T3 (en)
FR (1) FR2948381B1 (en)
IL (1) IL217352A (en)
MX (1) MX2012001004A (en)
NZ (1) NZ597728A (en)
WO (1) WO2011009706A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096265B (en) * 2014-07-01 2016-03-23 温州医科大学 A kind of preparation method of the three-dimensional shrinkage model for building artificial blood vessel's model
CN104630134A (en) * 2015-02-15 2015-05-20 叶中华 Vaginal epithelial cell culture medium and vaginal epithelial cell culture method
CN111500527A (en) * 2020-06-30 2020-08-07 北京昱龙盛世生物科技有限公司 Separation culture method of epidermal stem cells
EP4398948A1 (en) * 2021-09-07 2024-07-17 Wisconsin Alumni Research Foundation Cardiac fibroblast derived extracellular matrix
CN115197892A (en) * 2022-06-13 2022-10-18 浙江省人民医院 Separation culture method of fibroblast in human papillary thyroid carcinoma tissue

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573937A (en) * 1989-12-07 1996-11-12 Snow Brand Milk Products Co., Ltd. Serum free culture medium
CA2555021A1 (en) * 2004-02-13 2005-08-25 Reprocell, Inc. Medium for preparing feeder cells for embryonic stem cells and feeder cells
EP1851305B1 (en) * 2005-02-11 2012-01-18 Novo Nordisk Health Care AG Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate

Also Published As

Publication number Publication date
CN102471761A (en) 2012-05-23
WO2011009706A1 (en) 2011-01-27
IL217352A (en) 2016-03-31
AU2010275678B2 (en) 2016-06-16
AU2010275678A1 (en) 2012-02-09
ES2650664T3 (en) 2018-01-19
FR2948381B1 (en) 2013-07-12
EP2456854B1 (en) 2017-08-30
JP2013500003A (en) 2013-01-07
CA2767929A1 (en) 2011-01-27
MX2012001004A (en) 2012-05-29
EP2456854A1 (en) 2012-05-30
IL217352A0 (en) 2012-02-29
US9243228B2 (en) 2016-01-26
FR2948381A1 (en) 2011-01-28
US20120190109A1 (en) 2012-07-26
JP5758891B2 (en) 2015-08-05

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Legal Events

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PSEA Patent sealed
RENW Renewal (renewal fees accepted)

Free format text: PATENT RENEWED FOR 3 YEARS UNTIL 02 JUL 2017 BY CPA GLOBAL

Effective date: 20140620

RENW Renewal (renewal fees accepted)

Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 02 JUL 2018 BY DENNEMEYER + CO

Effective date: 20170627

LAPS Patent lapsed