MX2012001004A - Proceso para la obtencion de miofibroblastos. - Google Patents

Proceso para la obtencion de miofibroblastos.

Info

Publication number
MX2012001004A
MX2012001004A MX2012001004A MX2012001004A MX2012001004A MX 2012001004 A MX2012001004 A MX 2012001004A MX 2012001004 A MX2012001004 A MX 2012001004A MX 2012001004 A MX2012001004 A MX 2012001004A MX 2012001004 A MX2012001004 A MX 2012001004A
Authority
MX
Mexico
Prior art keywords
identification
myofibroblasts
cells
myofibroblast
population
Prior art date
Application number
MX2012001004A
Other languages
English (en)
Inventor
Nicolas Jacques Rouyer
Robert Barthel
Original Assignee
Oncobiotek
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncobiotek filed Critical Oncobiotek
Publication of MX2012001004A publication Critical patent/MX2012001004A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un proceso para la obtención de miofibroblastos. De conformidad con este proceso: (a) se prepara una muestra de células que comprende esencialmente fibroblastos; y (b) esta muestra de células se cultiva en un medio de cultivo libre de suero. El propósito principal atendido por la invención es obtener una población de miofibroblastos, las características de la cual facilitan cualquier estudio de estas células, y en particular tan pura como sea posible una población de miofibroblastos. Algunos ejemplos de aplicación de la invención son: identificación de biomarcadores de miofibroblastos, identificación de objetivos terapéuticos, identificación y validación de compuestos anticancerígenos, y un modelo in vitro para selección de compuestos farmacéuticos o cosméticos.
MX2012001004A 2009-07-24 2010-07-02 Proceso para la obtencion de miofibroblastos. MX2012001004A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0955216A FR2948381B1 (fr) 2009-07-24 2009-07-24 Procede d'obtention de myofibroblastes.
PCT/EP2010/059478 WO2011009706A1 (fr) 2009-07-24 2010-07-02 Procede d'obtention de myofibroblastes

Publications (1)

Publication Number Publication Date
MX2012001004A true MX2012001004A (es) 2012-05-29

Family

ID=41460947

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012001004A MX2012001004A (es) 2009-07-24 2010-07-02 Proceso para la obtencion de miofibroblastos.

Country Status (12)

Country Link
US (1) US9243228B2 (es)
EP (1) EP2456854B1 (es)
JP (1) JP5758891B2 (es)
CN (1) CN102471761A (es)
AU (1) AU2010275678B2 (es)
CA (1) CA2767929A1 (es)
ES (1) ES2650664T3 (es)
FR (1) FR2948381B1 (es)
IL (1) IL217352A (es)
MX (1) MX2012001004A (es)
NZ (1) NZ597728A (es)
WO (1) WO2011009706A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096265B (zh) * 2014-07-01 2016-03-23 温州医科大学 一种用于构建人工血管模型的三维收缩模型的制备方法
CN104630134A (zh) * 2015-02-15 2015-05-20 叶中华 阴道上皮细胞培养基及阴道上皮细胞培养方法
CN111500527A (zh) * 2020-06-30 2020-08-07 北京昱龙盛世生物科技有限公司 一种表皮干细胞的分离培养方法
IL311054A (en) * 2021-09-07 2024-04-01 Wisconsin Alumni Res Found Extracellular matrix derived from cardiac fibroblast
CN115197892A (zh) * 2022-06-13 2022-10-18 浙江省人民医院 一种人甲状腺乳头状癌组织中成纤维细胞的分离培养方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573937A (en) * 1989-12-07 1996-11-12 Snow Brand Milk Products Co., Ltd. Serum free culture medium
EP1715033A4 (en) * 2004-02-13 2008-06-11 Asahi Techno Glass Corp MEANS FOR PREPARING NOURISHED CELLS FOR EMBRYONIC STEM CELLS AND NOURISHED CELLS
EP1851305B1 (en) * 2005-02-11 2012-01-18 Novo Nordisk Health Care AG Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate

Also Published As

Publication number Publication date
WO2011009706A1 (fr) 2011-01-27
ES2650664T3 (es) 2018-01-19
FR2948381B1 (fr) 2013-07-12
IL217352A0 (en) 2012-02-29
NZ597728A (en) 2013-11-29
US20120190109A1 (en) 2012-07-26
CA2767929A1 (fr) 2011-01-27
CN102471761A (zh) 2012-05-23
JP5758891B2 (ja) 2015-08-05
EP2456854B1 (fr) 2017-08-30
AU2010275678A1 (en) 2012-02-09
JP2013500003A (ja) 2013-01-07
FR2948381A1 (fr) 2011-01-28
IL217352A (en) 2016-03-31
AU2010275678B2 (en) 2016-06-16
EP2456854A1 (fr) 2012-05-30
US9243228B2 (en) 2016-01-26

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