PT2573167T - Processo para a produção de emplastros ou curativos de pele autóloga através de cultivo de queratinócitos e fibroblastos autólogos com soro autólogo para a geração de pele - Google Patents

Processo para a produção de emplastros ou curativos de pele autóloga através de cultivo de queratinócitos e fibroblastos autólogos com soro autólogo para a geração de pele

Info

Publication number
PT2573167T
PT2573167T PT108516873T PT10851687T PT2573167T PT 2573167 T PT2573167 T PT 2573167T PT 108516873 T PT108516873 T PT 108516873T PT 10851687 T PT10851687 T PT 10851687T PT 2573167 T PT2573167 T PT 2573167T
Authority
PT
Portugal
Prior art keywords
autologous
skin
dressings
fibroblasts
patches
Prior art date
Application number
PT108516873T
Other languages
English (en)
Inventor
Carlos Zambrano Burgl Juan
Cristina Gaona Silva Jennifer
Original Assignee
Rodrigo Focion Soto Pareja
Carlos Zambrano Burgl Juan
Cristina Gaona Silva Jennifer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rodrigo Focion Soto Pareja, Carlos Zambrano Burgl Juan, Cristina Gaona Silva Jennifer filed Critical Rodrigo Focion Soto Pareja
Publication of PT2573167T publication Critical patent/PT2573167T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PT108516873T 2010-05-21 2010-05-21 Processo para a produção de emplastros ou curativos de pele autóloga através de cultivo de queratinócitos e fibroblastos autólogos com soro autólogo para a geração de pele PT2573167T (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10851687.3A EP2573167B1 (en) 2010-05-21 2010-05-21 Process for the production of patches or dressings of autologous skin through cultivation of autologous keratinocytes and fibroblasts with autologous serum for the generation of skin
PCT/IB2010/001215 WO2011144956A1 (es) 2010-05-21 2010-05-21 Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion de piel

Publications (1)

Publication Number Publication Date
PT2573167T true PT2573167T (pt) 2019-12-10

Family

ID=44991239

Family Applications (1)

Application Number Title Priority Date Filing Date
PT108516873T PT2573167T (pt) 2010-05-21 2010-05-21 Processo para a produção de emplastros ou curativos de pele autóloga através de cultivo de queratinócitos e fibroblastos autólogos com soro autólogo para a geração de pele

Country Status (9)

Country Link
US (1) US9222076B2 (pt)
EP (1) EP2573167B1 (pt)
CN (1) CN103003415B (pt)
BR (1) BR112012029546B8 (pt)
CL (1) CL2012003244A1 (pt)
ES (1) ES2761307T3 (pt)
MX (1) MX339001B (pt)
PT (1) PT2573167T (pt)
WO (1) WO2011144956A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201303485D0 (en) 2013-02-27 2013-04-10 Alcyomics Ltd Skin model
MX342233B (es) * 2014-02-04 2016-08-24 Alvaro Galue Eduardo Proceso de obtencion de un compuesto de aspersion celular de fibroblastos y queratinocitos humanos en solucion y su uso como agente regenerativo de lesiones cutáneas.
US20180258380A1 (en) * 2015-09-15 2018-09-13 Stellenbosch University A method of culturing cells
KR101908030B1 (ko) * 2016-06-17 2018-10-15 울산대학교 산학협력단 상처 또는 궤양치료를 위한 3차원 세포시트 및 이의 제조방법
CN106978387A (zh) * 2017-03-31 2017-07-25 申传安 一种改良的消化提取角质形成细胞的新方法
IT201800010390A1 (it) * 2018-11-16 2020-05-16 Diego Furlan Dispositivo medico monouso per la medicazione di lesioni della pelle di un paziente e relativi procedimenti di preparazione e utilizzazione
CN109456891B (zh) * 2018-12-05 2024-08-16 中国人民解放军第四军医大学 一种仿生组织工程表皮膜片高效血浆置换系统
WO2023192527A2 (en) * 2022-03-31 2023-10-05 Biolab Sciences, Inc. Skin graft composition and method of making and using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE35399E (en) * 1990-04-24 1996-12-10 Eisenberg; Mark Composite living skin equivalents
AU784221B2 (en) 1999-07-20 2006-02-23 Smith & Nephew Orthopaedics Ag Improved keratinocyte culture and uses thereof
CN1363398A (zh) * 2002-01-16 2002-08-14 吉林大学 以壳多糖或其衍生物为基质网架的复层人工皮肤
CN1193802C (zh) * 2002-07-15 2005-03-23 上海组织工程研究与开发中心 双层人工皮肤及其制备方法
CN1224429C (zh) * 2002-12-04 2005-10-26 曹谊林 人工皮肤移植物及其制备方法
HU227723B1 (en) * 2004-07-09 2012-01-30 Nagy Norbert Dr Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy
EP1937326B1 (en) * 2005-10-21 2018-09-12 CellResearch Corporation Pte Ltd Isolation and cultivation of stem/progenitor cells from the amniotic membrane of umbilical cord and uses of cells differentiated therefrom

Also Published As

Publication number Publication date
BR112012029546A2 (pt) 2015-10-13
EP2573167A4 (en) 2014-10-22
CL2012003244A1 (es) 2013-10-18
ES2761307T3 (es) 2020-05-19
US9222076B2 (en) 2015-12-29
EP2573167A1 (en) 2013-03-27
CN103003415A (zh) 2013-03-27
BR112012029546B8 (pt) 2021-07-27
US20130195958A1 (en) 2013-08-01
BR112012029546B1 (pt) 2021-04-06
MX2012013500A (es) 2013-06-05
MX339001B (es) 2016-05-09
CN103003415B (zh) 2017-02-08
EP2573167B1 (en) 2019-11-06
WO2011144956A1 (es) 2011-11-24

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