CL2012003244A1 - Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion; parche o apósito que comprende un sustrato de malla sobre la cual se ha colocado colágeno, encima queratinocitos y encima fibroblastos. - Google Patents

Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion; parche o apósito que comprende un sustrato de malla sobre la cual se ha colocado colágeno, encima queratinocitos y encima fibroblastos.

Info

Publication number
CL2012003244A1
CL2012003244A1 CL2012003244A CL2012003244A CL2012003244A1 CL 2012003244 A1 CL2012003244 A1 CL 2012003244A1 CL 2012003244 A CL2012003244 A CL 2012003244A CL 2012003244 A CL2012003244 A CL 2012003244A CL 2012003244 A1 CL2012003244 A1 CL 2012003244A1
Authority
CL
Chile
Prior art keywords
autologous
apositos
keratinocytes
fibroblasts
dressing
Prior art date
Application number
CL2012003244A
Other languages
English (en)
Inventor
Burgk Juan Carlos Zambrano
Silva Jennifer Cristina Gaona
Original Assignee
Soto Pareja Rodrigo Focion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Soto Pareja Rodrigo Focion filed Critical Soto Pareja Rodrigo Focion
Publication of CL2012003244A1 publication Critical patent/CL2012003244A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CL2012003244A 2010-05-21 2012-11-21 Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion; parche o apósito que comprende un sustrato de malla sobre la cual se ha colocado colágeno, encima queratinocitos y encima fibroblastos. CL2012003244A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2010/001215 WO2011144956A1 (es) 2010-05-21 2010-05-21 Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion de piel

Publications (1)

Publication Number Publication Date
CL2012003244A1 true CL2012003244A1 (es) 2013-10-18

Family

ID=44991239

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2012003244A CL2012003244A1 (es) 2010-05-21 2012-11-21 Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion; parche o apósito que comprende un sustrato de malla sobre la cual se ha colocado colágeno, encima queratinocitos y encima fibroblastos.

Country Status (9)

Country Link
US (1) US9222076B2 (es)
EP (1) EP2573167B1 (es)
CN (1) CN103003415B (es)
BR (1) BR112012029546B8 (es)
CL (1) CL2012003244A1 (es)
ES (1) ES2761307T3 (es)
MX (1) MX339001B (es)
PT (1) PT2573167T (es)
WO (1) WO2011144956A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201303485D0 (en) 2013-02-27 2013-04-10 Alcyomics Ltd Skin model
MX342233B (es) * 2014-02-04 2016-08-24 Alvaro Galue Eduardo Proceso de obtencion de un compuesto de aspersion celular de fibroblastos y queratinocitos humanos en solucion y su uso como agente regenerativo de lesiones cutáneas.
CN108350420A (zh) * 2015-09-15 2018-07-31 斯坦陵布什大学 培养细胞的方法
KR101908030B1 (ko) 2016-06-17 2018-10-15 울산대학교 산학협력단 상처 또는 궤양치료를 위한 3차원 세포시트 및 이의 제조방법
CN106978387A (zh) * 2017-03-31 2017-07-25 申传安 一种改良的消化提取角质形成细胞的新方法
IT201800010390A1 (it) * 2018-11-16 2020-05-16 Diego Furlan Dispositivo medico monouso per la medicazione di lesioni della pelle di un paziente e relativi procedimenti di preparazione e utilizzazione
CN109456891A (zh) * 2018-12-05 2019-03-12 中国人民解放军第四军医大学 一种仿生组织工程表皮膜片高效血浆置换系统
WO2023192527A2 (en) * 2022-03-31 2023-10-05 Biolab Sciences, Inc. Skin graft composition and method of making and using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5282859A (en) * 1990-04-24 1994-02-01 Mark Eisenberg Composite living skin equivalents
JP3738273B2 (ja) 1999-07-20 2006-01-25 ディーエフビー ファーマシューティカルズ， インコーポレイテッド 改良ケラチノサイト培養物およびその使用
CN1363398A (zh) * 2002-01-16 2002-08-14 吉林大学 以壳多糖或其衍生物为基质网架的复层人工皮肤
CN1193802C (zh) * 2002-07-15 2005-03-23 上海组织工程研究与开发中心 双层人工皮肤及其制备方法
CN1224429C (zh) * 2002-12-04 2005-10-26 曹谊林 人工皮肤移植物及其制备方法
HU227723B1 (en) * 2004-07-09 2012-01-30 Nagy Norbert Dr Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy
CN101330935B (zh) * 2005-10-21 2013-11-13 细胞研究私人有限公司 自脐带羊膜分离和培养干/祖细胞及其分化的细胞的应用

Also Published As

Publication number Publication date
EP2573167A1 (en) 2013-03-27
US9222076B2 (en) 2015-12-29
WO2011144956A1 (es) 2011-11-24
EP2573167B1 (en) 2019-11-06
ES2761307T3 (es) 2020-05-19
PT2573167T (pt) 2019-12-10
US20130195958A1 (en) 2013-08-01
MX339001B (es) 2016-05-09
EP2573167A4 (en) 2014-10-22
BR112012029546A2 (pt) 2015-10-13
BR112012029546B1 (pt) 2021-04-06
BR112012029546B8 (pt) 2021-07-27
CN103003415A (zh) 2013-03-27
MX2012013500A (es) 2013-06-05
CN103003415B (zh) 2017-02-08

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