MX339001B - Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion de piel. - Google Patents

Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion de piel.

Info

Publication number
MX339001B
MX339001B MX2012013500A MX2012013500A MX339001B MX 339001 B MX339001 B MX 339001B MX 2012013500 A MX2012013500 A MX 2012013500A MX 2012013500 A MX2012013500 A MX 2012013500A MX 339001 B MX339001 B MX 339001B
Authority
MX
Mexico
Prior art keywords
autologous
skin
dressings
fibroblasts
patches
Prior art date
Application number
MX2012013500A
Other languages
English (en)
Other versions
MX2012013500A (es
Inventor
Juan Carlos Zambrano Burgl
Jennifer Cristina Gaona Silva
Original Assignee
Rodrigo Foción Soto Pareja
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rodrigo Foción Soto Pareja filed Critical Rodrigo Foción Soto Pareja
Publication of MX2012013500A publication Critical patent/MX2012013500A/es
Publication of MX339001B publication Critical patent/MX339001B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a el proceso en general se basa en la toma de una muestra de piel y una muestra de sangre del paciente y en base a estos dos elementos se cultiva la piel, la cual es colocada sobre un parche de colágeno para producir un apósito que posteriormente se coloca sobre un paciente que lo requiera.
MX2012013500A 2010-05-21 2010-05-21 Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion de piel. MX339001B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2010/001215 WO2011144956A1 (es) 2010-05-21 2010-05-21 Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion de piel

Publications (2)

Publication Number Publication Date
MX2012013500A MX2012013500A (es) 2013-06-05
MX339001B true MX339001B (es) 2016-05-09

Family

ID=44991239

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012013500A MX339001B (es) 2010-05-21 2010-05-21 Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion de piel.

Country Status (9)

Country Link
US (1) US9222076B2 (es)
EP (1) EP2573167B1 (es)
CN (1) CN103003415B (es)
BR (1) BR112012029546B8 (es)
CL (1) CL2012003244A1 (es)
ES (1) ES2761307T3 (es)
MX (1) MX339001B (es)
PT (1) PT2573167T (es)
WO (1) WO2011144956A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201303485D0 (en) 2013-02-27 2013-04-10 Alcyomics Ltd Skin model
MX342233B (es) * 2014-02-04 2016-08-24 Alvaro Galue Eduardo Proceso de obtencion de un compuesto de aspersion celular de fibroblastos y queratinocitos humanos en solucion y su uso como agente regenerativo de lesiones cutáneas.
MX2018003141A (es) * 2015-09-15 2019-02-07 Univ Stellenbosch Metodo para cultivar celulas.
KR101908030B1 (ko) * 2016-06-17 2018-10-15 울산대학교 산학협력단 상처 또는 궤양치료를 위한 3차원 세포시트 및 이의 제조방법
CN106978387A (zh) * 2017-03-31 2017-07-25 申传安 一种改良的消化提取角质形成细胞的新方法
IT201800010390A1 (it) * 2018-11-16 2020-05-16 Diego Furlan Dispositivo medico monouso per la medicazione di lesioni della pelle di un paziente e relativi procedimenti di preparazione e utilizzazione
CN109456891B (zh) * 2018-12-05 2024-08-16 中国人民解放军第四军医大学 一种仿生组织工程表皮膜片高效血浆置换系统
WO2023192527A2 (en) * 2022-03-31 2023-10-05 Biolab Sciences, Inc. Skin graft composition and method of making and using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5282859A (en) * 1990-04-24 1994-02-01 Mark Eisenberg Composite living skin equivalents
EP1702979B1 (en) * 1999-07-20 2012-05-30 DFB Technology Holdings, LLC Improved keratinocyte culture and uses thereof
CN1363398A (zh) * 2002-01-16 2002-08-14 吉林大学 以壳多糖或其衍生物为基质网架的复层人工皮肤
CN1193802C (zh) * 2002-07-15 2005-03-23 上海组织工程研究与开发中心 双层人工皮肤及其制备方法
CN1224429C (zh) * 2002-12-04 2005-10-26 曹谊林 人工皮肤移植物及其制备方法
HU227723B1 (en) * 2004-07-09 2012-01-30 Nagy Norbert Dr Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy
US8287854B2 (en) * 2005-10-21 2012-10-16 Cellresearch Corporation Pte Ltd Skin equivalents derived from umbilical cord mesenchymal stem/progenitor cells and umbilical cord epithelial stem/progenitor cells

Also Published As

Publication number Publication date
MX2012013500A (es) 2013-06-05
EP2573167A1 (en) 2013-03-27
BR112012029546B1 (pt) 2021-04-06
EP2573167A4 (en) 2014-10-22
US9222076B2 (en) 2015-12-29
PT2573167T (pt) 2019-12-10
ES2761307T3 (es) 2020-05-19
CL2012003244A1 (es) 2013-10-18
BR112012029546B8 (pt) 2021-07-27
BR112012029546A2 (pt) 2015-10-13
CN103003415B (zh) 2017-02-08
US20130195958A1 (en) 2013-08-01
EP2573167B1 (en) 2019-11-06
WO2011144956A1 (es) 2011-11-24
CN103003415A (zh) 2013-03-27

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