BR112012021267A2 - métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes. - Google Patents
métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes.Info
- Publication number
- BR112012021267A2 BR112012021267A2 BR112012021267A BR112012021267A BR112012021267A2 BR 112012021267 A2 BR112012021267 A2 BR 112012021267A2 BR 112012021267 A BR112012021267 A BR 112012021267A BR 112012021267 A BR112012021267 A BR 112012021267A BR 112012021267 A2 BR112012021267 A2 BR 112012021267A2
- Authority
- BR
- Brazil
- Prior art keywords
- stem cells
- melanocytes
- human
- preparing
- methods
- Prior art date
Links
- 210000002752 melanocyte Anatomy 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 210000000130 stem cell Anatomy 0.000 title abstract 2
- 210000001778 pluripotent stem cell Anatomy 0.000 abstract 2
- 239000002269 analeptic agent Substances 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000011712 cell development Effects 0.000 abstract 1
- 238000002659 cell therapy Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 210000002510 keratinocyte Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0626—Melanocytes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/40—Nucleotides, nucleosides or bases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/395—Thyroid hormones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Abstract
métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes. a invenção refere-se a um método ex vivo para obtenção de uma população de melanócitos humanos derivados de células tronco pluripotentes humanas compreendendo a etapa que consiste no cocultivo de células tronco pluripotentes humanas com células que suportam a diferenciação ectodérmica na presença de um agente que estimula a indução epidérmica e de um agente que estimula a diferenciação terminal de queratinócitos. a invenção também se refere aos melanócitos humanos obteníveis pelo dito método e seus usos em terapia celular e em ensaios de varredura.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US30705610P | 2010-02-23 | 2010-02-23 | |
EP10305183 | 2010-02-24 | ||
PCT/EP2011/052504 WO2011104200A1 (en) | 2010-02-23 | 2011-02-21 | Methods for preparing human melanocytes from human pluripotent stem cells |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112012021267A2 true BR112012021267A2 (pt) | 2015-09-22 |
Family
ID=42562566
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112012021267A BR112012021267A2 (pt) | 2010-02-23 | 2011-02-21 | métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes. |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP2539438A1 (pt) |
JP (1) | JP2013520163A (pt) |
KR (1) | KR20120120971A (pt) |
CN (1) | CN102858955A (pt) |
AU (1) | AU2011219882A1 (pt) |
BR (1) | BR112012021267A2 (pt) |
SG (1) | SG183097A1 (pt) |
WO (1) | WO2011104200A1 (pt) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5902442B2 (ja) * | 2011-11-16 | 2016-04-13 | 日本メナード化粧品株式会社 | メラノサイト分化誘導促進剤及びその使用方法 |
JP6101066B2 (ja) * | 2012-12-10 | 2017-03-22 | 日本メナード化粧品株式会社 | メラノサイト集団の調製方法 |
JP2016516394A (ja) * | 2013-04-02 | 2016-06-09 | 株式会社Clio | ヒト線維芽細胞において特徴的な幹細胞であるMuse細胞から容易に分化した機能性メラノサイトの使用 |
ITMI20131053A1 (it) * | 2013-06-25 | 2014-12-26 | Maria Oliva Salviati | Composizione che comprende ortica |
CN104651298B (zh) * | 2013-11-21 | 2019-06-04 | 上海交通大学医学院附属第九人民医院 | 诱导胚胎干细胞定向分化为角化细胞的培养基及方法 |
JP2015146803A (ja) * | 2014-01-10 | 2015-08-20 | 学校法人 聖マリアンナ医科大学 | メラノサイトの分化誘導方法 |
CN104789521B (zh) * | 2014-01-22 | 2017-11-07 | 广州康睿生物医药科技股份有限公司 | 一种晶状体上皮干细胞的分离培养方法 |
US11022600B2 (en) * | 2014-05-01 | 2021-06-01 | Shimadzu Corporation | Method for assessing state of differentiation of cells |
US10844352B2 (en) | 2014-11-18 | 2020-11-24 | The Trustees Of The University of Pennsyivania | Compositions and methods for the generation of melanocytes through direct reprogramming |
CN105950543A (zh) * | 2016-07-19 | 2016-09-21 | 安徽惠恩生物科技股份有限公司 | 一种表皮细胞的扩增制备方法 |
JP2017035105A (ja) * | 2016-10-24 | 2017-02-16 | 日本メナード化粧品株式会社 | メラノサイト分化誘導抑制剤及びその使用方法 |
JP7080647B2 (ja) * | 2017-01-18 | 2022-06-06 | 民裕 川上 | 高分化度の多能性幹細胞由来細胞の製造方法 |
PL236298B1 (pl) * | 2017-09-21 | 2020-12-28 | Univ Jagiellonski | Sposób otrzymywania upigmentowanych komórek in vitro poprzez różnicowanie ludzkich indukowanych pluripotencjalnych komórek macierzystych |
CN108154503A (zh) * | 2017-12-13 | 2018-06-12 | 西安交通大学医学院第附属医院 | 一种基于图像处理的白癜风病情诊断系统 |
CN108373990B (zh) * | 2018-03-19 | 2021-08-24 | 沈阳艾米奥生物工程技术研发中心有限公司 | 人羊膜上皮干细胞向黑色素细胞诱导分化培养基及其方法 |
EP4397751A1 (en) * | 2023-01-04 | 2024-07-10 | Centre d'Etude des Cellules Souches (CECS) | Methods for the production of human melanocytes from pluripotent stem cells |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR9106354A (pt) | 1990-04-24 | 1993-04-27 | Mark Eisenberg | Equivalente de pele viva composta,seu processo de preparacao; kit de teste,seu processo de preparacao,e processo de testar efeito de substancia sobre a pele |
IT1248934B (it) | 1990-06-01 | 1995-02-11 | Fidia Spa | Membrane forate biocompatibili,processi per la loro preparazione,loro impiego come supporto per la crescita in vitro di cellule epiteliali, pelli artificiali cosi' ottenute e loro impiego nei trapianti di pelle |
US5693332C1 (en) | 1995-08-11 | 2001-01-09 | Univ California | Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure |
HU227723B1 (en) * | 2004-07-09 | 2012-01-30 | Nagy Norbert Dr | Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy |
-
2011
- 2011-02-21 EP EP11704078A patent/EP2539438A1/en not_active Withdrawn
- 2011-02-21 BR BR112012021267A patent/BR112012021267A2/pt not_active IP Right Cessation
- 2011-02-21 JP JP2012553338A patent/JP2013520163A/ja not_active Withdrawn
- 2011-02-21 WO PCT/EP2011/052504 patent/WO2011104200A1/en active Application Filing
- 2011-02-21 KR KR1020127024841A patent/KR20120120971A/ko not_active Application Discontinuation
- 2011-02-21 AU AU2011219882A patent/AU2011219882A1/en not_active Abandoned
- 2011-02-21 CN CN201180008443XA patent/CN102858955A/zh active Pending
- 2011-02-21 SG SG2012048922A patent/SG183097A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP2013520163A (ja) | 2013-06-06 |
WO2011104200A1 (en) | 2011-09-01 |
SG183097A1 (en) | 2012-09-27 |
AU2011219882A1 (en) | 2012-07-12 |
CN102858955A (zh) | 2013-01-02 |
EP2539438A1 (en) | 2013-01-02 |
KR20120120971A (ko) | 2012-11-02 |
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B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE AS 3A E 4A ANUIDADES. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2344 DE 08-12-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |