BR112012021267A2 - métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes. - Google Patents

métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes.

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Publication number
BR112012021267A2
BR112012021267A2 BR112012021267A BR112012021267A BR112012021267A2 BR 112012021267 A2 BR112012021267 A2 BR 112012021267A2 BR 112012021267 A BR112012021267 A BR 112012021267A BR 112012021267 A BR112012021267 A BR 112012021267A BR 112012021267 A2 BR112012021267 A2 BR 112012021267A2
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BR
Brazil
Prior art keywords
stem cells
melanocytes
human
preparing
methods
Prior art date
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BR112012021267A
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English (en)
Inventor
Christine Baldeschi
Gilles Lemaitre
Mare Peschanski
Xavier Nissan
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Inst Nat Sante Rech Med
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Publication date
Application filed by Inst Nat Sante Rech Med filed Critical Inst Nat Sante Rech Med
Publication of BR112012021267A2 publication Critical patent/BR112012021267A2/pt

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0626—Melanocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/395—Thyroid hormones
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes. a invenção refere-se a um método ex vivo para obtenção de uma população de melanócitos humanos derivados de células tronco pluripotentes humanas compreendendo a etapa que consiste no cocultivo de células tronco pluripotentes humanas com células que suportam a diferenciação ectodérmica na presença de um agente que estimula a indução epidérmica e de um agente que estimula a diferenciação terminal de queratinócitos. a invenção também se refere aos melanócitos humanos obteníveis pelo dito método e seus usos em terapia celular e em ensaios de varredura.
BR112012021267A 2010-02-23 2011-02-21 métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes. BR112012021267A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30705610P 2010-02-23 2010-02-23
EP10305183 2010-02-24
PCT/EP2011/052504 WO2011104200A1 (en) 2010-02-23 2011-02-21 Methods for preparing human melanocytes from human pluripotent stem cells

Publications (1)

Publication Number Publication Date
BR112012021267A2 true BR112012021267A2 (pt) 2015-09-22

Family

ID=42562566

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012021267A BR112012021267A2 (pt) 2010-02-23 2011-02-21 métodos para a preparação de melanócitos a partir de células tronco humanas pluripotentes.

Country Status (8)

Country Link
EP (1) EP2539438A1 (pt)
JP (1) JP2013520163A (pt)
KR (1) KR20120120971A (pt)
CN (1) CN102858955A (pt)
AU (1) AU2011219882A1 (pt)
BR (1) BR112012021267A2 (pt)
SG (1) SG183097A1 (pt)
WO (1) WO2011104200A1 (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5902442B2 (ja) * 2011-11-16 2016-04-13 日本メナード化粧品株式会社 メラノサイト分化誘導促進剤及びその使用方法
JP6101066B2 (ja) * 2012-12-10 2017-03-22 日本メナード化粧品株式会社 メラノサイト集団の調製方法
JP2016516394A (ja) * 2013-04-02 2016-06-09 株式会社Ｃｌｉｏ ヒト線維芽細胞において特徴的な幹細胞であるＭｕｓｅ細胞から容易に分化した機能性メラノサイトの使用
ITMI20131053A1 (it) * 2013-06-25 2014-12-26 Maria Oliva Salviati Composizione che comprende ortica
CN104651298B (zh) * 2013-11-21 2019-06-04 上海交通大学医学院附属第九人民医院 诱导胚胎干细胞定向分化为角化细胞的培养基及方法
JP2015146803A (ja) * 2014-01-10 2015-08-20 学校法人 聖マリアンナ医科大学 メラノサイトの分化誘導方法
CN104789521B (zh) * 2014-01-22 2017-11-07 广州康睿生物医药科技股份有限公司 一种晶状体上皮干细胞的分离培养方法
US11022600B2 (en) * 2014-05-01 2021-06-01 Shimadzu Corporation Method for assessing state of differentiation of cells
US10844352B2 (en) 2014-11-18 2020-11-24 The Trustees Of The University of Pennsyivania Compositions and methods for the generation of melanocytes through direct reprogramming
CN105950543A (zh) * 2016-07-19 2016-09-21 安徽惠恩生物科技股份有限公司 一种表皮细胞的扩增制备方法
JP2017035105A (ja) * 2016-10-24 2017-02-16 日本メナード化粧品株式会社 メラノサイト分化誘導抑制剤及びその使用方法
JP7080647B2 (ja) * 2017-01-18 2022-06-06 民裕 川上 高分化度の多能性幹細胞由来細胞の製造方法
PL236298B1 (pl) * 2017-09-21 2020-12-28 Univ Jagiellonski Sposób otrzymywania upigmentowanych komórek in vitro poprzez różnicowanie ludzkich indukowanych pluripotencjalnych komórek macierzystych
CN108154503A (zh) * 2017-12-13 2018-06-12 西安交通大学医学院第附属医院 一种基于图像处理的白癜风病情诊断系统
CN108373990B (zh) * 2018-03-19 2021-08-24 沈阳艾米奥生物工程技术研发中心有限公司 人羊膜上皮干细胞向黑色素细胞诱导分化培养基及其方法
EP4397751A1 (en) * 2023-01-04 2024-07-10 Centre d'Etude des Cellules Souches (CECS) Methods for the production of human melanocytes from pluripotent stem cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
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BR9106354A (pt) 1990-04-24 1993-04-27 Mark Eisenberg Equivalente de pele viva composta,seu processo de preparacao; kit de teste,seu processo de preparacao,e processo de testar efeito de substancia sobre a pele
IT1248934B (it) 1990-06-01 1995-02-11 Fidia Spa Membrane forate biocompatibili,processi per la loro preparazione,loro impiego come supporto per la crescita in vitro di cellule epiteliali, pelli artificiali cosi' ottenute e loro impiego nei trapianti di pelle
US5693332C1 (en) 1995-08-11 2001-01-09 Univ California Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure
HU227723B1 (en) * 2004-07-09 2012-01-30 Nagy Norbert Dr Autologous keratinocytes, melanocytes and fibroblast culturing technique and serum-free medium for use in human therapy

Also Published As

Publication number Publication date
JP2013520163A (ja) 2013-06-06
WO2011104200A1 (en) 2011-09-01
SG183097A1 (en) 2012-09-27
AU2011219882A1 (en) 2012-07-12
CN102858955A (zh) 2013-01-02
EP2539438A1 (en) 2013-01-02
KR20120120971A (ko) 2012-11-02

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2344 DE 08-12-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.