WO2011099783A3 - 지방조직 유래 줄기세포 및 단핵구로부터 세포성장인자의 생산방법 및 그 이용 - Google Patents

지방조직 유래 줄기세포 및 단핵구로부터 세포성장인자의 생산방법 및 그 이용 Download PDF

Info

Publication number
WO2011099783A3
WO2011099783A3 PCT/KR2011/000885 KR2011000885W WO2011099783A3 WO 2011099783 A3 WO2011099783 A3 WO 2011099783A3 KR 2011000885 W KR2011000885 W KR 2011000885W WO 2011099783 A3 WO2011099783 A3 WO 2011099783A3
Authority
WO
WIPO (PCT)
Prior art keywords
adipose
stem cells
derived stem
monocytes
culture
Prior art date
Application number
PCT/KR2011/000885
Other languages
English (en)
French (fr)
Other versions
WO2011099783A2 (ko
WO2011099783A9 (ko
Inventor
정영춘
홍영실
Original Assignee
허쉬바이오주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 허쉬바이오주식회사 filed Critical 허쉬바이오주식회사
Publication of WO2011099783A2 publication Critical patent/WO2011099783A2/ko
Publication of WO2011099783A9 publication Critical patent/WO2011099783A9/ko
Publication of WO2011099783A3 publication Critical patent/WO2011099783A3/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1157—Monocytes, macrophages

Abstract

본 발명은 지방조직 유래 줄기세포 및 단핵구로부터 세포성장인자에 관한 것으로, 보다 상세하게는, 본 발명은 (a) 지방조직 유래의 세포분획(Stromal Vascular Fraction, SVF)으로부터 지방유래 줄기세포를 배양하는 단계; (b) 상기 지방유래 줄기세포에 단핵구를 혼합하여 무혈청 배지에서 배양하는 단계; 및 (c) 상기 세포 배양액에서 세포 및 부유물을 배양물만을 수득하는 단계를 포함하는 지방조직 유래 줄기세포의 배양물을 제조하는 방법에 관한 것이다. 본 발명에 따른 배양물은 지방조직 유래 줄기세포를 단독으로 배양에 의한 것보다, 지방조직 유래 줄기세포 및 단핵구의 혼합배양으로부터 고농도의 성장인자가 함유된 배양물을 수득할 수 있기 때문에, 상기 세포에 의한 조직재생 등과 동일한 효과를 얻을 수 있고, 지방조직 유래 줄기세포를 신체에 직접 투여함으로써 야기될 수 있는 종양발생 관련 부작용을 방지할 수 있으므로, 이를 이용한 조직재생 및 피부개선 조성물의 제조에 유용하다.
PCT/KR2011/000885 2010-02-10 2011-02-10 지방조직 유래 줄기세포 및 단핵구로부터 세포성장인자의 생산방법 및 그 이용 WO2011099783A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0012310 2010-02-10
KR1020100012310A KR100995133B1 (ko) 2010-02-10 2010-02-10 지방조직 유래 줄기세포 및 단핵구로부터 세포성장인자의 생산방법 및 그 이용

Publications (3)

Publication Number Publication Date
WO2011099783A2 WO2011099783A2 (ko) 2011-08-18
WO2011099783A9 WO2011099783A9 (ko) 2011-11-17
WO2011099783A3 true WO2011099783A3 (ko) 2012-01-12

Family

ID=43409859

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/000885 WO2011099783A2 (ko) 2010-02-10 2011-02-10 지방조직 유래 줄기세포 및 단핵구로부터 세포성장인자의 생산방법 및 그 이용

Country Status (2)

Country Link
KR (1) KR100995133B1 (ko)
WO (1) WO2011099783A2 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2865652C (en) 2011-03-15 2020-12-29 Cell Ideas Pty Ltd Pharmaceutical compositions and topical use thereof
KR101970634B1 (ko) * 2011-06-02 2019-04-19 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 생체외 조직 배양 시스템을 위한 방법 및 용도
KR101518651B1 (ko) * 2013-12-31 2015-05-07 동국대학교 산학협력단 골 및 신생혈관 형성능이 향상된 줄기세포 공배양 방법
EP3148556A4 (en) * 2014-04-21 2017-11-08 Sanjay Dhar Skin treatment formulations
CN105267243B (zh) * 2014-07-23 2020-03-20 深圳培元生物科技有限公司 一种消除皮肤妊娠纹的干细胞提取物
KR101656511B1 (ko) * 2014-11-21 2016-09-12 휴젤(주) 육모 및 탈모방지능이 개선된 지방줄기세포 배양액 및 그의 제조방법
KR102091442B1 (ko) 2016-07-18 2020-03-20 (주)안트로젠 건 또는 인대 손상 치유를 위한 자가 및 동종의 지방유래 중간엽줄기세포 조성물 및 이의 제조방법
CN116410921B (zh) * 2023-02-09 2024-01-23 北京益华生物科技有限公司 一种人源脐带间充质干细胞诱导培养基、诱导方法及应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060147430A1 (en) * 2004-12-30 2006-07-06 Primegen Biotech Llc Adipose-derived stem cells for tissue regeneration and wound healing
KR20080097593A (ko) * 2007-05-02 2008-11-06 라정찬 인간 지방조직 유래 다분화능 줄기세포 및 모낭세포를함유하는 세포치료제
KR20080109725A (ko) * 2006-01-27 2008-12-17 (주)프로스테믹스 지방유래 줄기세포를 이용한 성장인자의 대량 생산방법
JP2009001509A (ja) * 2007-06-19 2009-01-08 Univ Nagoya 脂肪組織由来幹細胞を用いた組織再生用組成物
KR100920951B1 (ko) * 2007-08-01 2009-10-09 한양대학교 산학협력단 미분화 인간 지방조직 유래 줄기세포를 포함하는 골 재생용복합 지지체
KR20100008763A (ko) * 2008-07-16 2010-01-26 주식회사 알앤엘바이오 지방조직 유래 다분화능 줄기세포의 배양물 및 그 추출 단백질을 함유한 화장료용 조성물

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2642664A1 (en) 2006-02-16 2007-08-30 Burnham Institute For Medical Research Media conditioned by human embryonic stem cells or other progenitor cells and uses therefor

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060147430A1 (en) * 2004-12-30 2006-07-06 Primegen Biotech Llc Adipose-derived stem cells for tissue regeneration and wound healing
KR20080109725A (ko) * 2006-01-27 2008-12-17 (주)프로스테믹스 지방유래 줄기세포를 이용한 성장인자의 대량 생산방법
KR20080097593A (ko) * 2007-05-02 2008-11-06 라정찬 인간 지방조직 유래 다분화능 줄기세포 및 모낭세포를함유하는 세포치료제
JP2009001509A (ja) * 2007-06-19 2009-01-08 Univ Nagoya 脂肪組織由来幹細胞を用いた組織再生用組成物
KR100920951B1 (ko) * 2007-08-01 2009-10-09 한양대학교 산학협력단 미분화 인간 지방조직 유래 줄기세포를 포함하는 골 재생용복합 지지체
KR20100008763A (ko) * 2008-07-16 2010-01-26 주식회사 알앤엘바이오 지방조직 유래 다분화능 줄기세포의 배양물 및 그 추출 단백질을 함유한 화장료용 조성물

Also Published As

Publication number Publication date
WO2011099783A2 (ko) 2011-08-18
WO2011099783A9 (ko) 2011-11-17
KR100995133B1 (ko) 2010-11-18

Similar Documents

Publication Publication Date Title
WO2011099783A3 (ko) 지방조직 유래 줄기세포 및 단핵구로부터 세포성장인자의 생산방법 및 그 이용
WO2011091350A3 (en) Methods & compositions for improving protein production
WO2010096588A3 (en) Cell culture media containing combinations of proteins
WO2010141801A3 (en) Reprogramming t cells and hematophietic cells
WO2012135621A3 (en) Priming of pluripotent stem cells for neural differentiation
SG178914A1 (en) Stem cell conditioned medium compositions
MY156588A (en) Systems using cell culture for production of isoprene
WO2012021845A3 (en) Improved hematopoietic stem and progenitor cell therapy
MY181513A (en) Cell culture compositions and methods for polypeptide production
EP2633036A4 (en) METHODS OF DIFFERENTIATION FOR THE PRODUCTION OF GLIAL CELL POPULATIONS
WO2011075686A3 (en) Methods & compositions comprising heat shock proteins
WO2012056109A3 (en) Plant derived cell culture material
WO2011149762A3 (en) Method of nociceptor differentiation of human embryonic stem cells and uses thereof
EP2588594A4 (en) METHODS FOR CULTURING INDIFFERENCED CELLS USING EXTENDED RELEASE COMPOSITIONS
WO2012142569A3 (en) Decellularized extracellular matrix
WO2009145419A9 (ko) 식물성 펩톤을 포함하는 줄기세포 증식 촉진용 조성물
WO2011150055A3 (en) Methods, substrates, and systems useful for cell seeding of medical grafts
WO2012158244A3 (en) Thermostable variants of fibroblast growth factors
WO2012048010A3 (en) Compositions of adult organ stem cells and uses thereof
WO2014093505A3 (en) Acp-mediated production of fatty acid derivatives
MX2014006570A (es) Cocteles de enzimas preparados a partir de cultivos mezclados.
WO2012075462A3 (en) Methods and compositions for treatment of muscular dystrophy
WO2010092571A3 (en) Short beta-defensin-derived peptides
WO2010108058A3 (en) Small molecule inhibitors of dusp6 and uses therefor
EP3666900A3 (en) Cell-based production of nonulosonates

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11742475

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11742475

Country of ref document: EP

Kind code of ref document: A2