WO2011037416A3 - 세포이식술을 위한 혼합세포복합체인 세포스페로이드의 제조방법 및 이의 이용방법 - Google Patents

세포이식술을 위한 혼합세포복합체인 세포스페로이드의 제조방법 및 이의 이용방법 Download PDF

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WO2011037416A3
WO2011037416A3 PCT/KR2010/006512 KR2010006512W WO2011037416A3 WO 2011037416 A3 WO2011037416 A3 WO 2011037416A3 KR 2010006512 W KR2010006512 W KR 2010006512W WO 2011037416 A3 WO2011037416 A3 WO 2011037416A3
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cell
cells
spheroids
transplantation therapy
usage
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PCT/KR2010/006512
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French (fr)
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WO2011037416A9 (ko
WO2011037416A2 (ko
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이정익
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Lee Jeong Ik
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Publication of WO2011037416A9 publication Critical patent/WO2011037416A9/ko
Publication of WO2011037416A3 publication Critical patent/WO2011037416A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Cell Biology (AREA)
  • Biotechnology (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
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  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Dermatology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

본 발명자는 임상에 효과적이면서 실질적으로 이용 가능한 이식치료용 세포스페로이드를 제공함으로, 정상에서 벗어난 질병상태에 있는, 일부 혹은 전부의 손상 또는 결손된 환부에 이식치료하기 위한 것으로서, 신체내 정상적 혹은 건강한 상태와 유사한 조직으로서 형질발현된 신체내를 구성하는 각종의 조직을 대신 할 수 있는 본 발명를 이용한 각 질환에 대한 치료법 그리고, 피검물질의 약효 및 독성을 평가하기 위한 시스템으로서 상당히 유용한 것이라는 것을 확인하고 본 발명을 완성하기에 이르렀다. 본 발명의 한 관점에 의하면, (1) 혼합세포복합체를 구성하는 각세포재료를 분리준비하는 단계；(2) 상기분리 된세포를 계대배양하여 증폭하는 단계；(3) 1종류 혹은 2종류이상의 세포를 배양액내에서 단독 혹은 혼합한 세포재료혼탁액을 준비하고 세포를 고밀도의 부유상태로 유지시키는 배양을 함으로써 세포재료들을 상호접착시켜 혼합세포복합체를 제조하는 단계；및 (4) 세포스페로이드를 분리하여 각각의 목적별 사용방법에 따른 소정의 장소로 이동시키는 단계를 포함한 이식세포치료용 스페로이드의 제조방법 및 이용방법을 제공한다.
PCT/KR2010/006512 2009-09-23 2010-09-21 세포이식술을 위한 혼합세포복합체인 세포스페로이드의 제조방법 및 이의 이용방법 WO2011037416A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090089912A KR101649375B1 (ko) 2009-09-23 2009-09-23 세포이식술을 위한 혼합세포복합체인 세포스페로이드의 제조방법 및 이의 이용방법
KR10-2009-0089912 2009-09-23

Publications (3)

Publication Number Publication Date
WO2011037416A2 WO2011037416A2 (ko) 2011-03-31
WO2011037416A9 WO2011037416A9 (ko) 2011-09-01
WO2011037416A3 true WO2011037416A3 (ko) 2011-10-27

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PCT/KR2010/006512 WO2011037416A2 (ko) 2009-09-23 2010-09-21 세포이식술을 위한 혼합세포복합체인 세포스페로이드의 제조방법 및 이의 이용방법

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KR (1) KR101649375B1 (ko)
WO (1) WO2011037416A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101637937B1 (ko) * 2014-05-14 2016-07-08 서울대학교산학협력단 줄기세포 및 췌장소도 세포를 이용한 당뇨병 치료용 헤테로 스페로이드 제조 방법 및 이의 용도
AU2018290628B2 (en) * 2017-06-30 2022-03-17 Kolon Life Science, Inc. Pharmaceutical composition for preventing or treating osteoarthritis
WO2020197337A1 (ko) * 2019-03-28 2020-10-01 아주대학교산학협력단 조직 접착 및 분화 특성을 갖는 조직 유합용 조성물 및 이의 제조방법

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AHMED, N. ET AL.: "Soluble signalling factors derived from differentiated cartilage tissue affect chondrogenic differentiation of rat adult marrow stromal cells", CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, vol. 20, 2007, pages 665 - 678 *
BYUN, S. ET AL.: "Augmentation cytoplasty using hydorxapatite/ chitosan composite sheet seeded with autologous muscle-derived stem cells", KOREAN JOURNAL OF UROLOGY, vol. 48, no. 4, 30 April 2007 (2007-04-30), pages 433 - 438 *
LEE, H. N. ET AL.: "Muscle derived stem cell/ alginate/ polycaprolactone/ injection therapy in rats with denervated urethral sphincter", KOREAN JOURNAL OF UROLOGY, vol. 48, no. 12, 31 December 2007 (2007-12-31), pages 1296 - 1301 *
PEI, M. ET AL.: "Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs", OSTEOARTHRITIS AND CARTILAGE, vol. 17, 10 December 2008 (2008-12-10), pages 714 - 722 *

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Publication number Publication date
KR101649375B1 (ko) 2016-08-30
WO2011037416A9 (ko) 2011-09-01
KR20110032433A (ko) 2011-03-30
WO2011037416A2 (ko) 2011-03-31

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