JP2016529920A5 - - Google Patents
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- Publication number
- JP2016529920A5 JP2016529920A5 JP2016543968A JP2016543968A JP2016529920A5 JP 2016529920 A5 JP2016529920 A5 JP 2016529920A5 JP 2016543968 A JP2016543968 A JP 2016543968A JP 2016543968 A JP2016543968 A JP 2016543968A JP 2016529920 A5 JP2016529920 A5 JP 2016529920A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- ihep
- cell population
- asc
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims description 91
- 238000000034 method Methods 0.000 claims description 65
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 19
- 102000004140 Oncostatin M Human genes 0.000 claims description 10
- 108090000630 Oncostatin M Proteins 0.000 claims description 10
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 9
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 9
- 229940126864 fibroblast growth factor Drugs 0.000 claims description 9
- 210000003494 hepatocyte Anatomy 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000003550 marker Substances 0.000 claims description 9
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 claims description 6
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 claims description 6
- 102000013814 Wnt Human genes 0.000 claims description 6
- 108050003627 Wnt Proteins 0.000 claims description 6
- 239000000556 agonist Substances 0.000 claims description 6
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims description 6
- 239000002243 precursor Substances 0.000 claims description 6
- 230000011664 signaling Effects 0.000 claims description 6
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 claims description 5
- 229960003957 dexamethasone Drugs 0.000 claims description 5
- 241000124008 Mammalia Species 0.000 claims description 4
- 210000004185 liver Anatomy 0.000 claims description 4
- 230000003908 liver function Effects 0.000 claims description 4
- 102100028072 Fibroblast growth factor 4 Human genes 0.000 claims description 3
- 101001060274 Homo sapiens Fibroblast growth factor 4 Proteins 0.000 claims description 3
- 108010023082 activin A Proteins 0.000 claims description 3
- 239000011230 binding agent Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 230000009870 specific binding Effects 0.000 claims description 3
- 210000000130 stem cell Anatomy 0.000 claims description 3
- 238000004114 suspension culture Methods 0.000 claims description 3
- 210000001789 adipocyte Anatomy 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 7
- 230000006698 induction Effects 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 229960005489 paracetamol Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361880056P | 2013-09-19 | 2013-09-19 | |
| US61/880,056 | 2013-09-19 | ||
| PCT/US2014/056049 WO2015042125A2 (en) | 2013-09-19 | 2014-09-17 | Methods and compositions for producing hepatocyte-like cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020055548A Division JP7057797B2 (ja) | 2013-09-19 | 2020-03-26 | 肝細胞様細胞を産生するための方法および組成物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016529920A JP2016529920A (ja) | 2016-09-29 |
| JP2016529920A5 true JP2016529920A5 (enExample) | 2017-10-12 |
| JP6683615B2 JP6683615B2 (ja) | 2020-04-22 |
Family
ID=52689587
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016543968A Active JP6683615B2 (ja) | 2013-09-19 | 2014-09-17 | 肝細胞様細胞を産生するための方法および組成物 |
| JP2020055548A Active JP7057797B2 (ja) | 2013-09-19 | 2020-03-26 | 肝細胞様細胞を産生するための方法および組成物 |
| JP2022064376A Pending JP2022088668A (ja) | 2013-09-19 | 2022-04-08 | 肝細胞様細胞を産生するための方法および組成物 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020055548A Active JP7057797B2 (ja) | 2013-09-19 | 2020-03-26 | 肝細胞様細胞を産生するための方法および組成物 |
| JP2022064376A Pending JP2022088668A (ja) | 2013-09-19 | 2022-04-08 | 肝細胞様細胞を産生するための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10004767B2 (enExample) |
| EP (2) | EP3046628B1 (enExample) |
| JP (3) | JP6683615B2 (enExample) |
| CA (1) | CA2959117C (enExample) |
| DK (1) | DK3046628T3 (enExample) |
| ES (1) | ES2767062T3 (enExample) |
| SG (3) | SG11201707615YA (enExample) |
| WO (1) | WO2015042125A2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| WO2019071135A1 (en) * | 2017-10-05 | 2019-04-11 | The Johns Hopkins University | IMPLANTABLE BIOREACTOR AND METHODS OF MAKING AND USING |
| CN109749981B (zh) * | 2017-11-06 | 2023-10-10 | 博品(上海)生物医药科技有限公司 | 人源脂肪干细胞来源的肝细胞样细胞及其制备方法和应用 |
| SG11202101902QA (en) * | 2018-09-20 | 2021-04-29 | Novadip Biosciences | Biomaterial comprising adipose-derived stem cells and gelatin and method for producing the same |
| CA3175071A1 (en) | 2020-03-11 | 2021-09-16 | Bit Bio Limited | Method of generating hepatic cells |
| US20250102522A1 (en) | 2021-10-07 | 2025-03-27 | Hepatx Corporation | Methods of tracking donor cells in a recipient |
| US20230174949A1 (en) * | 2021-12-03 | 2023-06-08 | The Regents Of The University Of California | Engineered vascularized organoids |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4491632A (en) | 1979-10-22 | 1985-01-01 | The Massachusetts General Hospital | Process for producing antibodies to hepatitis virus and cell lines therefor |
| US4444887A (en) | 1979-12-10 | 1984-04-24 | Sloan-Kettering Institute | Process for making human antibody producing B-lymphocytes |
| EP0043718B1 (en) | 1980-07-07 | 1984-11-28 | National Research Development Corporation | Improvements in or relating to cell lines |
| DE3402927A1 (de) | 1984-01-28 | 1985-08-08 | Pfeifer & Langen, 5000 Köln | Zellkulturmikrotraeger, verfahren zu seiner herstellung und seine verwendung zum zuechten von verankerungsabhaengigen zellen |
| US5026650A (en) | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5631734A (en) | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US6214618B1 (en) | 1998-04-07 | 2001-04-10 | Solohill Engineering, Inc. | Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior |
| US6712610B2 (en) | 1999-04-02 | 2004-03-30 | Forsyth Dental Infirmary For Children | Characterization of an antibiotic impregnated delivery system as an intracanal medicament in endodontic therapy and method |
| EP1434857B1 (en) | 2001-10-02 | 2007-08-01 | Novo Nordisk Health Care AG | Method for production of recombinant proteins in eukaryote cells |
| HUE032238T2 (en) * | 2002-03-15 | 2017-09-28 | Univ North Carolina Chapel Hill | Primitive and proximal liver stem cells |
| JP4907843B2 (ja) | 2002-03-25 | 2012-04-04 | 公益財団法人ひろしま産業振興機構 | ヒト肝細胞増殖方法とヒト肝細胞の取得方法 |
| US7670839B2 (en) | 2002-12-23 | 2010-03-02 | Fuji Film Manufacturing Europe B.V. | Process for coating cell-culture support |
| US7813977B2 (en) | 2003-09-11 | 2010-10-12 | Bgc Partners, Inc. | Graphical user interface related to non-standard trading of financial instruments |
| US7361493B1 (en) | 2004-05-26 | 2008-04-22 | The United States Of America As Represented By The Secretary Of The Department Of Veterans Affairs | Production of urokinase in a three-dimensional cell culture |
| US20070298016A1 (en) * | 2004-09-29 | 2007-12-27 | Nico Heins | Methods For The Generation Of Hepatocyte-Like Cells From Human Blastocyst-Derived Stem (Hbs) |
| US8592209B2 (en) | 2006-01-30 | 2013-11-26 | University Of Virginia Patent Foundation | Methods of preparing and characterizing mesenchymal stem cell aggregates and uses thereof |
| DK2019683T4 (da) | 2006-04-25 | 2022-08-29 | Univ California | Indgivelse af vækstfaktorer til behandling af CNS-lidelser |
| EP2019859A4 (en) * | 2006-04-25 | 2009-11-25 | Univ Louisiana State | ADULT FAMILY CELLS FROM FAT TISSUE FOR LIVER REGENERATION |
| US7989204B2 (en) * | 2006-04-28 | 2011-08-02 | Viacyte, Inc. | Hepatocyte lineage cells |
| CN101448527B (zh) * | 2006-05-19 | 2013-12-18 | 香港大学 | 细胞-基质微球，制备方法和应用 |
| US8158108B2 (en) | 2006-06-28 | 2012-04-17 | S.C. Johnson & Son, Inc. | VOC-free compressed gas aerosol compositions |
| WO2008060360A2 (en) | 2006-09-28 | 2008-05-22 | Surmodics, Inc. | Implantable medical device with apertures for delivery of bioactive agents |
| EP2155065A4 (en) * | 2007-05-14 | 2014-09-10 | Univ Johns Hopkins | PROCESS FOR THE IMAGE DISPLAY OF CELLS IN VIVO |
| JP2010534065A (ja) * | 2007-07-20 | 2010-11-04 | セルアーティス アーベー | ヒト胚盤胞幹細胞に、胚体内胚葉（ＤＥ‐ｈｅｐ）を介して由来する新規な肝細胞の集団 |
| AU2009205886B2 (en) | 2008-01-18 | 2015-08-27 | Katholieke Universiteit Leuven | Stem cell aggregates and methods for making and using |
| WO2009097468A2 (en) | 2008-01-29 | 2009-08-06 | Kliman Gilbert H | Drug delivery devices, kits and methods therefor |
| US20100112031A1 (en) | 2008-10-17 | 2010-05-06 | University Of Virginia Patent Foundation | Compositions And Methods For Regulating Extracellular Matrix Production In Adipose Derived Cells |
| US20100098739A1 (en) | 2008-10-20 | 2010-04-22 | University Of Virginia Patent Foundation | Compositions and methods for modular soft tissue repair |
| WO2010049752A1 (en) * | 2008-10-31 | 2010-05-06 | Katholieke Universiteit Leuven | Optimized methods for differentiation of cells into cells with hepatocyte and hepatocyte progenitor phenotypes, cells produced by the methods, and methods for using the cells |
| US8895300B2 (en) * | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
| KR101774546B1 (ko) | 2008-11-20 | 2017-09-04 | 얀센 바이오테크 인코포레이티드 | 마이크로-캐리어 상의 만능 줄기 세포 배양 |
| KR101017710B1 (ko) * | 2009-02-05 | 2011-02-25 | 한양대학교 산학협력단 | 세포 스페로이드 형태의 이식용 성체 줄기세포 집합체 및 그 제조방법 |
| WO2010108126A2 (en) * | 2009-03-19 | 2010-09-23 | Fate Therapeutics, Inc. | Reprogramming compositions and methods of using the same |
| TW201042038A (en) | 2009-05-28 | 2010-12-01 | Corning Inc | Synthetic microcarriers for culturing cells |
| JP2011013535A (ja) | 2009-07-03 | 2011-01-20 | Olympus Corp | 光調節装置及び光学系 |
| WO2011006205A1 (en) * | 2009-07-15 | 2011-01-20 | Regenertech Pty Limited | Method of producing progenitor cells from differentiated cells |
| CN101914495A (zh) | 2010-07-22 | 2010-12-15 | 吉林大学 | 用于体外大量扩增毛囊干细胞的培养方法 |
| ITMI20111096A1 (it) * | 2011-06-17 | 2012-12-18 | Filosa Stefania | Cellule dell'endoderma da cellule pluripotenti |
| US9215088B2 (en) | 2011-06-29 | 2015-12-15 | Broadcom Corporation | Identification of application sessions |
| WO2013018851A1 (ja) * | 2011-08-02 | 2013-02-07 | 独立行政法人国立がん研究センター | 誘導肝幹細胞から肝分化誘導する方法及び誘導肝前駆細胞 |
| EP2554176B1 (en) * | 2011-08-04 | 2016-07-27 | ETHIANUM Betriebsgesellschaft mbH & Co. KG | Means for liver regeneration |
-
2014
- 2014-09-17 SG SG11201707615YA patent/SG11201707615YA/en unknown
- 2014-09-17 JP JP2016543968A patent/JP6683615B2/ja active Active
- 2014-09-17 EP EP14846200.5A patent/EP3046628B1/en active Active
- 2014-09-17 SG SG10201914005TA patent/SG10201914005TA/en unknown
- 2014-09-17 WO PCT/US2014/056049 patent/WO2015042125A2/en not_active Ceased
- 2014-09-17 US US14/917,558 patent/US10004767B2/en active Active
- 2014-09-17 ES ES14846200T patent/ES2767062T3/es active Active
- 2014-09-17 DK DK14846200.5T patent/DK3046628T3/da active
- 2014-09-17 EP EP19191062.9A patent/EP3685884A1/en not_active Withdrawn
- 2014-09-17 SG SG10201802231RA patent/SG10201802231RA/en unknown
- 2014-09-17 CA CA2959117A patent/CA2959117C/en active Active
-
2018
- 2018-05-08 US US15/974,377 patent/US11291691B2/en active Active
-
2020
- 2020-03-26 JP JP2020055548A patent/JP7057797B2/ja active Active
-
2022
- 2022-02-15 US US17/672,528 patent/US20220168359A1/en not_active Abandoned
- 2022-04-08 JP JP2022064376A patent/JP2022088668A/ja active Pending
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