JP2011503208A5 - - Google Patents
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- JP2011503208A5 JP2011503208A5 JP2010534253A JP2010534253A JP2011503208A5 JP 2011503208 A5 JP2011503208 A5 JP 2011503208A5 JP 2010534253 A JP2010534253 A JP 2010534253A JP 2010534253 A JP2010534253 A JP 2010534253A JP 2011503208 A5 JP2011503208 A5 JP 2011503208A5
- Authority
- JP
- Japan
- Prior art keywords
- hpv
- brij
- protein
- polyoxyethylene
- och
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims description 125
- 239000003153 chemical reaction reagent Substances 0.000 claims description 78
- 241000701806 Human papillomavirus Species 0.000 claims description 73
- 102000004169 proteins and genes Human genes 0.000 claims description 73
- 108090000623 proteins and genes Proteins 0.000 claims description 73
- -1 polyoxyethylene Polymers 0.000 claims description 60
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 52
- 238000000605 extraction Methods 0.000 claims description 42
- 230000003472 neutralizing effect Effects 0.000 claims description 31
- 239000004094 surface-active agent Substances 0.000 claims description 30
- 239000000284 extract Substances 0.000 claims description 29
- 239000000203 mixture Substances 0.000 claims description 28
- 238000003556 assay Methods 0.000 claims description 21
- 150000005215 alkyl ethers Chemical class 0.000 claims description 19
- 108010067390 Viral Proteins Proteins 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- HBXWUCXDUUJDRB-UHFFFAOYSA-N 1-octadecoxyoctadecane Chemical compound CCCCCCCCCCCCCCCCCCOCCCCCCCCCCCCCCCCCC HBXWUCXDUUJDRB-UHFFFAOYSA-N 0.000 claims description 15
- FFJCNSLCJOQHKM-CLFAGFIQSA-N (z)-1-[(z)-octadec-9-enoxy]octadec-9-ene Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCCCCCCC\C=C/CCCCCCCC FFJCNSLCJOQHKM-CLFAGFIQSA-N 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 12
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
- FDCJDKXCCYFOCV-UHFFFAOYSA-N 1-hexadecoxyhexadecane Chemical compound CCCCCCCCCCCCCCCCOCCCCCCCCCCCCCCCC FDCJDKXCCYFOCV-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- DTPCFIHYWYONMD-UHFFFAOYSA-N decaethylene glycol Polymers OCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO DTPCFIHYWYONMD-UHFFFAOYSA-N 0.000 claims description 9
- 102000000470 PDZ domains Human genes 0.000 claims description 8
- 108050008994 PDZ domains Proteins 0.000 claims description 8
- 241000700605 Viruses Species 0.000 claims description 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- CMCBDXRRFKYBDG-UHFFFAOYSA-N 1-dodecoxydodecane Chemical compound CCCCCCCCCCCCOCCCCCCCCCCCC CMCBDXRRFKYBDG-UHFFFAOYSA-N 0.000 claims description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 238000002965 ELISA Methods 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 239000002033 PVDF binder Substances 0.000 claims description 6
- 239000004793 Polystyrene Substances 0.000 claims description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 6
- 239000011324 bead Substances 0.000 claims description 6
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 6
- 229910052737 gold Inorganic materials 0.000 claims description 6
- 239000010931 gold Substances 0.000 claims description 6
- 229920002223 polystyrene Polymers 0.000 claims description 6
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 6
- 239000000975 dye Substances 0.000 claims description 5
- 229920001184 polypeptide Polymers 0.000 claims description 5
- 229920000136 polysorbate Polymers 0.000 claims description 5
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 5
- 239000000377 silicon dioxide Substances 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical group CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 241000725643 Respiratory syncytial virus Species 0.000 claims description 4
- 241000700584 Simplexvirus Species 0.000 claims description 4
- 150000001299 aldehydes Chemical class 0.000 claims description 4
- 239000000834 fixative Substances 0.000 claims description 4
- 150000002576 ketones Chemical class 0.000 claims description 4
- 238000006386 neutralization reaction Methods 0.000 claims description 4
- 230000001717 pathogenic effect Effects 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- WYZGMTDJCGEDAQ-UHFFFAOYSA-N 2-ethylbut-3-enoic acid Chemical compound CCC(C=C)C(O)=O WYZGMTDJCGEDAQ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000020 Nitrocellulose Substances 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 3
- 229920002845 Poly(methacrylic acid) Polymers 0.000 claims description 3
- 239000004952 Polyamide Substances 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 238000001069 Raman spectroscopy Methods 0.000 claims description 3
- 229920000297 Rayon Polymers 0.000 claims description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 claims description 3
- OMUOMODZGKSORV-UVTDQMKNSA-N aurone Chemical compound O1C2=CC=CC=C2C(=O)\C1=C\C1=CC=CC=C1 OMUOMODZGKSORV-UVTDQMKNSA-N 0.000 claims description 3
- 229930015036 aurone Natural products 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 229920002301 cellulose acetate Polymers 0.000 claims description 3
- 229920001577 copolymer Polymers 0.000 claims description 3
- 238000003018 immunoassay Methods 0.000 claims description 3
- 239000004816 latex Substances 0.000 claims description 3
- 229920000126 latex Polymers 0.000 claims description 3
- 229920001220 nitrocellulos Polymers 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- YWAKXRMUMFPDSH-UHFFFAOYSA-N pentene Chemical compound CCCC=C YWAKXRMUMFPDSH-UHFFFAOYSA-N 0.000 claims description 3
- 229920002647 polyamide Polymers 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 229920000728 polyester Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 3
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 3
- 229920006324 polyoxymethylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 229920002689 polyvinyl acetate Polymers 0.000 claims description 3
- 239000011118 polyvinyl acetate Substances 0.000 claims description 3
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 3
- 239000004800 polyvinyl chloride Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000002964 rayon Substances 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 229910052709 silver Inorganic materials 0.000 claims description 3
- 239000004332 silver Substances 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical group OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 3
- 229940038773 trisodium citrate Drugs 0.000 claims description 3
- FBKFDSUZBVDZIX-FFGDOFBPSA-N (4s)-4-[[(2s)-2-[[(2s)-2-[[2-[[(2s)-2-[[(2s)-2-acetamido-3-phenylpropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-2-methylpropanoyl]amino]-3-[4-(phosphonomethyl)phenyl]propanoyl]amino]-3-(6-chloro-1h-indol-3-yl)propanoyl]amino]-5-[[1-[[(2s)-1-amino-4-meth Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NC(C)(C)C(=O)N[C@@H](CC=1C=CC(CP(O)(O)=O)=CC=1)C(=O)N[C@@H](CC=1C2=CC=C(Cl)C=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)NC1(CC1)C(=O)N[C@@H](CC(C)C)C(N)=O)NC(C)=O)C1=CC=CC=C1 FBKFDSUZBVDZIX-FFGDOFBPSA-N 0.000 claims description 2
- 101100489349 Arabidopsis thaliana PAT24 gene Proteins 0.000 claims description 2
- 241001115402 Ebolavirus Species 0.000 claims description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 2
- 101100315589 Homo sapiens TAX1BP3 gene Proteins 0.000 claims description 2
- 241001115401 Marburgvirus Species 0.000 claims description 2
- 102100027240 Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 Human genes 0.000 claims description 2
- 101710137278 Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 Proteins 0.000 claims description 2
- 101100313925 Oryza sativa subsp. japonica TIP1-2 gene Proteins 0.000 claims description 2
- 101100313932 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TIP20 gene Proteins 0.000 claims description 2
- 241000580858 Simian-Human immunodeficiency virus Species 0.000 claims description 2
- 102100036221 Tax1-binding protein 3 Human genes 0.000 claims description 2
- 101100313921 Zea mays TIP1-1 gene Proteins 0.000 claims description 2
- 150000001298 alcohols Chemical class 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000003365 glass fiber Substances 0.000 claims description 2
- 229910001385 heavy metal Inorganic materials 0.000 claims description 2
- 208000006454 hepatitis Diseases 0.000 claims description 2
- 231100000283 hepatitis Toxicity 0.000 claims description 2
- 238000007689 inspection Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000012285 osmium tetroxide Substances 0.000 claims description 2
- 229910000489 osmium tetroxide Inorganic materials 0.000 claims description 2
- 239000000123 paper Substances 0.000 claims description 2
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims description 2
- 101150023068 tip1 gene Proteins 0.000 claims description 2
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 claims 1
- 230000000711 cancerogenic effect Effects 0.000 claims 1
- 231100000315 carcinogenic Toxicity 0.000 claims 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 229940016590 sarkosyl Drugs 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/985,547 | 2007-11-14 | ||
| US11/985,547 US9207240B2 (en) | 2007-11-14 | 2007-11-14 | Method of efficient extraction of protein from cells |
| PCT/US2008/083707 WO2009065091A1 (en) | 2007-11-14 | 2008-11-14 | Method of efficient extraction of protein from cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011503208A JP2011503208A (ja) | 2011-01-27 |
| JP2011503208A5 true JP2011503208A5 (https=) | 2012-01-05 |
| JP5787519B2 JP5787519B2 (ja) | 2015-09-30 |
Family
ID=40624059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010534253A Expired - Fee Related JP5787519B2 (ja) | 2007-11-14 | 2008-11-14 | 細胞からの効率的なタンパク質抽出方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US9207240B2 (https=) |
| EP (1) | EP2222838B1 (https=) |
| JP (1) | JP5787519B2 (https=) |
| CN (2) | CN107629109A (https=) |
| AU (1) | AU2008322473B2 (https=) |
| BR (1) | BRPI0820177B8 (https=) |
| CA (1) | CA2705765C (https=) |
| ES (1) | ES2456341T3 (https=) |
| WO (1) | WO2009065091A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009536958A (ja) | 2006-05-11 | 2009-10-22 | ベクトン・ディキンソン・アンド・カンパニー | 細胞からのタンパク質抽出方法 |
| US8278056B2 (en) * | 2008-06-13 | 2012-10-02 | Oncohealth Corp. | Detection of early stages and late stages HPV infection |
| US9207240B2 (en) | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
| EP2473596B1 (en) * | 2009-09-03 | 2017-12-13 | Becton, Dickinson and Company | Methods and compositions for direct chemical lysis |
| US8658417B2 (en) * | 2009-09-15 | 2014-02-25 | Qiagen Gaithersburg, Inc. | Multiple-input analytical system |
| US8994937B2 (en) | 2012-04-18 | 2015-03-31 | Hewlett-Packard Development Company, L.P. | Surface enhanced Raman spectroscopy calibration curve generating systems |
| US9680916B2 (en) | 2013-08-01 | 2017-06-13 | Flowtraq, Inc. | Methods and systems for distribution and retrieval of network traffic records |
| JP6397224B2 (ja) * | 2014-06-04 | 2018-09-26 | 田中貴金属工業株式会社 | 免疫学的測定試薬におけるプロゾーン現象の解消法 |
| CN104237198A (zh) * | 2014-07-31 | 2014-12-24 | 中国人民解放军第二军医大学 | 一种判定中药材或中药饮片是否染色的方法 |
| JP2019523863A (ja) * | 2016-05-31 | 2019-08-29 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ウイルス抗原の血清学的検出方法 |
| CN107621462B (zh) * | 2016-07-13 | 2020-03-31 | 王志伟 | 一种组织透明化液sut及其制备和应用 |
| CN108300705B (zh) * | 2017-01-12 | 2021-10-22 | 厦门大学 | 稳定呼吸道合胞病毒融合蛋白的方法 |
| WO2019121235A1 (en) * | 2017-12-22 | 2019-06-27 | Basf Plant Science Company Gmbh | Method and means for an isolation of membrane-bound proteins from a biological sample, preferably processed plant seed meal |
| JP7267548B2 (ja) * | 2019-04-24 | 2023-05-02 | 株式会社ニップン | トマト黄化葉巻ウイルス（ｔｙｌｃｖ）の免疫学的診断法 |
| CN112213164A (zh) * | 2019-07-12 | 2021-01-12 | 温州医科大学附属第一医院 | 一种总蛋白提取试剂盒及提取方法 |
| CN116223793A (zh) * | 2023-02-14 | 2023-06-06 | 上海乐备实生物技术有限公司 | 一种用于外泌体中补体检测的裂解液及其应用 |
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| US9207240B2 (en) | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
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2007
- 2007-11-14 US US11/985,547 patent/US9207240B2/en active Active
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2008
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- 2008-11-14 JP JP2010534253A patent/JP5787519B2/ja not_active Expired - Fee Related
- 2008-11-14 WO PCT/US2008/083707 patent/WO2009065091A1/en not_active Ceased
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2015
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