CN107629109A - 从细胞中有效提取蛋白质的方法 - Google Patents
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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Landscapes
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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| US8962262B2 (en) * | 2006-05-11 | 2015-02-24 | Arbor Vita Corporation | Method of protein extraction from cells |
| US20100003704A1 (en) * | 2008-06-13 | 2010-01-07 | Shuling Cheng | IN SITU detection of early stages and late stages HPV infection |
| US9207240B2 (en) | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
| BR112012004699B1 (pt) | 2009-09-03 | 2019-10-15 | Becton, Dickinson And Company | Métodos, composições e kits para lise química direta |
| US8282895B2 (en) * | 2009-09-15 | 2012-10-09 | Qiagen Gaithersburg, Inc. | Reagent cabinet system |
| US8994937B2 (en) | 2012-04-18 | 2015-03-31 | Hewlett-Packard Development Company, L.P. | Surface enhanced Raman spectroscopy calibration curve generating systems |
| US9680916B2 (en) | 2013-08-01 | 2017-06-13 | Flowtraq, Inc. | Methods and systems for distribution and retrieval of network traffic records |
| JP6397224B2 (ja) * | 2014-06-04 | 2018-09-26 | 田中貴金属工業株式会社 | 免疫学的測定試薬におけるプロゾーン現象の解消法 |
| CN104237198A (zh) * | 2014-07-31 | 2014-12-24 | 中国人民解放军第二军医大学 | 一种判定中药材或中药饮片是否染色的方法 |
| EP3465217B1 (en) * | 2016-05-31 | 2025-09-03 | Roche Diagnostics GmbH | Method for serological detection of viral antigens |
| CN107621462B (zh) * | 2016-07-13 | 2020-03-31 | 王志伟 | 一种组织透明化液sut及其制备和应用 |
| WO2018130072A1 (zh) * | 2017-01-12 | 2018-07-19 | 厦门大学 | 稳定呼吸道合胞病毒融合蛋白的方法 |
| BR112020012490A2 (pt) * | 2017-12-22 | 2020-11-24 | Basf Plant Science Company Gmbh | método para a extração de proteínas ligadas à membrana e composição |
| JP7267548B2 (ja) * | 2019-04-24 | 2023-05-02 | 株式会社ニップン | トマト黄化葉巻ウイルス(tylcv)の免疫学的診断法 |
| CN112213164A (zh) * | 2019-07-12 | 2021-01-12 | 温州医科大学附属第一医院 | 一种总蛋白提取试剂盒及提取方法 |
| CN116223793A (zh) * | 2023-02-14 | 2023-06-06 | 上海乐备实生物技术有限公司 | 一种用于外泌体中补体检测的裂解液及其应用 |
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| CN1413195A (zh) * | 1999-12-08 | 2003-04-23 | 辛根塔参与股份公司 | 用于新烟碱基杀虫剂的免疫测定法 |
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| CN1984989A (zh) * | 2004-06-09 | 2007-06-20 | 病原体切除与诊断科技公司 | 用于从样品中除去靶分析物的包埋在多孔底物中的颗粒 |
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2007
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Patent Citations (4)
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| EP0363110A2 (en) * | 1988-10-07 | 1990-04-11 | Johnson & Johnson Clinical Diagnostics, Inc. | High pH extraction composition and its use to determine a chlamydial, gonococcal or herpes antigen |
| CN1413195A (zh) * | 1999-12-08 | 2003-04-23 | 辛根塔参与股份公司 | 用于新烟碱基杀虫剂的免疫测定法 |
| US20040101947A1 (en) * | 2002-11-01 | 2004-05-27 | Promega Corporation | Cell lysis composition, methods of use, apparatus and kit |
| CN1984989A (zh) * | 2004-06-09 | 2007-06-20 | 病原体切除与诊断科技公司 | 用于从样品中除去靶分析物的包埋在多孔底物中的颗粒 |
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| ES2456341T3 (es) | 2014-04-22 |
| US9207240B2 (en) | 2015-12-08 |
| BRPI0820177A2 (pt) | 2014-11-04 |
| US9995745B2 (en) | 2018-06-12 |
| US20160216258A1 (en) | 2016-07-28 |
| JP5787519B2 (ja) | 2015-09-30 |
| JP2011503208A (ja) | 2011-01-27 |
| CN101910400B (zh) | 2017-06-27 |
| CA2705765A1 (en) | 2009-05-22 |
| AU2008322473A1 (en) | 2009-05-22 |
| BRPI0820177B8 (pt) | 2021-07-27 |
| US20090123910A1 (en) | 2009-05-14 |
| AU2008322473B2 (en) | 2014-07-03 |
| WO2009065091A1 (en) | 2009-05-22 |
| EP2222838A1 (en) | 2010-09-01 |
| CA2705765C (en) | 2018-12-11 |
| EP2222838B1 (en) | 2014-01-22 |
| BRPI0820177B1 (pt) | 2019-07-02 |
| CN101910400A (zh) | 2010-12-08 |
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