CN107629109A - 从细胞中有效提取蛋白质的方法 - Google Patents

从细胞中有效提取蛋白质的方法 Download PDF

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Publication number
CN107629109A
CN107629109A CN201710593190.6A CN201710593190A CN107629109A CN 107629109 A CN107629109 A CN 107629109A CN 201710593190 A CN201710593190 A CN 201710593190A CN 107629109 A CN107629109 A CN 107629109A
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CN
China
Prior art keywords
cell
protein
reagent
hpv
brij
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Pending
Application number
CN201710593190.6A
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English (en)
Chinese (zh)
Inventor
阿德里安·P·马利克
弗吉尼亚·M·克鲁斯
朱莉·L·罗萨莱斯
卡里·S·弗格森
杰弗里·H·布鲁顿
罗伯特·J·比登考普夫
约翰·曼特罗
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Becton Dickinson and Co
Arbor Vita Corp
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Becton Dickinson and Co
Arbor Vita Corp
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Publication of CN107629109A publication Critical patent/CN107629109A/zh
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4044Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/20011Papillomaviridae
    • C12N2710/20022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01DNA viruses
    • G01N2333/025Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16HIV-1, HIV-2
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00Immunoassays for the detection of microorganisms
    • G01N2469/10Detection of antigens from microorganism in sample from host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
CN201710593190.6A 2007-11-14 2008-11-14 从细胞中有效提取蛋白质的方法 Pending CN107629109A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/985,547 2007-11-14
US11/985,547 US9207240B2 (en) 2007-11-14 2007-11-14 Method of efficient extraction of protein from cells
CN200880124680.0A CN101910400B (zh) 2007-11-14 2008-11-14 从细胞中有效提取蛋白质的方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN200880124680.0A Division CN101910400B (zh) 2007-11-14 2008-11-14 从细胞中有效提取蛋白质的方法

Publications (1)

Publication Number Publication Date
CN107629109A true CN107629109A (zh) 2018-01-26

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CN201710593190.6A Pending CN107629109A (zh) 2007-11-14 2008-11-14 从细胞中有效提取蛋白质的方法
CN200880124680.0A Expired - Fee Related CN101910400B (zh) 2007-11-14 2008-11-14 从细胞中有效提取蛋白质的方法

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Country Status (9)

Country Link
US (2) US9207240B2 (https=)
EP (1) EP2222838B1 (https=)
JP (1) JP5787519B2 (https=)
CN (2) CN107629109A (https=)
AU (1) AU2008322473B2 (https=)
BR (1) BRPI0820177B8 (https=)
CA (1) CA2705765C (https=)
ES (1) ES2456341T3 (https=)
WO (1) WO2009065091A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8962262B2 (en) * 2006-05-11 2015-02-24 Arbor Vita Corporation Method of protein extraction from cells
US20100003704A1 (en) * 2008-06-13 2010-01-07 Shuling Cheng IN SITU detection of early stages and late stages HPV infection
US9207240B2 (en) 2007-11-14 2015-12-08 Arbor Vita Corporation Method of efficient extraction of protein from cells
BR112012004699B1 (pt) 2009-09-03 2019-10-15 Becton, Dickinson And Company Métodos, composições e kits para lise química direta
US8282895B2 (en) * 2009-09-15 2012-10-09 Qiagen Gaithersburg, Inc. Reagent cabinet system
US8994937B2 (en) 2012-04-18 2015-03-31 Hewlett-Packard Development Company, L.P. Surface enhanced Raman spectroscopy calibration curve generating systems
US9680916B2 (en) 2013-08-01 2017-06-13 Flowtraq, Inc. Methods and systems for distribution and retrieval of network traffic records
JP6397224B2 (ja) * 2014-06-04 2018-09-26 田中貴金属工業株式会社 免疫学的測定試薬におけるプロゾーン現象の解消法
CN104237198A (zh) * 2014-07-31 2014-12-24 中国人民解放军第二军医大学 一种判定中药材或中药饮片是否染色的方法
EP3465217B1 (en) * 2016-05-31 2025-09-03 Roche Diagnostics GmbH Method for serological detection of viral antigens
CN107621462B (zh) * 2016-07-13 2020-03-31 王志伟 一种组织透明化液sut及其制备和应用
WO2018130072A1 (zh) * 2017-01-12 2018-07-19 厦门大学 稳定呼吸道合胞病毒融合蛋白的方法
BR112020012490A2 (pt) * 2017-12-22 2020-11-24 Basf Plant Science Company Gmbh método para a extração de proteínas ligadas à membrana e composição
JP7267548B2 (ja) * 2019-04-24 2023-05-02 株式会社ニップン トマト黄化葉巻ウイルス(tylcv)の免疫学的診断法
CN112213164A (zh) * 2019-07-12 2021-01-12 温州医科大学附属第一医院 一种总蛋白提取试剂盒及提取方法
CN116223793A (zh) * 2023-02-14 2023-06-06 上海乐备实生物技术有限公司 一种用于外泌体中补体检测的裂解液及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363110A2 (en) * 1988-10-07 1990-04-11 Johnson & Johnson Clinical Diagnostics, Inc. High pH extraction composition and its use to determine a chlamydial, gonococcal or herpes antigen
CN1413195A (zh) * 1999-12-08 2003-04-23 辛根塔参与股份公司 用于新烟碱基杀虫剂的免疫测定法
US20040101947A1 (en) * 2002-11-01 2004-05-27 Promega Corporation Cell lysis composition, methods of use, apparatus and kit
CN1984989A (zh) * 2004-06-09 2007-06-20 病原体切除与诊断科技公司 用于从样品中除去靶分析物的包埋在多孔底物中的颗粒

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3546334A (en) * 1965-05-21 1970-12-08 Lerner Lab Inc Composition for fixing and protecting a smear of body cells and method of applying same
US3303038A (en) * 1965-07-29 1967-02-07 Ethicon Inc Process of forming collagen articles and dispersions
JPS5535088B2 (https=) * 1971-09-10 1980-09-11
US4578282A (en) * 1982-02-04 1986-03-25 Harrison James S Composition for diagnostic reagents
US4766065A (en) 1984-08-23 1988-08-23 Becton Dickinson And Company Detection of cell membrane protein
US4649192A (en) * 1985-05-30 1987-03-10 Smith Kline-Rit Method for the isolation and purification of hepatitis B surface antigen using polysorbate
US4857300A (en) * 1987-07-27 1989-08-15 Cytocorp, Inc. Cytological and histological fixative formulation and methods for using same
US5047325A (en) 1988-10-07 1991-09-10 Eastman Kodak Company Wash solution containing a cationic surfactant and its use in chlamydial and gonococcal determinations
US5081010A (en) 1989-02-09 1992-01-14 Eastman Kodak Company Extraction composition, test kit and their use to extract or determine herpes simplex viral antigen
US5104640A (en) * 1989-03-17 1992-04-14 Wescor, Inc. Fixative composition for fixing blood smears to slides
US5256571A (en) * 1991-05-01 1993-10-26 Cytyc Corporation Cell preservative solution
US5196182A (en) * 1991-05-08 1993-03-23 Streck Laboratories, Inc. Tissue fixative
US5773277A (en) * 1992-06-26 1998-06-30 Seikagaku Kogyo Kabushiki Kaisha Crystalline chondroitinase isolated from Proteus vulgaris ATCC 6896
US5432056A (en) * 1993-11-15 1995-07-11 Ventana Medical Systems, Inc. Bisulfite-based tissue fixative
US5658749A (en) * 1994-04-05 1997-08-19 Corning Clinical Laboratories, Inc. Method for processing mycobacteria
US5620869A (en) * 1995-09-28 1997-04-15 Becton, Dickinson And Company Methods for reducing inhibition of nucleic acid amplification reactions
US7306926B2 (en) * 1998-07-01 2007-12-11 Mtm Laboratories Ag Method for detecting carcinomas in a solubilized cervical body sample
US6245568B1 (en) 1999-03-26 2001-06-12 Merck & Co., Inc. Human papilloma virus vaccine with disassembled and reassembled virus-like particles
US20050019841A1 (en) * 1999-05-14 2005-01-27 Arbor Vita Corporation Modulation of signaling pathways
US20060148711A1 (en) * 1999-05-14 2006-07-06 Arbor Vita Corporation Molecular interactions in neurons
US20050037969A1 (en) * 1999-05-14 2005-02-17 Arbor Vita Corporation Molecular interactions in hematopoietic cells
US6488671B1 (en) * 1999-10-22 2002-12-03 Corazon Technologies, Inc. Methods for enhancing fluid flow through an obstructed vascular site, and systems and kits for use in practicing the same
US6337189B1 (en) * 2000-02-08 2002-01-08 Streck Laboratories, Inc. Fixative system, method and composition for biological testing
US6436662B1 (en) * 2000-04-04 2002-08-20 Digene Corporation Device and method for cytology slide preparation
WO2002007751A1 (en) * 2000-07-25 2002-01-31 Axcell Biosciences Corporation Identification and isolation of novel polypeptides having pdz domains and methods of using same
US20030175852A1 (en) * 2000-09-15 2003-09-18 Kalra Krishan L Ehancement of in situ hybridization
US7312041B2 (en) * 2001-02-16 2007-12-25 Arbor Vita Corporation Methods of diagnosing cervical cancer
DK1402015T3 (da) 2001-06-22 2011-12-05 Hoffmann La Roche Opløseligt kompleks omfattende et retroviralt overfladeglycoprotein og FkpA eller SlyD
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
BR0314155A (pt) 2002-09-09 2005-07-05 Arbor Vita Corp Métodos para diagnosticar câncer cervical
DE60300339T2 (de) 2003-08-25 2006-04-13 Mtm Laboratories Ag Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben
DE602004026912D1 (de) * 2003-12-16 2010-06-10 Qiagen North American Holdings Formulierungen und verfahren zum denaturieren von proteinen
JP5030594B2 (ja) * 2003-12-23 2012-09-19 アルボー ビータ コーポレーション Hpvの発癌性株に対する抗体およびそれらの使用方法
US7723044B2 (en) * 2004-04-15 2010-05-25 Allied Biotech, Inc. Methods and apparatus for detection of viral infection
US8962262B2 (en) * 2006-05-11 2015-02-24 Arbor Vita Corporation Method of protein extraction from cells
US9207240B2 (en) 2007-11-14 2015-12-08 Arbor Vita Corporation Method of efficient extraction of protein from cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363110A2 (en) * 1988-10-07 1990-04-11 Johnson & Johnson Clinical Diagnostics, Inc. High pH extraction composition and its use to determine a chlamydial, gonococcal or herpes antigen
CN1413195A (zh) * 1999-12-08 2003-04-23 辛根塔参与股份公司 用于新烟碱基杀虫剂的免疫测定法
US20040101947A1 (en) * 2002-11-01 2004-05-27 Promega Corporation Cell lysis composition, methods of use, apparatus and kit
CN1984989A (zh) * 2004-06-09 2007-06-20 病原体切除与诊断科技公司 用于从样品中除去靶分析物的包埋在多孔底物中的颗粒

Also Published As

Publication number Publication date
EP2222838A4 (en) 2011-01-05
ES2456341T3 (es) 2014-04-22
US9207240B2 (en) 2015-12-08
BRPI0820177A2 (pt) 2014-11-04
US9995745B2 (en) 2018-06-12
US20160216258A1 (en) 2016-07-28
JP5787519B2 (ja) 2015-09-30
JP2011503208A (ja) 2011-01-27
CN101910400B (zh) 2017-06-27
CA2705765A1 (en) 2009-05-22
AU2008322473A1 (en) 2009-05-22
BRPI0820177B8 (pt) 2021-07-27
US20090123910A1 (en) 2009-05-14
AU2008322473B2 (en) 2014-07-03
WO2009065091A1 (en) 2009-05-22
EP2222838A1 (en) 2010-09-01
CA2705765C (en) 2018-12-11
EP2222838B1 (en) 2014-01-22
BRPI0820177B1 (pt) 2019-07-02
CN101910400A (zh) 2010-12-08

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