CA2705765C - Method of efficient extraction of protein from cells - Google Patents
Method of efficient extraction of protein from cells Download PDFInfo
- Publication number
- CA2705765C CA2705765C CA2705765A CA2705765A CA2705765C CA 2705765 C CA2705765 C CA 2705765C CA 2705765 A CA2705765 A CA 2705765A CA 2705765 A CA2705765 A CA 2705765A CA 2705765 C CA2705765 C CA 2705765C
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- protein
- hpv
- cells
- brij
- polyoxyethylene
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
- G01N2333/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
- G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
- G01N2333/16—HIV-1, HIV-2
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/10—Detection of antigens from microorganism in sample from host
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/985,547 | 2007-11-14 | ||
| US11/985,547 US9207240B2 (en) | 2007-11-14 | 2007-11-14 | Method of efficient extraction of protein from cells |
| PCT/US2008/083707 WO2009065091A1 (en) | 2007-11-14 | 2008-11-14 | Method of efficient extraction of protein from cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2705765A1 CA2705765A1 (en) | 2009-05-22 |
| CA2705765C true CA2705765C (en) | 2018-12-11 |
Family
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|---|---|---|---|
| CA2705765A Expired - Fee Related CA2705765C (en) | 2007-11-14 | 2008-11-14 | Method of efficient extraction of protein from cells |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US9207240B2 (https=) |
| EP (1) | EP2222838B1 (https=) |
| JP (1) | JP5787519B2 (https=) |
| CN (2) | CN107629109A (https=) |
| AU (1) | AU2008322473B2 (https=) |
| BR (1) | BRPI0820177B8 (https=) |
| CA (1) | CA2705765C (https=) |
| ES (1) | ES2456341T3 (https=) |
| WO (1) | WO2009065091A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US8962262B2 (en) * | 2006-05-11 | 2015-02-24 | Arbor Vita Corporation | Method of protein extraction from cells |
| US20100003704A1 (en) * | 2008-06-13 | 2010-01-07 | Shuling Cheng | IN SITU detection of early stages and late stages HPV infection |
| US9207240B2 (en) | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
| BR112012004699B1 (pt) | 2009-09-03 | 2019-10-15 | Becton, Dickinson And Company | Métodos, composições e kits para lise química direta |
| US8282895B2 (en) * | 2009-09-15 | 2012-10-09 | Qiagen Gaithersburg, Inc. | Reagent cabinet system |
| US8994937B2 (en) | 2012-04-18 | 2015-03-31 | Hewlett-Packard Development Company, L.P. | Surface enhanced Raman spectroscopy calibration curve generating systems |
| US9680916B2 (en) | 2013-08-01 | 2017-06-13 | Flowtraq, Inc. | Methods and systems for distribution and retrieval of network traffic records |
| JP6397224B2 (ja) * | 2014-06-04 | 2018-09-26 | 田中貴金属工業株式会社 | 免疫学的測定試薬におけるプロゾーン現象の解消法 |
| CN104237198A (zh) * | 2014-07-31 | 2014-12-24 | 中国人民解放军第二军医大学 | 一种判定中药材或中药饮片是否染色的方法 |
| EP3465217B1 (en) * | 2016-05-31 | 2025-09-03 | Roche Diagnostics GmbH | Method for serological detection of viral antigens |
| CN107621462B (zh) * | 2016-07-13 | 2020-03-31 | 王志伟 | 一种组织透明化液sut及其制备和应用 |
| WO2018130072A1 (zh) * | 2017-01-12 | 2018-07-19 | 厦门大学 | 稳定呼吸道合胞病毒融合蛋白的方法 |
| BR112020012490A2 (pt) * | 2017-12-22 | 2020-11-24 | Basf Plant Science Company Gmbh | método para a extração de proteínas ligadas à membrana e composição |
| JP7267548B2 (ja) * | 2019-04-24 | 2023-05-02 | 株式会社ニップン | トマト黄化葉巻ウイルス（ｔｙｌｃｖ）の免疫学的診断法 |
| CN112213164A (zh) * | 2019-07-12 | 2021-01-12 | 温州医科大学附属第一医院 | 一种总蛋白提取试剂盒及提取方法 |
| CN116223793A (zh) * | 2023-02-14 | 2023-06-06 | 上海乐备实生物技术有限公司 | 一种用于外泌体中补体检测的裂解液及其应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US3546334A (en) * | 1965-05-21 | 1970-12-08 | Lerner Lab Inc | Composition for fixing and protecting a smear of body cells and method of applying same |
| US3303038A (en) * | 1965-07-29 | 1967-02-07 | Ethicon Inc | Process of forming collagen articles and dispersions |
| JPS5535088B2 (https=) * | 1971-09-10 | 1980-09-11 | ||
| US4578282A (en) * | 1982-02-04 | 1986-03-25 | Harrison James S | Composition for diagnostic reagents |
| US4766065A (en) | 1984-08-23 | 1988-08-23 | Becton Dickinson And Company | Detection of cell membrane protein |
| US4649192A (en) * | 1985-05-30 | 1987-03-10 | Smith Kline-Rit | Method for the isolation and purification of hepatitis B surface antigen using polysorbate |
| US4857300A (en) * | 1987-07-27 | 1989-08-15 | Cytocorp, Inc. | Cytological and histological fixative formulation and methods for using same |
| US5132205A (en) | 1988-10-07 | 1992-07-21 | Eastman Kodak Company | High ph extraction composition and its use to determine a chlamydial, gonococcal or herpes antigen |
| US5047325A (en) | 1988-10-07 | 1991-09-10 | Eastman Kodak Company | Wash solution containing a cationic surfactant and its use in chlamydial and gonococcal determinations |
| US5081010A (en) | 1989-02-09 | 1992-01-14 | Eastman Kodak Company | Extraction composition, test kit and their use to extract or determine herpes simplex viral antigen |
| US5104640A (en) * | 1989-03-17 | 1992-04-14 | Wescor, Inc. | Fixative composition for fixing blood smears to slides |
| US5256571A (en) * | 1991-05-01 | 1993-10-26 | Cytyc Corporation | Cell preservative solution |
| US5196182A (en) * | 1991-05-08 | 1993-03-23 | Streck Laboratories, Inc. | Tissue fixative |
| US5773277A (en) * | 1992-06-26 | 1998-06-30 | Seikagaku Kogyo Kabushiki Kaisha | Crystalline chondroitinase isolated from Proteus vulgaris ATCC 6896 |
| US5432056A (en) * | 1993-11-15 | 1995-07-11 | Ventana Medical Systems, Inc. | Bisulfite-based tissue fixative |
| US5658749A (en) * | 1994-04-05 | 1997-08-19 | Corning Clinical Laboratories, Inc. | Method for processing mycobacteria |
| US5620869A (en) * | 1995-09-28 | 1997-04-15 | Becton, Dickinson And Company | Methods for reducing inhibition of nucleic acid amplification reactions |
| US7306926B2 (en) * | 1998-07-01 | 2007-12-11 | Mtm Laboratories Ag | Method for detecting carcinomas in a solubilized cervical body sample |
| US6245568B1 (en) | 1999-03-26 | 2001-06-12 | Merck & Co., Inc. | Human papilloma virus vaccine with disassembled and reassembled virus-like particles |
| US20050019841A1 (en) * | 1999-05-14 | 2005-01-27 | Arbor Vita Corporation | Modulation of signaling pathways |
| US20060148711A1 (en) * | 1999-05-14 | 2006-07-06 | Arbor Vita Corporation | Molecular interactions in neurons |
| US20050037969A1 (en) * | 1999-05-14 | 2005-02-17 | Arbor Vita Corporation | Molecular interactions in hematopoietic cells |
| US6488671B1 (en) * | 1999-10-22 | 2002-12-03 | Corazon Technologies, Inc. | Methods for enhancing fluid flow through an obstructed vascular site, and systems and kits for use in practicing the same |
| UA76708C2 (uk) * | 1999-12-08 | 2006-09-15 | Сінгента Патисипейшонс Аг | Антитіло, яке застосовується в імунологічному аналізі зразка для визначення неонікотиноїдного інсектициду, білковий кон'югат для одержання антитіла, спосіб визначення концентрації неонікотиноїдного інсектициду в зразку та набір для визначення кількості неонікотиноїдного інсектициду |
| US6337189B1 (en) * | 2000-02-08 | 2002-01-08 | Streck Laboratories, Inc. | Fixative system, method and composition for biological testing |
| US6436662B1 (en) * | 2000-04-04 | 2002-08-20 | Digene Corporation | Device and method for cytology slide preparation |
| WO2002007751A1 (en) * | 2000-07-25 | 2002-01-31 | Axcell Biosciences Corporation | Identification and isolation of novel polypeptides having pdz domains and methods of using same |
| US20030175852A1 (en) * | 2000-09-15 | 2003-09-18 | Kalra Krishan L | Ehancement of in situ hybridization |
| US7312041B2 (en) * | 2001-02-16 | 2007-12-25 | Arbor Vita Corporation | Methods of diagnosing cervical cancer |
| DK1402015T3 (da) | 2001-06-22 | 2011-12-05 | Hoffmann La Roche | Opløseligt kompleks omfattende et retroviralt overfladeglycoprotein og FkpA eller SlyD |
| US20050032105A1 (en) * | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| BR0314155A (pt) | 2002-09-09 | 2005-07-05 | Arbor Vita Corp | Métodos para diagnosticar câncer cervical |
| US7319021B2 (en) * | 2002-11-01 | 2008-01-15 | Promega Corporation | Cell lysis composition, methods of use, apparatus and kit |
| DE60300339T2 (de) | 2003-08-25 | 2006-04-13 | Mtm Laboratories Ag | Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben |
| DE602004026912D1 (de) * | 2003-12-16 | 2010-06-10 | Qiagen North American Holdings | Formulierungen und verfahren zum denaturieren von proteinen |
| JP5030594B2 (ja) * | 2003-12-23 | 2012-09-19 | アルボー ビータ コーポレーション | Ｈｐｖの発癌性株に対する抗体およびそれらの使用方法 |
| US7723044B2 (en) * | 2004-04-15 | 2010-05-25 | Allied Biotech, Inc. | Methods and apparatus for detection of viral infection |
| MXPA06014480A (es) * | 2004-06-09 | 2007-03-21 | Pathogen Removal And Diagnosti | Particulas incluidas en un substrato poroso para la remocion de analitos objetivo de una muestra. |
| US8962262B2 (en) * | 2006-05-11 | 2015-02-24 | Arbor Vita Corporation | Method of protein extraction from cells |
| US9207240B2 (en) | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
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2007
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- 2008-11-14 CN CN201710593190.6A patent/CN107629109A/zh active Pending
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- 2008-11-14 CN CN200880124680.0A patent/CN101910400B/zh not_active Expired - Fee Related
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2015
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Also Published As
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| EP2222838A4 (en) | 2011-01-05 |
| ES2456341T3 (es) | 2014-04-22 |
| US9207240B2 (en) | 2015-12-08 |
| BRPI0820177A2 (pt) | 2014-11-04 |
| US9995745B2 (en) | 2018-06-12 |
| US20160216258A1 (en) | 2016-07-28 |
| JP5787519B2 (ja) | 2015-09-30 |
| JP2011503208A (ja) | 2011-01-27 |
| CN101910400B (zh) | 2017-06-27 |
| CA2705765A1 (en) | 2009-05-22 |
| AU2008322473A1 (en) | 2009-05-22 |
| BRPI0820177B8 (pt) | 2021-07-27 |
| US20090123910A1 (en) | 2009-05-14 |
| CN107629109A (zh) | 2018-01-26 |
| AU2008322473B2 (en) | 2014-07-03 |
| WO2009065091A1 (en) | 2009-05-22 |
| EP2222838A1 (en) | 2010-09-01 |
| EP2222838B1 (en) | 2014-01-22 |
| BRPI0820177B1 (pt) | 2019-07-02 |
| CN101910400A (zh) | 2010-12-08 |
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