JP5787519B2 - 細胞からの効率的なタンパク質抽出方法 - Google Patents

細胞からの効率的なタンパク質抽出方法 Download PDF

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JP5787519B2
JP5787519B2 JP2010534253A JP2010534253A JP5787519B2 JP 5787519 B2 JP5787519 B2 JP 5787519B2 JP 2010534253 A JP2010534253 A JP 2010534253A JP 2010534253 A JP2010534253 A JP 2010534253A JP 5787519 B2 JP5787519 B2 JP 5787519B2
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protein
brij
hpv
cells
reagent
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JP2011503208A (ja
JP2011503208A5 (https=
Inventor
エイドリアン ピー． マリック
エイドリアン ピー． マリック
バージニア エム． クルース
バージニア エム． クルース
ジュリー エル． ロサレス
ジュリー エル． ロサレス
キャリー エス． ファーガスン
キャリー エス． ファーガスン
ジェフェリー エイチ． ブルートン
ジェフェリー エイチ． ブルートン
ロバート ジェイ． ビーデンコフ
ロバート ジェイ． ビーデンコフ
ジョン マントロ
ジョン マントロ
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Becton Dickinson and Co
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/10—Detection of antigens from microorganism in sample from host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
JP2010534253A 2007-11-14 2008-11-14 細胞からの効率的なタンパク質抽出方法 Expired - Fee Related JP5787519B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/985,547 2007-11-14
US11/985,547 US9207240B2 (en) 2007-11-14 2007-11-14 Method of efficient extraction of protein from cells
PCT/US2008/083707 WO2009065091A1 (en) 2007-11-14 2008-11-14 Method of efficient extraction of protein from cells

Publications (3)

Publication Number Publication Date
JP2011503208A JP2011503208A (ja) 2011-01-27
JP2011503208A5 JP2011503208A5 (https=) 2012-01-05
JP5787519B2 true JP5787519B2 (ja) 2015-09-30

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JP2010534253A Expired - Fee Related JP5787519B2 (ja) 2007-11-14 2008-11-14 細胞からの効率的なタンパク質抽出方法

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Country Link
US (2) US9207240B2 (https=)
EP (1) EP2222838B1 (https=)
JP (1) JP5787519B2 (https=)
CN (2) CN107629109A (https=)
AU (1) AU2008322473B2 (https=)
BR (1) BRPI0820177B8 (https=)
CA (1) CA2705765C (https=)
ES (1) ES2456341T3 (https=)
WO (1) WO2009065091A1 (https=)

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JP6397224B2 (ja) * 2014-06-04 2018-09-26 田中貴金属工業株式会社 免疫学的測定試薬におけるプロゾーン現象の解消法
CN104237198A (zh) * 2014-07-31 2014-12-24 中国人民解放军第二军医大学 一种判定中药材或中药饮片是否染色的方法
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CN107621462B (zh) * 2016-07-13 2020-03-31 王志伟 一种组织透明化液sut及其制备和应用
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JP7267548B2 (ja) * 2019-04-24 2023-05-02 株式会社ニップン トマト黄化葉巻ウイルス（ｔｙｌｃｖ）の免疫学的診断法
CN112213164A (zh) * 2019-07-12 2021-01-12 温州医科大学附属第一医院 一种总蛋白提取试剂盒及提取方法
CN116223793A (zh) * 2023-02-14 2023-06-06 上海乐备实生物技术有限公司 一种用于外泌体中补体检测的裂解液及其应用

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Also Published As

Publication number Publication date
EP2222838A4 (en) 2011-01-05
ES2456341T3 (es) 2014-04-22
US9207240B2 (en) 2015-12-08
BRPI0820177A2 (pt) 2014-11-04
US9995745B2 (en) 2018-06-12
US20160216258A1 (en) 2016-07-28
JP2011503208A (ja) 2011-01-27
CN101910400B (zh) 2017-06-27
CA2705765A1 (en) 2009-05-22
AU2008322473A1 (en) 2009-05-22
BRPI0820177B8 (pt) 2021-07-27
US20090123910A1 (en) 2009-05-14
CN107629109A (zh) 2018-01-26
AU2008322473B2 (en) 2014-07-03
WO2009065091A1 (en) 2009-05-22
EP2222838A1 (en) 2010-09-01
CA2705765C (en) 2018-12-11
EP2222838B1 (en) 2014-01-22
BRPI0820177B1 (pt) 2019-07-02
CN101910400A (zh) 2010-12-08

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