JP2009536958A5 - - Google Patents
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- JP2009536958A5 JP2009536958A5 JP2009510190A JP2009510190A JP2009536958A5 JP 2009536958 A5 JP2009536958 A5 JP 2009536958A5 JP 2009510190 A JP2009510190 A JP 2009510190A JP 2009510190 A JP2009510190 A JP 2009510190A JP 2009536958 A5 JP2009536958 A5 JP 2009536958A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- reagent
- hpv
- intermediate composition
- fixed cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 claims description 43
- 239000002736 nonionic surfactant Substances 0.000 claims description 8
- 239000004094 surface-active agent Substances 0.000 claims description 7
- 238000006386 neutralization reaction Methods 0.000 claims description 4
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims description 3
- 229920000136 polysorbate Polymers 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 27
- 108090000623 proteins and genes Proteins 0.000 claims 27
- 102000004169 proteins and genes Human genes 0.000 claims 27
- 241000701806 Human papillomavirus Species 0.000 claims 13
- 239000000203 mixture Substances 0.000 claims 12
- 238000000605 extraction Methods 0.000 claims 11
- 230000003472 neutralizing effect Effects 0.000 claims 10
- 108010067390 Viral Proteins Proteins 0.000 claims 9
- 239000003795 chemical substances by application Substances 0.000 claims 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 3
- 102000000470 PDZ domains Human genes 0.000 claims 3
- 108050008994 PDZ domains Proteins 0.000 claims 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims 3
- 241000700605 Viruses Species 0.000 claims 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical group CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims 2
- 238000002965 ELISA Methods 0.000 claims 2
- 241000725643 Respiratory syncytial virus Species 0.000 claims 2
- 241000700584 Simplexvirus Species 0.000 claims 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 2
- 150000001299 aldehydes Chemical class 0.000 claims 2
- 239000000834 fixative Substances 0.000 claims 2
- 150000002576 ketones Chemical class 0.000 claims 2
- 230000001717 pathogenic effect Effects 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 101100489349 Arabidopsis thaliana PAT24 gene Proteins 0.000 claims 1
- 241001115402 Ebolavirus Species 0.000 claims 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims 1
- 101100315589 Homo sapiens TAX1BP3 gene Proteins 0.000 claims 1
- 241000341655 Human papillomavirus type 16 Species 0.000 claims 1
- 241001115401 Marburgvirus Species 0.000 claims 1
- 102100027240 Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 Human genes 0.000 claims 1
- 101710137278 Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 Proteins 0.000 claims 1
- 101100313925 Oryza sativa subsp. japonica TIP1-2 gene Proteins 0.000 claims 1
- 101100313932 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TIP20 gene Proteins 0.000 claims 1
- 241000580858 Simian-Human immunodeficiency virus Species 0.000 claims 1
- 102100036221 Tax1-binding protein 3 Human genes 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 101100313921 Zea mays TIP1-1 gene Proteins 0.000 claims 1
- 150000001298 alcohols Chemical class 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 229910001385 heavy metal Inorganic materials 0.000 claims 1
- 208000006454 hepatitis Diseases 0.000 claims 1
- 231100000283 hepatitis Toxicity 0.000 claims 1
- 238000003018 immunoassay Methods 0.000 claims 1
- -1 ion salts Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims 1
- 210000004877 mucosa Anatomy 0.000 claims 1
- 231100000590 oncogenic Toxicity 0.000 claims 1
- 230000002246 oncogenic effect Effects 0.000 claims 1
- 239000012285 osmium tetroxide Substances 0.000 claims 1
- 229910000489 osmium tetroxide Inorganic materials 0.000 claims 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 238000000159 protein binding assay Methods 0.000 claims 1
- 229940016590 sarkosyl Drugs 0.000 claims 1
- 108700004121 sarkosyl Proteins 0.000 claims 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 claims 1
- 101150023068 tip1 gene Proteins 0.000 claims 1
- 239000000872 buffer Substances 0.000 description 2
- 239000011536 extraction buffer Substances 0.000 description 2
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74707606P | 2006-05-11 | 2006-05-11 | |
| PCT/US2007/068809 WO2007134252A1 (en) | 2006-05-11 | 2007-05-11 | Method of protein extraction from cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015237245A Division JP6877871B2 (ja) | 2006-05-11 | 2015-12-04 | 細胞からのタンパク質抽出方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2009536958A JP2009536958A (ja) | 2009-10-22 |
| JP2009536958A5 true JP2009536958A5 (https=) | 2010-05-27 |
Family
ID=38694244
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009510190A Withdrawn JP2009536958A (ja) | 2006-05-11 | 2007-05-11 | 細胞からのタンパク質抽出方法 |
| JP2015237245A Active JP6877871B2 (ja) | 2006-05-11 | 2015-12-04 | 細胞からのタンパク質抽出方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015237245A Active JP6877871B2 (ja) | 2006-05-11 | 2015-12-04 | 細胞からのタンパク質抽出方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US8962262B2 (https=) |
| EP (1) | EP2024520B1 (https=) |
| JP (2) | JP2009536958A (https=) |
| CN (2) | CN104497098A (https=) |
| AU (1) | AU2007249242B2 (https=) |
| CA (1) | CA2652118C (https=) |
| ES (1) | ES2462040T3 (https=) |
| WO (1) | WO2007134252A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| JP2009536958A (ja) | 2006-05-11 | 2009-10-22 | ベクトン・ディキンソン・アンド・カンパニー | 細胞からのタンパク質抽出方法 |
| US9207240B2 (en) * | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
| TWI419974B (zh) | 2008-10-27 | 2013-12-21 | Qiagen Gaithersburg Inc | 於自動平台上之快速結果雜交捕捉法 |
| ES2644516T3 (es) * | 2009-01-28 | 2017-11-29 | Qiagen Gaithersburg, Inc. | Método y ensayo de preparación de muestras de gran volumen específico de secuencia |
| WO2010127324A2 (en) * | 2009-04-30 | 2010-11-04 | Arbor Vita Corporation | Chimeric pdz domain-containing protein for viral detection |
| WO2010127326A1 (en) * | 2009-04-30 | 2010-11-04 | Becton, Dickinson And Company | E6 binding peptides for use in affinity purification |
| WO2010127228A1 (en) | 2009-05-01 | 2010-11-04 | Qiagen Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
| JP2013517803A (ja) | 2010-01-29 | 2013-05-20 | キアジェン ゲイサーズバーグ インコーポレイテッド | サンプル中のｈｐｖの存在を決定および確認する方法 |
| CA2787924A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| EP2572001A2 (en) | 2010-05-19 | 2013-03-27 | QIAGEN Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー． | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
| WO2012116220A2 (en) | 2011-02-24 | 2012-08-30 | Qiagen Gaithersburg, Inc. | Materials and methods for detection of hpv nucleic acid |
| US10254279B2 (en) | 2013-03-29 | 2019-04-09 | Nima Labs, Inc. | System and method for detection of target substances |
| US10466236B2 (en) | 2013-03-29 | 2019-11-05 | Nima Labs, Inc. | System and method for detecting target substances |
| CN105209912B (zh) | 2013-03-29 | 2018-04-17 | 第6感传感器实验室公司 | 用于有害物质的检测的便携式装置 |
| CN103461320B (zh) * | 2013-08-19 | 2015-09-02 | 安徽信灵检验医学科技有限公司 | 一种用于液基薄层脱落细胞的不等张保存液 |
| JP2019523863A (ja) * | 2016-05-31 | 2019-08-29 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ウイルス抗原の血清学的検出方法 |
Family Cites Families (38)
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|---|---|---|---|---|
| US3546334A (en) * | 1965-05-21 | 1970-12-08 | Lerner Lab Inc | Composition for fixing and protecting a smear of body cells and method of applying same |
| US3303038A (en) * | 1965-07-29 | 1967-02-07 | Ethicon Inc | Process of forming collagen articles and dispersions |
| JPS5535088B2 (https=) * | 1971-09-10 | 1980-09-11 | ||
| US4578282A (en) * | 1982-02-04 | 1986-03-25 | Harrison James S | Composition for diagnostic reagents |
| US4766065A (en) | 1984-08-23 | 1988-08-23 | Becton Dickinson And Company | Detection of cell membrane protein |
| US4649192A (en) * | 1985-05-30 | 1987-03-10 | Smith Kline-Rit | Method for the isolation and purification of hepatitis B surface antigen using polysorbate |
| US4857300A (en) * | 1987-07-27 | 1989-08-15 | Cytocorp, Inc. | Cytological and histological fixative formulation and methods for using same |
| US5047325A (en) * | 1988-10-07 | 1991-09-10 | Eastman Kodak Company | Wash solution containing a cationic surfactant and its use in chlamydial and gonococcal determinations |
| US5132205A (en) | 1988-10-07 | 1992-07-21 | Eastman Kodak Company | High ph extraction composition and its use to determine a chlamydial, gonococcal or herpes antigen |
| US5081010A (en) | 1989-02-09 | 1992-01-14 | Eastman Kodak Company | Extraction composition, test kit and their use to extract or determine herpes simplex viral antigen |
| US5104640A (en) * | 1989-03-17 | 1992-04-14 | Wescor, Inc. | Fixative composition for fixing blood smears to slides |
| US5256571A (en) * | 1991-05-01 | 1993-10-26 | Cytyc Corporation | Cell preservative solution |
| US5196182A (en) * | 1991-05-08 | 1993-03-23 | Streck Laboratories, Inc. | Tissue fixative |
| US5773277A (en) * | 1992-06-26 | 1998-06-30 | Seikagaku Kogyo Kabushiki Kaisha | Crystalline chondroitinase isolated from Proteus vulgaris ATCC 6896 |
| US5432056A (en) * | 1993-11-15 | 1995-07-11 | Ventana Medical Systems, Inc. | Bisulfite-based tissue fixative |
| US5658749A (en) * | 1994-04-05 | 1997-08-19 | Corning Clinical Laboratories, Inc. | Method for processing mycobacteria |
| US5620869A (en) * | 1995-09-28 | 1997-04-15 | Becton, Dickinson And Company | Methods for reducing inhibition of nucleic acid amplification reactions |
| US7306926B2 (en) * | 1998-07-01 | 2007-12-11 | Mtm Laboratories Ag | Method for detecting carcinomas in a solubilized cervical body sample |
| US6245568B1 (en) | 1999-03-26 | 2001-06-12 | Merck & Co., Inc. | Human papilloma virus vaccine with disassembled and reassembled virus-like particles |
| US20060148711A1 (en) * | 1999-05-14 | 2006-07-06 | Arbor Vita Corporation | Molecular interactions in neurons |
| US20050037969A1 (en) * | 1999-05-14 | 2005-02-17 | Arbor Vita Corporation | Molecular interactions in hematopoietic cells |
| US20050019841A1 (en) * | 1999-05-14 | 2005-01-27 | Arbor Vita Corporation | Modulation of signaling pathways |
| US6488671B1 (en) * | 1999-10-22 | 2002-12-03 | Corazon Technologies, Inc. | Methods for enhancing fluid flow through an obstructed vascular site, and systems and kits for use in practicing the same |
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| US6436662B1 (en) | 2000-04-04 | 2002-08-20 | Digene Corporation | Device and method for cytology slide preparation |
| AU2001277986A1 (en) * | 2000-07-25 | 2002-02-05 | Axcell Biosciences Corporation | Identification and isolation of novel polypeptides having pdz domains and methods of using same |
| JP2004515752A (ja) * | 2000-09-15 | 2004-05-27 | バイオジェネックス ラボラトリーズ | ｉｎｓｉｔｕハイブリダイゼーションの向上 |
| US7312041B2 (en) * | 2001-02-16 | 2007-12-25 | Arbor Vita Corporation | Methods of diagnosing cervical cancer |
| MXPA03011455A (es) | 2001-06-22 | 2004-04-05 | Hoffmann La Roche | Complejo soluble que comprende glucoproteina de superficie retroviral. |
| US20050032105A1 (en) * | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| JP4694840B2 (ja) * | 2002-09-09 | 2011-06-08 | アルボー ビータ コーポレーション | 子宮頸部癌を検査する方法 |
| WO2004042003A2 (en) * | 2002-11-01 | 2004-05-21 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
| ATE289685T1 (de) | 2003-08-25 | 2005-03-15 | Mtm Lab Ag | Verfahren zum nachweis von karzinomen in solubilisierten zervikalen körperproben |
| DE602004026912D1 (de) * | 2003-12-16 | 2010-06-10 | Qiagen North American Holdings | Formulierungen und verfahren zum denaturieren von proteinen |
| WO2005063286A1 (en) * | 2003-12-23 | 2005-07-14 | Arbor Vita Corporation | Antibodies for oncogenic strains of hpv and methods of their use |
| AU2005250325A1 (en) | 2004-04-15 | 2005-12-15 | Allied Biotech, Inc. | Methods and apparatus for detection of viral infection |
| JP2009536958A (ja) | 2006-05-11 | 2009-10-22 | ベクトン・ディキンソン・アンド・カンパニー | 細胞からのタンパク質抽出方法 |
| US9207240B2 (en) * | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
-
2007
- 2007-05-11 JP JP2009510190A patent/JP2009536958A/ja not_active Withdrawn
- 2007-05-11 CN CN201410545307.XA patent/CN104497098A/zh active Pending
- 2007-05-11 ES ES07797439.2T patent/ES2462040T3/es active Active
- 2007-05-11 EP EP07797439.2A patent/EP2024520B1/en active Active
- 2007-05-11 AU AU2007249242A patent/AU2007249242B2/en active Active
- 2007-05-11 WO PCT/US2007/068809 patent/WO2007134252A1/en not_active Ceased
- 2007-05-11 CA CA2652118A patent/CA2652118C/en active Active
- 2007-05-11 US US11/747,830 patent/US8962262B2/en active Active
- 2007-05-11 CN CNA2007800210313A patent/CN101460636A/zh active Pending
-
2015
- 2015-01-15 US US14/598,148 patent/US9771398B2/en active Active
- 2015-12-04 JP JP2015237245A patent/JP6877871B2/ja active Active
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