CA2652118C - Method of protein extraction from cells - Google Patents
Method of protein extraction from cells Download PDFInfo
- Publication number
- CA2652118C CA2652118C CA2652118A CA2652118A CA2652118C CA 2652118 C CA2652118 C CA 2652118C CA 2652118 A CA2652118 A CA 2652118A CA 2652118 A CA2652118 A CA 2652118A CA 2652118 C CA2652118 C CA 2652118C
- Authority
- CA
- Canada
- Prior art keywords
- hpv
- protein
- cells
- reagent
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/82—Translation products from oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20051—Methods of production or purification of viral material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
- G01N2333/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Oncology (AREA)
- General Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74707606P | 2006-05-11 | 2006-05-11 | |
| US60/747,076 | 2006-05-11 | ||
| PCT/US2007/068809 WO2007134252A1 (en) | 2006-05-11 | 2007-05-11 | Method of protein extraction from cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2652118A1 CA2652118A1 (en) | 2007-11-22 |
| CA2652118C true CA2652118C (en) | 2015-07-07 |
Family
ID=38694244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2652118A Active CA2652118C (en) | 2006-05-11 | 2007-05-11 | Method of protein extraction from cells |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US8962262B2 (https=) |
| EP (1) | EP2024520B1 (https=) |
| JP (2) | JP2009536958A (https=) |
| CN (2) | CN104497098A (https=) |
| AU (1) | AU2007249242B2 (https=) |
| CA (1) | CA2652118C (https=) |
| ES (1) | ES2462040T3 (https=) |
| WO (1) | WO2007134252A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US8962262B2 (en) | 2006-05-11 | 2015-02-24 | Arbor Vita Corporation | Method of protein extraction from cells |
| US9207240B2 (en) | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
| WO2010062546A1 (en) | 2008-10-27 | 2010-06-03 | Qiagen Gaithersburg Inc. | Fast results hybrid capture assay on an automated platform |
| JP6108661B2 (ja) * | 2009-01-28 | 2017-04-05 | キアジェン ゲイサーズバーグ インコーポレイテッド | 配列特異的な大量試料調製方法およびアッセイ法 |
| WO2010127324A2 (en) * | 2009-04-30 | 2010-11-04 | Arbor Vita Corporation | Chimeric pdz domain-containing protein for viral detection |
| WO2010127326A1 (en) * | 2009-04-30 | 2010-11-04 | Becton, Dickinson And Company | E6 binding peptides for use in affinity purification |
| EP2425019B1 (en) * | 2009-05-01 | 2014-03-19 | QIAGEN Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
| CA2787781A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Method of determining and confirming the presence of an hpv in a sample |
| CA2787924A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP2013528049A (ja) | 2010-05-19 | 2013-07-08 | キアゲン ガイサーズバーグ アイエヌシー. | 核酸の配列特異的な精製及び多重分析のための方法及び組成物 |
| JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー. | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
| CN103597095B (zh) | 2011-02-24 | 2016-08-17 | 奇亚根盖瑟斯堡股份有限公司 | 用于检测hpv核酸的材料和方法 |
| EP2979092B1 (en) | 2013-03-29 | 2018-05-30 | Nima Labs, Inc. | A portable device for detection of harmful substances |
| US10466236B2 (en) | 2013-03-29 | 2019-11-05 | Nima Labs, Inc. | System and method for detecting target substances |
| US10254279B2 (en) | 2013-03-29 | 2019-04-09 | Nima Labs, Inc. | System and method for detection of target substances |
| CN103461320B (zh) * | 2013-08-19 | 2015-09-02 | 安徽信灵检验医学科技有限公司 | 一种用于液基薄层脱落细胞的不等张保存液 |
| EP3465217B1 (en) * | 2016-05-31 | 2025-09-03 | Roche Diagnostics GmbH | Method for serological detection of viral antigens |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3546334A (en) * | 1965-05-21 | 1970-12-08 | Lerner Lab Inc | Composition for fixing and protecting a smear of body cells and method of applying same |
| US3303038A (en) * | 1965-07-29 | 1967-02-07 | Ethicon Inc | Process of forming collagen articles and dispersions |
| JPS5535088B2 (https=) * | 1971-09-10 | 1980-09-11 | ||
| US4578282A (en) * | 1982-02-04 | 1986-03-25 | Harrison James S | Composition for diagnostic reagents |
| US4766065A (en) * | 1984-08-23 | 1988-08-23 | Becton Dickinson And Company | Detection of cell membrane protein |
| US4649192A (en) * | 1985-05-30 | 1987-03-10 | Smith Kline-Rit | Method for the isolation and purification of hepatitis B surface antigen using polysorbate |
| US4857300A (en) * | 1987-07-27 | 1989-08-15 | Cytocorp, Inc. | Cytological and histological fixative formulation and methods for using same |
| US5047325A (en) * | 1988-10-07 | 1991-09-10 | Eastman Kodak Company | Wash solution containing a cationic surfactant and its use in chlamydial and gonococcal determinations |
| US5132205A (en) * | 1988-10-07 | 1992-07-21 | Eastman Kodak Company | High ph extraction composition and its use to determine a chlamydial, gonococcal or herpes antigen |
| US5081010A (en) | 1989-02-09 | 1992-01-14 | Eastman Kodak Company | Extraction composition, test kit and their use to extract or determine herpes simplex viral antigen |
| US5104640A (en) * | 1989-03-17 | 1992-04-14 | Wescor, Inc. | Fixative composition for fixing blood smears to slides |
| US5256571A (en) * | 1991-05-01 | 1993-10-26 | Cytyc Corporation | Cell preservative solution |
| US5196182A (en) * | 1991-05-08 | 1993-03-23 | Streck Laboratories, Inc. | Tissue fixative |
| US5773277A (en) * | 1992-06-26 | 1998-06-30 | Seikagaku Kogyo Kabushiki Kaisha | Crystalline chondroitinase isolated from Proteus vulgaris ATCC 6896 |
| US5432056A (en) * | 1993-11-15 | 1995-07-11 | Ventana Medical Systems, Inc. | Bisulfite-based tissue fixative |
| US5658749A (en) * | 1994-04-05 | 1997-08-19 | Corning Clinical Laboratories, Inc. | Method for processing mycobacteria |
| US5620869A (en) * | 1995-09-28 | 1997-04-15 | Becton, Dickinson And Company | Methods for reducing inhibition of nucleic acid amplification reactions |
| US7306926B2 (en) * | 1998-07-01 | 2007-12-11 | Mtm Laboratories Ag | Method for detecting carcinomas in a solubilized cervical body sample |
| US6245568B1 (en) * | 1999-03-26 | 2001-06-12 | Merck & Co., Inc. | Human papilloma virus vaccine with disassembled and reassembled virus-like particles |
| US20050019841A1 (en) * | 1999-05-14 | 2005-01-27 | Arbor Vita Corporation | Modulation of signaling pathways |
| US20060148711A1 (en) * | 1999-05-14 | 2006-07-06 | Arbor Vita Corporation | Molecular interactions in neurons |
| US20050037969A1 (en) * | 1999-05-14 | 2005-02-17 | Arbor Vita Corporation | Molecular interactions in hematopoietic cells |
| US6488671B1 (en) * | 1999-10-22 | 2002-12-03 | Corazon Technologies, Inc. | Methods for enhancing fluid flow through an obstructed vascular site, and systems and kits for use in practicing the same |
| US6337189B1 (en) * | 2000-02-08 | 2002-01-08 | Streck Laboratories, Inc. | Fixative system, method and composition for biological testing |
| US6436662B1 (en) | 2000-04-04 | 2002-08-20 | Digene Corporation | Device and method for cytology slide preparation |
| WO2002007751A1 (en) * | 2000-07-25 | 2002-01-31 | Axcell Biosciences Corporation | Identification and isolation of novel polypeptides having pdz domains and methods of using same |
| US20030175852A1 (en) * | 2000-09-15 | 2003-09-18 | Kalra Krishan L | Ehancement of in situ hybridization |
| US7312041B2 (en) * | 2001-02-16 | 2007-12-25 | Arbor Vita Corporation | Methods of diagnosing cervical cancer |
| DK1402015T3 (da) | 2001-06-22 | 2011-12-05 | Hoffmann La Roche | Opløseligt kompleks omfattende et retroviralt overfladeglycoprotein og FkpA eller SlyD |
| US20050032105A1 (en) | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| BR0314155A (pt) | 2002-09-09 | 2005-07-05 | Arbor Vita Corp | Métodos para diagnosticar câncer cervical |
| US7319021B2 (en) * | 2002-11-01 | 2008-01-15 | Promega Corporation | Cell lysis composition, methods of use, apparatus and kit |
| DE60300339T2 (de) * | 2003-08-25 | 2006-04-13 | Mtm Laboratories Ag | Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben |
| DE602004026912D1 (de) * | 2003-12-16 | 2010-06-10 | Qiagen North American Holdings | Formulierungen und verfahren zum denaturieren von proteinen |
| JP5030594B2 (ja) * | 2003-12-23 | 2012-09-19 | アルボー ビータ コーポレーション | Hpvの発癌性株に対する抗体およびそれらの使用方法 |
| US7723044B2 (en) | 2004-04-15 | 2010-05-25 | Allied Biotech, Inc. | Methods and apparatus for detection of viral infection |
| US8962262B2 (en) | 2006-05-11 | 2015-02-24 | Arbor Vita Corporation | Method of protein extraction from cells |
| US9207240B2 (en) * | 2007-11-14 | 2015-12-08 | Arbor Vita Corporation | Method of efficient extraction of protein from cells |
-
2007
- 2007-05-11 US US11/747,830 patent/US8962262B2/en active Active
- 2007-05-11 WO PCT/US2007/068809 patent/WO2007134252A1/en not_active Ceased
- 2007-05-11 CN CN201410545307.XA patent/CN104497098A/zh active Pending
- 2007-05-11 ES ES07797439.2T patent/ES2462040T3/es active Active
- 2007-05-11 CA CA2652118A patent/CA2652118C/en active Active
- 2007-05-11 JP JP2009510190A patent/JP2009536958A/ja not_active Withdrawn
- 2007-05-11 AU AU2007249242A patent/AU2007249242B2/en active Active
- 2007-05-11 CN CNA2007800210313A patent/CN101460636A/zh active Pending
- 2007-05-11 EP EP07797439.2A patent/EP2024520B1/en active Active
-
2015
- 2015-01-15 US US14/598,148 patent/US9771398B2/en active Active
- 2015-12-04 JP JP2015237245A patent/JP6877871B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2024520B1 (en) | 2014-03-05 |
| EP2024520A4 (en) | 2011-01-05 |
| US9771398B2 (en) | 2017-09-26 |
| WO2007134252A1 (en) | 2007-11-22 |
| EP2024520A1 (en) | 2009-02-18 |
| ES2462040T3 (es) | 2014-05-22 |
| CN101460636A (zh) | 2009-06-17 |
| JP2009536958A (ja) | 2009-10-22 |
| JP6877871B2 (ja) | 2021-05-26 |
| CN104497098A (zh) | 2015-04-08 |
| CA2652118A1 (en) | 2007-11-22 |
| AU2007249242B2 (en) | 2014-02-27 |
| AU2007249242A1 (en) | 2007-11-22 |
| US20070292899A1 (en) | 2007-12-20 |
| JP2016104741A (ja) | 2016-06-09 |
| US8962262B2 (en) | 2015-02-24 |
| US20150266928A1 (en) | 2015-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2652118C (en) | Method of protein extraction from cells | |
| US9995745B2 (en) | Method of efficient extraction of protein from cells | |
| EP2300824B1 (en) | Detection of early stages and late stages hpv infection | |
| US9249212B2 (en) | Anti-HPV E7 antibodies | |
| JP5819851B2 (ja) | Hpv関連の癌の治療及びスクリーニングのための細胞に基づいた高処理能力hpv免疫アッセイ | |
| WO2016015059A1 (en) | Systems and methods for early detection of cervical cancer by multiplex protein biomarkers | |
| CN104004067A (zh) | 用于检测抗人乳头瘤病毒抗体的抗原及相关免疫检测试剂盒 | |
| Jeon et al. | Immunocytochemical detection of HPV16 E7 in cervical smear | |
| BRPI0805220A2 (pt) | métodos para produzir um extrato de proteìna a partir de células fixas e para detectar uma proteìna, sistema e kit para produzir um extrato de proteìna, e, método para extrair uma proteìna viral alvo de uma amostra de célula | |
| US20240410890A1 (en) | Screening systems and methods for hpv-associated cervical disease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MPN | Maintenance fee for patent paid |
Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 18TH ANNIV.) - STANDARD Year of fee payment: 18 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250424 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250424 |