JP2010539991A - 電子顕微鏡を用いた核酸ポリマーの配列決定 - Google Patents
電子顕微鏡を用いた核酸ポリマーの配列決定 Download PDFInfo
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013069773A1 (ja) * | 2011-11-11 | 2013-05-16 | ナガヤマ アイピー ホールディングス エルエルシー | 塩基対応標識方法、塩基配列情報取得方法及び塩基対応標識化一本鎖核酸 |
| JP2015514653A (ja) * | 2012-01-19 | 2015-05-21 | ジェームズ ダーリング、マイケル | グラフェンナノ構造のdna画定エッチング法 |
| JP2018141782A (ja) * | 2017-02-27 | 2018-09-13 | 住友金属鉱山株式会社 | 汚染堆積物の定量方法および試料分析方法 |
Families Citing this family (106)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| US7888127B2 (en) | 2008-01-15 | 2011-02-15 | Sequenom, Inc. | Methods for reducing adduct formation for mass spectrometry analysis |
| EP3216874A1 (en) | 2008-09-05 | 2017-09-13 | TOMA Biosciences, Inc. | Methods for stratifying and annotating cancer drug treatment options |
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| US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
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| US9260745B2 (en) | 2010-01-19 | 2016-02-16 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| US20120010085A1 (en) | 2010-01-19 | 2012-01-12 | Rava Richard P | Methods for determining fraction of fetal nucleic acids in maternal samples |
| US20120100548A1 (en) | 2010-10-26 | 2012-04-26 | Verinata Health, Inc. | Method for determining copy number variations |
| ES2534986T3 (es) | 2010-01-19 | 2015-05-04 | Verinata Health, Inc | Determinación simultánea de aneuploidía y fracción fetal |
| EP2526415B1 (en) | 2010-01-19 | 2017-05-03 | Verinata Health, Inc | Partition defined detection methods |
| US9323888B2 (en) | 2010-01-19 | 2016-04-26 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| EP2534667A2 (en) | 2010-02-10 | 2012-12-19 | Mochii, Inc. (d/b/a Voxa) | Aberration-correcting dark-field electron microscopy |
| KR20130113447A (ko) | 2010-09-24 | 2013-10-15 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화 |
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| WO2012125547A2 (en) * | 2011-03-11 | 2012-09-20 | Cornell University | Systems and methods for high resolution biomolecular imaging and analysis |
| US20150065358A1 (en) | 2011-03-30 | 2015-03-05 | Verinata Health, Inc. | Method for verifying bioassay samples |
| SMT202200090T1 (it) | 2011-04-12 | 2022-03-21 | Verinata Health Inc | Risoluzione di frazioni genomiche usando conteggi di polimorfismo |
| WO2012141712A1 (en) | 2011-04-14 | 2012-10-18 | Verinata Health, Inc. | Normalizing chromosomes for the determination and verification of common and rare chromosomal aneuploidies |
| US9411937B2 (en) | 2011-04-15 | 2016-08-09 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| WO2012177792A2 (en) | 2011-06-24 | 2012-12-27 | Sequenom, Inc. | Methods and processes for non-invasive assessment of a genetic variation |
| US20130029348A1 (en) * | 2011-07-26 | 2013-01-31 | Opgen, Inc. | Methods of elongating nucleic acids |
| EP2563937A1 (en) | 2011-07-26 | 2013-03-06 | Verinata Health, Inc | Method for determining the presence or absence of different aneuploidies in a sample |
| CA2850785C (en) | 2011-10-06 | 2022-12-13 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10424394B2 (en) | 2011-10-06 | 2019-09-24 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9984198B2 (en) | 2011-10-06 | 2018-05-29 | Sequenom, Inc. | Reducing sequence read count error in assessment of complex genetic variations |
| ES2886508T3 (es) | 2011-10-06 | 2021-12-20 | Sequenom Inc | Métodos y procedimientos para la evaluación no invasiva de variaciones genéticas |
| US9367663B2 (en) | 2011-10-06 | 2016-06-14 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10196681B2 (en) | 2011-10-06 | 2019-02-05 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US8688388B2 (en) | 2011-10-11 | 2014-04-01 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| EP2766496B1 (en) | 2011-10-11 | 2017-03-08 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| EP2766498B1 (en) | 2011-10-14 | 2019-06-19 | President and Fellows of Harvard College | Sequencing by structure assembly |
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| WO2013073610A1 (ja) * | 2011-11-15 | 2013-05-23 | ナガヤマ アイピー ホールディングス エルエルシー | 塩基配列決定装置 |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP4249605B1 (en) | 2011-12-22 | 2024-08-28 | President And Fellows Of Harvard College | Methods for analyte detection |
| JP6431769B2 (ja) | 2012-01-20 | 2018-11-28 | セクエノム, インコーポレイテッド | 実験条件を要因として含める診断プロセス |
| US9856513B2 (en) * | 2012-01-27 | 2018-01-02 | Cornell University | Methods and arrays for controlled manipulation of DNA and chromatin fragments for genetic and epigenetic analysis |
| EP3401399B1 (en) | 2012-03-02 | 2020-04-22 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| US10497461B2 (en) | 2012-06-22 | 2019-12-03 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| HK1206792A1 (en) | 2012-07-13 | 2016-01-15 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| AU2013204615A1 (en) | 2012-07-20 | 2014-02-06 | Verinata Health, Inc. | Detecting and classifying copy number variation in a fetal genome |
| CA3120521A1 (en) | 2012-10-04 | 2014-04-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10482994B2 (en) | 2012-10-04 | 2019-11-19 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US20130309666A1 (en) | 2013-01-25 | 2013-11-21 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| AU2014212432B2 (en) | 2013-01-31 | 2019-09-19 | Codexis, Inc. | Methods, systems, and software for identifying bio-molecules using models of multiplicative form |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| US20130189684A1 (en) | 2013-03-12 | 2013-07-25 | Sequenom, Inc. | Quantification of cell-specific nucleic acid markers |
| US9305756B2 (en) | 2013-03-13 | 2016-04-05 | Agena Bioscience, Inc. | Preparation enhancements and methods of use for MALDI mass spectrometry |
| WO2014168711A1 (en) | 2013-03-13 | 2014-10-16 | Sequenom, Inc. | Primers for dna methylation analysis |
| EP2971097B1 (en) | 2013-03-15 | 2018-08-01 | Verinata Health, Inc | Generating cell-free dna libraries directly from blood |
| PL2981921T3 (pl) | 2013-04-03 | 2023-05-08 | Sequenom, Inc. | Metody i procesy nieinwazyjnej oceny zmienności genetycznych |
| US20140336055A1 (en) | 2013-05-07 | 2014-11-13 | Sequenom, Inc. | Genetic markers for macular degeneration disorder treatment |
| AU2014268377B2 (en) | 2013-05-24 | 2020-10-08 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| MY197877A (en) | 2013-06-04 | 2023-07-22 | Harvard College | Rna-guided transcriptional regulation |
| EP3543354B1 (en) | 2013-06-17 | 2022-01-19 | Verinata Health, Inc. | Method for generating a masked reference sequence of the y chromosome |
| SMT201900206T1 (it) | 2013-06-21 | 2019-05-10 | Sequenom Inc | Metodo per la valutazione non invasiva di variazioni genetiche |
| CA2923755C (en) | 2013-09-27 | 2023-03-14 | Codexis, Inc. | Automated screening of enzyme variants |
| PL3053071T3 (pl) | 2013-10-04 | 2024-03-18 | Sequenom, Inc. | Metody i procesy nieinwazyjnej oceny zmienności genetycznych |
| EP3495496B1 (en) | 2013-10-07 | 2020-11-25 | Sequenom, Inc. | Methods and processes for non-invasive assessment of chromosome alterations |
| JP6534191B2 (ja) | 2013-10-21 | 2019-06-26 | ベリナタ ヘルス インコーポレイテッド | コピー数変動を決定することにおける検出の感度を向上させるための方法 |
| EP3077430A4 (en) | 2013-12-05 | 2017-08-16 | Centrillion Technology Holdings Corporation | Modified surfaces |
| CN107002117B (zh) | 2013-12-05 | 2021-12-10 | 生捷科技控股公司 | 核酸测序方法 |
| CN111118121B (zh) | 2013-12-05 | 2024-07-19 | 生捷科技控股公司 | 图案化阵列的制备 |
| CN105917008B (zh) | 2014-01-16 | 2020-11-27 | 启迪公司 | 用于前列腺癌复发的预后的基因表达面板 |
| WO2015138774A1 (en) | 2014-03-13 | 2015-09-17 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US11060139B2 (en) | 2014-03-28 | 2021-07-13 | Centrillion Technology Holdings Corporation | Methods for sequencing nucleic acids |
| CA2950596C (en) | 2014-05-30 | 2023-10-31 | Verinata Health, Inc. | Detecting fetal sub-chromosomal aneuploidies and copy number variations |
| US10179932B2 (en) | 2014-07-11 | 2019-01-15 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| US10119167B2 (en) | 2014-07-18 | 2018-11-06 | Illumina, Inc. | Non-invasive prenatal diagnosis of fetal genetic condition using cellular DNA and cell free DNA |
| EP3175000B1 (en) | 2014-07-30 | 2020-07-29 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US20160032281A1 (en) * | 2014-07-31 | 2016-02-04 | Fei Company | Functionalized grids for locating and imaging biological specimens and methods of using the same |
| ES2688840T3 (es) | 2014-10-09 | 2018-11-07 | F. Hoffmann-La Roche Ag | Mutaciones en el dominio cinasa del receptor del factor de crecimiento epidérmico |
| US11789906B2 (en) | 2014-11-19 | 2023-10-17 | Arc Bio, Llc | Systems and methods for genomic manipulations and analysis |
| EP3502273B1 (en) | 2014-12-12 | 2020-07-08 | Verinata Health, Inc. | Cell-free dna fragment |
| CN104630346A (zh) * | 2015-01-05 | 2015-05-20 | 东南大学 | 基于高分辨透射电子显微学的单分子dna测序方法 |
| CA2994406A1 (en) | 2015-08-06 | 2017-02-09 | Arc Bio, Llc | Systems and methods for genomic analysis |
| US10774375B2 (en) | 2015-09-18 | 2020-09-15 | Agena Bioscience, Inc. | Methods and compositions for the quantitation of mitochondrial nucleic acid |
| EP3371329A4 (en) | 2015-11-03 | 2019-06-19 | President and Fellows of Harvard College | METHOD AND DEVICE FOR VOLUMETRIC IMAGING OF A THREE-DIMENSIONAL NUCLEIC ACID-CONTAINING MATRIX |
| US10095831B2 (en) | 2016-02-03 | 2018-10-09 | Verinata Health, Inc. | Using cell-free DNA fragment size to determine copy number variations |
| US9989482B2 (en) * | 2016-02-16 | 2018-06-05 | General Electric Company | Methods for radiographic and CT inspection of additively manufactured workpieces |
| CN109415761B (zh) | 2016-04-25 | 2022-09-20 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
| EP3491560A1 (en) | 2016-07-27 | 2019-06-05 | Sequenom, Inc. | Genetic copy number alteration classifications |
| CN109983125B (zh) | 2016-08-31 | 2024-06-04 | 哈佛学院董事及会员团体 | 生成用于通过荧光原位测序检测的核酸序列文库的方法 |
| JP7057348B2 (ja) | 2016-08-31 | 2022-04-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 蛍光in situ配列決定を用いた単一アッセイに生体分子の検出を組み合わせる方法 |
| JP6730525B2 (ja) | 2016-11-21 | 2020-07-29 | ナノストリング テクノロジーズ,インコーポレイティド | 化学組成物とそれを利用する方法 |
| EP3574424A1 (en) | 2017-01-24 | 2019-12-04 | Sequenom, Inc. | Methods and processes for assessment of genetic variations |
| EP3998350B1 (en) | 2017-03-17 | 2025-05-07 | Sequenom, Inc. | Methods and processes for assessment of genetic mosaicism |
| EP3448396A4 (en) | 2017-04-14 | 2020-01-22 | President and Fellows of Harvard College | METHODS OF GENERATING A CELL-DERIVED MICROFILAMENT ARRAY |
| US11342047B2 (en) | 2017-04-21 | 2022-05-24 | Illumina, Inc. | Using cell-free DNA fragment size to detect tumor-associated variant |
| SG11201911538YA (en) | 2017-06-20 | 2020-01-30 | Illumina Inc | Methods and systems for decomposition and quantification of dna mixtures from multiple contributors of known or unknown genotypes |
| KR102543270B1 (ko) | 2017-06-20 | 2023-06-13 | 일루미나, 인코포레이티드 | 미지의 유전자형의 기여자로부터의 dna 혼합물의 정확한 컴퓨팅 분해를 위한 방법 |
| KR102805731B1 (ko) | 2017-12-01 | 2025-06-19 | 일루미나, 인코포레이티드 | 체성 돌연변이 클론형성능을 결정하기 위한 방법 및 시스템 |
| SG11202011274YA (en) | 2018-05-14 | 2020-12-30 | Nanostring Technologies Inc | Chemical compositions and methods of using same |
| WO2020028194A1 (en) | 2018-07-30 | 2020-02-06 | Readcoor, Inc. | Methods and systems for sample processing or analysis |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN111324096B (zh) * | 2020-03-03 | 2021-04-23 | 郑州旭飞光电科技有限公司 | 一种基板玻璃加工、包装信息可追溯系统及方法 |
| CN111537529A (zh) * | 2020-04-09 | 2020-08-14 | 中国科学院微电子研究所 | 一种用于附着穿透式电子显微镜样品的硅网及其制备方法 |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| WO2022164968A1 (en) | 2021-01-29 | 2022-08-04 | Day Zero Diagnostics, Inc. | Methods for enriching microorganisms from low abundance clinical samples for dna sequencing |
| US20240392363A1 (en) | 2023-05-26 | 2024-11-28 | Illumina, Inc. | Methods for discriminating between fetal and maternal events in non-invasive prenatal test samples |
Family Cites Families (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4689295A (en) * | 1983-01-20 | 1987-08-25 | Integrated Genetics, Inc. | Test for Salmonella |
| US6221581B1 (en) * | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
| US5447841A (en) * | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US6150089A (en) | 1988-09-15 | 2000-11-21 | New York University | Method and characterizing polymer molecules or the like |
| US5800992A (en) * | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5106729A (en) * | 1989-07-24 | 1992-04-21 | Arizona Board Of Regents Acting On Behalf Of Arizona State University | Method for visualizing the base sequence of nucleic acid polymers |
| US5789161A (en) * | 1990-09-20 | 1998-08-04 | Amoco Corporation | Methods for genome identification using direct label probe composition |
| JPH05244997A (ja) | 1992-03-04 | 1993-09-24 | Hitachi Ltd | Dnaまたはrnaの塩基配列決定法 |
| US5871912A (en) | 1992-04-30 | 1999-02-16 | Institut Pasteur | Nucleic acid probes, sequences and methods for detecting mycobacterium tuberculosis resistant to isoniazid |
| US5472881A (en) * | 1992-11-12 | 1995-12-05 | University Of Utah Research Foundation | Thiol labeling of DNA for attachment to gold surfaces |
| EP0682671A4 (en) * | 1993-02-01 | 1998-01-14 | Seq Ltd | METHOD AND DEVICES FOR SEQUENCING DNA. |
| GB9401200D0 (en) * | 1994-01-21 | 1994-03-16 | Medical Res Council | Sequencing of nucleic acids |
| US20020168642A1 (en) * | 1994-06-06 | 2002-11-14 | Andrzej Drukier | Sequencing duplex DNA by mass spectroscopy |
| WO1996009410A1 (en) | 1994-09-23 | 1996-03-28 | Finkelstein Sydney D | Topographic genotyping |
| US5601982A (en) | 1995-02-07 | 1997-02-11 | Sargent; Jeannine P. | Method and apparatus for determining the sequence of polynucleotides |
| US5843650A (en) | 1995-05-01 | 1998-12-01 | Segev; David | Nucleic acid detection and amplification by chemical linkage of oligonucleotides |
| US6852487B1 (en) * | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| EP1736554B1 (en) | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6582921B2 (en) * | 1996-07-29 | 2003-06-24 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses thereof |
| GB9620209D0 (en) * | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| WO1998033939A1 (en) * | 1997-01-31 | 1998-08-06 | Hitachi, Ltd. | Method for determining nucleic acid base sequence and apparatus therefor |
| CA2205081A1 (en) | 1997-06-04 | 1998-12-04 | Graham Dellaire | A method to map and isolate regions of chromosomes that interact or associate functionally within or between chromosomes in vivo |
| US5903085A (en) * | 1997-06-18 | 1999-05-11 | Phase Metrics, Inc. | Piezoelectric nanopositioner |
| DE19900511C2 (de) | 1999-01-08 | 2001-03-15 | Deutsches Krebsforsch | Molekularbiologische Marker für die analytische Elektronenmikroskopie |
| WO2000075868A2 (en) * | 1999-06-07 | 2000-12-14 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for use in three-dimensional structural determination |
| DE19937512B4 (de) * | 1999-08-09 | 2006-08-24 | Alexander Cherkasky | Verfahren und Gerät zur schnellen Genom-Sequenzierung durch Linearisierung oder Auseinanderziehen der DNA |
| US7244559B2 (en) * | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7211390B2 (en) * | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| AU2001293366A1 (en) * | 2000-04-14 | 2001-10-30 | Cornell Research Foundation, Inc. | Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction |
| JP2002153271A (ja) * | 2000-11-17 | 2002-05-28 | Jeol Ltd | Dnaあるいはrnaの塩基配列決定方法およびdnaシーケンサー |
| US7419833B2 (en) * | 2000-11-17 | 2008-09-02 | Nagayama Ip Holdings Llc | Method for nucleic acid sequencing |
| AU2002227156A1 (en) * | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| CA2430253C (en) * | 2000-12-15 | 2011-04-26 | The Arizona Board Of Regents | Method for patterning metal using nanoparticle containing precursors |
| WO2002075306A1 (en) | 2001-03-16 | 2002-09-26 | Large Scale Biology Corporation | Episomal non-transforming nucleic acid elements in functional genomic and antigenic applications |
| EP2801624B1 (en) * | 2001-03-16 | 2019-03-06 | Singular Bio, Inc | Arrays and methods of use |
| FR2823223B1 (fr) * | 2001-04-04 | 2004-03-12 | Centre Nat Rech Scient | Structure bicouche supportee de presentation d'un acide nucleique associe a une proteine |
| GB0112238D0 (en) * | 2001-05-18 | 2001-07-11 | Medical Biosystems Ltd | Sequencing method |
| GB2378245A (en) * | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| FR2829580B1 (fr) | 2001-09-07 | 2004-02-13 | Bio Merieux | Procede de lecture, de detection ou de quantification, hybrides ou complexes utilises dans ce procede et biopuce mettant en oeuvre ledit procede |
| AU2002332846A1 (en) * | 2001-09-07 | 2003-04-07 | Boston University | Lanthanide binding tags |
| AU2003215388A1 (en) * | 2002-02-22 | 2003-09-09 | Purdue Research Foundation | Magnetic nanomaterials and methods for detection of biological materials |
| JP4120240B2 (ja) | 2002-03-06 | 2008-07-16 | 株式会社島津製作所 | 生体由来異物検査法 |
| US20060003437A1 (en) * | 2002-06-26 | 2006-01-05 | Fujitsu Limited | Target detecting device and target capturer, device and method for molecular adsorption or desorption, and device and method for protein detection |
| US20040081985A1 (en) | 2002-07-12 | 2004-04-29 | Affymetrix, Inc. | Systems and method for examination of microarrays using scanning electron microscope |
| US20050009055A1 (en) | 2002-07-12 | 2005-01-13 | Affymetrix, Inc. | System and method for examination of microarrays using scanning electron microscope |
| WO2004027093A1 (en) | 2002-09-19 | 2004-04-01 | The Chancellor, Master And Scholars Of The University Of Oxford | Molecular arrays and single molecule detection |
| EP1424671A1 (en) * | 2002-11-29 | 2004-06-02 | Alrec Sign & Display B.V. | Illuminated sign |
| JP2004212355A (ja) * | 2003-01-09 | 2004-07-29 | Hitachi Ltd | バイオ電子顕微鏡及び試料の観察方法 |
| US20100035254A1 (en) * | 2003-04-08 | 2010-02-11 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| US7745116B2 (en) * | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| EP1618210B1 (en) * | 2003-04-21 | 2010-09-01 | Roche Diagnostics GmbH | Associations of polymorphisms in the frzb gene with osteoporosis |
| US7625346B2 (en) * | 2003-05-30 | 2009-12-01 | Boston Scientific Scimed, Inc. | Transbronchial needle aspiration device |
| WO2005052582A2 (en) | 2003-11-28 | 2005-06-09 | Pamgene B.V. | Methods and apparatus for cell based microarray assays |
| US20050147977A1 (en) * | 2003-12-29 | 2005-07-07 | Tae-Woong Koo | Methods and compositions for nucleic acid detection and sequence analysis |
| US7381529B2 (en) * | 2003-12-31 | 2008-06-03 | Intel Corporation | Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes |
| US7154091B2 (en) * | 2004-04-02 | 2006-12-26 | California Institute Of Technology | Method and system for ultrafast photoelectron microscope |
| WO2005118877A2 (en) * | 2004-06-02 | 2005-12-15 | Vicus Bioscience, Llc | Producing, cataloging and classifying sequence tags |
| US8697432B2 (en) | 2004-07-14 | 2014-04-15 | Zs Genetics, Inc. | Systems and methods of analyzing nucleic acid polymers and related components |
| JP4563117B2 (ja) * | 2004-09-03 | 2010-10-13 | エスアイアイ・ナノテクノロジー株式会社 | 顕微鏡システム及び顕微鏡システムの走査法ならびに顕微鏡システムの画像合成法 |
| WO2006118615A2 (en) * | 2004-12-16 | 2006-11-09 | Brandeis University | Clonable tag for purification and electron microscopy labeling |
| US7875426B2 (en) | 2005-02-04 | 2011-01-25 | University Of South Florida | DNA biochip and methods of use |
| JP2006262834A (ja) * | 2005-03-25 | 2006-10-05 | Canon Inc | 核酸の塩基配列の決定方法及び該方法に基づく核酸の塩基配列解析装置 |
| US20060287833A1 (en) | 2005-06-17 | 2006-12-21 | Zohar Yakhini | Method and system for sequencing nucleic acid molecules using sequencing by hybridization and comparison with decoration patterns |
| EP1922407A2 (en) | 2005-09-09 | 2008-05-21 | Keygene N.V. | Homologous recombination in plants |
| JP2009513141A (ja) | 2005-10-27 | 2009-04-02 | トラスティーズ オブ ボストン ユニバーシティ | タンパク質相補によるリアルタイムインビボ核酸検出 |
| WO2007120202A2 (en) * | 2005-11-09 | 2007-10-25 | Zs Genetics, Inc. | Nano-scale ligand arrays on substrates for particle beam instruments and related methods |
| CN106443006A (zh) * | 2005-11-16 | 2017-02-22 | Ambrx公司 | 包括非天然氨基酸的方法和组合物 |
| US20070128610A1 (en) * | 2005-12-02 | 2007-06-07 | Buzby Philip R | Sample preparation method and apparatus for nucleic acid sequencing |
| WO2007089542A2 (en) * | 2006-01-27 | 2007-08-09 | Zs Genetics, Inc. | Systems and methods of analyzing nucleic acid polymers by using particle beams |
| ES2301342B1 (es) | 2006-03-14 | 2009-05-01 | Oryzon Genomics, S.A. | "metodo de analisis de acidos nucleicos". |
| EP1840223B1 (de) | 2006-03-25 | 2009-11-18 | Ruprecht-Karls-Universität Heidelberg | Verfahren zur mikroskopischen Ortsbestimmung eines ausgewählten, intrazellulären DNA-Abschnitts bekannter Nukleotidsequenz |
| JP5092462B2 (ja) * | 2006-06-13 | 2012-12-05 | 株式会社デンソー | 力学量センサ |
| WO2008011430A2 (en) | 2006-07-17 | 2008-01-24 | Fox Chase Cancer Center | Compositions and methods for the treatment of diseases associated with aberrant cilia assembly and regulation |
| JP2010510476A (ja) * | 2006-07-19 | 2010-04-02 | バイオナノマトリックス、インク. | ナノノズル装置アレイ:その作製および高分子分析における利用 |
| WO2008022035A2 (en) | 2006-08-10 | 2008-02-21 | The Scripps Research Institute | Methods for identifying cellular modulators of disaggregation activity or aggregation activity in an animal |
| US20080060778A1 (en) * | 2006-09-08 | 2008-03-13 | Abraham Velasco-Tellez | Binder composition and method of forming foundry sand cores and molds |
| US20090208965A1 (en) * | 2006-10-25 | 2009-08-20 | Ikonisys, Inc. | Automated method for detecting cancers and high grade hyperplasias |
| US20090215643A1 (en) * | 2007-07-26 | 2009-08-27 | Cellay, Llc | Highly Visible Chromosome-Specific Probes and Related Methods |
| US8951731B2 (en) | 2007-10-15 | 2015-02-10 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| JP2009178159A (ja) | 2007-11-05 | 2009-08-13 | Hitachi Plant Technologies Ltd | 核酸配列の検出方法及び核酸配列検出基板 |
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2008
- 2008-10-06 EA EA201000427A patent/EA201000427A1/ru unknown
- 2008-10-06 WO PCT/US2008/078986 patent/WO2009046445A1/en not_active Ceased
- 2008-10-06 CA CA2701726A patent/CA2701726A1/en not_active Abandoned
- 2008-10-06 CN CN2008801193343A patent/CN101889074A/zh active Pending
- 2008-10-06 EP EP08835273A patent/EP2203547A4/en not_active Withdrawn
- 2008-10-06 MX MX2010003724A patent/MX2010003724A/es unknown
- 2008-10-06 US US12/681,589 patent/US20100331195A1/en not_active Abandoned
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- 2008-10-06 AU AU2008308457A patent/AU2008308457A1/en not_active Abandoned
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013069773A1 (ja) * | 2011-11-11 | 2013-05-16 | ナガヤマ アイピー ホールディングス エルエルシー | 塩基対応標識方法、塩基配列情報取得方法及び塩基対応標識化一本鎖核酸 |
| JP2015514653A (ja) * | 2012-01-19 | 2015-05-21 | ジェームズ ダーリング、マイケル | グラフェンナノ構造のdna画定エッチング法 |
| JP2018141782A (ja) * | 2017-02-27 | 2018-09-13 | 住友金属鉱山株式会社 | 汚染堆積物の定量方法および試料分析方法 |
| JP7024490B2 (ja) | 2017-02-27 | 2022-02-24 | 住友金属鉱山株式会社 | 汚染堆積物の定量方法および試料分析方法 |
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| US20100267154A1 (en) | 2010-10-21 |
| EP2203547A1 (en) | 2010-07-07 |
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| US20100267155A1 (en) | 2010-10-21 |
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| WO2009046445A1 (en) | 2009-04-09 |
| EP2203547A4 (en) | 2011-06-01 |
| US20100267156A1 (en) | 2010-10-21 |
| US20100331195A1 (en) | 2010-12-30 |
| AU2008308457A1 (en) | 2009-04-09 |
| CN101889074A (zh) | 2010-11-17 |
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