KR20100089060A - 전자 현미경으로 핵산 중합체를 시퀀싱하는 방법 - Google Patents
전자 현미경으로 핵산 중합체를 시퀀싱하는 방법 Download PDFInfo
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- KR20100089060A KR20100089060A KR1020107008686A KR20107008686A KR20100089060A KR 20100089060 A KR20100089060 A KR 20100089060A KR 1020107008686 A KR1020107008686 A KR 1020107008686A KR 20107008686 A KR20107008686 A KR 20107008686A KR 20100089060 A KR20100089060 A KR 20100089060A
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- nucleic acid
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Families Citing this family (109)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8021841B1 (en) * | 2007-07-11 | 2011-09-20 | Kenneth David Schatz | Methods and apparatuses for spatially separating molecules attached to a support and uses in biotechnology |
| US7888127B2 (en) | 2008-01-15 | 2011-02-15 | Sequenom, Inc. | Methods for reducing adduct formation for mass spectrometry analysis |
| EP3216874A1 (en) | 2008-09-05 | 2017-09-13 | TOMA Biosciences, Inc. | Methods for stratifying and annotating cancer drug treatment options |
| US8962247B2 (en) | 2008-09-16 | 2015-02-24 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses |
| US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| US9926593B2 (en) | 2009-12-22 | 2018-03-27 | Sequenom, Inc. | Processes and kits for identifying aneuploidy |
| US9260745B2 (en) | 2010-01-19 | 2016-02-16 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| AU2011207561B2 (en) | 2010-01-19 | 2014-02-20 | Verinata Health, Inc. | Partition defined detection methods |
| US10388403B2 (en) | 2010-01-19 | 2019-08-20 | Verinata Health, Inc. | Analyzing copy number variation in the detection of cancer |
| US20120100548A1 (en) | 2010-10-26 | 2012-04-26 | Verinata Health, Inc. | Method for determining copy number variations |
| WO2011090556A1 (en) | 2010-01-19 | 2011-07-28 | Verinata Health, Inc. | Methods for determining fraction of fetal nucleic acid in maternal samples |
| US9323888B2 (en) | 2010-01-19 | 2016-04-26 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| EP2513341B1 (en) | 2010-01-19 | 2017-04-12 | Verinata Health, Inc | Identification of polymorphic sequences in mixtures of genomic dna by whole genome sequencing |
| AU2010343276B2 (en) | 2010-01-19 | 2015-05-28 | Verinata Health, Inc. | Methods for determining fraction of fetal nucleic acid in maternal samples |
| CN103843105A (zh) | 2010-02-10 | 2014-06-04 | 摩奇有限公司(d/b/aVoxa) | 暗视野像差矫正电子显微镜 |
| EP3572528A1 (en) | 2010-09-24 | 2019-11-27 | The Board of Trustees of the Leland Stanford Junior University | Direct capture, amplification and sequencing of target dna using immobilized primers |
| WO2012064850A1 (en) * | 2010-11-09 | 2012-05-18 | Michael Andregg | Liquid-gas interface mediated molecule manipulation |
| US20140121132A1 (en) * | 2011-03-11 | 2014-05-01 | Cornell University | Systems and methods for high resolution biomolecular imaging and analysis |
| US20150065358A1 (en) | 2011-03-30 | 2015-03-05 | Verinata Health, Inc. | Method for verifying bioassay samples |
| PT3456844T (pt) | 2011-04-12 | 2020-08-25 | Verinata Health Inc | Resolução de frações de genoma utilizando contagens de polimorfismos |
| CN102985561B (zh) | 2011-04-14 | 2015-04-01 | 维里纳塔健康公司 | 用于确定并且验证常见的和罕见的染色体非整倍性的归一化染色体 |
| US9411937B2 (en) | 2011-04-15 | 2016-08-09 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| US20140235474A1 (en) | 2011-06-24 | 2014-08-21 | Sequenom, Inc. | Methods and processes for non invasive assessment of a genetic variation |
| US20130029348A1 (en) * | 2011-07-26 | 2013-01-31 | Opgen, Inc. | Methods of elongating nucleic acids |
| KR101974492B1 (ko) | 2011-07-26 | 2019-05-02 | 베리나타 헬스, 인코포레이티드 | 샘플 중 상이한 이수성의 존재 또는 부재를 결정하는 방법 |
| US9367663B2 (en) | 2011-10-06 | 2016-06-14 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10424394B2 (en) | 2011-10-06 | 2019-09-24 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| WO2013052913A2 (en) | 2011-10-06 | 2013-04-11 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10196681B2 (en) | 2011-10-06 | 2019-02-05 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9984198B2 (en) | 2011-10-06 | 2018-05-29 | Sequenom, Inc. | Reducing sequence read count error in assessment of complex genetic variations |
| US20140242588A1 (en) | 2011-10-06 | 2014-08-28 | Sequenom, Inc | Methods and processes for non-invasive assessment of genetic variations |
| DK2766496T3 (en) | 2011-10-11 | 2017-05-15 | Sequenom Inc | METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS |
| US8688388B2 (en) | 2011-10-11 | 2014-04-01 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| JP2014531908A (ja) | 2011-10-14 | 2014-12-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 構造アッセンブリによる配列決定 |
| CN103945930B (zh) * | 2011-10-25 | 2016-10-05 | 亚利桑那董事会,代表亚利桑那州立大学行事的亚利桑那州法人团体 | 可编程的阵列 |
| JP2014176297A (ja) * | 2011-11-11 | 2014-09-25 | Nagayama Ip Holdings Llc | 塩基対応標識方法、塩基配列情報取得方法及び塩基対応標識化一本鎖核酸 |
| JP6034801B2 (ja) * | 2011-11-15 | 2016-11-30 | ナガヤマ アイピー ホールディングス エルエルシーNagayama IP Holdings, LLC | 塩基配列決定装置 |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP4249605B1 (en) | 2011-12-22 | 2024-08-28 | President And Fellows Of Harvard College | Methods for analyte detection |
| US8858778B2 (en) * | 2012-01-19 | 2014-10-14 | Michael James Darling | Method for DNA defined etching of a graphene nanostructure |
| EP2805280B1 (en) | 2012-01-20 | 2022-10-05 | Sequenom, Inc. | Diagnostic processes that factor experimental conditions |
| WO2013112999A1 (en) * | 2012-01-27 | 2013-08-01 | Cornell University | Methods and arrays for controlled manipulation of dna and chromatin fragments for genetic and epigenetic analysis |
| HK1206055A1 (en) | 2012-03-02 | 2015-12-31 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| US10497461B2 (en) | 2012-06-22 | 2019-12-03 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| WO2014011928A1 (en) | 2012-07-13 | 2014-01-16 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| EP2875149B1 (en) | 2012-07-20 | 2019-12-04 | Verinata Health, Inc. | Detecting and classifying copy number variation in a cancer genome |
| US10482994B2 (en) | 2012-10-04 | 2019-11-19 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| EP2904534B1 (en) | 2012-10-04 | 2021-12-15 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US20130309666A1 (en) | 2013-01-25 | 2013-11-21 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| AU2014212430B2 (en) | 2013-01-31 | 2019-06-20 | Codexis, Inc. | Methods, systems, and software for identifying bio-molecules with interacting components |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| US20130189684A1 (en) | 2013-03-12 | 2013-07-25 | Sequenom, Inc. | Quantification of cell-specific nucleic acid markers |
| US9305756B2 (en) | 2013-03-13 | 2016-04-05 | Agena Bioscience, Inc. | Preparation enhancements and methods of use for MALDI mass spectrometry |
| EP2971100A1 (en) | 2013-03-13 | 2016-01-20 | Sequenom, Inc. | Primers for dna methylation analysis |
| CA2906818C (en) | 2013-03-15 | 2022-08-16 | Verinata Health, Inc. | Generating cell-free dna libraries directly from blood |
| ES2939547T3 (es) | 2013-04-03 | 2023-04-24 | Sequenom Inc | Métodos y procedimientos para la evaluación no invasiva de variaciones genéticas |
| US20140336055A1 (en) | 2013-05-07 | 2014-11-13 | Sequenom, Inc. | Genetic markers for macular degeneration disorder treatment |
| IL300163B2 (en) | 2013-05-24 | 2024-06-01 | Sequenom Inc | Methods and processes for non-invasive assessment of genetic variations |
| EP3003392B1 (en) | 2013-06-04 | 2019-10-23 | President and Fellows of Harvard College | Rna-guideded transcriptional regulation |
| CA2915626A1 (en) | 2013-06-17 | 2014-12-24 | Verinata Health, Inc. | Method for determining copy number variations in sex chromosomes |
| MX2015016911A (es) | 2013-06-21 | 2016-06-21 | Sequenom Inc | Metodos y procesos para evaluacion no invasiva de variaciones geneticas. |
| ES2693150T3 (es) | 2013-09-27 | 2018-12-07 | Codexis, Inc. | Filtración automática de variantes de enzimas |
| IL289974B (en) | 2013-10-04 | 2022-09-01 | Sequenom Inc | Methods and processes for non-invasive evaluation of genetic variations |
| EP3055427B1 (en) | 2013-10-07 | 2018-09-12 | Sequenom, Inc. | Methods and processes for non-invasive assessment of chromosome alterations |
| US10741269B2 (en) | 2013-10-21 | 2020-08-11 | Verinata Health, Inc. | Method for improving the sensitivity of detection in determining copy number variations |
| US10597715B2 (en) | 2013-12-05 | 2020-03-24 | Centrillion Technology Holdings | Methods for sequencing nucleic acids |
| EP3628747B1 (en) | 2013-12-05 | 2022-10-05 | Centrillion Technology Holdings Corporation | Fabrication of patterned arrays |
| CN105940024B (zh) | 2013-12-05 | 2019-03-15 | 生捷科技控股公司 | 修饰的表面 |
| CN105917008B (zh) | 2014-01-16 | 2020-11-27 | 启迪公司 | 用于前列腺癌复发的预后的基因表达面板 |
| EP3736344A1 (en) | 2014-03-13 | 2020-11-11 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US11060139B2 (en) | 2014-03-28 | 2021-07-13 | Centrillion Technology Holdings Corporation | Methods for sequencing nucleic acids |
| US10318704B2 (en) | 2014-05-30 | 2019-06-11 | Verinata Health, Inc. | Detecting fetal sub-chromosomal aneuploidies |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| EP3940703A1 (en) | 2014-07-18 | 2022-01-19 | Illumina, Inc. | Non-invasive prenatal diagnosis of fetal genetic conditions using cellular dna and cell free dna |
| EP3760739B1 (en) | 2014-07-30 | 2025-09-03 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US20160032281A1 (en) * | 2014-07-31 | 2016-02-04 | Fei Company | Functionalized grids for locating and imaging biological specimens and methods of using the same |
| ES2688840T3 (es) | 2014-10-09 | 2018-11-07 | F. Hoffmann-La Roche Ag | Mutaciones en el dominio cinasa del receptor del factor de crecimiento epidérmico |
| US11789906B2 (en) | 2014-11-19 | 2023-10-17 | Arc Bio, Llc | Systems and methods for genomic manipulations and analysis |
| WO2016094853A1 (en) | 2014-12-12 | 2016-06-16 | Verinata Health, Inc. | Using cell-free dna fragment size to determine copy number variations |
| CN104630346A (zh) * | 2015-01-05 | 2015-05-20 | 东南大学 | 基于高分辨透射电子显微学的单分子dna测序方法 |
| EP3332034B1 (en) | 2015-08-06 | 2024-09-25 | Arc Bio, LLC | Systems and methods for genomic analysis |
| WO2017049180A1 (en) | 2015-09-18 | 2017-03-23 | Agena Bioscience, Inc. | Methods and compositions for the quantitation of mitochondrial nucleic acid |
| JP6882282B2 (ja) | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 三次元核酸含有マトリックスの立体撮像のための方法と装置 |
| US10095831B2 (en) | 2016-02-03 | 2018-10-09 | Verinata Health, Inc. | Using cell-free DNA fragment size to determine copy number variations |
| US9989482B2 (en) * | 2016-02-16 | 2018-06-05 | General Electric Company | Methods for radiographic and CT inspection of additively manufactured workpieces |
| EP3449016B1 (en) | 2016-04-25 | 2025-07-23 | President and Fellows of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| CA3030890A1 (en) | 2016-07-27 | 2018-02-01 | Sequenom, Inc. | Genetic copy number alteration classifications |
| JP7239465B2 (ja) | 2016-08-31 | 2023-03-14 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 蛍光in situ配列決定による検出のための核酸配列ライブラリの作製法 |
| WO2018045186A1 (en) | 2016-08-31 | 2018-03-08 | President And Fellows Of Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| US10415080B2 (en) | 2016-11-21 | 2019-09-17 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
| JP7237003B2 (ja) | 2017-01-24 | 2023-03-10 | セクエノム, インコーポレイテッド | 遺伝子片の評価のための方法およびプロセス |
| JP7024490B2 (ja) * | 2017-02-27 | 2022-02-24 | 住友金属鉱山株式会社 | 汚染堆積物の定量方法および試料分析方法 |
| US12421550B2 (en) | 2017-03-17 | 2025-09-23 | Sequenom, Inc. | Methods and processes for assessment of genetic mosaicism |
| KR20190140906A (ko) | 2017-04-14 | 2019-12-20 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 세포-유래 마이크로필라멘트 네트워크의 생성 방법 |
| US11342047B2 (en) | 2017-04-21 | 2022-05-24 | Illumina, Inc. | Using cell-free DNA fragment size to detect tumor-associated variant |
| SG11201911538YA (en) | 2017-06-20 | 2020-01-30 | Illumina Inc | Methods and systems for decomposition and quantification of dna mixtures from multiple contributors of known or unknown genotypes |
| KR102543270B1 (ko) | 2017-06-20 | 2023-06-13 | 일루미나, 인코포레이티드 | 미지의 유전자형의 기여자로부터의 dna 혼합물의 정확한 컴퓨팅 분해를 위한 방법 |
| US12367978B2 (en) | 2017-12-01 | 2025-07-22 | Illumina, Inc. | Methods and systems for determining somatic mutation clonality |
| KR102893736B1 (ko) | 2018-05-14 | 2025-11-28 | 브루커 스페이셜 바이올로지, 인크. | 화학 조성물 및 이의 사용 방법 |
| EP3836967A4 (en) | 2018-07-30 | 2022-06-15 | ReadCoor, LLC | METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN111324096B (zh) * | 2020-03-03 | 2021-04-23 | 郑州旭飞光电科技有限公司 | 一种基板玻璃加工、包装信息可追溯系统及方法 |
| CN111537529A (zh) * | 2020-04-09 | 2020-08-14 | 中国科学院微电子研究所 | 一种用于附着穿透式电子显微镜样品的硅网及其制备方法 |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| WO2022164968A1 (en) | 2021-01-29 | 2022-08-04 | Day Zero Diagnostics, Inc. | Methods for enriching microorganisms from low abundance clinical samples for dna sequencing |
| WO2024249175A1 (en) | 2023-05-26 | 2024-12-05 | Illumina, Inc. | Methods for discriminating between fetal and maternal events in non-invasive prenatal test samples |
Family Cites Families (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4689295A (en) * | 1983-01-20 | 1987-08-25 | Integrated Genetics, Inc. | Test for Salmonella |
| US6221581B1 (en) * | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
| US5447841A (en) * | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US6150089A (en) * | 1988-09-15 | 2000-11-21 | New York University | Method and characterizing polymer molecules or the like |
| US5800992A (en) * | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5106729A (en) * | 1989-07-24 | 1992-04-21 | Arizona Board Of Regents Acting On Behalf Of Arizona State University | Method for visualizing the base sequence of nucleic acid polymers |
| US5789161A (en) * | 1990-09-20 | 1998-08-04 | Amoco Corporation | Methods for genome identification using direct label probe composition |
| JPH05244997A (ja) * | 1992-03-04 | 1993-09-24 | Hitachi Ltd | Dnaまたはrnaの塩基配列決定法 |
| US5871912A (en) * | 1992-04-30 | 1999-02-16 | Institut Pasteur | Nucleic acid probes, sequences and methods for detecting mycobacterium tuberculosis resistant to isoniazid |
| US5472881A (en) * | 1992-11-12 | 1995-12-05 | University Of Utah Research Foundation | Thiol labeling of DNA for attachment to gold surfaces |
| JPH08506664A (ja) * | 1993-02-01 | 1996-07-16 | セック,リミテッド | Dna配列決定の方法および装置 |
| GB9401200D0 (en) * | 1994-01-21 | 1994-03-16 | Medical Res Council | Sequencing of nucleic acids |
| US20020168642A1 (en) | 1994-06-06 | 2002-11-14 | Andrzej Drukier | Sequencing duplex DNA by mass spectroscopy |
| WO1996009410A1 (en) * | 1994-09-23 | 1996-03-28 | Finkelstein Sydney D | Topographic genotyping |
| US5601982A (en) * | 1995-02-07 | 1997-02-11 | Sargent; Jeannine P. | Method and apparatus for determining the sequence of polynucleotides |
| US5843650A (en) * | 1995-05-01 | 1998-12-01 | Segev; David | Nucleic acid detection and amplification by chemical linkage of oligonucleotides |
| US6852487B1 (en) * | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| AU730633B2 (en) * | 1996-05-29 | 2001-03-08 | Phillip Belgrader | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6582921B2 (en) | 1996-07-29 | 2003-06-24 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses thereof |
| GB9620209D0 (en) * | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| US6136543A (en) * | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
| CA2205081A1 (en) * | 1997-06-04 | 1998-12-04 | Graham Dellaire | A method to map and isolate regions of chromosomes that interact or associate functionally within or between chromosomes in vivo |
| US5903085A (en) * | 1997-06-18 | 1999-05-11 | Phase Metrics, Inc. | Piezoelectric nanopositioner |
| DE19900511C2 (de) * | 1999-01-08 | 2001-03-15 | Deutsches Krebsforsch | Molekularbiologische Marker für die analytische Elektronenmikroskopie |
| WO2000075868A2 (en) * | 1999-06-07 | 2000-12-14 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for use in three-dimensional structural determination |
| DE19937512B4 (de) * | 1999-08-09 | 2006-08-24 | Alexander Cherkasky | Verfahren und Gerät zur schnellen Genom-Sequenzierung durch Linearisierung oder Auseinanderziehen der DNA |
| US7244559B2 (en) * | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7211390B2 (en) * | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| JP5508654B2 (ja) * | 2000-04-14 | 2014-06-04 | コーネル・リサーチ・ファンデーション・インコーポレイテッド | リガーゼ検出反応を用いた核酸配列の違いの検出のための位置特定可能なアレイの設計方法 |
| JP2002153271A (ja) * | 2000-11-17 | 2002-05-28 | Jeol Ltd | Dnaあるいはrnaの塩基配列決定方法およびdnaシーケンサー |
| US7419833B2 (en) * | 2000-11-17 | 2008-09-02 | Nagayama Ip Holdings Llc | Method for nucleic acid sequencing |
| EP1354064A2 (en) * | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| KR100803186B1 (ko) * | 2000-12-15 | 2008-02-14 | 디 아리조나 보드 오브 리전츠 | 나노입자 함유 전구체를 사용한 금속의 패턴형성 방법 |
| WO2002075306A1 (en) * | 2001-03-16 | 2002-09-26 | Large Scale Biology Corporation | Episomal non-transforming nucleic acid elements in functional genomic and antigenic applications |
| EP2801624B1 (en) * | 2001-03-16 | 2019-03-06 | Singular Bio, Inc | Arrays and methods of use |
| FR2823223B1 (fr) * | 2001-04-04 | 2004-03-12 | Centre Nat Rech Scient | Structure bicouche supportee de presentation d'un acide nucleique associe a une proteine |
| GB0112238D0 (en) * | 2001-05-18 | 2001-07-11 | Medical Biosystems Ltd | Sequencing method |
| GB2378245A (en) * | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| US7101667B2 (en) * | 2001-09-07 | 2006-09-05 | Massachusetts Institute Of Technology | Lanthanide binding tags |
| FR2829580B1 (fr) * | 2001-09-07 | 2004-02-13 | Bio Merieux | Procede de lecture, de detection ou de quantification, hybrides ou complexes utilises dans ce procede et biopuce mettant en oeuvre ledit procede |
| US20040086885A1 (en) * | 2002-02-22 | 2004-05-06 | Purdue Research Foundation | Magnetic nanomaterials and methods for detection of biological materials |
| JP4120240B2 (ja) * | 2002-03-06 | 2008-07-16 | 株式会社島津製作所 | 生体由来異物検査法 |
| US20060003437A1 (en) * | 2002-06-26 | 2006-01-05 | Fujitsu Limited | Target detecting device and target capturer, device and method for molecular adsorption or desorption, and device and method for protein detection |
| US20050009055A1 (en) * | 2002-07-12 | 2005-01-13 | Affymetrix, Inc. | System and method for examination of microarrays using scanning electron microscope |
| US20040081985A1 (en) * | 2002-07-12 | 2004-04-29 | Affymetrix, Inc. | Systems and method for examination of microarrays using scanning electron microscope |
| EP1556506A1 (en) * | 2002-09-19 | 2005-07-27 | The Chancellor, Masters And Scholars Of The University Of Oxford | Molecular arrays and single molecule detection |
| EP1424671A1 (en) * | 2002-11-29 | 2004-06-02 | Alrec Sign & Display B.V. | Illuminated sign |
| JP2004212355A (ja) * | 2003-01-09 | 2004-07-29 | Hitachi Ltd | バイオ電子顕微鏡及び試料の観察方法 |
| US20100035254A1 (en) * | 2003-04-08 | 2010-02-11 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| US7745116B2 (en) * | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| CA2521329C (en) * | 2003-04-21 | 2015-11-24 | F. Hoffmann-La Roche Ag | Associations of polymorphisms in the frzb gene with osteoporosis |
| US7625346B2 (en) * | 2003-05-30 | 2009-12-01 | Boston Scientific Scimed, Inc. | Transbronchial needle aspiration device |
| WO2005052582A2 (en) * | 2003-11-28 | 2005-06-09 | Pamgene B.V. | Methods and apparatus for cell based microarray assays |
| US20050147977A1 (en) * | 2003-12-29 | 2005-07-07 | Tae-Woong Koo | Methods and compositions for nucleic acid detection and sequence analysis |
| US7381529B2 (en) * | 2003-12-31 | 2008-06-03 | Intel Corporation | Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes |
| EP1735811B1 (en) | 2004-04-02 | 2015-09-09 | California Institute Of Technology | Method and system for ultrafast photoelectron microscope |
| US7618778B2 (en) | 2004-06-02 | 2009-11-17 | Kaufman Joseph C | Producing, cataloging and classifying sequence tags |
| JP5224443B2 (ja) * | 2004-07-14 | 2013-07-03 | ゼットエス ジェネティクス,インコーポレーテッド | 核酸ポリマーの分析システムおよび方法および関連構成要素 |
| JP4563117B2 (ja) * | 2004-09-03 | 2010-10-13 | エスアイアイ・ナノテクノロジー株式会社 | 顕微鏡システム及び顕微鏡システムの走査法ならびに顕微鏡システムの画像合成法 |
| US20090239215A1 (en) * | 2004-12-16 | 2009-09-24 | Brandeis University | Clonable Tag for Purification and Electron Microscopy Labeling |
| US7875426B2 (en) * | 2005-02-04 | 2011-01-25 | University Of South Florida | DNA biochip and methods of use |
| JP2006262834A (ja) * | 2005-03-25 | 2006-10-05 | Canon Inc | 核酸の塩基配列の決定方法及び該方法に基づく核酸の塩基配列解析装置 |
| US20060287833A1 (en) * | 2005-06-17 | 2006-12-21 | Zohar Yakhini | Method and system for sequencing nucleic acid molecules using sequencing by hybridization and comparison with decoration patterns |
| WO2007030014A2 (en) * | 2005-09-09 | 2007-03-15 | Keygene N.V. | Homologous recombination in plants |
| AU2006305901A1 (en) * | 2005-10-27 | 2007-05-03 | Trustees Of Boston University | Real time nucleic acid detection in vivo using protein complementation |
| WO2007120202A2 (en) * | 2005-11-09 | 2007-10-25 | Zs Genetics, Inc. | Nano-scale ligand arrays on substrates for particle beam instruments and related methods |
| CN101454461A (zh) | 2005-11-16 | 2009-06-10 | Ambrx公司 | 包括非天然氨基酸的方法和组合物 |
| US20070128610A1 (en) * | 2005-12-02 | 2007-06-07 | Buzby Philip R | Sample preparation method and apparatus for nucleic acid sequencing |
| WO2007089542A2 (en) * | 2006-01-27 | 2007-08-09 | Zs Genetics, Inc. | Systems and methods of analyzing nucleic acid polymers by using particle beams |
| ES2301342B1 (es) * | 2006-03-14 | 2009-05-01 | Oryzon Genomics, S.A. | "metodo de analisis de acidos nucleicos". |
| DE502006005397D1 (de) * | 2006-03-25 | 2009-12-31 | Univ Ruprecht Karls Heidelberg | Verfahren zur mikroskopischen Ortsbestimmung eines ausgewählten, intrazellulären DNA-Abschnitts bekannter Nukleotidsequenz |
| JP5092462B2 (ja) * | 2006-06-13 | 2012-12-05 | 株式会社デンソー | 力学量センサ |
| US8685658B2 (en) * | 2006-07-17 | 2014-04-01 | Fox Chase Cancer Center | Compositions and methods for the treatment of diseases associated with aberrant cilia assembly and regulation |
| CN103203256B (zh) * | 2006-07-19 | 2015-09-23 | 博纳基因技术有限公司 | 纳米口装置阵列:它们的制备以及在大分子分析中的应用 |
| WO2008022035A2 (en) * | 2006-08-10 | 2008-02-21 | The Scripps Research Institute | Methods for identifying cellular modulators of disaggregation activity or aggregation activity in an animal |
| US20080060778A1 (en) * | 2006-09-08 | 2008-03-13 | Abraham Velasco-Tellez | Binder composition and method of forming foundry sand cores and molds |
| US20090208965A1 (en) * | 2006-10-25 | 2009-08-20 | Ikonisys, Inc. | Automated method for detecting cancers and high grade hyperplasias |
| WO2009015359A2 (en) * | 2007-07-26 | 2009-01-29 | Cellay, Llc | Highly visible chromosome-specific probes and related methods |
| WO2009052214A2 (en) * | 2007-10-15 | 2009-04-23 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| JP2009178159A (ja) * | 2007-11-05 | 2009-08-13 | Hitachi Plant Technologies Ltd | 核酸配列の検出方法及び核酸配列検出基板 |
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| US20100267156A1 (en) | 2010-10-21 |
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| US20100267157A1 (en) | 2010-10-21 |
| CN101889074A (zh) | 2010-11-17 |
| WO2009046445A1 (en) | 2009-04-09 |
| ZA201002881B (en) | 2011-07-27 |
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