JP2010539956A5 - - Google Patents
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- Publication number
- JP2010539956A5 JP2010539956A5 JP2010527295A JP2010527295A JP2010539956A5 JP 2010539956 A5 JP2010539956 A5 JP 2010539956A5 JP 2010527295 A JP2010527295 A JP 2010527295A JP 2010527295 A JP2010527295 A JP 2010527295A JP 2010539956 A5 JP2010539956 A5 JP 2010539956A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- oligonucleotide
- facilitator
- sequence
- initiator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims 57
- 239000013615 primer Substances 0.000 claims 57
- 238000000034 method Methods 0.000 claims 31
- 239000003999 initiator Substances 0.000 claims 22
- 230000015572 biosynthetic process Effects 0.000 claims 20
- 238000003786 synthesis reaction Methods 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 17
- 108020004707 nucleic acids Proteins 0.000 claims 16
- 150000007523 nucleic acids Chemical class 0.000 claims 16
- 102000039446 nucleic acids Human genes 0.000 claims 16
- 230000004048 modification Effects 0.000 claims 15
- 238000012986 modification Methods 0.000 claims 15
- 239000002773 nucleotide Substances 0.000 claims 15
- 230000001419 dependent effect Effects 0.000 claims 14
- 102000005877 Peptide Initiation Factors Human genes 0.000 claims 10
- 108010044843 Peptide Initiation Factors Proteins 0.000 claims 10
- 230000000295 complement effect Effects 0.000 claims 10
- 230000003321 amplification Effects 0.000 claims 9
- 238000003199 nucleic acid amplification method Methods 0.000 claims 9
- 239000003155 DNA primer Substances 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 4
- 230000002255 enzymatic effect Effects 0.000 claims 4
- 238000005382 thermal cycling Methods 0.000 claims 4
- 208000035657 Abasia Diseases 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 238000007792 addition Methods 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 238000010348 incorporation Methods 0.000 claims 2
- 125000006850 spacer group Chemical group 0.000 claims 2
- 230000007067 DNA methylation Effects 0.000 claims 1
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 239000005546 dideoxynucleotide Substances 0.000 claims 1
- 230000008034 disappearance Effects 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- -1 nucleotide amines Chemical class 0.000 claims 1
- 230000037452 priming Effects 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2007905445A AU2007905445A0 (en) | 2007-10-04 | Nucleic acid amplification | |
| AU2007905445 | 2007-10-04 | ||
| PCT/AU2008/001475 WO2009043112A1 (en) | 2007-10-04 | 2008-10-03 | Nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010539956A JP2010539956A (ja) | 2010-12-24 |
| JP2010539956A5 true JP2010539956A5 (enExample) | 2011-11-17 |
| JP5535921B2 JP5535921B2 (ja) | 2014-07-02 |
Family
ID=40525777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010527295A Active JP5535921B2 (ja) | 2007-10-04 | 2008-10-03 | 核酸増幅 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8455197B2 (enExample) |
| EP (1) | EP2207896B1 (enExample) |
| JP (1) | JP5535921B2 (enExample) |
| CN (1) | CN101889096B (enExample) |
| AU (1) | AU2008307153B2 (enExample) |
| ES (1) | ES2460896T3 (enExample) |
| WO (1) | WO2009043112A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009223671B2 (en) * | 2008-03-11 | 2014-11-27 | Sequenom, Inc. | Nucleic acid-based tests for prenatal gender determination |
| US8962247B2 (en) | 2008-09-16 | 2015-02-24 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses |
| US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| WO2011009073A1 (en) * | 2009-07-16 | 2011-01-20 | Abbott Laboratories | A method for amplification of nucleic acids |
| EP3660165B1 (en) | 2009-12-22 | 2023-01-04 | Sequenom, Inc. | Processes and kits for identifying aneuploidy |
| EP2702168B1 (en) | 2011-04-29 | 2018-01-17 | Sequenom, Inc. | Quantification of a minority nucleic acid species |
| AU2012340118A1 (en) * | 2011-11-17 | 2014-04-24 | Rheonix, Inc. | System and methods for selective molecular analysis |
| EP3757210B1 (en) | 2012-03-02 | 2022-08-24 | Sequenom, Inc. | Methods for enriching cancer nucleic acid from a biological sample |
| CA2872867C (en) | 2012-05-11 | 2020-09-15 | Clinical Genomics Pty Ltd | Diagnostic gene marker panel for colorectal cancer |
| US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| WO2014011928A1 (en) | 2012-07-13 | 2014-01-16 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| CA2893817C (en) * | 2012-12-13 | 2021-03-09 | Synthetic Genomics, Inc. | Peg-mediated assembly of nucleic acid molecules |
| EP3597774A1 (en) | 2013-03-13 | 2020-01-22 | Sequenom, Inc. | Primers for dna methylation analysis |
| EP2971134B1 (en) * | 2013-03-15 | 2023-10-25 | Aegea Biotechnologies, Inc. | Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence |
| EP2971124B1 (en) * | 2013-03-15 | 2019-12-25 | Aegea Biotechnologies, Inc. | Methods for amplification of nucleic acids utilizing clamp oligonuleotides |
| CN105555971B (zh) * | 2013-08-09 | 2017-08-29 | 卢米耐克斯公司 | 用于在核酸测定中改善熔链分辨和多重性的探针 |
| US11365447B2 (en) | 2014-03-13 | 2022-06-21 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| KR102343605B1 (ko) * | 2014-05-19 | 2021-12-27 | 윌리엄 마쉬 라이스 유니버시티 | 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭 |
| CN104131070A (zh) * | 2014-05-22 | 2014-11-05 | 苏州工业园区为真生物医药科技有限公司 | 一种对基因甲基化检测的方法 |
| EP3209801B1 (en) | 2014-10-20 | 2023-06-28 | Commonwealth Scientific and Industrial Research Organisation | Genome methylation analysis |
| CN105331727B (zh) * | 2015-12-01 | 2019-09-03 | 湖南宏雅基因技术有限公司 | 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒 |
| GB201612011D0 (en) * | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| ES3008759T3 (en) * | 2016-08-02 | 2025-03-25 | Hoffmann La Roche | Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids |
| DE102016120124B8 (de) * | 2016-10-21 | 2018-08-23 | Gna Biosolutions Gmbh | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
| US10787699B2 (en) * | 2017-02-08 | 2020-09-29 | Microsoft Technology Licensing, Llc | Generating pluralities of primer and payload designs for retrieval of stored nucleotides |
| EP3530753A1 (de) * | 2018-02-26 | 2019-08-28 | AGCT GmbH | Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität |
| WO2019162529A1 (de) * | 2018-02-26 | 2019-08-29 | Agct Gmbh | Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität |
| EP3530754A1 (de) * | 2018-02-26 | 2019-08-28 | AGCT GmbH | Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität |
| CN112074612A (zh) * | 2018-02-26 | 2020-12-11 | Agct有限公司 | 一种具有更高特异性的核酸扩增方法 |
| EP3759246A1 (de) * | 2018-02-28 | 2021-01-06 | AGCT GmbH | Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität |
| CN114075595B (zh) * | 2021-11-22 | 2023-11-17 | 上海交通大学 | 甲基化检测组合物、试剂盒及方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6335184B1 (en) * | 1993-07-23 | 2002-01-01 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
| US6057134A (en) * | 1996-10-07 | 2000-05-02 | Ambion, Inc. | Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides |
| ATE280177T1 (de) * | 1997-03-20 | 2004-11-15 | Hoffmann La Roche | Modifizierte primer |
| US7205129B1 (en) * | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
| TWI257426B (en) * | 2001-08-20 | 2006-07-01 | Takara Bio Inc | Nucleic acid amplification methods |
| US7629152B2 (en) * | 2002-03-01 | 2009-12-08 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
| EP1481088B1 (en) * | 2002-03-01 | 2008-09-17 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US20050153333A1 (en) | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| US20060014183A1 (en) * | 2004-06-10 | 2006-01-19 | Pfundheller Henrik M | Extendable probes |
| CN101443458B (zh) * | 2006-03-28 | 2014-04-02 | 联邦科学及工业研究组织 | 扩增dna片段 |
| WO2008064687A1 (en) * | 2006-11-27 | 2008-06-05 | Fluimedix | Fidelity enhanced allele specific amplification method |
-
2008
- 2008-10-03 JP JP2010527295A patent/JP5535921B2/ja active Active
- 2008-10-03 AU AU2008307153A patent/AU2008307153B2/en not_active Ceased
- 2008-10-03 WO PCT/AU2008/001475 patent/WO2009043112A1/en not_active Ceased
- 2008-10-03 ES ES08800110.2T patent/ES2460896T3/es active Active
- 2008-10-03 CN CN200880119335.8A patent/CN101889096B/zh not_active Expired - Fee Related
- 2008-10-03 EP EP08800110.2A patent/EP2207896B1/en active Active
- 2008-10-03 US US12/681,603 patent/US8455197B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
| US12371731B2 (en) | 2016-09-06 | 2025-07-29 | Integrated Dna Technologies, Inc. | Normalization of NGS library concentration |
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