JP2009528070A - 膵臓癌に関連するマイクロｒｎａ発現プロファイル - Google Patents
膵臓癌に関連するマイクロｒｎａ発現プロファイル Download PDFInfo
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| JP2015107091A (ja) * | 2013-12-05 | 2015-06-11 | 東レ株式会社 | 膵臓がんの検出キット及び検出方法 |
| JP2016123340A (ja) * | 2014-12-26 | 2016-07-11 | 株式会社エバンス | 膵癌治療感受性の診断方法及び膵癌治療感受性の増強剤 |
| KR20170010856A (ko) * | 2014-05-30 | 2017-02-01 | 도레이 카부시키가이샤 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| WO2019117269A1 (ja) | 2017-12-13 | 2019-06-20 | 国立大学法人広島大学 | 膵がんの検出を補助する方法 |
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| WO2010054386A2 (en) * | 2008-11-10 | 2010-05-14 | Battelle Memorial Institute | Methods, compositions, and devices utilizing microrna to determine physiological conditions |
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| JP2013542192A (ja) * | 2010-09-27 | 2013-11-21 | サバンチ ユニバーシテシ | マクロオートファジーの異常を伴う疾患の診断、予防、治療及びフォローアップのための、ｍｉＲＮＡの使用 |
| EP2474617A1 (en) | 2011-01-11 | 2012-07-11 | InteRNA Technologies BV | Mir for treating neo-angiogenesis |
| WO2013040251A2 (en) | 2011-09-13 | 2013-03-21 | Asurgen, Inc. | Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease |
| US9850541B2 (en) | 2011-12-19 | 2017-12-26 | Valley Health System | Methods and kits for detecting subjects at risk of having cancer |
| WO2013101783A2 (en) | 2011-12-30 | 2013-07-04 | Bio-Rad Laboratories, Inc. | Methods and compositions for performing nucleic acid amplification reactions |
| WO2013170146A1 (en) | 2012-05-10 | 2013-11-14 | Uab Research Foundation | Methods and compositions for modulating mir-204 activity |
| US9163235B2 (en) | 2012-06-21 | 2015-10-20 | MiRagen Therapeutics, Inc. | Inhibitors of the miR-15 family of micro-RNAs |
| US20150344961A1 (en) * | 2012-09-11 | 2015-12-03 | New York University | Sera Based miRNAs as Non-Invasive Biomarkers in Melanoma |
| WO2014093924A1 (en) | 2012-12-13 | 2014-06-19 | Moderna Therapeutics, Inc. | Modified nucleic acid molecules and uses thereof |
| CA2892529C (en) | 2012-11-26 | 2023-04-25 | Moderna Therapeutics, Inc. | Terminally modified rna |
| HK1217215A1 (zh) | 2013-01-17 | 2016-12-30 | Modernatx, Inc. | 用於改变细胞表型的信号传感器多核苷酸 |
| KR101501562B1 (ko) * | 2013-04-05 | 2015-03-11 | 연세대학교 산학협력단 | 췌장암 진단 마커를 검출하는 방법 |
| US20150018230A1 (en) * | 2013-06-11 | 2015-01-15 | Indiana University Research And Technology Corp. | PLASMA miRNA SIGNATURE FOR THE ACCURATE DIAGNOSIS OF PANCREATIC DUCTAL ADENOCARCINOMA |
| CA2939343A1 (en) | 2014-02-18 | 2015-08-27 | Baylor Research Institute | Mir-320e and colorectal cancer |
| US9909190B2 (en) | 2014-03-04 | 2018-03-06 | Hiroshima University | Method for assisting detection of pancreatic cancer |
| US9623040B2 (en) | 2014-07-14 | 2017-04-18 | The Board Of Trustees Of The Leland Stanford Junior University | Immunomodulation by controlling expression levels of microRNAs in dendritic cells |
| EP3041948B1 (en) | 2014-11-10 | 2019-01-09 | Modernatx, Inc. | Alternative nucleic acid molecules containing reduced uracil content and uses thereof |
| WO2017127750A1 (en) | 2016-01-22 | 2017-07-27 | Modernatx, Inc. | Messenger ribonucleic acids for the production of intracellular binding polypeptides and methods of use thereof |
| WO2017180587A2 (en) | 2016-04-11 | 2017-10-19 | Obsidian Therapeutics, Inc. | Regulated biocircuit systems |
| KR102469450B1 (ko) | 2016-05-18 | 2022-11-22 | 모더나티엑스, 인크. | 인터류킨-12 (il12)를 코딩하는 폴리뉴클레오티드 및 그의 용도 |
| AU2017286606A1 (en) | 2016-06-14 | 2018-12-13 | Modernatx, Inc. | Stabilized formulations of lipid nanoparticles |
| CN109661228A (zh) * | 2016-06-20 | 2019-04-19 | 库拉肿瘤学公司 | 用erk抑制剂治疗鳞状细胞癌 |
| US11427838B2 (en) | 2016-06-29 | 2022-08-30 | Vertex Pharmaceuticals Incorporated | Materials and methods for treatment of myotonic dystrophy type 1 (DM1) and other related disorders |
| EP4484443A3 (en) | 2016-06-29 | 2025-03-26 | CRISPR Therapeutics AG | Materials and methods for treatment of friedreich ataxia and other related disorders |
| WO2018002762A1 (en) | 2016-06-29 | 2018-01-04 | Crispr Therapeutics Ag | Materials and methods for treatment of amyotrophic lateral sclerosis (als) and other related disorders |
| CA3029132A1 (en) | 2016-07-06 | 2018-01-11 | Crispr Therapeutics Ag | Materials and methods for treatment of pain related disorders |
| JP7548696B2 (ja) | 2016-07-06 | 2024-09-10 | バーテックス ファーマシューティカルズ インコーポレイテッド | 疼痛関連障害を処置するための物質及び方法 |
| RU2765874C2 (ru) | 2016-10-26 | 2022-02-04 | МОДЕРНАТиЭкс, ИНК. | Матричные рибонуклеиновые кислоты для усиления иммунных ответов и способы их применения |
| US11584932B2 (en) | 2016-11-01 | 2023-02-21 | The Research Foundation For The State University Of New York | 5-halouracil-modified microRNAs and their use in the treatment of cancer |
| US11583504B2 (en) | 2016-11-08 | 2023-02-21 | Modernatx, Inc. | Stabilized formulations of lipid nanoparticles |
| EP3576780A1 (en) | 2017-02-01 | 2019-12-11 | Modernatx, Inc. | Immunomodulatory therapeutic mrna compositions encoding activating oncogene mutation peptides |
| US11559588B2 (en) | 2017-02-22 | 2023-01-24 | Crispr Therapeutics Ag | Materials and methods for treatment of Spinocerebellar Ataxia Type 1 (SCA1) and other Spinocerebellar Ataxia Type 1 Protein (ATXN1) gene related conditions or disorders |
| US11920148B2 (en) | 2017-02-22 | 2024-03-05 | Crispr Therapeutics Ag | Compositions and methods for gene editing |
| WO2018154459A1 (en) | 2017-02-22 | 2018-08-30 | Crispr Therapeutics Ag | Materials and methods for treatment of primary hyperoxaluria type 1 (ph1) and other alanine-glyoxylate aminotransferase (agxt) gene related conditions or disorders |
| EP3585807A1 (en) | 2017-02-22 | 2020-01-01 | CRISPR Therapeutics AG | Materials and methods for treatment of early onset parkinson's disease (park1) and other synuclein, alpha (snca) gene related conditions or disorders |
| US20200216857A1 (en) | 2017-02-22 | 2020-07-09 | Crispr Therapeutics Ag | Materials and methods for treatment of spinocerebellar ataxia type 2 (sca2) and other spinocerebellar ataxia type 2 protein (atxn2) gene related conditions or disorders |
| US20200131498A1 (en) | 2017-06-14 | 2020-04-30 | Modernatx, Inc. | Polynucleotides encoding methylmalonyl-coa mutase |
| US10636512B2 (en) | 2017-07-14 | 2020-04-28 | Cofactor Genomics, Inc. | Immuno-oncology applications using next generation sequencing |
| MX2020002348A (es) | 2017-08-31 | 2020-10-08 | Modernatx Inc | Métodos de elaboración de nanopartículas lipídicas. |
| US20190153440A1 (en) | 2017-11-21 | 2019-05-23 | Casebia Therapeutics Llp | Materials and methods for treatment of autosomal dominant retinitis pigmentosa |
| CA3084632A1 (en) | 2017-12-21 | 2019-06-27 | Crispr Therapeutics Ag | Materials and methods for treatment of usher syndrome type 2a |
| WO2019152557A1 (en) | 2018-01-30 | 2019-08-08 | Modernatx, Inc. | Compositions and methods for delivery of agents to immune cells |
| WO2019200171A1 (en) | 2018-04-11 | 2019-10-17 | Modernatx, Inc. | Messenger rna comprising functional rna elements |
| WO2019241315A1 (en) | 2018-06-12 | 2019-12-19 | Obsidian Therapeutics, Inc. | Pde5 derived regulatory constructs and methods of use in immunotherapy |
| US12090235B2 (en) | 2018-09-20 | 2024-09-17 | Modernatx, Inc. | Preparation of lipid nanoparticles and methods of administration thereof |
| WO2020086742A1 (en) | 2018-10-24 | 2020-04-30 | Obsidian Therapeutics, Inc. | Er tunable protein regulation |
| BR112021014845A2 (pt) | 2019-01-31 | 2021-11-03 | Modernatx Inc | Métodos de preparação de nanopartículas lipídicas |
| KR20210149251A (ko) | 2019-03-08 | 2021-12-08 | 옵시디안 테라퓨틱스, 인크. | 조율가능한 조절을 위한 인간 탄산 무수화효소 2 조성물 및 방법 |
| JP7418024B2 (ja) * | 2019-04-26 | 2024-01-19 | 国立大学法人大阪大学 | トンネル電流を使用したマイクロｒｎａ解析 |
| MA56539A (fr) | 2019-06-24 | 2022-04-27 | Modernatx Inc | Arn messager résistant à l'endonucléase et utilisations correspondantes |
| US20220387628A1 (en) | 2019-06-24 | 2022-12-08 | Modernatx, Inc. | Messenger rna comprising functional rna elements and uses thereof |
| US20220348937A1 (en) | 2019-09-06 | 2022-11-03 | Obsidian Therapeutics, Inc. | Compositions and methods for dhfr tunable protein regulation |
| JP2023513043A (ja) | 2020-01-31 | 2023-03-30 | モデルナティエックス インコーポレイテッド | 脂質ナノ粒子を調製する方法 |
| US11965162B2 (en) * | 2020-04-16 | 2024-04-23 | The Johns Hopkins University | MicroRNA and inhibitors thereof and methods of treatment |
| WO2021262919A2 (en) | 2020-06-26 | 2021-12-30 | The Research Foundation For The State University Of New York | 5-halouracil-modified micrornas and their use in the treatment of cancer |
| CN116710079A (zh) | 2020-07-24 | 2023-09-05 | 斯特兰德生物科技公司 | 包含经修饰的核苷酸的脂质纳米颗粒 |
| KR20230167008A (ko) | 2020-08-06 | 2023-12-07 | 모더나티엑스, 인크. | 지질 나노입자의 제조 방법 |
| US20240166707A1 (en) | 2021-01-08 | 2024-05-23 | Strand Therapeutics Inc. | Expression constructs and uses thereof |
| WO2022233880A1 (en) | 2021-05-03 | 2022-11-10 | Curevac Ag | Improved nucleic acid sequence for cell type specific expression |
| AU2023227443A1 (en) | 2022-03-01 | 2024-10-10 | Crispr Therapeutics Ag | Methods and compositions for treating angiopoietin-like 3 (angptl3) related conditions |
| JP2025516226A (ja) | 2022-04-26 | 2025-05-27 | ストランド セラピューティクス インコーポレイテッド | ベネズエラウマ脳炎（ｖｅｅ）レプリコンを含む脂質ナノ粒子及びその使用 |
| EP4561546A1 (en) | 2022-07-29 | 2025-06-04 | ModernaTX, Inc. | Lipid nanoparticle compositions comprising surface lipid derivatives and related uses |
| WO2024026475A1 (en) | 2022-07-29 | 2024-02-01 | Modernatx, Inc. | Compositions for delivery to hematopoietic stem and progenitor cells (hspcs) and related uses |
| EP4561547A1 (en) | 2022-07-29 | 2025-06-04 | ModernaTX, Inc. | Lipid nanoparticle compositions comprising phospholipid derivatives and related uses |
| CN121079317A (zh) | 2023-04-28 | 2025-12-05 | 深圳深信生物科技有限公司 | 经修饰的递送载体及其应用 |
| WO2024259373A1 (en) | 2023-06-14 | 2024-12-19 | Modernatx, Inc. | Compounds and compositions for delivery of therapeutic agents |
| WO2025160381A1 (en) | 2024-01-26 | 2025-07-31 | Modernatx, Inc. | Methods of preparing lipid nanoparticles |
| WO2025166202A1 (en) | 2024-01-31 | 2025-08-07 | Modernatx, Inc. | Lipid nanoparticle compositions comprising sialic acid derivatives and the uses thereof |
| CN119391860B (zh) * | 2024-12-25 | 2025-03-21 | 慧算基因科技(上海)有限公司 | 一种用于胰腺癌术后mrd检测及动态监测的引物探针组合物、试剂盒及应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005078139A2 (en) * | 2004-02-09 | 2005-08-25 | Thomas Jefferson University | DIAGNOSIS AND TREATMENT OF CANCERS WITH MicroRNA LOCATED IN OR NEAR CANCER-ASSOCIATED CHROMOSOMAL FEATURES |
| WO2005118806A2 (en) * | 2004-05-28 | 2005-12-15 | Ambion, Inc. | METHODS AND COMPOSITIONS INVOLVING MicroRNA |
| JP2009521952A (ja) * | 2006-01-05 | 2009-06-11 | ジ・オハイオ・ステイト・ユニバーシティ・リサーチ・ファウンデイション | 膵内分泌腫瘍及び膵腺房腫瘍におけるマイクロｒｎａ発現異常 |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1990A (en) | 1841-02-20 | Head-block of sawmills for sawing without stub-shot and gaging the | ||
| US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
| US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
| US5019369A (en) | 1984-10-22 | 1991-05-28 | Vestar, Inc. | Method of targeting tumors in humans |
| US5139941A (en) | 1985-10-31 | 1992-08-18 | University Of Florida Research Foundation, Inc. | AAV transduction vectors |
| US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
| US4920016A (en) | 1986-12-24 | 1990-04-24 | Linear Technology, Inc. | Liposomes with enhanced circulation time |
| ATE120497T1 (de) | 1988-05-09 | 1995-04-15 | Univ Temple | Verfahren zur voraussage der wirksamkeit einer antineoplastichen behandlung bei einzelnen patienten. |
| AU647741B2 (en) | 1989-12-01 | 1994-03-31 | Regents Of The University Of California, The | Methods and compositions for chromosome-specific staining |
| US5252479A (en) | 1991-11-08 | 1993-10-12 | Research Corporation Technologies, Inc. | Safe vector for gene therapy |
| US5587308A (en) | 1992-06-02 | 1996-12-24 | The United States Of America As Represented By The Department Of Health & Human Services | Modified adeno-associated virus vector capable of expression from a novel promoter |
| US5478745A (en) | 1992-12-04 | 1995-12-26 | University Of Pittsburgh | Recombinant viral vector system |
| US5849902A (en) | 1996-09-26 | 1998-12-15 | Oligos Etc. Inc. | Three component chimeric antisense oligonucleotides |
| US6232068B1 (en) | 1999-01-22 | 2001-05-15 | Rosetta Inpharmatics, Inc. | Monitoring of gene expression by detecting hybridization to nucleic acid arrays using anti-heteronucleic acid antibodies |
| EP1309726B2 (en) | 2000-03-30 | 2018-10-03 | Whitehead Institute For Biomedical Research | Rna sequence-specific mediators of rna interference |
| US20020173478A1 (en) | 2000-11-14 | 2002-11-21 | The Trustees Of The University Of Pennsylvania | Post-transcriptional gene silencing by RNAi in mammalian cells |
| US20030138793A1 (en) * | 2001-06-10 | 2003-07-24 | Irm Llc, A Delaware Limited Liability Company | Molecular signatures of commonly fatal carcinomas |
| EP2390328A1 (en) * | 2001-09-28 | 2011-11-30 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | MicroRNA molecules |
| US8119610B2 (en) | 2002-05-31 | 2012-02-21 | The Regents Of The University Of California | Method for efficient RNA interference in mammalian cells |
| US7148342B2 (en) | 2002-07-24 | 2006-12-12 | The Trustees Of The University Of Pennyslvania | Compositions and methods for sirna inhibition of angiogenesis |
| EP1534490B1 (en) | 2002-08-21 | 2006-08-02 | Mihaly Rigo | equipment for manufacturing pipe-couplings and pipe couplings |
| US20050260639A1 (en) * | 2002-09-30 | 2005-11-24 | Oncotherapy Science, Inc. | Method for diagnosing pancreatic cancer |
| US20040152112A1 (en) * | 2002-11-13 | 2004-08-05 | Thomas Jefferson University | Compositions and methods for cancer diagnosis and therapy |
| US7906326B2 (en) * | 2003-05-07 | 2011-03-15 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory oligonucleotides associated with alzheimer's disease and uses thereof |
| US7250496B2 (en) * | 2002-11-14 | 2007-07-31 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory genes and uses thereof |
| US8106180B2 (en) * | 2003-08-07 | 2012-01-31 | Whitehead Institute For Biomedical Research | Methods and products for expression of micro RNAs |
| US20050142581A1 (en) * | 2003-09-04 | 2005-06-30 | Griffey Richard H. | Microrna as ligands and target molecules |
| US20050182005A1 (en) * | 2004-02-13 | 2005-08-18 | Tuschl Thomas H. | Anti-microRNA oligonucleotide molecules |
| US7365058B2 (en) * | 2004-04-13 | 2008-04-29 | The Rockefeller University | MicroRNA and methods for inhibiting same |
| US20050277139A1 (en) | 2004-04-26 | 2005-12-15 | Itzhak Bentwich | Methods and apparatus for the detection and validation of microRNAs |
| US7635563B2 (en) * | 2004-06-30 | 2009-12-22 | Massachusetts Institute Of Technology | High throughput methods relating to microRNA expression analysis |
| DE602005025782D1 (de) | 2004-09-21 | 2011-02-17 | Life Technologies Corp | Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) |
| GB2480980A (en) * | 2009-03-02 | 2011-12-07 | Trackfive Diagnostics Inc | Methods for predicting cancer response to EGFR inhibitors |
| US20130310276A1 (en) * | 2010-12-22 | 2013-11-21 | Ruprecht-Karls University of Heidelberg | Microrna for diagnosis of pancreatic cancer |
| WO2013040251A2 (en) * | 2011-09-13 | 2013-03-21 | Asurgen, Inc. | Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease |
| US20140100124A1 (en) * | 2012-10-04 | 2014-04-10 | Asuragen, Inc. | Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions |
-
2007
- 2007-03-02 EP EP12168652A patent/EP2522747A1/en not_active Withdrawn
- 2007-03-02 JP JP2008557519A patent/JP2009528070A/ja active Pending
- 2007-03-02 EP EP12168648.9A patent/EP2522746B1/en not_active Ceased
- 2007-03-02 WO PCT/US2007/063208 patent/WO2007103808A2/en not_active Ceased
- 2007-03-02 ES ES07757822.7T patent/ES2448491T3/es active Active
- 2007-03-02 EP EP12168656A patent/EP2522750A1/en not_active Withdrawn
- 2007-03-02 AU AU2007223311A patent/AU2007223311C1/en not_active Ceased
- 2007-03-02 EP EP12168654A patent/EP2522748A1/en not_active Withdrawn
- 2007-03-02 CA CA2638844A patent/CA2638844C/en not_active Expired - Fee Related
- 2007-03-02 EP EP07757822.7A patent/EP1991706B1/en not_active Not-in-force
- 2007-03-02 US US12/281,194 patent/US20100286232A1/en not_active Abandoned
- 2007-03-02 EP EP12168655A patent/EP2522749A1/en not_active Withdrawn
-
2010
- 2010-12-07 US US12/962,065 patent/US20110171646A1/en not_active Abandoned
-
2012
- 2012-08-29 JP JP2012188756A patent/JP5841024B2/ja not_active Expired - Fee Related
-
2013
- 2013-02-08 JP JP2013023199A patent/JP2013081483A/ja active Pending
-
2014
- 2014-04-03 US US14/244,201 patent/US20140357702A1/en not_active Abandoned
-
2017
- 2017-09-05 US US15/695,477 patent/US20180105865A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005078139A2 (en) * | 2004-02-09 | 2005-08-25 | Thomas Jefferson University | DIAGNOSIS AND TREATMENT OF CANCERS WITH MicroRNA LOCATED IN OR NEAR CANCER-ASSOCIATED CHROMOSOMAL FEATURES |
| WO2005118806A2 (en) * | 2004-05-28 | 2005-12-15 | Ambion, Inc. | METHODS AND COMPOSITIONS INVOLVING MicroRNA |
| JP2009521952A (ja) * | 2006-01-05 | 2009-06-11 | ジ・オハイオ・ステイト・ユニバーシティ・リサーチ・ファウンデイション | 膵内分泌腫瘍及び膵腺房腫瘍におけるマイクロｒｎａ発現異常 |
Non-Patent Citations (1)
| Title |
|---|
| JPN6012027598; Ｐｒｏｃ． Ｎａｔｌ． Ａｃａｄ． Ｓｃｉ． ＵＳＡ Ｖｏｌ．１０３， Ｎｏ．７, 20060214, ｐ．２２５７-２２６１ * |
Cited By (28)
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|---|---|---|---|---|
| JP2020120674A (ja) * | 2012-12-06 | 2020-08-13 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ． ＬＬＣ | Ｃｒｉｓｐｒに基づくゲノム修飾および制御 |
| JP7478772B2 (ja) | 2012-12-06 | 2024-05-07 | シグマ－アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | Ｃｒｉｓｐｒに基づくゲノム修飾および制御 |
| JP2022115994A (ja) * | 2012-12-06 | 2022-08-09 | シグマ－アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | Ｃｒｉｓｐｒに基づくゲノム修飾および制御 |
| JP2015107091A (ja) * | 2013-12-05 | 2015-06-11 | 東レ株式会社 | 膵臓がんの検出キット及び検出方法 |
| JP2024075722A (ja) * | 2014-05-30 | 2024-06-04 | 東レ株式会社 | 膵臓がんの検出キット又はデバイス及び検出方法 |
| KR102594868B1 (ko) | 2014-05-30 | 2023-10-30 | 국립연구개발법인 고쿠리츠간켄큐센터 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
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| JP7687638B2 (ja) | 2014-05-30 | 2025-06-03 | 東レ株式会社 | 膵臓がんの検出キット又はデバイス及び検出方法 |
| US12188098B2 (en) | 2014-05-30 | 2025-01-07 | Toray Industries, Inc. | Pancreatic cancer detection kit or device, and detection method |
| KR102747981B1 (ko) | 2014-05-30 | 2024-12-31 | 국립연구개발법인 고쿠리츠간켄큐센터 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| JP7082380B2 (ja) | 2014-05-30 | 2022-06-08 | 東レ株式会社 | 膵臓がんの検出キット又はデバイス及び検出方法 |
| JPWO2015182781A1 (ja) * | 2014-05-30 | 2017-04-20 | 東レ株式会社 | 膵臓がんの検出キット又はデバイス及び検出方法 |
| JP2022122899A (ja) * | 2014-05-30 | 2022-08-23 | 東レ株式会社 | 膵臓がんの検出キット又はデバイス及び検出方法 |
| KR20240105518A (ko) * | 2014-05-30 | 2024-07-05 | 도레이 카부시키가이샤 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| US11788150B2 (en) | 2014-05-30 | 2023-10-17 | Toray Industries, Inc. | Pancreatic cancer detection kit or device, and detection method |
| KR102680815B1 (ko) | 2014-05-30 | 2024-07-03 | 국립연구개발법인 고쿠리츠간켄큐센터 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| KR20230152169A (ko) * | 2014-05-30 | 2023-11-02 | 도레이 카부시키가이샤 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| KR102642854B1 (ko) | 2014-05-30 | 2024-03-04 | 국립연구개발법인 고쿠리츠간켄큐센터 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| KR20240034257A (ko) * | 2014-05-30 | 2024-03-13 | 도레이 카부시키가이샤 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| JP7466157B2 (ja) | 2014-05-30 | 2024-04-12 | 東レ株式会社 | 膵臓がんの検出キット又はデバイス及び検出方法 |
| KR20170010856A (ko) * | 2014-05-30 | 2017-02-01 | 도레이 카부시키가이샤 | 췌장암의 검출 키트 또는 디바이스 및 검출 방법 |
| JP2016123340A (ja) * | 2014-12-26 | 2016-07-11 | 株式会社エバンス | 膵癌治療感受性の診断方法及び膵癌治療感受性の増強剤 |
| US12098430B2 (en) | 2016-03-31 | 2024-09-24 | Toray Industries, Inc. | Kit or device for detecting early stage pancreatic cancer or pancreatic cancer precursor lesions and detection method therefor |
| WO2019117269A1 (ja) | 2017-12-13 | 2019-06-20 | 国立大学法人広島大学 | 膵がんの検出を補助する方法 |
| JP7298914B2 (ja) | 2017-12-13 | 2023-06-27 | 国立大学法人広島大学 | 膵がんの検出を補助する方法 |
| JPWO2019117269A1 (ja) * | 2017-12-13 | 2021-02-18 | 国立大学法人広島大学 | 膵がんの検出を補助する方法 |
| JPWO2022014670A1 (enExample) * | 2020-07-16 | 2022-01-20 | ||
| WO2022014670A1 (ja) * | 2020-07-16 | 2022-01-20 | 学校法人 久留米大学 | 膵臓がんのバイオマーカーとしてのマイクロｒｎａの使用 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013081483A (ja) | 2013-05-09 |
| EP2522749A1 (en) | 2012-11-14 |
| ES2448491T3 (es) | 2014-03-14 |
| US20110171646A1 (en) | 2011-07-14 |
| EP1991706A4 (en) | 2010-03-17 |
| CA2638844C (en) | 2016-05-03 |
| JP2012231801A (ja) | 2012-11-29 |
| EP2522747A1 (en) | 2012-11-14 |
| WO2007103808A3 (en) | 2008-02-28 |
| EP1991706B1 (en) | 2014-01-15 |
| AU2007223311A1 (en) | 2007-09-13 |
| US20180105865A1 (en) | 2018-04-19 |
| US20140357702A1 (en) | 2014-12-04 |
| EP2522746B1 (en) | 2014-11-12 |
| EP2522750A1 (en) | 2012-11-14 |
| JP5841024B2 (ja) | 2016-01-06 |
| US20100286232A1 (en) | 2010-11-11 |
| AU2007223311B2 (en) | 2012-12-13 |
| EP1991706A2 (en) | 2008-11-19 |
| AU2007223311C1 (en) | 2013-06-20 |
| EP2522748A1 (en) | 2012-11-14 |
| CA2638844A1 (en) | 2007-09-13 |
| WO2007103808A2 (en) | 2007-09-13 |
| EP2522746A1 (en) | 2012-11-14 |
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