JP2009509565A5 - - Google Patents

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JP2009509565A5
JP2009509565A5 JP2008534568A JP2008534568A JP2009509565A5 JP 2009509565 A5 JP2009509565 A5 JP 2009509565A5 JP 2008534568 A JP2008534568 A JP 2008534568A JP 2008534568 A JP2008534568 A JP 2008534568A JP 2009509565 A5 JP2009509565 A5 JP 2009509565A5
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region
quencher
inhibitor
nucleotide sequence
nucleotide
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JP2008534568A 2005-10-03 2006-09-29 核酸を増幅するための組成物、方法およびキット Active JP5438320B2 (ja)

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US72338305P 2005-10-03 2005-10-03
US60/723,383 2005-10-03
PCT/US2006/037829 WO2007041201A2 (en) 2005-10-03 2006-09-29 Compositions, methods, and kits for amplifying nucleic acids

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JP2012181035A Division JP5890762B2 (ja) 2005-10-03 2012-08-17 核酸を増幅するための組成物、方法およびキット

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JP2009509565A JP2009509565A (ja) 2009-03-12
JP2009509565A5 true JP2009509565A5 (enExample) 2012-10-04
JP5438320B2 JP5438320B2 (ja) 2014-03-12

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JP2008534568A Active JP5438320B2 (ja) 2005-10-03 2006-09-29 核酸を増幅するための組成物、方法およびキット
JP2012181035A Active JP5890762B2 (ja) 2005-10-03 2012-08-17 核酸を増幅するための組成物、方法およびキット
JP2015127341A Active JP6157541B2 (ja) 2005-10-03 2015-06-25 核酸を増幅するための組成物、方法およびキット
JP2016162389A Withdrawn JP2016192979A (ja) 2005-10-03 2016-08-23 核酸を増幅するための組成物、方法およびキット
JP2018083860A Active JP7274264B2 (ja) 2005-10-03 2018-04-25 核酸を増幅するための組成物、方法およびキット
JP2019131946A Withdrawn JP2019193664A (ja) 2005-10-03 2019-07-17 核酸を増幅するための組成物、方法およびキット
JP2021137897A Pending JP2021182939A (ja) 2005-10-03 2021-08-26 核酸を増幅するための組成物、方法およびキット
JP2023031224A Pending JP2023065578A (ja) 2005-10-03 2023-03-01 核酸を増幅するための組成物、方法およびキット
JP2023135399A Pending JP2023156506A (ja) 2005-10-03 2023-08-23 核酸を増幅するための組成物、方法およびキット

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JP2012181035A Active JP5890762B2 (ja) 2005-10-03 2012-08-17 核酸を増幅するための組成物、方法およびキット
JP2015127341A Active JP6157541B2 (ja) 2005-10-03 2015-06-25 核酸を増幅するための組成物、方法およびキット
JP2016162389A Withdrawn JP2016192979A (ja) 2005-10-03 2016-08-23 核酸を増幅するための組成物、方法およびキット
JP2018083860A Active JP7274264B2 (ja) 2005-10-03 2018-04-25 核酸を増幅するための組成物、方法およびキット
JP2019131946A Withdrawn JP2019193664A (ja) 2005-10-03 2019-07-17 核酸を増幅するための組成物、方法およびキット
JP2021137897A Pending JP2021182939A (ja) 2005-10-03 2021-08-26 核酸を増幅するための組成物、方法およびキット
JP2023031224A Pending JP2023065578A (ja) 2005-10-03 2023-03-01 核酸を増幅するための組成物、方法およびキット
JP2023135399A Pending JP2023156506A (ja) 2005-10-03 2023-08-23 核酸を増幅するための組成物、方法およびキット

Country Status (6)

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US (6) US20070212704A1 (enExample)
EP (2) EP2458011B1 (enExample)
JP (9) JP5438320B2 (enExample)
CN (2) CN103215248B (enExample)
CA (1) CA2624634A1 (enExample)
WO (1) WO2007041201A2 (enExample)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
KR20030082535A (ko) 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Ｒｎａ 서열의 증폭을 위한 방법 및 조성물
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
KR20070090158A (ko) * 2004-10-18 2007-09-05 브랜데이스 유니버시티 Ｐｃｒ 증폭에서 재현성을 개선시키고 미스프라이밍을감소시키기 위한 시약 및 방법
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
GB2470672B (en) * 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
KR102231531B1 (ko) 2009-02-13 2021-03-23 엑스-켐, 인크. Ｄｎａ―코딩된 라이브러리의 생성 및 스크리닝 방법
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
US20120165202A1 (en) * 2009-04-30 2012-06-28 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) * 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
JP5977000B2 (ja) * 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
US9109226B2 (en) 2011-09-01 2015-08-18 New England Biolabs, Inc. Synthetic nucleic acids for polymerization reactions
ES2675111T3 (es) 2011-09-07 2018-07-06 X-Chem, Inc. Métodos para etiquetar bibliotecas con codificación de ADN
WO2013038534A1 (ja) * 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
SG11201400635UA (en) * 2011-09-14 2014-09-26 Ngk Insulators Ltd Method for detecting target nucleic acid
CN103998921B (zh) * 2011-10-14 2016-04-20 贝克顿·迪金森公司 方波热循环
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
EP2836603B1 (en) * 2012-04-12 2018-06-06 New England Biolabs, Inc. Synthetic nucleic acids for polymerization reactions
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
CN104685115A (zh) 2012-07-13 2015-06-03 X-化学有限公司 具有聚合酶不可读的编码性寡核苷酸连接的dna编码文库
HK1211323A1 (en) * 2012-07-23 2016-05-20 珠海圣美生物诊断技术有限公司 Dual enzymatic amplification
US10457978B2 (en) * 2012-08-17 2019-10-29 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cyclopentane-peptide nucleic acids for qualitative and quantitative detection of nucleic acids
US9410173B2 (en) 2012-10-24 2016-08-09 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
JP6480336B2 (ja) 2012-10-24 2019-03-06 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ ヒドロキサメート置換アザインドリン−シアニン染料およびそのバイオ複合体
JP2015050980A (ja) * 2013-09-09 2015-03-19 東洋紡株式会社 非特異増幅を低減させる方法
CA2923812C (en) 2013-10-17 2023-10-17 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
CN106458885B (zh) 2013-10-25 2019-12-10 生命技术公司 用于聚合酶链式反应系统的新颖化合物及其应用
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
EP3194629A4 (en) * 2014-09-17 2018-03-07 Theranos, Inc. Diagnostic methods and compositions
CN114507715B (zh) * 2014-11-20 2025-01-07 杭州智灵龙生物科技有限公司 用于核酸扩增的组合物和方法
EP3234186A1 (en) * 2014-12-19 2017-10-25 Brandeis University Mispriming prevention reagents
US10066259B2 (en) 2015-01-06 2018-09-04 Good Start Genetics, Inc. Screening for structural variants
CN113564168B (zh) * 2015-04-23 2024-10-25 美国杰龙生物医药公司 使用多价阳离子盐组合物制备多核苷酸的方法
ES2961374T3 (es) * 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
US10619189B2 (en) * 2015-05-11 2020-04-14 3M Innovative Properties Company Composition for reducing inhibition of nucleic acid amplification
WO2017172760A1 (en) * 2016-03-29 2017-10-05 William Marsh Rice University Surface-based detection of nucleic acid in a convection flow fluidic device
US11299739B2 (en) * 2016-06-07 2022-04-12 Cepheid Thermostable polymerase inhibitor compositions and methods
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
CN118207299A (zh) 2016-08-26 2024-06-18 生命技术公司 核酸提取和扩增对照及其使用方法
WO2018087200A1 (en) * 2016-11-11 2018-05-17 Roche Innovation Center Copenhagen A/S Therapeutic oligonucleotides capture and detection
US20180188204A1 (en) * 2017-01-01 2018-07-05 Sylvester Tumusiime Use of charged quinine sulfate or other precursors or derivatives of quinine alkaloids in visualization of nucleic acids
CN108546748A (zh) * 2017-04-05 2018-09-18 杭州丹威生物科技有限公司 一种检测核酸的方法和试剂盒
IL276343B2 (en) * 2018-01-29 2024-10-01 St Jude Childrens Res Hospital Inc Method for nucleic acid amplification
CN108588050B (zh) * 2018-05-14 2021-06-25 北京艾克伦医疗科技有限公司 Dna聚合酶以及核酸检测方法和试剂盒
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
AU2019403220A1 (en) * 2018-12-20 2021-07-15 Alveo Technologies, Inc. Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions
CN110628877B (zh) * 2019-05-17 2024-07-16 澳门大学 增强核酸扩增反应的增强剂、试剂盒和进行核酸扩增反应的方法
CN113528624B (zh) * 2020-04-17 2025-12-02 青岛简码基因科技有限公司 扩增和检测核酸的方法及试剂盒
CN115135664A (zh) * 2020-06-22 2022-09-30 苏州新海生物科技股份有限公司 一种核酸配体及其应用
US12122921B2 (en) * 2020-07-23 2024-10-22 Enzo Biochem, Inc. Fluorescence quencher polymer conjugates and uses thereof
CN111926067B (zh) * 2020-09-24 2021-01-08 圣湘生物科技股份有限公司 用于荧光定量pcr的双探针组合物、试剂盒、用途及方法
KR102661292B1 (ko) * 2021-09-09 2024-04-30 포항공과대학교 산학협력단 금속 이온 반응성 dna 중합 효소 스위치 및 이에 의해 제어되는 등온 증폭 방법
JP2025531820A (ja) * 2022-09-07 2025-09-25 ベクトン・ディキンソン・アンド・カンパニー 検出特異性改善のための修飾分子ビーコン
WO2024118922A1 (en) * 2022-12-01 2024-06-06 Becton, Dickinson And Company Asymmetric hairpin probes for nucleic acid detection

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US6004826A (en) 1988-07-20 1999-12-21 David Segev Repair-mediated process for amplifying and detecting nucleic acid sequences
US5763173A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5874557A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5693502A (en) * 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
ES2128323T3 (es) 1990-07-24 1999-05-16 Hoffmann La Roche Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas.
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5614402A (en) 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
CA2159830C (en) 1994-04-29 2001-07-03 Applied Biosystems, Llc System for real time detection of nucleic acid amplification products
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
WO1996041010A1 (en) * 1995-06-07 1996-12-19 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
US6183967B1 (en) 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
ATE428801T1 (de) 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6043060A (en) 1996-11-18 2000-03-28 Imanishi; Takeshi Nucleotide analogues
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US6143877A (en) 1997-04-30 2000-11-07 Epoch Pharmaceuticals, Inc. Oligonucleotides including pyrazolo[3,4-D]pyrimidine bases, bound in double stranded nucleic acids
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
CA2303299C (en) 1997-09-12 2016-02-23 Exiqon A/S Oligonucleotide analogues
US6127121A (en) 1998-04-03 2000-10-03 Epoch Pharmaceuticals, Inc. Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
DE69933770D1 (de) 1998-07-09 2006-12-07 Biocept Inc Verfahren zur verwendung einer universellen bibliothek von peptid-nukleinsäuren zur optimierung der dns-hybridisierung
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
AU782555B2 (en) * 1999-07-02 2005-08-11 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
CA2384838C (en) 1999-09-13 2006-07-18 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
JP3463098B2 (ja) * 1999-10-08 2003-11-05 独立行政法人産業技術総合研究所 モジュレートアプタマー及びこれを用いた標的タンパク質の検出方法
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US6579680B2 (en) * 2000-02-28 2003-06-17 Corning Incorporated Method for label-free detection of hybridized DNA targets
DE60141205D1 (de) * 2000-04-03 2010-03-18 Cytyc Corp Nachweis und typisierung des papillomavirus mittels pna-sonden
SE0001768D0 (sv) 2000-05-12 2000-05-12 Helen Andersson Mikrofluidisk flödescell för manipulering av partiklar
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
DE60125243T2 (de) 2000-07-03 2007-05-31 Applera Corp., Foster City Polynukleotid-sequenzassay
WO2002006827A1 (en) * 2000-07-05 2002-01-24 The General Hospital Corporation Compounds and methods for fluorescently labeling nucleic acids
US7309573B2 (en) * 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7267945B2 (en) 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US7582739B2 (en) 2002-03-11 2009-09-01 Epoch Biosciences, Inc. Negatively charged minor groove binders
US20050053942A1 (en) * 2002-06-24 2005-03-10 Sakari Kauppinen Methods and systems for detection and isolation of a nucleotide sequence
US20040132051A1 (en) 2002-07-26 2004-07-08 Andersen Mark R. Mg-mediated hot start biochemical reactions
EP3000899A1 (en) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplex amplification of polynucleotides
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
JP4567673B2 (ja) 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
CN1661359B (zh) * 2004-02-25 2013-06-12 陕西西大北美基因股份有限公司 一种核酸等温扩增定量检测方法
KR20070090158A (ko) * 2004-10-18 2007-09-05 브랜데이스 유니버시티 Ｐｃｒ 증폭에서 재현성을 개선시키고 미스프라이밍을감소시키기 위한 시약 및 방법
CN1308461C (zh) * 2004-12-24 2007-04-04 武汉大学 一种检测口蹄疫病毒的荧光定量pcr试剂盒及应用
US8530194B2 (en) * 2005-09-26 2013-09-10 Allelogic Biosciences Corporation Oligonucleotides as temperature-sensitive inhibitors for DNA polymerases
WO2007041501A2 (en) 2005-09-30 2007-04-12 Phoenix Technologies Ltd. Secure execution environment by preventing execution of unauthorized boot loaders
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids

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