JP5438320B2 - 核酸を増幅するための組成物、方法およびキット - Google Patents

核酸を増幅するための組成物、方法およびキット Download PDF

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JP5438320B2
JP5438320B2 JP2008534568A JP2008534568A JP5438320B2 JP 5438320 B2 JP5438320 B2 JP 5438320B2 JP 2008534568 A JP2008534568 A JP 2008534568A JP 2008534568 A JP2008534568 A JP 2008534568A JP 5438320 B2 JP5438320 B2 JP 5438320B2
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inhibitor
dna polymerase
enzyme
quencher
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JP2009509565A5 (enExample
JP2009509565A (ja
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シュリアン ドン，
ジュンコ スティーブンス，
ダニー エイチ． リー，
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アプライド バイオシステムズ リミテッド ライアビリティー カンパニー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

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JP2008534568A 2005-10-03 2006-09-29 核酸を増幅するための組成物、方法およびキット Active JP5438320B2 (ja)

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US72338305P 2005-10-03 2005-10-03
US60/723,383 2005-10-03
PCT/US2006/037829 WO2007041201A2 (en) 2005-10-03 2006-09-29 Compositions, methods, and kits for amplifying nucleic acids

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JP2015127341A Active JP6157541B2 (ja) 2005-10-03 2015-06-25 核酸を増幅するための組成物、方法およびキット
JP2016162389A Withdrawn JP2016192979A (ja) 2005-10-03 2016-08-23 核酸を増幅するための組成物、方法およびキット
JP2018083860A Active JP7274264B2 (ja) 2005-10-03 2018-04-25 核酸を増幅するための組成物、方法およびキット
JP2019131946A Withdrawn JP2019193664A (ja) 2005-10-03 2019-07-17 核酸を増幅するための組成物、方法およびキット
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JP2023031224A Pending JP2023065578A (ja) 2005-10-03 2023-03-01 核酸を増幅するための組成物、方法およびキット
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JP2016162389A Withdrawn JP2016192979A (ja) 2005-10-03 2016-08-23 核酸を増幅するための組成物、方法およびキット
JP2018083860A Active JP7274264B2 (ja) 2005-10-03 2018-04-25 核酸を増幅するための組成物、方法およびキット
JP2019131946A Withdrawn JP2019193664A (ja) 2005-10-03 2019-07-17 核酸を増幅するための組成物、方法およびキット
JP2021137897A Pending JP2021182939A (ja) 2005-10-03 2021-08-26 核酸を増幅するための組成物、方法およびキット
JP2023031224A Pending JP2023065578A (ja) 2005-10-03 2023-03-01 核酸を増幅するための組成物、方法およびキット
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US (6) US20070212704A1 (enExample)
EP (2) EP1943348B1 (enExample)
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CN (2) CN101321877B (enExample)
CA (1) CA2624634A1 (enExample)
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US20070212704A1 (en) 2007-09-13
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US11225686B2 (en) 2022-01-18

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