JP2008541761A5 - - Google Patents
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- Publication number
- JP2008541761A5 JP2008541761A5 JP2008514787A JP2008514787A JP2008541761A5 JP 2008541761 A5 JP2008541761 A5 JP 2008541761A5 JP 2008514787 A JP2008514787 A JP 2008514787A JP 2008514787 A JP2008514787 A JP 2008514787A JP 2008541761 A5 JP2008541761 A5 JP 2008541761A5
- Authority
- JP
- Japan
- Prior art keywords
- minutes
- warming
- paraffin
- aqueous phase
- incubation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 90
- 239000012188 paraffin wax Substances 0.000 claims 24
- 238000010792 warming Methods 0.000 claims 23
- 239000008346 aqueous phase Substances 0.000 claims 22
- 238000011534 incubation Methods 0.000 claims 20
- 238000000746 purification Methods 0.000 claims 15
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims 10
- 108091005804 Peptidases Proteins 0.000 claims 10
- 239000004365 Protease Substances 0.000 claims 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 10
- 239000002563 ionic surfactant Substances 0.000 claims 10
- 235000019419 proteases Nutrition 0.000 claims 10
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 claims 10
- 239000012071 phase Substances 0.000 claims 9
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 8
- 229920002477 rna polymer Polymers 0.000 claims 6
- 239000004094 surface-active agent Substances 0.000 claims 6
- 108010067770 Endopeptidase K Proteins 0.000 claims 5
- 108010077895 Sarcosine Proteins 0.000 claims 5
- 238000005571 anion exchange chromatography Methods 0.000 claims 5
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 claims 5
- 238000007899 nucleic acid hybridization Methods 0.000 claims 5
- 238000001556 precipitation Methods 0.000 claims 5
- 229940043230 sarcosine Drugs 0.000 claims 5
- 239000000377 silicon dioxide Substances 0.000 claims 5
- 102000053602 DNA Human genes 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 4
- 238000010438 heat treatment Methods 0.000 claims 4
- 239000002736 nonionic surfactant Substances 0.000 claims 4
- IDOQDZANRZQBTP-UHFFFAOYSA-N 2-[2-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical group CC(C)(C)CC(C)(C)C1=CC=CC=C1OCCO IDOQDZANRZQBTP-UHFFFAOYSA-N 0.000 claims 2
- 229920001213 Polysorbate 20 Polymers 0.000 claims 2
- 229920004929 Triton X-114 Polymers 0.000 claims 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68652205P | 2005-05-31 | 2005-05-31 | |
| US77302706P | 2006-02-13 | 2006-02-13 | |
| PCT/US2006/021011 WO2006130632A2 (en) | 2005-05-31 | 2006-05-31 | Separation and purification of nucleic acid from paraffin-containing samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008541761A JP2008541761A (ja) | 2008-11-27 |
| JP2008541761A5 true JP2008541761A5 (enExample) | 2010-09-24 |
Family
ID=37482243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008514787A Pending JP2008541761A (ja) | 2005-05-31 | 2006-05-31 | パラフィン含有試料からの核酸の分離及び精製 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US9243241B2 (enExample) |
| EP (1) | EP1910389A4 (enExample) |
| JP (1) | JP2008541761A (enExample) |
| WO (1) | WO2006130632A2 (enExample) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9243241B2 (en) | 2005-05-31 | 2016-01-26 | Life Technologies Corporation | Separation and purification of nucleic acid from paraffin-containing samples |
| JP2008043332A (ja) * | 2006-08-17 | 2008-02-28 | Panomics Inc | 組織スライドからの核酸定量 |
| WO2009127350A1 (en) * | 2008-04-15 | 2009-10-22 | Universität Bern | Method for optimized isolation of rna from fixed tissue |
| DE102008029356A1 (de) * | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| DE202009018981U1 (de) | 2009-07-01 | 2015-03-11 | Axagarius Gmbh & Co. Kg | Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit dafür |
| DK2473596T3 (en) * | 2009-09-03 | 2018-01-22 | Becton Dickinson Co | METHODS AND COMPOSITIONS FOR DIRECT CHEMICAL LYSIS |
| CN102812118B (zh) | 2010-04-08 | 2016-01-20 | 恰根有限公司 | 分离和纯化核酸的方法 |
| JP2013528786A (ja) | 2010-04-08 | 2013-07-11 | キアゲン ゲーエムベーハー | 核酸を単離および精製するためのクロマトグラフィーデバイスおよび方法 |
| EP2395082A1 (en) * | 2010-06-14 | 2011-12-14 | QIAGEN GmbH | Extraction of nucleic acids from wax-embedded samples |
| US20120077198A1 (en) * | 2010-07-30 | 2012-03-29 | Ambergen, Inc | Compositions And Methods For Cancer Testing |
| TWI425977B (zh) | 2010-11-24 | 2014-02-11 | Ind Tech Res Inst | 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組 |
| JP6278568B2 (ja) * | 2011-12-06 | 2018-02-14 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 多相混合物からの生体分子を含む水相の回収 |
| EP2730653A1 (en) * | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
| TWI462930B (zh) | 2013-06-06 | 2014-12-01 | Rbc Bioscience Corp | 福馬林固定石蠟包埋樣本核酸分離方法 |
| US20160017314A1 (en) * | 2013-06-06 | 2016-01-21 | Rbc Bioscience Corp. | Method for isolating nucleic acids from formalin-fixed paraffin embedded tissue samples |
| JP6201587B2 (ja) * | 2013-09-30 | 2017-09-27 | 凸版印刷株式会社 | 包埋組織からの核酸抽出方法、包埋剤除去用具、及び核酸抽出用キット |
| CA2962782A1 (en) * | 2014-09-26 | 2016-03-31 | The Regents Of The University Of California | Methods and systems for detection of a genetic mutation |
| WO2016161023A1 (en) * | 2015-03-30 | 2016-10-06 | President And Fellows Of Harvard College | Methods to isolate high quality rna from fixed samples |
| US20190064037A1 (en) * | 2016-02-25 | 2019-02-28 | Massachusetts Institute Of Technology | Methods for Expanding Clinical Tissue Specimens |
| CN110050070B (zh) * | 2016-12-22 | 2023-12-22 | 文塔纳医疗系统公司 | 用于组织样品的全自动化核酸提取方法 |
| WO2018157074A1 (en) | 2017-02-24 | 2018-08-30 | Massachusetts Institute Of Technology | Methods for diagnosing neoplastic lesions |
| US11873374B2 (en) | 2018-02-06 | 2024-01-16 | Massachusetts Institute Of Technology | Swellable and structurally homogenous hydrogels and methods of use thereof |
| WO2020013833A1 (en) | 2018-07-13 | 2020-01-16 | Massachusetts Institute Of Technology | Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm) |
| CA3130889A1 (en) | 2019-02-22 | 2020-08-27 | Massachusetts Institute Of Technology | Iterative direct expansion microscopy |
| US12265004B2 (en) | 2019-11-05 | 2025-04-01 | Massachusetts Institute Of Technology | Membrane probes for expansion microscopy |
| EP4127164A1 (en) * | 2020-03-31 | 2023-02-08 | QIAGEN GmbH | Nucleic acid purification from fixed biological samples |
| CN113322303A (zh) * | 2021-05-12 | 2021-08-31 | 南京中科拜尔医学技术有限公司 | 一种石蜡切片样本核酸提取液及提取方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5092004A (en) * | 1988-10-11 | 1992-03-03 | Cosco, Inc, | Convertible infant restraint device |
| US5789156A (en) * | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
| CA2153215A1 (en) * | 1994-07-06 | 1996-01-07 | Lu Wang | Treatment of paraffin embedded tissue for gene analysis |
| US6090606A (en) * | 1996-01-24 | 2000-07-18 | Third Wave Technologies, Inc. | Cleavage agents |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US5912129A (en) * | 1998-03-05 | 1999-06-15 | Vinayagamoorthy; Thuraiayah | Multi-zone polymerase/ligase chain reaction |
| JPH11332562A (ja) | 1998-04-28 | 1999-12-07 | Ortho Clinical Diagnostics Inc | 組織試料およびパラフィン包埋組織から核酸を抽出するための改良方法 |
| US6183998B1 (en) * | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| US6544798B1 (en) * | 1999-02-26 | 2003-04-08 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| JP4679022B2 (ja) * | 1999-12-16 | 2011-04-27 | 学校法人片柳学園 | 標的塩基配列の検出方法 |
| DE60131485T2 (de) * | 2000-02-29 | 2008-10-09 | Kyocera Corp. | Leiterplatte |
| US7001724B1 (en) * | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| GB2369822A (en) | 2000-12-05 | 2002-06-12 | Genovar Diagnostics Ltd | Nucleic acid extraction method and kit |
| PL1601450T3 (pl) * | 2003-03-10 | 2014-03-31 | Expression Pathology Inc | Ciekły preparat tkankowy z próbek biologicznych, tkanek i komórek poddanych obróbce histopatologicznej |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| US9243241B2 (en) | 2005-05-31 | 2016-01-26 | Life Technologies Corporation | Separation and purification of nucleic acid from paraffin-containing samples |
-
2006
- 2006-05-31 US US11/444,171 patent/US9243241B2/en active Active
- 2006-05-31 JP JP2008514787A patent/JP2008541761A/ja active Pending
- 2006-05-31 WO PCT/US2006/021011 patent/WO2006130632A2/en not_active Ceased
- 2006-05-31 EP EP06771657A patent/EP1910389A4/en not_active Withdrawn
- 2006-10-23 US US11/552,063 patent/US20070128634A1/en not_active Abandoned
-
2016
- 2016-01-21 US US15/003,179 patent/US20160215281A1/en not_active Abandoned
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