JP2008541761A - パラフィン含有試料からの核酸の分離及び精製 - Google Patents

パラフィン含有試料からの核酸の分離及び精製 Download PDF

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JP2008541761A
JP2008541761A JP2008514787A JP2008514787A JP2008541761A JP 2008541761 A JP2008541761 A JP 2008541761A JP 2008514787 A JP2008514787 A JP 2008514787A JP 2008514787 A JP2008514787 A JP 2008514787A JP 2008541761 A JP2008541761 A JP 2008541761A
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minutes
paraffin
aqueous phase
warming
incubation
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JP2008541761A5 (enExample
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ソン‐ファ ケー
クレイグ ブランチ
ジュン リー
トッド ピーターソン
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インヴィトロジェン コーポレーション
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2008514787A 2005-05-31 2006-05-31 パラフィン含有試料からの核酸の分離及び精製 Pending JP2008541761A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US68652205P 2005-05-31 2005-05-31
US77302706P 2006-02-13 2006-02-13
PCT/US2006/021011 WO2006130632A2 (en) 2005-05-31 2006-05-31 Separation and purification of nucleic acid from paraffin-containing samples

Publications (2)

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JP2008541761A true JP2008541761A (ja) 2008-11-27
JP2008541761A5 JP2008541761A5 (enExample) 2010-09-24

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JP2008514787A Pending JP2008541761A (ja) 2005-05-31 2006-05-31 パラフィン含有試料からの核酸の分離及び精製

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US (3) US9243241B2 (enExample)
EP (1) EP1910389A4 (enExample)
JP (1) JP2008541761A (enExample)
WO (1) WO2006130632A2 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013530697A (ja) * 2010-06-14 2013-08-01 キアゲン ゲーエムベーハー ワックス包埋サンプルからの核酸抽出
WO2015046419A1 (ja) * 2013-09-30 2015-04-02 凸版印刷株式会社 包埋組織からの核酸抽出方法、包埋剤除去用具、及び核酸抽出用キット
JP2020503023A (ja) * 2016-12-22 2020-01-30 ヴェンタナ メディカル システムズ， インク． 組織試料用の全自動核酸抽出方法
JP2023521579A (ja) * 2020-03-31 2023-05-25 キアゲン ゲーエムベーハー 固定された生体試料からの核酸精製

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US9243241B2 (en) 2005-05-31 2016-01-26 Life Technologies Corporation Separation and purification of nucleic acid from paraffin-containing samples
JP2008043332A (ja) * 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
WO2009127350A1 (en) * 2008-04-15 2009-10-22 Universität Bern Method for optimized isolation of rna from fixed tissue
DE102008029356A1 (de) * 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
DE202009018981U1 (de) 2009-07-01 2015-03-11 Axagarius Gmbh & Co. Kg Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit dafür
DK2473596T3 (en) * 2009-09-03 2018-01-22 Becton Dickinson Co METHODS AND COMPOSITIONS FOR DIRECT CHEMICAL LYSIS
CN102812118B (zh) 2010-04-08 2016-01-20 恰根有限公司 分离和纯化核酸的方法
JP2013528786A (ja) 2010-04-08 2013-07-11 キアゲン ゲーエムベーハー 核酸を単離および精製するためのクロマトグラフィーデバイスおよび方法
US20120077198A1 (en) * 2010-07-30 2012-03-29 Ambergen, Inc Compositions And Methods For Cancer Testing
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
JP6278568B2 (ja) * 2011-12-06 2018-02-14 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 多相混合物からの生体分子を含む水相の回収
EP2730653A1 (en) * 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
TWI462930B (zh) 2013-06-06 2014-12-01 Rbc Bioscience Corp 福馬林固定石蠟包埋樣本核酸分離方法
US20160017314A1 (en) * 2013-06-06 2016-01-21 Rbc Bioscience Corp. Method for isolating nucleic acids from formalin-fixed paraffin embedded tissue samples
CA2962782A1 (en) * 2014-09-26 2016-03-31 The Regents Of The University Of California Methods and systems for detection of a genetic mutation
WO2016161023A1 (en) * 2015-03-30 2016-10-06 President And Fellows Of Harvard College Methods to isolate high quality rna from fixed samples
US20190064037A1 (en) * 2016-02-25 2019-02-28 Massachusetts Institute Of Technology Methods for Expanding Clinical Tissue Specimens
WO2018157074A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for diagnosing neoplastic lesions
US11873374B2 (en) 2018-02-06 2024-01-16 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
WO2020013833A1 (en) 2018-07-13 2020-01-16 Massachusetts Institute Of Technology Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm)
CA3130889A1 (en) 2019-02-22 2020-08-27 Massachusetts Institute Of Technology Iterative direct expansion microscopy
US12265004B2 (en) 2019-11-05 2025-04-01 Massachusetts Institute Of Technology Membrane probes for expansion microscopy
CN113322303A (zh) * 2021-05-12 2021-08-31 南京中科拜尔医学技术有限公司 一种石蜡切片样本核酸提取液及提取方法

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JPH11332562A (ja) * 1998-04-28 1999-12-07 Ortho Clinical Diagnostics Inc 組織試料およびパラフィン包埋組織から核酸を抽出するための改良方法
JP2004514886A (ja) * 2000-11-22 2004-05-20 ベンタナ・メデイカル・システムズ・インコーポレーテツド 自動染色機器における生物サンプルからの包埋媒質の除去および細胞コンディショニング

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CA2153215A1 (en) * 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
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US5912129A (en) * 1998-03-05 1999-06-15 Vinayagamoorthy; Thuraiayah Multi-zone polymerase/ligase chain reaction
US6183998B1 (en) * 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6469159B1 (en) * 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
JP4679022B2 (ja) * 1999-12-16 2011-04-27 学校法人片柳学園 標的塩基配列の検出方法
DE60131485T2 (de) * 2000-02-29 2008-10-09 Kyocera Corp. Leiterplatte
US7001724B1 (en) * 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
GB2369822A (en) 2000-12-05 2002-06-12 Genovar Diagnostics Ltd Nucleic acid extraction method and kit
PL1601450T3 (pl) * 2003-03-10 2014-03-31 Expression Pathology Inc Ciekły preparat tkankowy z próbek biologicznych, tkanek i komórek poddanych obróbce histopatologicznej
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
US9243241B2 (en) 2005-05-31 2016-01-26 Life Technologies Corporation Separation and purification of nucleic acid from paraffin-containing samples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11332562A (ja) * 1998-04-28 1999-12-07 Ortho Clinical Diagnostics Inc 組織試料およびパラフィン包埋組織から核酸を抽出するための改良方法
JP2004514886A (ja) * 2000-11-22 2004-05-20 ベンタナ・メデイカル・システムズ・インコーポレーテツド 自動染色機器における生物サンプルからの包埋媒質の除去および細胞コンディショニング

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013530697A (ja) * 2010-06-14 2013-08-01 キアゲン ゲーエムベーハー ワックス包埋サンプルからの核酸抽出
WO2015046419A1 (ja) * 2013-09-30 2015-04-02 凸版印刷株式会社 包埋組織からの核酸抽出方法、包埋剤除去用具、及び核酸抽出用キット
JP2015065933A (ja) * 2013-09-30 2015-04-13 凸版印刷株式会社 包埋組織からの核酸抽出方法、包埋剤除去用具、及び核酸抽出用キット
JP2020503023A (ja) * 2016-12-22 2020-01-30 ヴェンタナ メディカル システムズ， インク． 組織試料用の全自動核酸抽出方法
JP2023521579A (ja) * 2020-03-31 2023-05-25 キアゲン ゲーエムベーハー 固定された生体試料からの核酸精製

Also Published As

Publication number Publication date
US20070026432A1 (en) 2007-02-01
EP1910389A2 (en) 2008-04-16
EP1910389A4 (en) 2010-03-10
US9243241B2 (en) 2016-01-26
US20160215281A1 (en) 2016-07-28
WO2006130632A3 (en) 2007-03-22
WO2006130632A2 (en) 2006-12-07
US20070128634A1 (en) 2007-06-07

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