JP2008527987A5 - - Google Patents
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- Publication number
- JP2008527987A5 JP2008527987A5 JP2007551737A JP2007551737A JP2008527987A5 JP 2008527987 A5 JP2008527987 A5 JP 2008527987A5 JP 2007551737 A JP2007551737 A JP 2007551737A JP 2007551737 A JP2007551737 A JP 2007551737A JP 2008527987 A5 JP2008527987 A5 JP 2008527987A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- transposon
- sequence
- restriction enzyme
- dna sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 25
- 238000000034 method Methods 0.000 claims 24
- 108020004414 DNA Proteins 0.000 claims 21
- 108091008146 restriction endonucleases Proteins 0.000 claims 21
- 108090000623 proteins and genes Proteins 0.000 claims 10
- 229920001184 polypeptide Polymers 0.000 claims 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims 9
- 125000003275 alpha amino acid group Chemical group 0.000 claims 7
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 102000008579 Transposases Human genes 0.000 claims 4
- 108010020764 Transposases Proteins 0.000 claims 4
- 150000001413 amino acids Chemical class 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 239000013612 plasmid Substances 0.000 claims 3
- 238000006276 transfer reaction Methods 0.000 claims 3
- 230000007018 DNA scission Effects 0.000 claims 2
- 238000003776 cleavage reaction Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 230000007017 scission Effects 0.000 claims 2
- 238000006467 substitution reaction Methods 0.000 claims 2
- 108020004705 Codon Proteins 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- 108700026244 Open Reading Frames Proteins 0.000 claims 1
- 108091081024 Start codon Proteins 0.000 claims 1
- 230000003281 allosteric effect Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 238000006384 oligomerization reaction Methods 0.000 claims 1
- 108020001580 protein domains Proteins 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 230000017105 transposition Effects 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0501189.5A GB0501189D0 (en) | 2005-01-20 | 2005-01-20 | Polypeptide mutagenesis method |
| PCT/GB2006/000187 WO2006077411A1 (en) | 2005-01-20 | 2006-01-19 | Polypeptide mutagenesis method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008527987A JP2008527987A (ja) | 2008-07-31 |
| JP2008527987A5 true JP2008527987A5 (enExample) | 2009-02-26 |
Family
ID=34259403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007551737A Pending JP2008527987A (ja) | 2005-01-20 | 2006-01-19 | ポリペプチド突然変異導入法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20090053761A1 (enExample) |
| EP (1) | EP1838851B1 (enExample) |
| JP (1) | JP2008527987A (enExample) |
| AT (1) | ATE443137T1 (enExample) |
| AU (1) | AU2006207308A1 (enExample) |
| CA (1) | CA2601324A1 (enExample) |
| DE (1) | DE602006009217D1 (enExample) |
| DK (1) | DK1838851T3 (enExample) |
| GB (1) | GB0501189D0 (enExample) |
| WO (1) | WO2006077411A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0515131D0 (en) * | 2005-07-22 | 2005-08-31 | Univ Aston | Oligonucleotide library encoding randomised peptides |
| US20150337290A1 (en) * | 2012-09-07 | 2015-11-26 | Alexander Gubin | Methods and products for mutating nucleotide sequences |
| LT3027771T (lt) * | 2013-07-30 | 2019-04-25 | Gen9, Inc. | Ilgos kloninės sekos verifikuotų nukleorūgščių konstruktų gamybos būdas |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4830965A (en) * | 1983-12-12 | 1989-05-16 | Canadian Patents And Development Ltd. | Transposable linkers to transfer genetic information |
| US5677170A (en) * | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| DE10109517A1 (de) * | 2001-02-28 | 2002-08-29 | Volker Sieber | Verfahren zur Herstellung von Genbibliotheken |
| US20020119573A1 (en) | 2001-02-28 | 2002-08-29 | Shaw Karen J. | Footprinting plasmid |
| JP2002253255A (ja) * | 2001-03-01 | 2002-09-10 | Japan Science & Technology Corp | ランダム挿入削除ｄｎａ変異法 |
| FI20020746A7 (fi) * | 2002-04-18 | 2003-10-19 | Finnzymes Oy | Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi |
| KR100391884B1 (en) * | 2002-07-04 | 2003-09-06 | Amicogen Co Ltd | Random codon based mutagenesis using transposon |
-
2005
- 2005-01-20 GB GBGB0501189.5A patent/GB0501189D0/en not_active Ceased
-
2006
- 2006-01-19 EP EP06703246A patent/EP1838851B1/en not_active Not-in-force
- 2006-01-19 JP JP2007551737A patent/JP2008527987A/ja active Pending
- 2006-01-19 US US11/795,732 patent/US20090053761A1/en not_active Abandoned
- 2006-01-19 AT AT06703246T patent/ATE443137T1/de not_active IP Right Cessation
- 2006-01-19 AU AU2006207308A patent/AU2006207308A1/en not_active Abandoned
- 2006-01-19 CA CA002601324A patent/CA2601324A1/en not_active Abandoned
- 2006-01-19 WO PCT/GB2006/000187 patent/WO2006077411A1/en not_active Ceased
- 2006-01-19 DE DE602006009217T patent/DE602006009217D1/de active Active
- 2006-01-19 DK DK06703246.6T patent/DK1838851T3/da active
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