DK1838851T3 - Fremgangsmåde til polypeptidmutagenese - Google Patents

Fremgangsmåde til polypeptidmutagenese

Info

Publication number
DK1838851T3
DK1838851T3 DK06703246.6T DK06703246T DK1838851T3 DK 1838851 T3 DK1838851 T3 DK 1838851T3 DK 06703246 T DK06703246 T DK 06703246T DK 1838851 T3 DK1838851 T3 DK 1838851T3
Authority
DK
Denmark
Prior art keywords
transposon
restriction enzyme
sequence
target dna
polypeptide
Prior art date
Application number
DK06703246.6T
Other languages
English (en)
Inventor
Dafydd Jones
Original Assignee
Univ Cardiff
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34259403&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1838851(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Cardiff filed Critical Univ Cardiff
Application granted granted Critical
Publication of DK1838851T3 publication Critical patent/DK1838851T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Wrappers (AREA)
  • Polyoxymethylene Polymers And Polymers With Carbon-To-Carbon Bonds (AREA)
  • Laminated Bodies (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK06703246.6T 2005-01-20 2006-01-19 Fremgangsmåde til polypeptidmutagenese DK1838851T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0501189.5A GB0501189D0 (en) 2005-01-20 2005-01-20 Polypeptide mutagenesis method
PCT/GB2006/000187 WO2006077411A1 (en) 2005-01-20 2006-01-19 Polypeptide mutagenesis method

Publications (1)

Publication Number Publication Date
DK1838851T3 true DK1838851T3 (da) 2010-01-25

Family

ID=34259403

Family Applications (1)

Application Number Title Priority Date Filing Date
DK06703246.6T DK1838851T3 (da) 2005-01-20 2006-01-19 Fremgangsmåde til polypeptidmutagenese

Country Status (10)

Country Link
US (1) US20090053761A1 (da)
EP (1) EP1838851B1 (da)
JP (1) JP2008527987A (da)
AT (1) ATE443137T1 (da)
AU (1) AU2006207308A1 (da)
CA (1) CA2601324A1 (da)
DE (1) DE602006009217D1 (da)
DK (1) DK1838851T3 (da)
GB (1) GB0501189D0 (da)
WO (1) WO2006077411A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0515131D0 (en) * 2005-07-22 2005-08-31 Univ Aston Oligonucleotide library encoding randomised peptides
WO2014039662A1 (en) * 2012-09-07 2014-03-13 Alexander Gubin Methods and products for mutating nucleotide sequences
US20160168564A1 (en) * 2013-07-30 2016-06-16 Gen9, Inc. Methods for the Production of Long Length Clonal Sequence Verified Nucleic Acid Constructs

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830965A (en) * 1983-12-12 1989-05-16 Canadian Patents And Development Ltd. Transposable linkers to transfer genetic information
US5677170A (en) * 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US20020119573A1 (en) 2001-02-28 2002-08-29 Shaw Karen J. Footprinting plasmid
DE10109517A1 (de) * 2001-02-28 2002-08-29 Volker Sieber Verfahren zur Herstellung von Genbibliotheken
JP2002253255A (ja) * 2001-03-01 2002-09-10 Japan Science & Technology Corp ランダム挿入削除ｄｎａ変異法
FI20020746A (fi) * 2002-04-18 2003-10-19 Finnzymes Oy Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi
KR100391884B1 (en) * 2002-07-04 2003-09-06 Amicogen Co Ltd Random codon based mutagenesis using transposon

Also Published As

Publication number Publication date
GB0501189D0 (en) 2005-03-02
EP1838851B1 (en) 2009-09-16
JP2008527987A (ja) 2008-07-31
AU2006207308A1 (en) 2006-07-27
WO2006077411A1 (en) 2006-07-27
US20090053761A1 (en) 2009-02-26
ATE443137T1 (de) 2009-10-15
DE602006009217D1 (en) 2009-10-29
CA2601324A1 (en) 2006-07-27
EP1838851A1 (en) 2007-10-03

Similar Documents

Publication Publication Date Title
HK1210627A1 (en) Preparing crude biological estracts using protease, suitable for preparing cdna cdna
BRPI0511868A (pt) peptìdeo isolado, polipeptìdeo de fusão, moléculas de ácido nucléico isoladas, vetores, métodos de direcionamento de polipeptìdeos e método de identificação de peptìdeos
AR062069A1 (es) Replegado de proteinas recombinantes
WO2007139898A3 (en) Variant foki cleavage half-domains
WO2006059113A3 (en) Fusion proteins comprising a non-cytotoxic protease, a targeting moiety, a protease cleavage site and a translocation domain
HK1125387A1 (en) Compositions and methods for fusion protein separation
MX2010002753A (es) Plantas que tienen rasgos aumentados relacionados con el rendimiento y un metodo para obtenerlas.
EP1994142A4 (en) METHODS AND COMPOSITIONS FOR RAPID ISOLATION OF SMALL RNA MOLECULES
ATE497492T1 (de) Reinigung von montelukast
BR122018016045B8 (pt) proteína variante otimizada
DK1920248T3 (da) Clostridialt-toksin-aktivitetsassays
ES2688623T3 (es) Proteínas de fusión que se unen a factores de crecimiento
DK1859125T3 (da) Anvendelse af vandoplöselige alkansulfonsyrer til forögelse af permeabiliteten af underjordiske carbonatiske råolie- og/eller naturgasledende stenformationer og til oplösning af carbonatiske og/eller carbonatholdige forureninger ved råolieproduktionen
ATE516368T1 (de) Erstellung eines echtzeit-genexpressionsprofils
DK1863920T3 (da) Fremgangsmåde til forbedret isolering af rekombinant producerede proteiner
MX2009012451A (es) Plantas con rasgos mejorados relacionados con el rendimiento y un metodo para producirlas.
EP2599870A3 (en) Plants having enhanced yield-related traits and a method for making the same
EA201290168A1 (ru) Растения, обладающие повышенными урожайностными свойствами, и способ создания таких растений
WO2002103046A3 (en) Happier mapping
IN2009CN03372A (da)
EA200702193A1 (ru) Гликозилирование белков
GB2489187A (en) Compositions,methods and related uses for cleaving modified DNA
WO2009066320A3 (en) Gcsf fusion protein systems suitable for high expression of peptides
WO2008033555A3 (en) High-pressure refolding of difficult-to-fold proteins
NO20055210L (no) Inhibitorproteiner av en protease og anvendelse derav