DE602006009217D1 - Polypeptidmutageneseverfahren - Google Patents

Polypeptidmutageneseverfahren

Info

Publication number
DE602006009217D1
DE602006009217D1 DE602006009217T DE602006009217T DE602006009217D1 DE 602006009217 D1 DE602006009217 D1 DE 602006009217D1 DE 602006009217 T DE602006009217 T DE 602006009217T DE 602006009217 T DE602006009217 T DE 602006009217T DE 602006009217 D1 DE602006009217 D1 DE 602006009217D1
Authority
DE
Germany
Prior art keywords
transposon
restriction enzyme
sequence
target dna
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006009217T
Other languages
English (en)
Inventor
Dafydd Jones
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College Cardiff Consultants Ltd
Cardiff University
Original Assignee
University College Cardiff Consultants Ltd
Cardiff University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34259403&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602006009217(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University College Cardiff Consultants Ltd, Cardiff University filed Critical University College Cardiff Consultants Ltd
Publication of DE602006009217D1 publication Critical patent/DE602006009217D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Laminated Bodies (AREA)
  • Wrappers (AREA)
  • Polyoxymethylene Polymers And Polymers With Carbon-To-Carbon Bonds (AREA)
DE602006009217T 2005-01-20 2006-01-19 Polypeptidmutageneseverfahren Active DE602006009217D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0501189.5A GB0501189D0 (en) 2005-01-20 2005-01-20 Polypeptide mutagenesis method
PCT/GB2006/000187 WO2006077411A1 (en) 2005-01-20 2006-01-19 Polypeptide mutagenesis method

Publications (1)

Publication Number Publication Date
DE602006009217D1 true DE602006009217D1 (en) 2009-10-29

Family

ID=34259403

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006009217T Active DE602006009217D1 (en) 2005-01-20 2006-01-19 Polypeptidmutageneseverfahren

Country Status (10)

Country Link
US (1) US20090053761A1 (de)
EP (1) EP1838851B1 (de)
JP (1) JP2008527987A (de)
AT (1) ATE443137T1 (de)
AU (1) AU2006207308A1 (de)
CA (1) CA2601324A1 (de)
DE (1) DE602006009217D1 (de)
DK (1) DK1838851T3 (de)
GB (1) GB0501189D0 (de)
WO (1) WO2006077411A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0515131D0 (en) * 2005-07-22 2005-08-31 Univ Aston Oligonucleotide library encoding randomised peptides
WO2014039662A1 (en) * 2012-09-07 2014-03-13 Alexander Gubin Methods and products for mutating nucleotide sequences
US20160168564A1 (en) * 2013-07-30 2016-06-16 Gen9, Inc. Methods for the Production of Long Length Clonal Sequence Verified Nucleic Acid Constructs

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830965A (en) * 1983-12-12 1989-05-16 Canadian Patents And Development Ltd. Transposable linkers to transfer genetic information
US5677170A (en) * 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US20020119573A1 (en) 2001-02-28 2002-08-29 Shaw Karen J. Footprinting plasmid
DE10109517A1 (de) * 2001-02-28 2002-08-29 Volker Sieber Verfahren zur Herstellung von Genbibliotheken
JP2002253255A (ja) * 2001-03-01 2002-09-10 Japan Science & Technology Corp ランダム挿入削除ｄｎａ変異法
FI20020746A (fi) * 2002-04-18 2003-10-19 Finnzymes Oy Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi
KR100391884B1 (en) * 2002-07-04 2003-09-06 Amicogen Co Ltd Random codon based mutagenesis using transposon

Also Published As

Publication number Publication date
AU2006207308A1 (en) 2006-07-27
ATE443137T1 (de) 2009-10-15
CA2601324A1 (en) 2006-07-27
DK1838851T3 (da) 2010-01-25
EP1838851A1 (de) 2007-10-03
US20090053761A1 (en) 2009-02-26
GB0501189D0 (en) 2005-03-02
JP2008527987A (ja) 2008-07-31
EP1838851B1 (de) 2009-09-16
WO2006077411A1 (en) 2006-07-27

Similar Documents

Publication Publication Date Title
WO2007139898A3 (en) Variant foki cleavage half-domains
WO2006059113A3 (en) Fusion proteins comprising a non-cytotoxic protease, a targeting moiety, a protease cleavage site and a translocation domain
WO2007130453A3 (en) Non-natural amino acid substituted polypeptides
BRPI0511868A (pt) peptìdeo isolado, polipeptìdeo de fusão, moléculas de ácido nucléico isoladas, vetores, métodos de direcionamento de polipeptìdeos e método de identificação de peptìdeos
HK1125387A1 (en) Compositions and methods for fusion protein separation
MX2010004325A (es) Secuencias de señal y chaperones co-expresados para mejorar produccion de proteinas en una celula hospedera.
ES2688623T3 (es) Proteínas de fusión que se unen a factores de crecimiento
WO2007098201A3 (en) Shiga toxoid chimeric proteins
UA94019C2 (ru) Антитело, которое специфически связывается с глипиканом 3 (gpc3)
WO2005047511A3 (en) Bacterial expression of protease inhibitors and variants thereof
DE602006017237D1 (en) Clostridientoxinaktivitätstests
WO2010051049A3 (en) Thermophilic and thermoacidophilic glycosylation genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
WO2008092935A3 (en) Plants having enhanced yield-related traits and/or increased abiotic stress resistance, and a method for making the same
MX2009012451A (es) Plantas con rasgos mejorados relacionados con el rendimiento y un metodo para producirlas.
WO2009124255A8 (en) Methods for transcript analysis
WO2002103046A3 (en) Happier mapping
CL2009001601A1 (es) Secuencia reguladora aisladas capaces de iniciar y conducir la transcripcion de un polinucleotido heterólogo en plantas, construccion génica que comprende dichos acidos nucleicos y metodo para producir una planta con un fenotipo modificado. (divisional solicitud n° 1425-05)
GB2489187A (en) Compositions,methods and related uses for cleaving modified DNA
WO2009066320A3 (en) Gcsf fusion protein systems suitable for high expression of peptides
WO2008033555A3 (en) High-pressure refolding of difficult-to-fold proteins
WO2008033556A3 (en) High pressure treatment of proteins for reduced immunogenicity
WO2004056986A3 (en) Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
DE602006009217D1 (en) Polypeptidmutageneseverfahren
WO2007083100A3 (en) Enzyme
EA201071408A1 (ru) Способ получения однодольных растений с мужской стерильностью

Legal Events

Date Code Title Description
8363 Opposition against the patent