CA2601324A1 - Polypeptide mutagenesis method - Google Patents
Polypeptide mutagenesis method Download PDFInfo
- Publication number
- CA2601324A1 CA2601324A1 CA002601324A CA2601324A CA2601324A1 CA 2601324 A1 CA2601324 A1 CA 2601324A1 CA 002601324 A CA002601324 A CA 002601324A CA 2601324 A CA2601324 A CA 2601324A CA 2601324 A1 CA2601324 A1 CA 2601324A1
- Authority
- CA
- Canada
- Prior art keywords
- transposon
- dna
- sequence
- amino acid
- restriction enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Laminated Bodies (AREA)
- Wrappers (AREA)
- Polyoxymethylene Polymers And Polymers With Carbon-To-Carbon Bonds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0501189.5A GB0501189D0 (en) | 2005-01-20 | 2005-01-20 | Polypeptide mutagenesis method |
| GB0501189.5 | 2005-01-20 | ||
| PCT/GB2006/000187 WO2006077411A1 (en) | 2005-01-20 | 2006-01-19 | Polypeptide mutagenesis method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2601324A1 true CA2601324A1 (en) | 2006-07-27 |
Family
ID=34259403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002601324A Abandoned CA2601324A1 (en) | 2005-01-20 | 2006-01-19 | Polypeptide mutagenesis method |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20090053761A1 (enExample) |
| EP (1) | EP1838851B1 (enExample) |
| JP (1) | JP2008527987A (enExample) |
| AT (1) | ATE443137T1 (enExample) |
| AU (1) | AU2006207308A1 (enExample) |
| CA (1) | CA2601324A1 (enExample) |
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| DK (1) | DK1838851T3 (enExample) |
| GB (1) | GB0501189D0 (enExample) |
| WO (1) | WO2006077411A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0515131D0 (en) * | 2005-07-22 | 2005-08-31 | Univ Aston | Oligonucleotide library encoding randomised peptides |
| US20150337290A1 (en) * | 2012-09-07 | 2015-11-26 | Alexander Gubin | Methods and products for mutating nucleotide sequences |
| LT3027771T (lt) * | 2013-07-30 | 2019-04-25 | Gen9, Inc. | Ilgos kloninės sekos verifikuotų nukleorūgščių konstruktų gamybos būdas |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4830965A (en) * | 1983-12-12 | 1989-05-16 | Canadian Patents And Development Ltd. | Transposable linkers to transfer genetic information |
| US5677170A (en) * | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| DE10109517A1 (de) * | 2001-02-28 | 2002-08-29 | Volker Sieber | Verfahren zur Herstellung von Genbibliotheken |
| US20020119573A1 (en) | 2001-02-28 | 2002-08-29 | Shaw Karen J. | Footprinting plasmid |
| JP2002253255A (ja) * | 2001-03-01 | 2002-09-10 | Japan Science & Technology Corp | ランダム挿入削除ｄｎａ変異法 |
| FI20020746A7 (fi) * | 2002-04-18 | 2003-10-19 | Finnzymes Oy | Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi |
| KR100391884B1 (en) * | 2002-07-04 | 2003-09-06 | Amicogen Co Ltd | Random codon based mutagenesis using transposon |
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2005
- 2005-01-20 GB GBGB0501189.5A patent/GB0501189D0/en not_active Ceased
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2006
- 2006-01-19 EP EP06703246A patent/EP1838851B1/en not_active Not-in-force
- 2006-01-19 JP JP2007551737A patent/JP2008527987A/ja active Pending
- 2006-01-19 US US11/795,732 patent/US20090053761A1/en not_active Abandoned
- 2006-01-19 AT AT06703246T patent/ATE443137T1/de not_active IP Right Cessation
- 2006-01-19 AU AU2006207308A patent/AU2006207308A1/en not_active Abandoned
- 2006-01-19 CA CA002601324A patent/CA2601324A1/en not_active Abandoned
- 2006-01-19 WO PCT/GB2006/000187 patent/WO2006077411A1/en not_active Ceased
- 2006-01-19 DE DE602006009217T patent/DE602006009217D1/de active Active
- 2006-01-19 DK DK06703246.6T patent/DK1838851T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1838851A1 (en) | 2007-10-03 |
| US20090053761A1 (en) | 2009-02-26 |
| ATE443137T1 (de) | 2009-10-15 |
| DK1838851T3 (da) | 2010-01-25 |
| JP2008527987A (ja) | 2008-07-31 |
| EP1838851B1 (en) | 2009-09-16 |
| DE602006009217D1 (en) | 2009-10-29 |
| AU2006207308A1 (en) | 2006-07-27 |
| WO2006077411A1 (en) | 2006-07-27 |
| GB0501189D0 (en) | 2005-03-02 |
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| Date | Code | Title | Description |
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| EEER | Examination request | ||
| FZDE | Discontinued |
Effective date: 20130121 |