JP2005508194A5 - - Google Patents
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- Publication number
- JP2005508194A5 JP2005508194A5 JP2003542637A JP2003542637A JP2005508194A5 JP 2005508194 A5 JP2005508194 A5 JP 2005508194A5 JP 2003542637 A JP2003542637 A JP 2003542637A JP 2003542637 A JP2003542637 A JP 2003542637A JP 2005508194 A5 JP2005508194 A5 JP 2005508194A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- library
- acid sequence
- protein
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 43
- 150000007523 nucleic acids Chemical group 0.000 claims 32
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 21
- 239000012634 fragment Substances 0.000 claims 21
- 239000002773 nucleotide Substances 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 20
- 102000004169 proteins and genes Human genes 0.000 claims 18
- 108090000623 proteins and genes Proteins 0.000 claims 18
- 108090000765 processed proteins & peptides Proteins 0.000 claims 17
- 108020001580 protein domains Proteins 0.000 claims 15
- 229920001184 polypeptide Polymers 0.000 claims 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims 12
- 230000027455 binding Effects 0.000 claims 11
- 102000004190 Enzymes Human genes 0.000 claims 5
- 108090000790 Enzymes Proteins 0.000 claims 5
- 239000013604 expression vector Substances 0.000 claims 5
- 102000000584 Calmodulin Human genes 0.000 claims 3
- 108010041952 Calmodulin Proteins 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 239000000376 reactant Substances 0.000 claims 3
- FSASIHFSFGAIJM-UHFFFAOYSA-N 3-methyladenine Chemical compound CN1C=NC(N)=C2N=CN=C12 FSASIHFSFGAIJM-UHFFFAOYSA-N 0.000 claims 2
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 108010042407 Endonucleases Proteins 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical group O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 2
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 2
- 125000000539 amino acid group Chemical group 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 229920002704 polyhistidine Polymers 0.000 claims 2
- 108010066533 ribonuclease S Proteins 0.000 claims 2
- 239000007790 solid phase Substances 0.000 claims 2
- JTBBWRKSUYCPFY-UHFFFAOYSA-N 2,3-dihydro-1h-pyrimidin-4-one Chemical group O=C1NCNC=C1 JTBBWRKSUYCPFY-UHFFFAOYSA-N 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 108020001738 DNA Glycosylase Proteins 0.000 claims 1
- 102000028381 DNA glycosylase Human genes 0.000 claims 1
- 238000002965 ELISA Methods 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 230000004071 biological effect Effects 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 claims 1
- 239000002198 insoluble material Substances 0.000 claims 1
- 239000003446 ligand Substances 0.000 claims 1
- 238000001972 liquid chromatography-electrospray ionisation mass spectrometry Methods 0.000 claims 1
- 239000006166 lysate Substances 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 150000003384 small molecules Chemical class 0.000 claims 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 239000004094 surface-active agent Substances 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0126887.9A GB0126887D0 (en) | 2001-11-08 | 2001-11-08 | Method for producing and identifying soluble protein domains |
| PCT/GB2002/005075 WO2003040391A2 (en) | 2001-11-08 | 2002-11-08 | Method for producing and identifying soluble protein domains |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005508194A JP2005508194A (ja) | 2005-03-31 |
| JP2005508194A5 true JP2005508194A5 (enExample) | 2005-12-22 |
| JP4303112B2 JP4303112B2 (ja) | 2009-07-29 |
Family
ID=9925436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003542637A Expired - Fee Related JP4303112B2 (ja) | 2001-11-08 | 2002-11-08 | 可溶性蛋白質ドメインの生成及び同定のための方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8852866B2 (enExample) |
| EP (1) | EP1442134B1 (enExample) |
| JP (1) | JP4303112B2 (enExample) |
| AT (1) | ATE408029T1 (enExample) |
| AU (1) | AU2002341204B2 (enExample) |
| CA (1) | CA2465377C (enExample) |
| DE (1) | DE60228867D1 (enExample) |
| DK (1) | DK1442134T3 (enExample) |
| GB (1) | GB0126887D0 (enExample) |
| WO (1) | WO2003040391A2 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE452992T1 (de) | 2004-02-04 | 2010-01-15 | Qiagen North American Holdings | Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation |
| EP1907571B1 (en) | 2005-06-15 | 2017-04-26 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| US7790364B2 (en) * | 2006-01-27 | 2010-09-07 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods for high throughput screening of pharmacological chaperones |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| CN107267596A (zh) * | 2009-06-15 | 2017-10-20 | 考利达基因组股份有限公司 | 用于长片段阅读测序的方法和组合物 |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| JP2011147403A (ja) * | 2010-01-22 | 2011-08-04 | Hitachi High-Technologies Corp | 細菌検査装置及び細菌検査方法 |
| KR101629773B1 (ko) * | 2014-07-16 | 2016-06-13 | 한국과학기술원 | 약물 펩타이드 후보 선별 장치 및 이를 이용한 약물 펩타이드 후보 선별 방법 |
| DK3294934T3 (da) * | 2015-07-03 | 2020-03-23 | Hunan Zonsen Peplib Biotech Co Ltd | Fremgangsmåde til opbygning af peptidbibliotek og relaterede vektorer |
| EP3596106B1 (en) * | 2017-04-26 | 2021-08-11 | Hunan Zonsen Peplib Biotech Co., Ltd | Peptide library constructing method |
| IL312717A (en) * | 2021-11-17 | 2024-07-01 | Nerd Bio Llc | Systems and methods for real-time cellular engagement with DRUG-TARGET |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2067194C (en) * | 1989-10-05 | 2003-03-18 | Glenn Kawasaki | Cell-free synthesis and isolation of novel genes and polypeptides |
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5805923A (en) * | 1995-05-26 | 1998-09-08 | Sony Corporation | Configurable power management system having a clock stabilization filter that can be enabled or bypassed depending upon whether a crystal or can oscillator is used |
| US6190865B1 (en) | 1995-09-27 | 2001-02-20 | Epicentre Technologies Corporation | Method for characterizing nucleic acid molecules |
| DE19545320A1 (de) * | 1995-12-05 | 1997-06-12 | Boehringer Mannheim Gmbh | Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA |
| ATE214104T1 (de) | 1998-04-22 | 2002-03-15 | Entpr Ie Trd As Bioresearch Ie | Verfahren zur charakterisierung von nukleinsäuremolekulen, das die erzeugung von verlängerbaren aufwärtsgelegenen dns-fragmenten, die durch spaltung der nukleinsäure an einer abasischen stelle entsteht, umfasst |
| EP2230303B1 (en) * | 1999-05-05 | 2013-01-16 | Phylogica Limited | Isolating biological modulators from biodiverse gene fragment libraries |
| EP1276900A2 (en) * | 2000-01-11 | 2003-01-22 | Maxygen, Inc. | Integrated systems and methods for diversity generation and screening |
| WO2001070947A2 (en) * | 2000-03-20 | 2001-09-27 | Maxygen, Inc. | Method for generating recombinant dna molecules in complex mixtures |
| WO2004072239A2 (en) * | 2003-02-06 | 2004-08-26 | Verdia, Inc. | Novel polypeptides with antifungal activity |
-
2001
- 2001-11-08 GB GBGB0126887.9A patent/GB0126887D0/en not_active Ceased
-
2002
- 2002-11-08 DK DK02774994T patent/DK1442134T3/da active
- 2002-11-08 WO PCT/GB2002/005075 patent/WO2003040391A2/en not_active Ceased
- 2002-11-08 JP JP2003542637A patent/JP4303112B2/ja not_active Expired - Fee Related
- 2002-11-08 DE DE60228867T patent/DE60228867D1/de not_active Expired - Lifetime
- 2002-11-08 AU AU2002341204A patent/AU2002341204B2/en not_active Ceased
- 2002-11-08 US US10/494,895 patent/US8852866B2/en not_active Expired - Fee Related
- 2002-11-08 EP EP02774994A patent/EP1442134B1/en not_active Expired - Lifetime
- 2002-11-08 AT AT02774994T patent/ATE408029T1/de not_active IP Right Cessation
- 2002-11-08 CA CA2465377A patent/CA2465377C/en not_active Expired - Lifetime
-
2014
- 2014-09-05 US US14/478,773 patent/US20150065382A1/en not_active Abandoned
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