AU2002341204B2 - Method for producing and identifying soluble protein domains - Google Patents

Method for producing and identifying soluble protein domains Download PDF

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Publication number
AU2002341204B2
AU2002341204B2 AU2002341204A AU2002341204A AU2002341204B2 AU 2002341204 B2 AU2002341204 B2 AU 2002341204B2 AU 2002341204 A AU2002341204 A AU 2002341204A AU 2002341204 A AU2002341204 A AU 2002341204A AU 2002341204 B2 AU2002341204 B2 AU 2002341204B2
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AU
Australia
Prior art keywords
fragments
nucleic acid
protein
library
dna
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2002341204A
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English (en)
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AU2002341204A1 (en
Inventor
Paul Driscoll
Mark Mcalister
Laurence Pearl
Chrisostomos Prodromou
Renos Savva
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Domainex Ltd
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Domainex Ltd
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Assigned to DOMAINEX LIMITED reassignment DOMAINEX LIMITED Request for Assignment Assignors: BIRKBECK COLLEGE, THE INSTITUTE OF CANCER RESEARCH, UNIVERSITY COLLEGE LONDON
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2002341204A 2001-11-08 2002-11-08 Method for producing and identifying soluble protein domains Ceased AU2002341204B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0126887.9A GB0126887D0 (en) 2001-11-08 2001-11-08 Method for producing and identifying soluble protein domains
GB0126887.9 2001-11-08
PCT/GB2002/005075 WO2003040391A2 (en) 2001-11-08 2002-11-08 Method for producing and identifying soluble protein domains

Publications (2)

Publication Number Publication Date
AU2002341204A1 AU2002341204A1 (en) 2003-07-24
AU2002341204B2 true AU2002341204B2 (en) 2006-10-19

Family

ID=9925436

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002341204A Ceased AU2002341204B2 (en) 2001-11-08 2002-11-08 Method for producing and identifying soluble protein domains

Country Status (10)

Country Link
US (2) US8852866B2 (enExample)
EP (1) EP1442134B1 (enExample)
JP (1) JP4303112B2 (enExample)
AT (1) ATE408029T1 (enExample)
AU (1) AU2002341204B2 (enExample)
CA (1) CA2465377C (enExample)
DE (1) DE60228867D1 (enExample)
DK (1) DK1442134T3 (enExample)
GB (1) GB0126887D0 (enExample)
WO (1) WO2003040391A2 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE452992T1 (de) 2004-02-04 2010-01-15 Qiagen North American Holdings Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US7790364B2 (en) * 2006-01-27 2010-09-07 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for high throughput screening of pharmacological chaperones
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN107267596A (zh) * 2009-06-15 2017-10-20 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
JP2011147403A (ja) * 2010-01-22 2011-08-04 Hitachi High-Technologies Corp 細菌検査装置及び細菌検査方法
KR101629773B1 (ko) * 2014-07-16 2016-06-13 한국과학기술원 약물 펩타이드 후보 선별 장치 및 이를 이용한 약물 펩타이드 후보 선별 방법
DK3294934T3 (da) * 2015-07-03 2020-03-23 Hunan Zonsen Peplib Biotech Co Ltd Fremgangsmåde til opbygning af peptidbibliotek og relaterede vektorer
EP3596106B1 (en) * 2017-04-26 2021-08-11 Hunan Zonsen Peplib Biotech Co., Ltd Peptide library constructing method
IL312717A (en) * 2021-11-17 2024-07-01 Nerd Bio Llc Systems and methods for real-time cellular engagement with DRUG-TARGET

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2067194C (en) * 1989-10-05 2003-03-18 Glenn Kawasaki Cell-free synthesis and isolation of novel genes and polypeptides
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5805923A (en) * 1995-05-26 1998-09-08 Sony Corporation Configurable power management system having a clock stabilization filter that can be enabled or bypassed depending upon whether a crystal or can oscillator is used
DE19545320A1 (de) * 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
ATE214104T1 (de) 1998-04-22 2002-03-15 Entpr Ie Trd As Bioresearch Ie Verfahren zur charakterisierung von nukleinsäuremolekulen, das die erzeugung von verlängerbaren aufwärtsgelegenen dns-fragmenten, die durch spaltung der nukleinsäure an einer abasischen stelle entsteht, umfasst
EP2230303B1 (en) * 1999-05-05 2013-01-16 Phylogica Limited Isolating biological modulators from biodiverse gene fragment libraries
EP1276900A2 (en) * 2000-01-11 2003-01-22 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
WO2001070947A2 (en) * 2000-03-20 2001-09-27 Maxygen, Inc. Method for generating recombinant dna molecules in complex mixtures
WO2004072239A2 (en) * 2003-02-06 2004-08-26 Verdia, Inc. Novel polypeptides with antifungal activity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Andersson, B. et al. (1994) Analytical Biochemistry. 218: 300 - 308. *
Smith, c. et al. (1993) PCR Methods and Applications. 2: 328 - 332. *

Also Published As

Publication number Publication date
WO2003040391A3 (en) 2003-11-13
EP1442134A2 (en) 2004-08-04
EP1442134B1 (en) 2008-09-10
CA2465377A1 (en) 2003-05-15
GB0126887D0 (en) 2002-01-02
CA2465377C (en) 2012-04-03
DE60228867D1 (de) 2008-10-23
JP4303112B2 (ja) 2009-07-29
US20050019773A1 (en) 2005-01-27
US20150065382A1 (en) 2015-03-05
US8852866B2 (en) 2014-10-07
WO2003040391A2 (en) 2003-05-15
JP2005508194A (ja) 2005-03-31
ATE408029T1 (de) 2008-09-15
DK1442134T3 (da) 2009-01-12

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Legal Events

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FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired
NA Applications received for extensions of time, section 223

Free format text: AN APPLICATION TO EXTEND THE TIME FROM 08 NOV 2007 TO 08 JUL 2008 IN WHICH TO PAY A RENEWAL FEE HAS BEEN FILED .

NB Applications allowed - extensions of time section 223(2)

Free format text: THE TIME IN WHICH TO PAY A RENEWAL FEE HAS BEEN EXTENDED TO 08 JUL 2008.

PC Assignment registered

Owner name: DOMAINEX LIMITED

Free format text: FORMER OWNER WAS: UNIVERSITY COLLEGE LONDON; BIRKBECK COLLEGE; THE INSTITUTE OF CANCER RESEARCH

MK14 Patent ceased section 143(a) (annual fees not paid) or expired