JP2008504825A - チトクロムｐ４５０−２ｃ９をコードする遺伝子における変異を検出する方法 - Google Patents
チトクロムｐ４５０−２ｃ９をコードする遺伝子における変異を検出する方法 Download PDFInfo
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- JP2008504825A JP2008504825A JP2007519574A JP2007519574A JP2008504825A JP 2008504825 A JP2008504825 A JP 2008504825A JP 2007519574 A JP2007519574 A JP 2007519574A JP 2007519574 A JP2007519574 A JP 2007519574A JP 2008504825 A JP2008504825 A JP 2008504825A
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
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- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US58361904P | 2004-06-30 | 2004-06-30 | |
| PCT/CA2005/000998 WO2006002525A1 (en) | 2004-06-30 | 2005-06-30 | Method of detecting mutations in the gene encoding cytochrome p450-2c9 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008504825A true JP2008504825A (ja) | 2008-02-21 |
| JP2008504825A5 JP2008504825A5 (enExample) | 2008-09-04 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007519574A Pending JP2008504825A (ja) | 2004-06-30 | 2005-06-30 | チトクロムｐ４５０−２ｃ９をコードする遺伝子における変異を検出する方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20090099030A1 (enExample) |
| EP (1) | EP1781812A4 (enExample) |
| JP (1) | JP2008504825A (enExample) |
| AU (1) | AU2005259786B2 (enExample) |
| CA (1) | CA2571817A1 (enExample) |
| WO (1) | WO2006002525A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8241813B2 (en) | 2005-04-05 | 2012-08-14 | Rolls-Royce Plc | Fuel cell arrangement |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1781811B1 (en) * | 2004-06-30 | 2010-06-23 | Luminex Molecular Diagnostics, Inc. | Method of detecting mutations in the gene encoding cytochrome p450-2d6 |
| US20080248466A1 (en) * | 2004-07-30 | 2008-10-09 | Tm Bioscience Pgx Inc. | Method Of Detecting Mutations In The Gene Encoding Cytochrome P450-2C19 |
| JPWO2008066163A1 (ja) * | 2006-11-30 | 2010-03-11 | アークレイ株式会社 | Ｃｙｐ２ｃ９遺伝子増幅用プライマーセット、それを含むｃｙｐ２ｃ９遺伝子増幅用試薬およびその用途 |
| CA2704487A1 (en) * | 2007-11-02 | 2009-06-18 | Luminex Molecular Diagnostics, Inc. | One-step target detection assay |
| WO2009128730A1 (en) * | 2008-01-25 | 2009-10-22 | Patrick Gladding | Methods and compositions for the assessment of drug response |
| CN102686728B (zh) | 2009-06-29 | 2018-09-18 | 卢米耐克斯公司 | 具有发夹构象的嵌合引物及其使用方法 |
| TWI600766B (zh) | 2012-08-09 | 2017-10-01 | 財團法人工業技術研究院 | 用於偵測一目標核苷酸序列中之一特定區域的一突變及／或多形性的套組 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000047766A1 (en) * | 1999-02-11 | 2000-08-17 | Astrazeneca Ab | Method for detecting variant nucleotides using arms multiplex amplification |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4851331A (en) * | 1986-05-16 | 1989-07-25 | Allied Corporation | Method and kit for polynucleotide assay including primer-dependant DNA polymerase |
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| US5605662A (en) * | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
| JP2002523111A (ja) * | 1998-08-28 | 2002-07-30 | サンテック モレキュラー ダイアグノスティックス エービー | 特定医薬を代謝することに対する患者の能力を測定する方法 |
| JP2001017185A (ja) * | 1999-03-19 | 2001-01-23 | Otsuka Pharmaceut Co Ltd | 薬物代謝酵素遺伝子多型の簡易検出法 |
| US6287778B1 (en) * | 1999-10-19 | 2001-09-11 | Affymetrix, Inc. | Allele detection using primer extension with sequence-coded identity tags |
| EP2327794A3 (en) * | 2001-01-25 | 2011-09-14 | TM Bioscience Corporation | Polynucleotides for use as tags and tag complements, manufacture and use thereof |
| JP4781628B2 (ja) * | 2001-12-05 | 2011-09-28 | センス プロテオミック リミテッド | 対立遺伝子改変体のためのタンパク質アレイおよびその使用 |
| DE10237691B4 (de) * | 2002-08-15 | 2010-01-28 | Biotez Berlin-Buch Gmbh Biochemisch-Technologisches Zentrum | Verfahren zum Nachweis von Einzelnukleotid-Polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens |
| WO2004025244A2 (en) * | 2002-09-16 | 2004-03-25 | Sense Proteomic Limited | Protein arrays and uses thereof |
| US20080248466A1 (en) * | 2004-07-30 | 2008-10-09 | Tm Bioscience Pgx Inc. | Method Of Detecting Mutations In The Gene Encoding Cytochrome P450-2C19 |
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- 2005-06-30 JP JP2007519574A patent/JP2008504825A/ja active Pending
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- 2005-06-30 WO PCT/CA2005/000998 patent/WO2006002525A1/en not_active Ceased
- 2005-06-30 EP EP05761962A patent/EP1781812A4/en not_active Withdrawn
- 2005-06-30 US US11/631,200 patent/US20090099030A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2000047766A1 (en) * | 1999-02-11 | 2000-08-17 | Astrazeneca Ab | Method for detecting variant nucleotides using arms multiplex amplification |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8241813B2 (en) | 2005-04-05 | 2012-08-14 | Rolls-Royce Plc | Fuel cell arrangement |
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| AU2005259786B2 (en) | 2011-03-10 |
| EP1781812A1 (en) | 2007-05-09 |
| US20090099030A1 (en) | 2009-04-16 |
| AU2005259786A1 (en) | 2006-01-12 |
| WO2006002525A1 (en) | 2006-01-12 |
| EP1781812A4 (en) | 2008-10-15 |
| CA2571817A1 (en) | 2006-01-12 |
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