JP2008503203A5 - - Google Patents
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- Publication number
- JP2008503203A5 JP2008503203A5 JP2007510879A JP2007510879A JP2008503203A5 JP 2008503203 A5 JP2008503203 A5 JP 2008503203A5 JP 2007510879 A JP2007510879 A JP 2007510879A JP 2007510879 A JP2007510879 A JP 2007510879A JP 2008503203 A5 JP2008503203 A5 JP 2008503203A5
- Authority
- JP
- Japan
- Prior art keywords
- pdx1
- growth factor
- cell culture
- retinoid
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US56629304P | 2004-04-27 | 2004-04-27 | |
| US60/566,293 | 2004-04-27 | ||
| US58656604P | 2004-07-09 | 2004-07-09 | |
| US60/586,566 | 2004-07-09 | ||
| US58794204P | 2004-07-14 | 2004-07-14 | |
| US60/587,942 | 2004-07-14 | ||
| US11/021,618 | 2004-12-23 | ||
| US11/021,618 US7510876B2 (en) | 2003-12-23 | 2004-12-23 | Definitive endoderm |
| PCT/US2005/014239 WO2005116073A2 (en) | 2004-04-27 | 2005-04-26 | Pdx1 expressing endoderm |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011147598A Division JP5669681B2 (ja) | 2004-04-27 | 2011-07-01 | Pdx1発現性内胚葉 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008503203A JP2008503203A (ja) | 2008-02-07 |
| JP2008503203A5 true JP2008503203A5 (enExample) | 2008-06-19 |
| JP4926946B2 JP4926946B2 (ja) | 2012-05-09 |
Family
ID=45319532
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007510879A Expired - Lifetime JP4926946B2 (ja) | 2004-04-27 | 2005-04-26 | Pdx1発現性内胚葉 |
| JP2011147598A Expired - Lifetime JP5669681B2 (ja) | 2004-04-27 | 2011-07-01 | Pdx1発現性内胚葉 |
| JP2014168939A Expired - Lifetime JP6158152B2 (ja) | 2004-04-27 | 2014-08-22 | Pdx1発現性内胚葉 |
| JP2015111656A Expired - Lifetime JP6265942B2 (ja) | 2004-04-27 | 2015-06-01 | Pdx1発現性内胚葉 |
| JP2017026645A Expired - Lifetime JP6605520B2 (ja) | 2004-04-27 | 2017-02-16 | Pdx1発現性内胚葉 |
| JP2018167312A Expired - Lifetime JP6709262B2 (ja) | 2004-04-27 | 2018-09-06 | Pdx1発現性内胚葉 |
Family Applications After (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011147598A Expired - Lifetime JP5669681B2 (ja) | 2004-04-27 | 2011-07-01 | Pdx1発現性内胚葉 |
| JP2014168939A Expired - Lifetime JP6158152B2 (ja) | 2004-04-27 | 2014-08-22 | Pdx1発現性内胚葉 |
| JP2015111656A Expired - Lifetime JP6265942B2 (ja) | 2004-04-27 | 2015-06-01 | Pdx1発現性内胚葉 |
| JP2017026645A Expired - Lifetime JP6605520B2 (ja) | 2004-04-27 | 2017-02-16 | Pdx1発現性内胚葉 |
| JP2018167312A Expired - Lifetime JP6709262B2 (ja) | 2004-04-27 | 2018-09-06 | Pdx1発現性内胚葉 |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1740612B1 (enExample) |
| JP (6) | JP4926946B2 (enExample) |
| KR (2) | KR101395516B1 (enExample) |
| CN (1) | CN103103158B (enExample) |
| AU (1) | AU2005247869B2 (enExample) |
| CA (1) | CA2564114C (enExample) |
| DK (2) | DK2377922T3 (enExample) |
| EA (1) | EA011953B1 (enExample) |
| ES (2) | ES2751095T3 (enExample) |
| IL (1) | IL178847A (enExample) |
| MX (1) | MXPA06012445A (enExample) |
| NZ (1) | NZ550605A (enExample) |
| SG (1) | SG152273A1 (enExample) |
| WO (1) | WO2005116073A2 (enExample) |
Families Citing this family (82)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| US8647873B2 (en) | 2004-04-27 | 2014-02-11 | Viacyte, Inc. | PDX1 expressing endoderm |
| WO2007059007A2 (en) | 2005-11-14 | 2007-05-24 | Cythera, Inc. | Markers of definitive endoderm |
| US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| US7985585B2 (en) | 2004-07-09 | 2011-07-26 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
| US7541185B2 (en) | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
| KR101221302B1 (ko) | 2003-12-23 | 2013-01-11 | 비아싸이트, 인크. | 완전 내배엽 |
| AU2005272078B2 (en) * | 2004-07-09 | 2011-04-14 | Viacyte, Inc. | Methods for identifying factors for differentiating definitive endoderm |
| EP1791952A4 (en) | 2004-08-13 | 2008-06-11 | Univ Georgia Res Found | COMPOSITIONS AND METHODS OF SELF-RENEWAL AND DIFFERENTIATION IN HUMAN EMBRYONAL STEM CELLS |
| US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
| AU2005322007B2 (en) * | 2004-12-23 | 2011-06-30 | Viacyte, Inc. | Expansion of definitive endoderm cells |
| JP2008528038A (ja) * | 2005-01-31 | 2008-07-31 | エス セル インターナショナル ピーティーイー リミテッド | 胚性幹細胞の指示された分化及びその利用 |
| CN101282652B (zh) * | 2005-06-02 | 2013-09-11 | 亚利桑那董事会(代表亚利桑那大学) | 预血管化的装置及相关方法 |
| CA2613889A1 (en) | 2005-06-08 | 2006-12-14 | Centocor, Inc. | A cellular therapy for ocular degeneration |
| AU2012202481B2 (en) * | 2005-10-27 | 2015-07-02 | Viacyte, Inc. | PDX1-expressing dorsal and ventral foregut endoderm |
| EP3584311A1 (en) * | 2005-10-27 | 2019-12-25 | Viacyte, Inc. | Pdx-1 expressing dorsal and ventral foregut endoderm |
| EP1994141B1 (en) | 2006-02-23 | 2017-11-15 | ViaCyte, Inc. | Compositions and methods useful for culturing differentiable cells |
| CA2644468C (en) * | 2006-03-02 | 2022-02-01 | Cythera, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| US11254916B2 (en) | 2006-03-02 | 2022-02-22 | Viacyte, Inc. | Methods of making and using PDX1-positive pancreatic endoderm cells |
| US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| EP2021462B1 (en) * | 2006-04-28 | 2019-01-09 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
| US7989204B2 (en) | 2006-04-28 | 2011-08-02 | Viacyte, Inc. | Hepatocyte lineage cells |
| US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| CA3114827C (en) | 2007-07-31 | 2023-09-05 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic endocrine |
| US7695963B2 (en) | 2007-09-24 | 2010-04-13 | Cythera, Inc. | Methods for increasing definitive endoderm production |
| CA2954431C (en) * | 2007-11-27 | 2021-08-24 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic cells |
| EP2227487A1 (en) | 2007-12-28 | 2010-09-15 | Novo Nordisk A/S | Ddr1-mediated cell purification of pancreatic endocrine cells through their progenitors |
| RU2551772C2 (ru) | 2008-02-21 | 2015-05-27 | Сентокор Орто Байотек Инк. | Способы, поверхностно-модифицированные носители и композиции для иммобилизации, культивирования и открепления клеток |
| JP2011518547A (ja) | 2008-04-03 | 2011-06-30 | ノボ・ノルデイスク・エー/エス | 膵臓内分泌プレ前駆体細胞及びその子孫のdner媒介細胞精製 |
| US8623648B2 (en) | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
| US7939322B2 (en) | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
| AU2009267137A1 (en) | 2008-06-30 | 2010-01-07 | Centocor Ortho Biotech Inc. | Differentiation of pluripotent stem cells |
| MX2011000123A (es) * | 2008-06-30 | 2011-02-25 | Centocor Ortho Biotech Inc | Diferenciacion de las celulas madre pluripotentes. |
| MX2011004565A (es) | 2008-10-31 | 2011-07-28 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico. |
| MX2011004563A (es) | 2008-10-31 | 2011-06-01 | Centocor Ortho Biotech Inc | Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico. |
| US8008075B2 (en) | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| US8895300B2 (en) | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
| EP3363444B1 (en) | 2008-11-14 | 2022-09-14 | ViaCyte, Inc. | Encapsulation of pancreatic cells derived from human pluripotent stem cells |
| EP3260534A1 (en) | 2008-11-20 | 2017-12-27 | Janssen Biotech, Inc. | Pluripotent stem cell culture on micro-carriers |
| BRPI0921996A2 (pt) | 2008-11-20 | 2015-08-18 | Centocor Ortho Biotech Inc | Métodos e composições para cultura e ligação de células em substratos planos. |
| KR101785626B1 (ko) | 2009-07-20 | 2017-10-16 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| EP2456862A4 (en) | 2009-07-20 | 2013-02-27 | Janssen Biotech Inc | DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS |
| KR101786735B1 (ko) | 2009-07-20 | 2017-10-18 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| BR112012017761A2 (pt) | 2009-12-23 | 2015-09-15 | Centocor Ortho Biotech Inc | diferenciação das células-tronco embrionárias humanas |
| US12441985B2 (en) | 2009-12-23 | 2025-10-14 | Janssen Biotech, Inc. | Methods for making insulin in vivo |
| PH12012501254A1 (en) | 2009-12-23 | 2012-11-05 | Janssen Biotech Inc | Differentiation of human embryonic stem cells |
| US9969981B2 (en) | 2010-03-01 | 2018-05-15 | Janssen Biotech, Inc. | Methods for purifying cells derived from pluripotent stem cells |
| US9719068B2 (en) | 2010-05-06 | 2017-08-01 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| RU2663339C1 (ru) | 2010-05-12 | 2018-08-03 | Янссен Байотек, Инк. | Дифференцирование эмбриональных стволовых клеток человека |
| KR101836850B1 (ko) | 2010-08-31 | 2018-03-09 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| CA2809300A1 (en) | 2010-08-31 | 2012-03-08 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| JP6133776B2 (ja) | 2010-08-31 | 2017-05-24 | ヤンセン バイオテツク,インコーポレーテツド | 多能性幹細胞の分化 |
| JP5888852B2 (ja) * | 2010-12-08 | 2016-03-22 | 学校法人近畿大学 | 免疫不全動物を用いた細胞の製法 |
| JP2012143229A (ja) * | 2010-12-24 | 2012-08-02 | Chiba Univ | 多能性幹細胞培養用組成物およびその用途 |
| ES2902650T3 (es) | 2011-06-21 | 2022-03-29 | Novo Nordisk As | Inducción eficiente de endodermo definitivo a partir de células madre pluripotentes |
| AU2012355698B2 (en) | 2011-12-22 | 2018-11-29 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
| RU2018128383A (ru) | 2012-03-07 | 2019-03-14 | Янссен Байотек, Инк. | Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток |
| RU2018108850A (ru) | 2012-06-08 | 2019-02-26 | Янссен Байотек, Инк. | Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки |
| US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
| SG10201707811XA (en) | 2012-12-31 | 2017-11-29 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators |
| MX2015008577A (es) | 2012-12-31 | 2015-09-07 | Janssen Biotech Inc | Cultivo de celulas madre embrionarias humanas en la interfase aire-liquido para la diferenciacion en celulas endocrinas pancreaticas. |
| EP4039798A1 (en) | 2012-12-31 | 2022-08-10 | Janssen Biotech, Inc. | Suspension and clustering of human pluripotent cells |
| KR102162138B1 (ko) | 2014-05-16 | 2020-10-06 | 얀센 바이오테크 인코포레이티드 | 췌장 내분비 세포에서 mafa 발현을 향상시키기 위한 소분자의 용도 |
| EP3149156B1 (en) * | 2014-05-28 | 2021-02-17 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
| JP6804438B2 (ja) | 2014-10-17 | 2020-12-23 | チルドレンズ ホスピタル メディカル センター | 多能性幹細胞を使用するヒト小腸のin vivoモデル、並びにそれを作製、及び使用する方法 |
| US10494608B2 (en) | 2015-04-24 | 2019-12-03 | University Of Copenhagen | Isolation of bona fide pancreatic progenitor cells |
| MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
| EP4177335A1 (en) | 2016-05-05 | 2023-05-10 | Children's Hospital Medical Center | Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same |
| EP3534976A4 (en) | 2016-11-04 | 2020-09-16 | Children's Hospital Medical Center | PATHOLOGICAL MODELS OF HEPATIC ORGANOIDS AND ASSOCIATED METHODS OF MANUFACTURE AND USE |
| EP3548507A4 (en) | 2016-12-05 | 2020-07-15 | Children's Hospital Medical Center | COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF |
| EP3609997A4 (en) | 2017-04-14 | 2021-03-03 | Children's Hospital Medical Center | COMPOSITIONS OF STEM CELLS FROM MULTIPLE DONOR CELLS AND THEIR PREPARATION PROCEDURES |
| EP3694603A4 (en) | 2017-10-10 | 2021-07-14 | Children's Hospital Medical Center | ESOPHAGUS TISSUE AND / OR ORGANOID COMPOSITIONS AND METHOD FOR MANUFACTURING THEREOF |
| CA2983845C (en) | 2017-10-26 | 2024-01-30 | University Of Copenhagen | Generation of glucose-responsive beta cells |
| US12379372B2 (en) | 2017-12-21 | 2025-08-05 | Children's Hospital Medical Center | Digitalized human organoids and methods of using same |
| KR102887406B1 (ko) | 2018-07-26 | 2025-11-19 | 칠드런즈 호스피탈 메디칼 센터 | 간-담도-췌장 조직 및 이를 제조하는 방법 |
| WO2020056158A1 (en) | 2018-09-12 | 2020-03-19 | Children's Hospital Medical Center | Organoid compositions for the production of hematopoietic stem cells and derivatives thereof |
| AU2020283056B2 (en) | 2019-05-31 | 2023-06-08 | Viacyte, Inc. | A biocompatible membrane composite |
| EP3976237A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
| EP3975926A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates, Inc. | A biocompatible membrane composite |
| CA3139591C (en) | 2019-05-31 | 2024-01-16 | Timothy M. BRUHN | A biocompatible membrane composite |
| CA3190604A1 (en) | 2020-08-15 | 2022-02-24 | Regeneron Pharmaceuticals, Inc. | Treatment of obesity in subjects having variant nucleic acid molecules encoding calcitonin receptor (calcr) |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3945846B2 (ja) * | 1995-11-09 | 2007-07-18 | 武田薬品工業株式会社 | 膵臓機能改善剤 |
| US6331406B1 (en) * | 1997-03-31 | 2001-12-18 | The John Hopkins University School Of Medicine | Human enbryonic germ cell and methods of use |
| EP1175487A2 (en) * | 1999-02-10 | 2002-01-30 | Curis, Inc. | Pancreatic progenitor cells, methods and uses related thereto |
| JP4426064B2 (ja) * | 2000-05-31 | 2010-03-03 | 独立行政法人科学技術振興機構 | 脊椎動物の膵臓のインビトロ形成方法 |
| WO2002002750A1 (en) * | 2000-06-30 | 2002-01-10 | Amcyte, Inc. | Culturing pancreatic stem cells having a specified, intermediate stage of development |
| AU8617301A (en) * | 2000-08-01 | 2002-02-13 | Yissum Res Dev Co | Directed differentiation of embryonic cells |
| US20020168763A1 (en) * | 2000-11-30 | 2002-11-14 | Yan Wen Liang | Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same |
| EP1366148A2 (en) * | 2001-01-24 | 2003-12-03 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE DEPARTMENT OF HEALTH & HUMAN SERVICES | Differentiation of stem cells to pancreatic endocrine cells |
| EP1393066A4 (en) * | 2001-05-15 | 2006-01-25 | Rappaport Family Inst For Res | INSULIN-PRODUCING CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS |
| CA2451838A1 (en) * | 2001-05-25 | 2002-12-05 | Cythera, Inc. | Stem cell differentiation |
| WO2002102982A2 (en) * | 2001-06-14 | 2002-12-27 | Regents Of The University Of California | Hoxd3 compositions ad methods for improved wound healing |
| EP1298201A1 (en) * | 2001-09-27 | 2003-04-02 | Cardion AG | Process for the production of cells exhibiting an islet-beta-cell-like state |
| WO2003046141A2 (en) * | 2001-11-26 | 2003-06-05 | Advanced Cell Technology, Inc. | Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells |
| GB2399823B (en) * | 2001-12-07 | 2006-02-15 | Geron Corp | Islet cells from primate pluripotent stem cells |
| US20030138949A1 (en) * | 2001-12-12 | 2003-07-24 | Anil Bhushan | Methods for the regeneration of pancreatic islets and expansion of pancreatic endocrine cells |
| US20030162290A1 (en) * | 2002-01-25 | 2003-08-28 | Kazutomo Inoue | Method for inducing differentiation of embryonic stem cells into functioning cells |
| WO2003089619A2 (en) * | 2002-04-19 | 2003-10-30 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Placental derived stem cells and uses thereof |
| EP1516925A1 (en) * | 2003-09-18 | 2005-03-23 | Fundacion IVI para el Estudio de la reproduccion Humana (FIVIER) | Generation of human embryonic stem cells from triploid zygotes |
| CA2555571C (en) * | 2004-04-01 | 2012-10-16 | Wisconsin Alumni Research Foundation | Differentiation of stem cells to endoderm and pancreatic lineage |
-
2005
- 2005-04-26 ES ES05780039T patent/ES2751095T3/es not_active Expired - Lifetime
- 2005-04-26 CN CN201210431212.6A patent/CN103103158B/zh not_active Expired - Lifetime
- 2005-04-26 WO PCT/US2005/014239 patent/WO2005116073A2/en not_active Ceased
- 2005-04-26 SG SG200902871-3A patent/SG152273A1/en unknown
- 2005-04-26 KR KR1020107004283A patent/KR101395516B1/ko not_active Expired - Lifetime
- 2005-04-26 CA CA2564114A patent/CA2564114C/en not_active Expired - Lifetime
- 2005-04-26 JP JP2007510879A patent/JP4926946B2/ja not_active Expired - Lifetime
- 2005-04-26 AU AU2005247869A patent/AU2005247869B2/en not_active Expired
- 2005-04-26 EA EA200601995A patent/EA011953B1/ru not_active IP Right Cessation
- 2005-04-26 DK DK10012385.0T patent/DK2377922T3/da active
- 2005-04-26 DK DK05780039.3T patent/DK1740612T3/da active
- 2005-04-26 KR KR1020067022588A patent/KR101278421B1/ko not_active Expired - Lifetime
- 2005-04-26 ES ES10012385T patent/ES2801001T3/es not_active Expired - Lifetime
- 2005-04-26 EP EP05780039.3A patent/EP1740612B1/en not_active Expired - Lifetime
- 2005-04-26 NZ NZ550605A patent/NZ550605A/en not_active IP Right Cessation
- 2005-04-26 MX MXPA06012445A patent/MXPA06012445A/es active IP Right Grant
-
2006
- 2006-10-24 IL IL178847A patent/IL178847A/en active IP Right Grant
-
2011
- 2011-07-01 JP JP2011147598A patent/JP5669681B2/ja not_active Expired - Lifetime
-
2014
- 2014-08-22 JP JP2014168939A patent/JP6158152B2/ja not_active Expired - Lifetime
-
2015
- 2015-06-01 JP JP2015111656A patent/JP6265942B2/ja not_active Expired - Lifetime
-
2017
- 2017-02-16 JP JP2017026645A patent/JP6605520B2/ja not_active Expired - Lifetime
-
2018
- 2018-09-06 JP JP2018167312A patent/JP6709262B2/ja not_active Expired - Lifetime
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