JP2008503203A5 - - Google Patents
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- JP2008503203A5 JP2008503203A5 JP2007510879A JP2007510879A JP2008503203A5 JP 2008503203 A5 JP2008503203 A5 JP 2008503203A5 JP 2007510879 A JP2007510879 A JP 2007510879A JP 2007510879 A JP2007510879 A JP 2007510879A JP 2008503203 A5 JP2008503203 A5 JP 2008503203A5
- Authority
- JP
- Japan
- Prior art keywords
- pdx1
- growth factor
- cell culture
- retinoid
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004027 cells Anatomy 0.000 claims 10
- 102100009419 PDHX Human genes 0.000 claims 9
- 101710014878 PDHX Proteins 0.000 claims 9
- 101700077314 PDX1 Proteins 0.000 claims 9
- 239000003102 growth factor Substances 0.000 claims 8
- 238000004113 cell culture Methods 0.000 claims 7
- SHGAZHPCJJPHSC-NWVFGJFESA-N Tretinoin Chemical compound OC(=O)/C=C(\C)/C=C/C=C(C)C=CC1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-NWVFGJFESA-N 0.000 claims 6
- 229960001727 Tretinoin Drugs 0.000 claims 6
- 210000004039 endoderm cell Anatomy 0.000 claims 6
- 229930002330 retinoic acid Natural products 0.000 claims 6
- 150000004492 retinoid derivatives Chemical class 0.000 claims 5
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 3
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 3
- 102100000261 FGF10 Human genes 0.000 claims 2
- 101700052889 FGF10 Proteins 0.000 claims 2
- 102000013529 alpha-Fetoproteins Human genes 0.000 claims 2
- 108010026331 alpha-Fetoproteins Proteins 0.000 claims 2
- 102100015787 SOX7 Human genes 0.000 claims 1
- 101700036900 SOX7 Proteins 0.000 claims 1
- 102100012196 ZIC1 Human genes 0.000 claims 1
- 101700055950 ZIC1 Proteins 0.000 claims 1
- 108010023082 activin A Proteins 0.000 claims 1
- 238000003365 immunocytochemistry Methods 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
Claims (18)
- ＰＤＸ１陽性内胚葉細胞を作製する方法であって、
外因性ＴＧＦβスーパーファミリー増殖因子の存在下で、多分化能ヒト細胞の集団を培養する工程；
該細胞集団から外因性ＴＧＦβスーパーファミリー増殖因子を取り除く工程；および
レチノイドの存在下で該細胞集団を連続培養し、多分化能ＰＤＸ１陽性内胚葉細胞を作製する工程、
を包含する、方法。 - 前記細胞集団を連続培養する工程が、ＦＧＦファミリー増殖因子を該細胞集団に供給する工程を包含する、請求項１に記載の方法。
- 前記ＦＧＦファミリー増殖因子は、前記レチノイドとほぼ同時に供給される請求項２に記載の方法。
- 前記ＦＧＦファミリー増殖因子がＦＧＦ−１０を含む、請求項２または３に記載の方法。
- 前記細胞集団の少なくとも２５％がＰＤＸ１を発現する、請求項１〜４のいずれか１項に記載の方法。
- 定量ポリメラーゼ連鎖反応によって決定される場合、前記ＰＤＸ１陽性内胚葉細胞において、ＰＤＸ１の発現が、α−フェトプロテイン（ＡＦＰ）、ＳＯＸ７、ＳＯＸ１、ＺＩＣ１およびＮＦＭからなる群から選択されるマーカーの発現より高い請求項１〜５のいずれか１項に記載の方法。
- 前記ＰＤＸ１の発現が、免疫細胞化学により決定される請求項１〜６のいずれか１項に記載の方法。
- 前記レチノイドは、レチノイン酸（ＲＡ）である請求項１〜７のいずれか１項に記載の方法。
- ＲＡは、０．０１μＭから約５０μＭまでの範囲の濃度で供給される請求項８に記載の方法。
- ＲＡは、０．１μＭから約１０μＭまでの範囲の濃度で供給される請求項８に記載の方法。
- 前記外因性ＴＧＦβスーパーファミリー増殖因子がアクチビンＡを含む、請求項１〜１０のいずれか１項に記載の方法。
- ヒト細胞の少なくとも約１０％がＰＤＸ１陽性内胚葉細胞である、ヒト細胞を含むインビトロ細胞培養物。
- 前記ヒト細胞の少なくとも約２５％が、ＰＤＸ１陽性内胚葉細胞である請
求項１２に記載の細胞培養物。 - レチノイドをさらに含む請求項１２または１３に記載の細胞培養物。
- 前記レチノイドがレチノイン酸（ＲＡ）を含む、請求項１４に記載の細胞培養物。
- ＦＧＦファミリー増殖因子をさらに含む、請求項１２〜１５のいずれか１項に記載の細胞培養物。
- 前記ＦＧＦファミリー増殖因子がＦＧＦ−１０を含む、請求項１６に記載の細胞培養物。
- 前記ヒトＰＤＸ１陽性内胚葉細胞が非組換え細胞である、請求項１２〜１７のいずれか１項に記載の細胞培養物。
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US56629304P | 2004-04-27 | 2004-04-27 | |
| US60/566,293 | 2004-04-27 | ||
| US58656604P | 2004-07-09 | 2004-07-09 | |
| US60/586,566 | 2004-07-09 | ||
| US58794204P | 2004-07-14 | 2004-07-14 | |
| US60/587,942 | 2004-07-14 | ||
| US11/021,618 US7510876B2 (en) | 2003-12-23 | 2004-12-23 | Definitive endoderm |
| US11/021,618 | 2004-12-23 | ||
| PCT/US2005/014239 WO2005116073A2 (en) | 2004-04-27 | 2005-04-26 | Pdx1 expressing endoderm |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011147598A Division JP5669681B2 (ja) | 2004-04-27 | 2011-07-01 | Ｐｄｘ１発現性内胚葉 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008503203A JP2008503203A (ja) | 2008-02-07 |
| JP2008503203A5 true JP2008503203A5 (ja) | 2008-06-19 |
| JP4926946B2 JP4926946B2 (ja) | 2012-05-09 |
Family
ID=45319532
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007510879A Active JP4926946B2 (ja) | 2004-04-27 | 2005-04-26 | Ｐｄｘ１発現性内胚葉 |
| JP2011147598A Active JP5669681B2 (ja) | 2004-04-27 | 2011-07-01 | Ｐｄｘ１発現性内胚葉 |
| JP2014168939A Active JP6158152B2 (ja) | 2004-04-27 | 2014-08-22 | Ｐｄｘ１発現性内胚葉 |
| JP2015111656A Active JP6265942B2 (ja) | 2004-04-27 | 2015-06-01 | Ｐｄｘ１発現性内胚葉 |
| JP2017026645A Active JP6605520B2 (ja) | 2004-04-27 | 2017-02-16 | Ｐｄｘ１発現性内胚葉 |
| JP2018167312A Active JP6709262B2 (ja) | 2004-04-27 | 2018-09-06 | Ｐｄｘ１発現性内胚葉 |
Family Applications After (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011147598A Active JP5669681B2 (ja) | 2004-04-27 | 2011-07-01 | Ｐｄｘ１発現性内胚葉 |
| JP2014168939A Active JP6158152B2 (ja) | 2004-04-27 | 2014-08-22 | Ｐｄｘ１発現性内胚葉 |
| JP2015111656A Active JP6265942B2 (ja) | 2004-04-27 | 2015-06-01 | Ｐｄｘ１発現性内胚葉 |
| JP2017026645A Active JP6605520B2 (ja) | 2004-04-27 | 2017-02-16 | Ｐｄｘ１発現性内胚葉 |
| JP2018167312A Active JP6709262B2 (ja) | 2004-04-27 | 2018-09-06 | Ｐｄｘ１発現性内胚葉 |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP1740612B1 (ja) |
| JP (6) | JP4926946B2 (ja) |
| KR (2) | KR101395516B1 (ja) |
| CN (1) | CN103103158B (ja) |
| AU (1) | AU2005247869B2 (ja) |
| CA (1) | CA2564114C (ja) |
| DK (2) | DK2377922T3 (ja) |
| EA (1) | EA011953B1 (ja) |
| ES (2) | ES2801001T3 (ja) |
| IL (1) | IL178847A (ja) |
| MX (1) | MXPA06012445A (ja) |
| NZ (1) | NZ550605A (ja) |
| SG (1) | SG152273A1 (ja) |
| WO (1) | WO2005116073A2 (ja) |
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| AU2002312514A1 (en) * | 2001-06-14 | 2003-01-02 | Regents Of The University Of California | Hoxd3 compositions ad methods for improved wound healing |
| EP1298201A1 (en) * | 2001-09-27 | 2003-04-02 | Cardion AG | Process for the production of cells exhibiting an islet-beta-cell-like state |
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| EP1730268A2 (en) * | 2004-04-01 | 2006-12-13 | Wisconsin Alumni Research Foundation | Differentiation of stem cells to endoderm and pancreatic lineage |
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