JP2008161138A5 - - Google Patents
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- Publication number
- JP2008161138A5 JP2008161138A5 JP2006355952A JP2006355952A JP2008161138A5 JP 2008161138 A5 JP2008161138 A5 JP 2008161138A5 JP 2006355952 A JP2006355952 A JP 2006355952A JP 2006355952 A JP2006355952 A JP 2006355952A JP 2008161138 A5 JP2008161138 A5 JP 2008161138A5
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- cell
- activation solution
- hydrogen bond
- cleaving agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000427 antigen Substances 0.000 claims 16
- 102000036639 antigens Human genes 0.000 claims 16
- 108091007433 antigens Proteins 0.000 claims 16
- 239000000243 solution Substances 0.000 claims 11
- 230000004913 activation Effects 0.000 claims 10
- 239000003795 chemical substances by application Substances 0.000 claims 5
- 229910052739 hydrogen Inorganic materials 0.000 claims 5
- 239000001257 hydrogen Substances 0.000 claims 5
- 238000000034 method Methods 0.000 claims 3
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical group OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 claims 2
- 230000003213 activating effect Effects 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000002738 chelating agent Substances 0.000 claims 2
- 238000004132 cross linking Methods 0.000 claims 2
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical group OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims 2
- 239000007996 HEPPS buffer Substances 0.000 claims 1
- 239000012190 activator Substances 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 239000000834 fixative Substances 0.000 claims 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims 1
- 238000012744 immunostaining Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006355952A JP5241100B2 (ja) | 2006-12-28 | 2006-12-28 | 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 |
| EP07150313A EP1975615B1 (en) | 2006-12-28 | 2007-12-21 | Method for detecting a cell as well as use of an agent for breaking a hydrogen bond |
| AT07150313T ATE447182T1 (de) | 2006-12-28 | 2007-12-21 | Verfahren zur zellerkennung sowie verwendung eines wirkstoffs zum durchbrechen einer wasserstoffbindung |
| DE602007002981T DE602007002981D1 (de) | 2006-12-28 | 2007-12-21 | Verfahren zur Zellerkennung sowie Verwendung eines Wirkstoffs zum Durchbrechen einer Wasserstoffbindung |
| US11/966,318 US7871783B2 (en) | 2006-12-28 | 2007-12-28 | Method for activating an antigen, method for detecting a cell, and solution for activating an antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006355952A JP5241100B2 (ja) | 2006-12-28 | 2006-12-28 | 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008161138A JP2008161138A (ja) | 2008-07-17 |
| JP2008161138A5 true JP2008161138A5 (enExample) | 2010-01-21 |
| JP5241100B2 JP5241100B2 (ja) | 2013-07-17 |
Family
ID=39333119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006355952A Expired - Fee Related JP5241100B2 (ja) | 2006-12-28 | 2006-12-28 | 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7871783B2 (enExample) |
| EP (1) | EP1975615B1 (enExample) |
| JP (1) | JP5241100B2 (enExample) |
| AT (1) | ATE447182T1 (enExample) |
| DE (1) | DE602007002981D1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009277638A1 (en) * | 2008-07-30 | 2010-02-04 | Sysmex Corporation | Reagent for detecting abnormal cell in cervix of uterus, and method for detecting abnormal cell in cervix of uterus by using same |
| CN108061677B (zh) * | 2010-03-12 | 2021-10-29 | 国立研究开发法人理化学研究所 | 用于生物材料的澄清试剂和其用途 |
| EP2711682B1 (en) | 2011-05-20 | 2019-07-10 | Riken | Clarifying reagent for biological materials and use thereof |
| JP6433901B2 (ja) | 2013-08-14 | 2018-12-05 | 国立研究開発法人理化学研究所 | 光透過性に優れた生物材料を調製するための組成物およびその利用 |
| CN111929122B (zh) * | 2020-06-29 | 2022-10-28 | 广州江元医疗科技有限公司 | 一种用于免疫细胞化学染色的抗原修复方法、细胞悬液及应用该方法的化学染色方法 |
| CN116298253A (zh) * | 2023-03-30 | 2023-06-23 | 河北明志康华生物科技有限公司 | 一种膀胱癌标记物核基质蛋白Nmp22的检测 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5424000A (en) * | 1993-10-05 | 1995-06-13 | West Agro, Inc. | Acid cleanings and staining compositions |
| BR9814271A (pt) * | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
| US6544798B1 (en) * | 1999-02-26 | 2003-04-08 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
| US6592821B1 (en) * | 1999-05-17 | 2003-07-15 | Caliper Technologies Corp. | Focusing of microparticles in microfluidic systems |
| FR2807652A1 (fr) * | 2000-04-18 | 2001-10-19 | Oreal | Composition pour la teinture d'oxydation des fibres comprenant une 1-(4-aminophenyl)-pyrrolidine et un polymere cationique |
| JP3797418B2 (ja) * | 2001-05-29 | 2006-07-19 | 茂樹 並松 | 抗原賦活化法及びその抗原賦活剤 |
| US20040260157A1 (en) * | 2003-06-20 | 2004-12-23 | Montes Miguel A. | Method for automated screening of cervical/endocervical malignant and premalignant epithelial lesions using flow cytometry with HPV DNA fluorescent in-situ hybridization ( FISH) technology |
| US8632994B2 (en) * | 2003-12-01 | 2014-01-21 | Dako Denmark A/S | Methods and compositions for immuno-histochemical detection |
| SI1924252T1 (sl) * | 2005-07-28 | 2011-12-30 | Id Fish Technology Inc | Metoda za izboljšanje celične permeabilnosti za tuje partikle |
-
2006
- 2006-12-28 JP JP2006355952A patent/JP5241100B2/ja not_active Expired - Fee Related
-
2007
- 2007-12-21 EP EP07150313A patent/EP1975615B1/en not_active Not-in-force
- 2007-12-21 AT AT07150313T patent/ATE447182T1/de not_active IP Right Cessation
- 2007-12-21 DE DE602007002981T patent/DE602007002981D1/de active Active
- 2007-12-28 US US11/966,318 patent/US7871783B2/en active Active
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