JP5241100B2 - 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 - Google Patents
抗原賦活化液、抗原賦活化方法及び細胞の検出方法 Download PDFInfo
- Publication number
- JP5241100B2 JP5241100B2 JP2006355952A JP2006355952A JP5241100B2 JP 5241100 B2 JP5241100 B2 JP 5241100B2 JP 2006355952 A JP2006355952 A JP 2006355952A JP 2006355952 A JP2006355952 A JP 2006355952A JP 5241100 B2 JP5241100 B2 JP 5241100B2
- Authority
- JP
- Japan
- Prior art keywords
- antigen
- cells
- cell
- antigen activation
- activation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000427 antigen Substances 0.000 title claims abstract description 157
- 102000036639 antigens Human genes 0.000 title claims abstract description 157
- 108091007433 antigens Proteins 0.000 title claims abstract description 157
- 230000004913 activation Effects 0.000 title claims abstract description 92
- 238000000034 method Methods 0.000 title claims abstract description 35
- 239000007788 liquid Substances 0.000 title claims description 27
- 238000001514 detection method Methods 0.000 title claims description 11
- 230000003213 activating effect Effects 0.000 claims abstract description 11
- 238000012744 immunostaining Methods 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 71
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 46
- 239000004202 carbamide Substances 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 21
- 239000007791 liquid phase Substances 0.000 claims description 19
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical group OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims description 10
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical group OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 5
- 239000007996 HEPPS buffer Substances 0.000 claims description 4
- 239000002738 chelating agent Substances 0.000 claims description 4
- 239000012190 activator Substances 0.000 claims description 3
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 21
- 239000001257 hydrogen Substances 0.000 abstract description 21
- 239000003795 chemical substances by application Substances 0.000 abstract description 12
- 210000004027 cell Anatomy 0.000 description 164
- 238000001994 activation Methods 0.000 description 78
- 238000010186 staining Methods 0.000 description 32
- 239000000834 fixative Substances 0.000 description 18
- 210000003679 cervix uteri Anatomy 0.000 description 15
- 239000012071 phase Substances 0.000 description 12
- 239000000356 contaminant Substances 0.000 description 11
- 239000006228 supernatant Substances 0.000 description 10
- 238000004132 cross linking Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 239000011521 glass Substances 0.000 description 9
- 210000004940 nucleus Anatomy 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 230000003248 secreting effect Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 6
- 239000000975 dye Substances 0.000 description 6
- 206010008342 Cervix carcinoma Diseases 0.000 description 5
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 5
- 201000010881 cervical cancer Diseases 0.000 description 5
- 210000003743 erythrocyte Anatomy 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 239000012192 staining solution Substances 0.000 description 5
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- 229920001213 Polysorbate 20 Polymers 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 4
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000035752 proliferative phase Effects 0.000 description 4
- 210000004085 squamous epithelial cell Anatomy 0.000 description 4
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000007995 HEPES buffer Substances 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 2
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 102000008297 Nuclear Matrix-Associated Proteins Human genes 0.000 description 2
- 108010035916 Nuclear Matrix-Associated Proteins Proteins 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- 208000002151 Pleural effusion Diseases 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 210000000941 bile Anatomy 0.000 description 2
- 239000006172 buffering agent Substances 0.000 description 2
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- LVQFQZZGTZFUNF-UHFFFAOYSA-N 2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazine-1,4-diium-1-yl]propane-1-sulfonate Chemical compound OS(=O)(=O)CC(O)CN1CCN(CC(O)CS(O)(=O)=O)CC1 LVQFQZZGTZFUNF-UHFFFAOYSA-N 0.000 description 1
- HQRZATHKXBDMFT-UHFFFAOYSA-N 2-hydroxypropane-1-sulfonic acid dihydrate Chemical compound O.O.CC(O)CS(O)(=O)=O HQRZATHKXBDMFT-UHFFFAOYSA-N 0.000 description 1
- AYKYXWQEBUNJCN-UHFFFAOYSA-N 3-methylfuran-2,5-dione Chemical compound CC1=CC(=O)OC1=O AYKYXWQEBUNJCN-UHFFFAOYSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- HLFSDGLLUJUHTE-SNVBAGLBSA-N Levamisole Chemical compound C1([C@H]2CN3CCSC3=N2)=CC=CC=C1 HLFSDGLLUJUHTE-SNVBAGLBSA-N 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 229960004308 acetylcysteine Drugs 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 150000001483 arginine derivatives Chemical class 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940120503 dihydroxyacetone Drugs 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- KAKKHKRHCKCAGH-UHFFFAOYSA-L disodium;(4-nitrophenyl) phosphate;hexahydrate Chemical compound O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)C1=CC=C(OP([O-])([O-])=O)C=C1 KAKKHKRHCKCAGH-UHFFFAOYSA-L 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 210000004696 endometrium Anatomy 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 150000002357 guanidines Chemical class 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229960001614 levamisole Drugs 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 229960004025 sodium salicylate Drugs 0.000 description 1
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- -1 thiol compounds Chemical class 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Battery Electrode And Active Subsutance (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006355952A JP5241100B2 (ja) | 2006-12-28 | 2006-12-28 | 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 |
| EP07150313A EP1975615B1 (en) | 2006-12-28 | 2007-12-21 | Method for detecting a cell as well as use of an agent for breaking a hydrogen bond |
| AT07150313T ATE447182T1 (de) | 2006-12-28 | 2007-12-21 | Verfahren zur zellerkennung sowie verwendung eines wirkstoffs zum durchbrechen einer wasserstoffbindung |
| DE602007002981T DE602007002981D1 (de) | 2006-12-28 | 2007-12-21 | Verfahren zur Zellerkennung sowie Verwendung eines Wirkstoffs zum Durchbrechen einer Wasserstoffbindung |
| US11/966,318 US7871783B2 (en) | 2006-12-28 | 2007-12-28 | Method for activating an antigen, method for detecting a cell, and solution for activating an antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006355952A JP5241100B2 (ja) | 2006-12-28 | 2006-12-28 | 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008161138A JP2008161138A (ja) | 2008-07-17 |
| JP2008161138A5 JP2008161138A5 (enExample) | 2010-01-21 |
| JP5241100B2 true JP5241100B2 (ja) | 2013-07-17 |
Family
ID=39333119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006355952A Expired - Fee Related JP5241100B2 (ja) | 2006-12-28 | 2006-12-28 | 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7871783B2 (enExample) |
| EP (1) | EP1975615B1 (enExample) |
| JP (1) | JP5241100B2 (enExample) |
| AT (1) | ATE447182T1 (enExample) |
| DE (1) | DE602007002981D1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009277638A1 (en) * | 2008-07-30 | 2010-02-04 | Sysmex Corporation | Reagent for detecting abnormal cell in cervix of uterus, and method for detecting abnormal cell in cervix of uterus by using same |
| CN108061677B (zh) * | 2010-03-12 | 2021-10-29 | 国立研究开发法人理化学研究所 | 用于生物材料的澄清试剂和其用途 |
| EP2711682B1 (en) | 2011-05-20 | 2019-07-10 | Riken | Clarifying reagent for biological materials and use thereof |
| JP6433901B2 (ja) | 2013-08-14 | 2018-12-05 | 国立研究開発法人理化学研究所 | 光透過性に優れた生物材料を調製するための組成物およびその利用 |
| CN111929122B (zh) * | 2020-06-29 | 2022-10-28 | 广州江元医疗科技有限公司 | 一种用于免疫细胞化学染色的抗原修复方法、细胞悬液及应用该方法的化学染色方法 |
| CN116298253A (zh) * | 2023-03-30 | 2023-06-23 | 河北明志康华生物科技有限公司 | 一种膀胱癌标记物核基质蛋白Nmp22的检测 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5424000A (en) * | 1993-10-05 | 1995-06-13 | West Agro, Inc. | Acid cleanings and staining compositions |
| BR9814271A (pt) * | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
| US6544798B1 (en) * | 1999-02-26 | 2003-04-08 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
| US6592821B1 (en) * | 1999-05-17 | 2003-07-15 | Caliper Technologies Corp. | Focusing of microparticles in microfluidic systems |
| FR2807652A1 (fr) * | 2000-04-18 | 2001-10-19 | Oreal | Composition pour la teinture d'oxydation des fibres comprenant une 1-(4-aminophenyl)-pyrrolidine et un polymere cationique |
| JP3797418B2 (ja) * | 2001-05-29 | 2006-07-19 | 茂樹 並松 | 抗原賦活化法及びその抗原賦活剤 |
| US20040260157A1 (en) * | 2003-06-20 | 2004-12-23 | Montes Miguel A. | Method for automated screening of cervical/endocervical malignant and premalignant epithelial lesions using flow cytometry with HPV DNA fluorescent in-situ hybridization ( FISH) technology |
| US8632994B2 (en) * | 2003-12-01 | 2014-01-21 | Dako Denmark A/S | Methods and compositions for immuno-histochemical detection |
| SI1924252T1 (sl) * | 2005-07-28 | 2011-12-30 | Id Fish Technology Inc | Metoda za izboljšanje celične permeabilnosti za tuje partikle |
-
2006
- 2006-12-28 JP JP2006355952A patent/JP5241100B2/ja not_active Expired - Fee Related
-
2007
- 2007-12-21 EP EP07150313A patent/EP1975615B1/en not_active Not-in-force
- 2007-12-21 AT AT07150313T patent/ATE447182T1/de not_active IP Right Cessation
- 2007-12-21 DE DE602007002981T patent/DE602007002981D1/de active Active
- 2007-12-28 US US11/966,318 patent/US7871783B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US7871783B2 (en) | 2011-01-18 |
| JP2008161138A (ja) | 2008-07-17 |
| US20080160542A1 (en) | 2008-07-03 |
| EP1975615B1 (en) | 2009-10-28 |
| DE602007002981D1 (de) | 2009-12-10 |
| EP1975615A1 (en) | 2008-10-01 |
| ATE447182T1 (de) | 2009-11-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102597740B (zh) | 用于抗原提取过程的方法和装置 | |
| EP1975615B1 (en) | Method for detecting a cell as well as use of an agent for breaking a hydrogen bond | |
| Abu Abed et al. | Immunofluorescence labelling of human and murine neutrophil extracellular traps in paraffin-embedded tissue | |
| Miller et al. | Developmental Validation of the SPERM HY‐LITERTM Kit for the Identification of Human Spermatozoa in Forensic Samples | |
| JP6877564B2 (ja) | 有機着色微粒子、診断薬キット、及びインビトロ診断方法 | |
| Jackson et al. | Quality assurance in immunochemistry | |
| CN102112875A (zh) | 宫颈部异常细胞检测用试剂及用该试剂检测宫颈部异常细胞的方法 | |
| US9176032B2 (en) | Methods of analyzing an H and E stained biological sample | |
| US7306926B2 (en) | Method for detecting carcinomas in a solubilized cervical body sample | |
| JP2020173204A (ja) | 画像処理システム、画像処理方法及びプログラム | |
| JP2603668B2 (ja) | 細胞スクリーニング、装置および方法 | |
| WO2018030547A1 (ja) | 血中循環癌細胞の検出方法及び血中循環癌細胞を検出するための前処理方法 | |
| WO2018029858A1 (ja) | 血中循環癌細胞の検出方法及び血中循環癌細胞を検出するための前処理方法 | |
| US20070054258A1 (en) | Method of removing mucus and cell treatment solution and preservative solution used therefor | |
| CN110073222A (zh) | 用于试剂递送的方法、系统和固体组合物 | |
| JPWO2018101327A1 (ja) | 検体中の特定のpsaの含有量に基づく、前立腺癌のグリーソンスコアの推定方法、病理病期分類の推定方法、および補助情報の取得方法 | |
| JPH1183849A (ja) | 尿中有形成分の分析方法及びその分析試薬 | |
| Takamura et al. | Development of a quantitative validation method for forensic investigation of human spermatozoa using a commercial fluorescence staining kit (SPERM HY-LITER™ Express) | |
| JP6583011B2 (ja) | 酸性水溶液を用いた免疫染色スライドの洗浄方法 | |
| Santangelo et al. | Special staining techniques: application and quality assurance | |
| CN110376105B (zh) | 一种用于确定临床样品中小颗粒特性和浓度的方法及装置 | |
| Collins et al. | Immunofluorescence Methods in the Diagnosis of Renal and Cardiac Diseases | |
| JPS62135769A (ja) | 細胞中の蛋白質とdnaの比率の測定法及び測定用試薬 | |
| CN111638349A (zh) | 一种检测胃癌患者外周血循环肿瘤细胞ca125表达的免疫荧光试剂盒及检测方法 | |
| JPWO2005038044A1 (ja) | 細胞処理方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20091127 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20091127 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20120612 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120810 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20130305 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130402 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20160412 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 Ref document number: 5241100 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |