JP2007500852A - 外部勾配クロマトフォーカシング - Google Patents

外部勾配クロマトフォーカシング Download PDF

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JP2007500852A
JP2007500852A JP2006533089A JP2006533089A JP2007500852A JP 2007500852 A JP2007500852 A JP 2007500852A JP 2006533089 A JP2006533089 A JP 2006533089A JP 2006533089 A JP2006533089 A JP 2006533089A JP 2007500852 A JP2007500852 A JP 2007500852A
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exchange adsorbent
temperature
initial
gradient
charged molecules
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JP2006533089A
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Japanese (ja)
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JP2007500852A5 (enExample
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ラッチェザー， アイ トソネフ，
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クリオバイオフィジカ， インコーポレイテッド
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Publication of JP2007500852A5 publication Critical patent/JP2007500852A5/ja
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B01D15/168—Fluid composition conditioning, e.g. gradient pH gradient or chromatofocusing, i.e. separation according to the isoelectric point pI
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Electrochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2006533089A 2003-05-16 2004-05-14 外部勾配クロマトフォーカシング Pending JP2007500852A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US47088903P 2003-05-16 2003-05-16
US49828703P 2003-08-28 2003-08-28
PCT/US2004/015216 WO2004103519A2 (en) 2003-05-16 2004-05-14 External gradient chromatofocusing

Publications (2)

Publication Number Publication Date
JP2007500852A true JP2007500852A (ja) 2007-01-18
JP2007500852A5 JP2007500852A5 (enExample) 2007-06-21

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Family Applications (1)

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JP2006533089A Pending JP2007500852A (ja) 2003-05-16 2004-05-14 外部勾配クロマトフォーカシング

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US (1) US7790025B2 (enExample)
EP (1) EP1628729A2 (enExample)
JP (1) JP2007500852A (enExample)
AU (1) AU2004241082A1 (enExample)
CA (1) CA2525880C (enExample)
WO (1) WO2004103519A2 (enExample)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012068244A (ja) * 2010-09-20 2012-04-05 Asahi Kasei Bioprocess Inc タンパク質の分離及び精製のための液体クロマトグラフィーのシステム及び方法
JP2013506127A (ja) * 2009-09-25 2013-02-21 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 分離システム及び方法
JP2013506128A (ja) * 2009-09-25 2013-02-21 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 液体混合物の調製方法及びシステム
JP2013529781A (ja) * 2010-06-23 2013-07-22 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 液体混合物の製造方法
JP2014048137A (ja) * 2012-08-30 2014-03-17 Shimadzu Corp 有機酸分析法
JP2016529489A (ja) * 2013-07-12 2016-09-23 ジェネンテック， インコーポレイテッド イオン交換クロマトグラフィーのインプットの最適化の解明
JP2017538129A (ja) * 2014-12-17 2017-12-21 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 溶液を混合するためのシステム
JP2018503809A (ja) * 2014-12-12 2018-02-08 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ クロマトグラフィ用の溶液を調製するためのシステム
JP2018513672A (ja) * 2015-02-25 2018-05-31 オムリックス・バイオファーマシューティカルズ・リミテッドＯｍｒｉｘ Ｂｉｏｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｌｔｄ． トロンビン及びその分解ポリペプチドの精製及び定量化方法
JP2020127435A (ja) * 2015-02-25 2020-08-27 オムリックス・バイオファーマシューティカルズ・リミテッドＯｍｒｉｘ Ｂｉｏｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｌｔｄ． トロンビン及びその分解ポリペプチドの精製及び定量化方法
JP2022091322A (ja) * 2020-12-09 2022-06-21 株式会社島津製作所 液体クロマトグラフのｐＨ管理システムおよびプログラム

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0400197D0 (sv) * 2004-01-29 2004-01-29 Amersham Biosciences Ab Method and system for reducing total sample complexity
WO2005100379A1 (ja) * 2004-03-30 2005-10-27 Hiroshi Yanagisawa タンパク質の分離方法
JP5026266B2 (ja) * 2004-08-12 2012-09-12 リポクセン テクノロジーズ リミテッド 分別
US8105983B2 (en) * 2005-03-29 2012-01-31 The Regents Of The University Of California High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins
CA2616976C (en) * 2005-07-28 2012-08-28 Bio-Rad Laboratories, Inc. Separation of proteins based on isoelectric point using solid-phase buffers
US7425263B2 (en) * 2006-08-29 2008-09-16 Cryobiophysica Multi-component, simultaneous, independent multi-gradient system for liquid chromatography
CN102906104B (zh) * 2009-09-10 2015-04-22 朗萨生物制剂有限公司 用于多肽纯化的方法和系统
KR20120119979A (ko) * 2010-01-26 2012-11-01 올테크 어소시에이츠, 인크. 액체 크로마토그래피 시스템에서 구배를 최적화하는 방법
US8921113B2 (en) * 2012-12-21 2014-12-30 Dionex Corporation Buffer kit and method of generating a linear pH gradient
WO2019067637A1 (en) * 2017-09-26 2019-04-04 Waters Technologies Corporation HIGH-PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER FOR RETENTION OF ACID ANALYTES
CN109096363A (zh) * 2018-09-07 2018-12-28 苏州美极医疗科技有限公司 一种线性pH梯度缓冲液及其应用
US20220135619A1 (en) 2019-02-24 2022-05-05 Bristol-Myers Squibb Company Methods of isolating a protein
US11022585B2 (en) 2019-06-09 2021-06-01 Dionex Corporation Methods and systems for optimizing buffer conditions with liquid chromatography
CN110348090B (zh) * 2019-06-28 2021-05-04 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
WO2024261724A1 (en) * 2023-06-23 2024-12-26 Lupin Limited Ion-exchange chromatography for separation and analysis of charge variants

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516054A (ja) * 1997-09-09 2001-09-25 グラクソ グループ リミテッド 分析法およびその装置
JP2002508840A (ja) * 1997-06-04 2002-03-19 ブレイエ・ユニバージテイト・ブリュッセル 流体の分離方法及びそのための装置
JP2003096092A (ja) * 2001-07-06 2003-04-03 Alfa Wassermann Spa 陽イオン交換クロマトグラフィーを介する薬理学的に活性なタンパク質の精製方法

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SE440699B (sv) 1984-02-06 1985-08-12 Pharmacia Ab Sett att medelst isotransferrinbestemning uppskatta en individs alkoholkonsumtion
US7425263B2 (en) * 2006-08-29 2008-09-16 Cryobiophysica Multi-component, simultaneous, independent multi-gradient system for liquid chromatography

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002508840A (ja) * 1997-06-04 2002-03-19 ブレイエ・ユニバージテイト・ブリュッセル 流体の分離方法及びそのための装置
JP2001516054A (ja) * 1997-09-09 2001-09-25 グラクソ グループ リミテッド 分析法およびその装置
JP2003096092A (ja) * 2001-07-06 2003-04-03 Alfa Wassermann Spa 陽イオン交換クロマトグラフィーを介する薬理学的に活性なタンパク質の精製方法

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013506127A (ja) * 2009-09-25 2013-02-21 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 分離システム及び方法
JP2013506128A (ja) * 2009-09-25 2013-02-21 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 液体混合物の調製方法及びシステム
JP2013529781A (ja) * 2010-06-23 2013-07-22 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 液体混合物の製造方法
JP2012068244A (ja) * 2010-09-20 2012-04-05 Asahi Kasei Bioprocess Inc タンパク質の分離及び精製のための液体クロマトグラフィーのシステム及び方法
JP2014048137A (ja) * 2012-08-30 2014-03-17 Shimadzu Corp 有機酸分析法
US10274466B2 (en) 2013-07-12 2019-04-30 Genentech, Inc. Elucidation of ion exchange chromatography input optimization
JP2016529489A (ja) * 2013-07-12 2016-09-23 ジェネンテック， インコーポレイテッド イオン交換クロマトグラフィーのインプットの最適化の解明
US10921297B2 (en) 2013-07-12 2021-02-16 Genentech, Inc. Elucidation of ion exchange chromatography input optimization
JP2018503809A (ja) * 2014-12-12 2018-02-08 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ クロマトグラフィ用の溶液を調製するためのシステム
JP2017538129A (ja) * 2014-12-17 2017-12-21 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 溶液を混合するためのシステム
JP2018513672A (ja) * 2015-02-25 2018-05-31 オムリックス・バイオファーマシューティカルズ・リミテッドＯｍｒｉｘ Ｂｉｏｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｌｔｄ． トロンビン及びその分解ポリペプチドの精製及び定量化方法
JP2020127435A (ja) * 2015-02-25 2020-08-27 オムリックス・バイオファーマシューティカルズ・リミテッドＯｍｒｉｘ Ｂｉｏｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｌｔｄ． トロンビン及びその分解ポリペプチドの精製及び定量化方法
JP2022091322A (ja) * 2020-12-09 2022-06-21 株式会社島津製作所 液体クロマトグラフのｐＨ管理システムおよびプログラム
US12474312B2 (en) 2020-12-09 2025-11-18 Shimadzu Corporation PH management system of liquid chromatograph and computer readable recording medium recording program

Also Published As

Publication number Publication date
US7790025B2 (en) 2010-09-07
CA2525880C (en) 2011-10-11
WO2004103519A3 (en) 2005-02-03
EP1628729A2 (en) 2006-03-01
AU2004241082A2 (en) 2004-12-02
WO2004103519A2 (en) 2004-12-02
US20070144973A1 (en) 2007-06-28
AU2004241082A1 (en) 2004-12-02
CA2525880A1 (en) 2004-12-02

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