JP2007500852A5 - - Google Patents
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- Publication number
- JP2007500852A5 JP2007500852A5 JP2006533089A JP2006533089A JP2007500852A5 JP 2007500852 A5 JP2007500852 A5 JP 2007500852A5 JP 2006533089 A JP2006533089 A JP 2006533089A JP 2006533089 A JP2006533089 A JP 2006533089A JP 2007500852 A5 JP2007500852 A5 JP 2007500852A5
- Authority
- JP
- Japan
- Prior art keywords
- exchange adsorbent
- temperature
- initial
- gradient
- cation exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003463 adsorbent Substances 0.000 claims 263
- 239000000872 buffer Substances 0.000 claims 118
- 238000005349 anion exchange Methods 0.000 claims 112
- 238000005341 cation exchange Methods 0.000 claims 111
- 238000000034 method Methods 0.000 claims 88
- 239000000243 solution Substances 0.000 claims 77
- 239000003480 eluent Substances 0.000 claims 37
- 239000002904 solvent Substances 0.000 claims 35
- 230000036962 time dependent Effects 0.000 claims 28
- 238000004587 chromatography analysis Methods 0.000 claims 24
- 238000005342 ion exchange Methods 0.000 claims 23
- 238000011098 chromatofocusing Methods 0.000 claims 22
- 239000000203 mixture Substances 0.000 claims 16
- 150000001875 compounds Chemical class 0.000 claims 14
- 238000010828 elution Methods 0.000 claims 12
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 claims 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 8
- 238000005086 pumping Methods 0.000 claims 8
- 230000007704 transition Effects 0.000 claims 8
- 230000007423 decrease Effects 0.000 claims 6
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims 6
- 230000000717 retained effect Effects 0.000 claims 6
- 239000012609 strong anion exchange resin Substances 0.000 claims 6
- 239000012607 strong cation exchange resin Substances 0.000 claims 6
- PVOAHINGSUIXLS-UHFFFAOYSA-N 1-Methylpiperazine Chemical compound CN1CCNCC1 PVOAHINGSUIXLS-UHFFFAOYSA-N 0.000 claims 5
- 239000007989 BIS-Tris Propane buffer Substances 0.000 claims 5
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims 5
- HHKZCCWKTZRCCL-UHFFFAOYSA-N bis-tris propane Chemical compound OCC(CO)(CO)NCCCNC(CO)(CO)CO HHKZCCWKTZRCCL-UHFFFAOYSA-N 0.000 claims 5
- 238000000926 separation method Methods 0.000 claims 5
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 claims 4
- ABLZXFCXXLZCGV-UHFFFAOYSA-N Phosphorous acid Chemical compound OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 claims 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 4
- 150000001450 anions Chemical class 0.000 claims 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 4
- 230000001419 dependent effect Effects 0.000 claims 4
- 125000000524 functional group Chemical group 0.000 claims 4
- 150000003949 imides Chemical class 0.000 claims 4
- 125000001841 imino group Chemical group [H]N=* 0.000 claims 4
- 230000007935 neutral effect Effects 0.000 claims 4
- 239000006174 pH buffer Substances 0.000 claims 4
- 230000001105 regulatory effect Effects 0.000 claims 4
- 150000003457 sulfones Chemical class 0.000 claims 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims 3
- 150000001768 cations Chemical class 0.000 claims 3
- 235000019253 formic acid Nutrition 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 3
- 239000012610 weak anion exchange resin Substances 0.000 claims 3
- 239000012608 weak cation exchange resin Substances 0.000 claims 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 238000001816 cooling Methods 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 238000005470 impregnation Methods 0.000 claims 2
- 150000002500 ions Chemical class 0.000 claims 2
- 238000001179 sorption measurement Methods 0.000 claims 2
- 239000003957 anion exchange resin Substances 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 239000003729 cation exchange resin Substances 0.000 claims 1
- 238000013375 chromatographic separation Methods 0.000 claims 1
- 239000003456 ion exchange resin Substances 0.000 claims 1
- 229920003303 ion-exchange polymer Polymers 0.000 claims 1
- 239000011259 mixed solution Substances 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 238000001139 pH measurement Methods 0.000 claims 1
- 239000002594 sorbent Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US47088903P | 2003-05-16 | 2003-05-16 | |
| US49828703P | 2003-08-28 | 2003-08-28 | |
| PCT/US2004/015216 WO2004103519A2 (en) | 2003-05-16 | 2004-05-14 | External gradient chromatofocusing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2007500852A JP2007500852A (ja) | 2007-01-18 |
| JP2007500852A5 true JP2007500852A5 (enExample) | 2007-06-21 |
Family
ID=33479267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006533089A Pending JP2007500852A (ja) | 2003-05-16 | 2004-05-14 | 外部勾配クロマトフォーカシング |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7790025B2 (enExample) |
| EP (1) | EP1628729A2 (enExample) |
| JP (1) | JP2007500852A (enExample) |
| AU (1) | AU2004241082A1 (enExample) |
| CA (1) | CA2525880C (enExample) |
| WO (1) | WO2004103519A2 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE0400197D0 (sv) * | 2004-01-29 | 2004-01-29 | Amersham Biosciences Ab | Method and system for reducing total sample complexity |
| WO2005100379A1 (ja) * | 2004-03-30 | 2005-10-27 | Hiroshi Yanagisawa | タンパク質の分離方法 |
| JP5026266B2 (ja) * | 2004-08-12 | 2012-09-12 | リポクセン テクノロジーズ リミテッド | 分別 |
| US8105983B2 (en) * | 2005-03-29 | 2012-01-31 | The Regents Of The University Of California | High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins |
| CA2616976C (en) * | 2005-07-28 | 2012-08-28 | Bio-Rad Laboratories, Inc. | Separation of proteins based on isoelectric point using solid-phase buffers |
| US7425263B2 (en) * | 2006-08-29 | 2008-09-16 | Cryobiophysica | Multi-component, simultaneous, independent multi-gradient system for liquid chromatography |
| CN102906104B (zh) * | 2009-09-10 | 2015-04-22 | 朗萨生物制剂有限公司 | 用于多肽纯化的方法和系统 |
| CN107983261A (zh) | 2009-09-25 | 2018-05-04 | 通用电气健康护理生物科学股份公司 | 用于液体混合物的制备的方法和系统 |
| US9527010B2 (en) * | 2009-09-25 | 2016-12-27 | Ge Healthcare Bio-Sciences Corp. | Separation system and method |
| KR20120119979A (ko) * | 2010-01-26 | 2012-11-01 | 올테크 어소시에이츠, 인크. | 액체 크로마토그래피 시스템에서 구배를 최적화하는 방법 |
| CN102947768B (zh) * | 2010-06-23 | 2016-05-18 | 通用电气健康护理生物科学股份公司 | 制备液体混合物的方法 |
| US20130109102A1 (en) * | 2010-09-20 | 2013-05-02 | Hiroyuki Yabe | Liquid chromatography system and method for protein separation and purification |
| JP5935603B2 (ja) * | 2012-08-30 | 2016-06-15 | 株式会社島津製作所 | 有機酸分析法 |
| US8921113B2 (en) * | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
| ES2705700T3 (es) * | 2013-07-12 | 2019-03-26 | Hoffmann La Roche | Elucidación de la optimización de entrada en cromatografía de intercambio iónico |
| EP3230729B1 (en) | 2014-12-12 | 2024-03-06 | Cytiva Sweden AB | System for preparing solutions for chromatography |
| WO2016097131A1 (en) * | 2014-12-17 | 2016-06-23 | Ge Healthcare Bio-Sciences Ab | System for blending solutions |
| ES2791982T3 (es) * | 2015-02-25 | 2020-11-06 | Omrix Biopharmaceuticals Ltd | Método para purificar y cuantificar la trombina y sus polipéptidos de degradación |
| EP3262409B1 (en) * | 2015-02-25 | 2020-03-25 | Omrix Biopharmaceuticals Ltd. | Method for purifying and quantifying thrombin and its degradation polypeptides |
| WO2019067637A1 (en) * | 2017-09-26 | 2019-04-04 | Waters Technologies Corporation | HIGH-PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER FOR RETENTION OF ACID ANALYTES |
| CN109096363A (zh) * | 2018-09-07 | 2018-12-28 | 苏州美极医疗科技有限公司 | 一种线性pH梯度缓冲液及其应用 |
| US20220135619A1 (en) | 2019-02-24 | 2022-05-05 | Bristol-Myers Squibb Company | Methods of isolating a protein |
| US11022585B2 (en) | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
| CN110348090B (zh) * | 2019-06-28 | 2021-05-04 | 浙江大学 | 基于人工神经网络实现多柱连续流层析设计及分析的方法 |
| JP2022091322A (ja) | 2020-12-09 | 2022-06-21 | 株式会社島津製作所 | 液体クロマトグラフのｐＨ管理システムおよびプログラム |
| WO2024261724A1 (en) * | 2023-06-23 | 2024-12-26 | Lupin Limited | Ion-exchange chromatography for separation and analysis of charge variants |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE440699B (sv) | 1984-02-06 | 1985-08-12 | Pharmacia Ab | Sett att medelst isotransferrinbestemning uppskatta en individs alkoholkonsumtion |
| EP0882982A1 (en) * | 1997-06-04 | 1998-12-09 | Vrije Universiteit Brussel | Method for separating a fluid substance and device therefor |
| GB9719142D0 (en) * | 1997-09-09 | 1997-11-12 | Glaxo Group Ltd | Analytical method and apparatus therefor |
| ITBO20010426A1 (it) * | 2001-07-06 | 2003-01-06 | Alfa Wassermann Spa | Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico |
| US7425263B2 (en) * | 2006-08-29 | 2008-09-16 | Cryobiophysica | Multi-component, simultaneous, independent multi-gradient system for liquid chromatography |
-
2004
- 2004-05-14 WO PCT/US2004/015216 patent/WO2004103519A2/en not_active Ceased
- 2004-05-14 AU AU2004241082A patent/AU2004241082A1/en not_active Abandoned
- 2004-05-14 JP JP2006533089A patent/JP2007500852A/ja active Pending
- 2004-05-14 US US10/556,562 patent/US7790025B2/en active Active - Reinstated
- 2004-05-14 EP EP04752276A patent/EP1628729A2/en not_active Withdrawn
- 2004-05-14 CA CA2525880A patent/CA2525880C/en not_active Expired - Lifetime
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