CA2525880C - External gradient chromatofocusing - Google Patents
External gradient chromatofocusing Download PDFInfo
- Publication number
- CA2525880C CA2525880C CA2525880A CA2525880A CA2525880C CA 2525880 C CA2525880 C CA 2525880C CA 2525880 A CA2525880 A CA 2525880A CA 2525880 A CA2525880 A CA 2525880A CA 2525880 C CA2525880 C CA 2525880C
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- CA
- Canada
- Prior art keywords
- gradient
- exchange adsorbent
- charged molecules
- temperature
- initial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- HTOOKGDPMXSJSY-UHFFFAOYSA-N histidinyl-tyrosine Chemical compound C=1C=C(O)C=CC=1CC(C(O)=O)NC(=O)C(N)CC1=CN=CN1 HTOOKGDPMXSJSY-UHFFFAOYSA-N 0.000 description 1
- QDYTUZCWBJRHKK-UHFFFAOYSA-N imidazole-4-methanol Chemical compound OCC1=CNC=N1 QDYTUZCWBJRHKK-UHFFFAOYSA-N 0.000 description 1
- 239000012729 immediate-release (IR) formulation Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- CRVGTESFCCXCTH-UHFFFAOYSA-N methyl diethanolamine Chemical compound OCCN(C)CCO CRVGTESFCCXCTH-UHFFFAOYSA-N 0.000 description 1
- ZIYVHBGGAOATLY-UHFFFAOYSA-N methylmalonic acid Chemical compound OC(=O)C(C)C(O)=O ZIYVHBGGAOATLY-UHFFFAOYSA-N 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- UDGSVBYJWHOHNN-UHFFFAOYSA-N n',n'-diethylethane-1,2-diamine Chemical compound CCN(CC)CCN UDGSVBYJWHOHNN-UHFFFAOYSA-N 0.000 description 1
- DILRJUIACXKSQE-UHFFFAOYSA-N n',n'-dimethylethane-1,2-diamine Chemical compound CN(C)CCN DILRJUIACXKSQE-UHFFFAOYSA-N 0.000 description 1
- DFPGBRPWDZFIPP-UHFFFAOYSA-N n'-butylethane-1,2-diamine Chemical compound CCCCNCCN DFPGBRPWDZFIPP-UHFFFAOYSA-N 0.000 description 1
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- KFIGICHILYTCJF-UHFFFAOYSA-N n'-methylethane-1,2-diamine Chemical compound CNCCN KFIGICHILYTCJF-UHFFFAOYSA-N 0.000 description 1
- CFNHVUGPXZUTRR-UHFFFAOYSA-N n'-propylethane-1,2-diamine Chemical compound CCCNCCN CFNHVUGPXZUTRR-UHFFFAOYSA-N 0.000 description 1
- KVKFRMCSXWQSNT-UHFFFAOYSA-N n,n'-dimethylethane-1,2-diamine Chemical compound CNCCNC KVKFRMCSXWQSNT-UHFFFAOYSA-N 0.000 description 1
- VATUKUMHBXZSCD-UHFFFAOYSA-N n,n'-dipropylethane-1,2-diamine Chemical compound CCCNCCNCCC VATUKUMHBXZSCD-UHFFFAOYSA-N 0.000 description 1
- VMESOKCXSYNAKD-UHFFFAOYSA-N n,n-dimethylhydroxylamine Chemical compound CN(C)O VMESOKCXSYNAKD-UHFFFAOYSA-N 0.000 description 1
- RWIVICVCHVMHMU-UHFFFAOYSA-N n-aminoethylmorpholine Chemical compound NCCN1CCOCC1 RWIVICVCHVMHMU-UHFFFAOYSA-N 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- WPBWJEYRHXACLR-UHFFFAOYSA-N nonanedioic acid Chemical compound OC(=O)CCCCCCCC(O)=O.OC(=O)CCCCCCCC(O)=O WPBWJEYRHXACLR-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229960002429 proline Drugs 0.000 description 1
- PZZICILSCNDOKK-UHFFFAOYSA-N propane-1,2,3-triamine Chemical compound NCC(N)CN PZZICILSCNDOKK-UHFFFAOYSA-N 0.000 description 1
- AOHJOMMDDJHIJH-UHFFFAOYSA-N propylenediamine Chemical compound CC(N)CN AOHJOMMDDJHIJH-UHFFFAOYSA-N 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 125000001453 quaternary ammonium group Chemical group 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- YCLWMUYXEGEIGD-UHFFFAOYSA-M sodium;2-hydroxy-3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonate Chemical compound [Na+].OCCN1CCN(CC(O)CS([O-])(=O)=O)CC1 YCLWMUYXEGEIGD-UHFFFAOYSA-M 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000012609 strong anion exchange resin Substances 0.000 description 1
- 238000012799 strong cation exchange Methods 0.000 description 1
- 239000012607 strong cation exchange resin Substances 0.000 description 1
- 229950000244 sulfanilic acid Drugs 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000012784 weak cation exchange Methods 0.000 description 1
- 239000012608 weak cation exchange resin Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
- B01D15/168—Fluid composition conditioning, e.g. gradient pH gradient or chromatofocusing, i.e. separation according to the isoelectric point pI
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
- C07K1/28—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Electrochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US47088903P | 2003-05-16 | 2003-05-16 | |
| US60/470,889 | 2003-05-16 | ||
| US49828703P | 2003-08-28 | 2003-08-28 | |
| US60/498,287 | 2003-08-28 | ||
| PCT/US2004/015216 WO2004103519A2 (en) | 2003-05-16 | 2004-05-14 | External gradient chromatofocusing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2525880A1 CA2525880A1 (en) | 2004-12-02 |
| CA2525880C true CA2525880C (en) | 2011-10-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2525880A Expired - Lifetime CA2525880C (en) | 2003-05-16 | 2004-05-14 | External gradient chromatofocusing |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7790025B2 (enExample) |
| EP (1) | EP1628729A2 (enExample) |
| JP (1) | JP2007500852A (enExample) |
| AU (1) | AU2004241082A1 (enExample) |
| CA (1) | CA2525880C (enExample) |
| WO (1) | WO2004103519A2 (enExample) |
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| SE0400197D0 (sv) * | 2004-01-29 | 2004-01-29 | Amersham Biosciences Ab | Method and system for reducing total sample complexity |
| WO2005100379A1 (ja) * | 2004-03-30 | 2005-10-27 | Hiroshi Yanagisawa | タンパク質の分離方法 |
| JP5026266B2 (ja) * | 2004-08-12 | 2012-09-12 | リポクセン テクノロジーズ リミテッド | 分別 |
| US8105983B2 (en) * | 2005-03-29 | 2012-01-31 | The Regents Of The University Of California | High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins |
| CA2616976C (en) * | 2005-07-28 | 2012-08-28 | Bio-Rad Laboratories, Inc. | Separation of proteins based on isoelectric point using solid-phase buffers |
| US7425263B2 (en) * | 2006-08-29 | 2008-09-16 | Cryobiophysica | Multi-component, simultaneous, independent multi-gradient system for liquid chromatography |
| CN102906104B (zh) * | 2009-09-10 | 2015-04-22 | 朗萨生物制剂有限公司 | 用于多肽纯化的方法和系统 |
| CN107983261A (zh) | 2009-09-25 | 2018-05-04 | 通用电气健康护理生物科学股份公司 | 用于液体混合物的制备的方法和系统 |
| US9527010B2 (en) * | 2009-09-25 | 2016-12-27 | Ge Healthcare Bio-Sciences Corp. | Separation system and method |
| KR20120119979A (ko) * | 2010-01-26 | 2012-11-01 | 올테크 어소시에이츠, 인크. | 액체 크로마토그래피 시스템에서 구배를 최적화하는 방법 |
| CN102947768B (zh) * | 2010-06-23 | 2016-05-18 | 通用电气健康护理生物科学股份公司 | 制备液体混合物的方法 |
| US20130109102A1 (en) * | 2010-09-20 | 2013-05-02 | Hiroyuki Yabe | Liquid chromatography system and method for protein separation and purification |
| JP5935603B2 (ja) * | 2012-08-30 | 2016-06-15 | 株式会社島津製作所 | 有機酸分析法 |
| US8921113B2 (en) * | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
| ES2705700T3 (es) * | 2013-07-12 | 2019-03-26 | Hoffmann La Roche | Elucidación de la optimización de entrada en cromatografía de intercambio iónico |
| EP3230729B1 (en) | 2014-12-12 | 2024-03-06 | Cytiva Sweden AB | System for preparing solutions for chromatography |
| WO2016097131A1 (en) * | 2014-12-17 | 2016-06-23 | Ge Healthcare Bio-Sciences Ab | System for blending solutions |
| ES2791982T3 (es) * | 2015-02-25 | 2020-11-06 | Omrix Biopharmaceuticals Ltd | Método para purificar y cuantificar la trombina y sus polipéptidos de degradación |
| EP3262409B1 (en) * | 2015-02-25 | 2020-03-25 | Omrix Biopharmaceuticals Ltd. | Method for purifying and quantifying thrombin and its degradation polypeptides |
| WO2019067637A1 (en) * | 2017-09-26 | 2019-04-04 | Waters Technologies Corporation | HIGH-PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER FOR RETENTION OF ACID ANALYTES |
| CN109096363A (zh) * | 2018-09-07 | 2018-12-28 | 苏州美极医疗科技有限公司 | 一种线性pH梯度缓冲液及其应用 |
| US20220135619A1 (en) | 2019-02-24 | 2022-05-05 | Bristol-Myers Squibb Company | Methods of isolating a protein |
| US11022585B2 (en) | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
| CN110348090B (zh) * | 2019-06-28 | 2021-05-04 | 浙江大学 | 基于人工神经网络实现多柱连续流层析设计及分析的方法 |
| JP2022091322A (ja) | 2020-12-09 | 2022-06-21 | 株式会社島津製作所 | 液体クロマトグラフのｐＨ管理システムおよびプログラム |
| WO2024261724A1 (en) * | 2023-06-23 | 2024-12-26 | Lupin Limited | Ion-exchange chromatography for separation and analysis of charge variants |
Family Cites Families (5)
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| SE440699B (sv) | 1984-02-06 | 1985-08-12 | Pharmacia Ab | Sett att medelst isotransferrinbestemning uppskatta en individs alkoholkonsumtion |
| EP0882982A1 (en) * | 1997-06-04 | 1998-12-09 | Vrije Universiteit Brussel | Method for separating a fluid substance and device therefor |
| GB9719142D0 (en) * | 1997-09-09 | 1997-11-12 | Glaxo Group Ltd | Analytical method and apparatus therefor |
| ITBO20010426A1 (it) * | 2001-07-06 | 2003-01-06 | Alfa Wassermann Spa | Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico |
| US7425263B2 (en) * | 2006-08-29 | 2008-09-16 | Cryobiophysica | Multi-component, simultaneous, independent multi-gradient system for liquid chromatography |
-
2004
- 2004-05-14 WO PCT/US2004/015216 patent/WO2004103519A2/en not_active Ceased
- 2004-05-14 AU AU2004241082A patent/AU2004241082A1/en not_active Abandoned
- 2004-05-14 JP JP2006533089A patent/JP2007500852A/ja active Pending
- 2004-05-14 US US10/556,562 patent/US7790025B2/en active Active - Reinstated
- 2004-05-14 EP EP04752276A patent/EP1628729A2/en not_active Withdrawn
- 2004-05-14 CA CA2525880A patent/CA2525880C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7790025B2 (en) | 2010-09-07 |
| WO2004103519A3 (en) | 2005-02-03 |
| EP1628729A2 (en) | 2006-03-01 |
| AU2004241082A2 (en) | 2004-12-02 |
| WO2004103519A2 (en) | 2004-12-02 |
| US20070144973A1 (en) | 2007-06-28 |
| AU2004241082A1 (en) | 2004-12-02 |
| CA2525880A1 (en) | 2004-12-02 |
| JP2007500852A (ja) | 2007-01-18 |
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