JP2006523463A5 - - Google Patents
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- Publication number
- JP2006523463A5 JP2006523463A5 JP2006513124A JP2006513124A JP2006523463A5 JP 2006523463 A5 JP2006523463 A5 JP 2006523463A5 JP 2006513124 A JP2006513124 A JP 2006513124A JP 2006513124 A JP2006513124 A JP 2006513124A JP 2006523463 A5 JP2006523463 A5 JP 2006523463A5
- Authority
- JP
- Japan
- Prior art keywords
- rna
- solid support
- solution
- biological material
- lithium salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007787 solid Substances 0.000 claims 16
- 238000000034 method Methods 0.000 claims 15
- 239000012620 biological material Substances 0.000 claims 10
- 229910003002 lithium salt Inorganic materials 0.000 claims 10
- 159000000002 lithium salts Chemical class 0.000 claims 10
- 238000005406 washing Methods 0.000 claims 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 8
- 230000009089 cytolysis Effects 0.000 claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- 102000039446 nucleic acids Human genes 0.000 claims 7
- 108020004707 nucleic acids Proteins 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 4
- 238000009472 formulation Methods 0.000 claims 4
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims 4
- 239000000377 silicon dioxide Substances 0.000 claims 4
- 239000000872 buffer Substances 0.000 claims 3
- 239000002738 chelating agent Substances 0.000 claims 3
- 238000010828 elution Methods 0.000 claims 3
- 239000006166 lysate Substances 0.000 claims 3
- 238000010532 solid phase synthesis reaction Methods 0.000 claims 3
- 239000012535 impurity Substances 0.000 claims 2
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims 2
- 239000012472 biological sample Substances 0.000 claims 1
- 239000006172 buffering agent Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000003638 chemical reducing agent Substances 0.000 claims 1
- 239000000344 soap Substances 0.000 claims 1
- 239000004094 surface-active agent Substances 0.000 claims 1
- PBYZMCDFOULPGH-UHFFFAOYSA-N tungstate Chemical compound [O-][W]([O-])(=O)=O PBYZMCDFOULPGH-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/418,194 | 2003-04-16 | ||
| US10/418,194 US7148343B2 (en) | 2001-10-12 | 2003-04-16 | Compositions and methods for using a solid support to purify RNA |
| PCT/US2004/012033 WO2004094635A2 (en) | 2003-04-16 | 2004-04-15 | Compositions and methods for using a solid support to purify rna |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010181955A Division JP2010263919A (ja) | 2003-04-16 | 2010-08-16 | 固体支持体を使用してrnaを精製するための、組成物および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006523463A JP2006523463A (ja) | 2006-10-19 |
| JP2006523463A5 true JP2006523463A5 (enExample) | 2007-06-07 |
| JP4804347B2 JP4804347B2 (ja) | 2011-11-02 |
Family
ID=33309522
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006513124A Expired - Lifetime JP4804347B2 (ja) | 2003-04-16 | 2004-04-15 | 固体支持体を使用してrnaを精製するための、組成物および方法 |
| JP2010181955A Pending JP2010263919A (ja) | 2003-04-16 | 2010-08-16 | 固体支持体を使用してrnaを精製するための、組成物および方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010181955A Pending JP2010263919A (ja) | 2003-04-16 | 2010-08-16 | 固体支持体を使用してrnaを精製するための、組成物および方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US7148343B2 (enExample) |
| EP (1) | EP1618194B1 (enExample) |
| JP (2) | JP4804347B2 (enExample) |
| CA (1) | CA2522446C (enExample) |
| WO (1) | WO2004094635A2 (enExample) |
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- 2004-04-15 JP JP2006513124A patent/JP4804347B2/ja not_active Expired - Lifetime
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- 2004-04-15 CA CA2522446A patent/CA2522446C/en not_active Expired - Lifetime
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