JP2005505305A5 - - Google Patents
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- Publication number
- JP2005505305A5 JP2005505305A5 JP2003536461A JP2003536461A JP2005505305A5 JP 2005505305 A5 JP2005505305 A5 JP 2005505305A5 JP 2003536461 A JP2003536461 A JP 2003536461A JP 2003536461 A JP2003536461 A JP 2003536461A JP 2005505305 A5 JP2005505305 A5 JP 2005505305A5
- Authority
- JP
- Japan
- Prior art keywords
- rna
- biological material
- solid support
- salt
- undegraded
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 23
- 238000000034 method Methods 0.000 claims 16
- 239000012620 biological material Substances 0.000 claims 15
- 239000007787 solid Substances 0.000 claims 12
- 238000010828 elution Methods 0.000 claims 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 7
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- 102000039446 nucleic acids Human genes 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 6
- 230000004570 RNA-binding Effects 0.000 claims 5
- -1 eluate Substances 0.000 claims 5
- 239000000377 silicon dioxide Substances 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 3
- 229910052783 alkali metal Inorganic materials 0.000 claims 2
- 239000008280 blood Substances 0.000 claims 2
- 210000004369 blood Anatomy 0.000 claims 2
- 230000003196 chaotropic effect Effects 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 229920000728 polyester Polymers 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 239000004094 surface-active agent Substances 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- FCKYPQBAHLOOJQ-UHFFFAOYSA-N Cyclohexane-1,2-diaminetetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)C1CCCCC1N(CC(O)=O)CC(O)=O FCKYPQBAHLOOJQ-UHFFFAOYSA-N 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims 1
- 241000204031 Mycoplasma Species 0.000 claims 1
- 239000000020 Nitrocellulose Substances 0.000 claims 1
- 239000004677 Nylon Substances 0.000 claims 1
- 239000002033 PVDF binder Substances 0.000 claims 1
- 239000004695 Polyether sulfone Substances 0.000 claims 1
- 241000606701 Rickettsia Species 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 108020004566 Transfer RNA Proteins 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 108020000999 Viral RNA Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000027455 binding Effects 0.000 claims 1
- 210000001185 bone marrow Anatomy 0.000 claims 1
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical class [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 229920002301 cellulose acetate Polymers 0.000 claims 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 210000003608 fece Anatomy 0.000 claims 1
- 229910003002 lithium salt Inorganic materials 0.000 claims 1
- 159000000002 lithium salts Chemical class 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 229920001220 nitrocellulos Polymers 0.000 claims 1
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- 229920001778 nylon Polymers 0.000 claims 1
- 229920006393 polyether sulfone Polymers 0.000 claims 1
- 229920000098 polyolefin Polymers 0.000 claims 1
- 229920000136 polysorbate Polymers 0.000 claims 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 210000001236 prokaryotic cell Anatomy 0.000 claims 1
- 108020004418 ribosomal RNA Proteins 0.000 claims 1
- 150000003297 rubidium Chemical class 0.000 claims 1
- 210000003296 saliva Anatomy 0.000 claims 1
- 239000013049 sediment Substances 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 159000000000 sodium salts Chemical class 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- 210000004243 sweat Anatomy 0.000 claims 1
- 210000001138 tear Anatomy 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2001/032073 WO2003033739A1 (en) | 2001-10-12 | 2001-10-12 | Compositions and methods for using a solid support to purify rna |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005505305A JP2005505305A (ja) | 2005-02-24 |
| JP2005505305A5 true JP2005505305A5 (enExample) | 2006-01-05 |
| JP3979996B2 JP3979996B2 (ja) | 2007-09-19 |
Family
ID=21742909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003536461A Expired - Lifetime JP3979996B2 (ja) | 2001-10-12 | 2001-10-12 | 固体支持体を使用してｒｎａを精製するための組成物および方法 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1438426A1 (enExample) |
| JP (1) | JP3979996B2 (enExample) |
| AU (1) | AU2002211719B2 (enExample) |
| CA (1) | CA2463317A1 (enExample) |
| WO (1) | WO2003033739A1 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001516731A (ja) | 1997-09-17 | 2001-10-02 | ジエントラ・システムズ・インコーポレーテツド | 核酸を単離するための装置および方法 |
| US7148343B2 (en) | 2001-10-12 | 2006-12-12 | Gentra Systems, Inc. | Compositions and methods for using a solid support to purify RNA |
| US20050032105A1 (en) | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| DK1442045T3 (da) * | 2001-11-06 | 2009-02-09 | Cortex Biochem Inc | Isolering og oprensning af nukleinsyrer |
| AU2002359522A1 (en) | 2001-11-28 | 2003-06-10 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| JP3983125B2 (ja) * | 2002-07-19 | 2007-09-26 | 富士フイルム株式会社 | 核酸の分離精製方法 |
| US20050009036A1 (en) * | 2003-07-11 | 2005-01-13 | Applera Corporation | Methods and kits for obtaining nucleic acid from biological samples |
| JP4435787B2 (ja) | 2003-12-16 | 2010-03-24 | キアジェン ノース アメリカン ホールディングス，インコーポレイティド | タンパク質を変性させるための処方物および方法 |
| WO2005093065A1 (en) * | 2004-03-16 | 2005-10-06 | Roche Diagnostics Gmbh | Improved method of isolating nucleic acids |
| EP1809744B1 (en) * | 2004-11-05 | 2018-06-06 | QIAGEN North American Holdings, Inc. | Compositions and methods for purifying nucleic acids from stabilization reagents |
| JP4956727B2 (ja) * | 2005-08-30 | 2012-06-20 | 倉敷紡績株式会社 | Ｒｎａ分離精製方法 |
| US20070092403A1 (en) * | 2005-10-21 | 2007-04-26 | Alan Wirbisky | Compact apparatus, compositions and methods for purifying nucleic acids |
| GB0719022D0 (en) * | 2007-09-28 | 2007-11-07 | Mole Genetics As | Isolation method |
| MX347611B (es) | 2011-06-19 | 2017-05-04 | Abogen Inc | Dispositivos, soluciones y metodos para recoleccion de muestras. |
| ES2981185T3 (es) * | 2013-03-14 | 2024-10-07 | Translate Bio Inc | Métodos para la purificación de ARN mensajero |
| RU2717644C2 (ru) | 2014-03-07 | 2020-03-24 | ДиЭнЭй ГЕНОТЕК ИНК. | Способ, композиция и набор для стабилизации дезоксирибонуклеиновых кислот в биологических образцах |
| MX373952B (es) | 2014-04-25 | 2020-07-13 | Shire Human Genetic Therapies | Métodos de purificación de arn mensajero. |
| WO2020041793A1 (en) | 2018-08-24 | 2020-02-27 | Translate Bio, Inc. | Methods for purification of messenger rna |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5010183A (en) * | 1989-07-07 | 1991-04-23 | Macfarlane Donald E | Process for purifying DNA and RNA using cationic detergents |
| AU6230594A (en) * | 1993-02-01 | 1994-08-29 | University Of Iowa Research Foundation, The | Quartenary amine surfactants and methods of using same in isolation of rna |
| ATE184013T1 (de) * | 1994-06-14 | 1999-09-15 | Invitek Gmbh | Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien |
| US5939259A (en) * | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
-
2001
- 2001-10-12 AU AU2002211719A patent/AU2002211719B2/en not_active Expired
- 2001-10-12 JP JP2003536461A patent/JP3979996B2/ja not_active Expired - Lifetime
- 2001-10-12 EP EP01979794A patent/EP1438426A1/en not_active Withdrawn
- 2001-10-12 CA CA002463317A patent/CA2463317A1/en not_active Abandoned
- 2001-10-12 WO PCT/US2001/032073 patent/WO2003033739A1/en not_active Ceased
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