JP3979996B2 - 固体支持体を使用してrnaを精製するための組成物および方法 - Google Patents

固体支持体を使用してrnaを精製するための組成物および方法 Download PDF

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Publication number
JP3979996B2
JP3979996B2 JP2003536461A JP2003536461A JP3979996B2 JP 3979996 B2 JP3979996 B2 JP 3979996B2 JP 2003536461 A JP2003536461 A JP 2003536461A JP 2003536461 A JP2003536461 A JP 2003536461A JP 3979996 B2 JP3979996 B2 JP 3979996B2
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Prior art keywords
rna
solid support
solution
biological material
salt
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Japanese (ja)
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JP2005505305A (ja
JP2005505305A5 (enExample
Inventor
エレン エム. ヒース,
ジョン エム. ジュニア ウェイジズ,
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Gentra Systems Inc
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Gentra Systems Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2003536461A 2001-10-12 2001-10-12 固体支持体を使用してrnaを精製するための組成物および方法 Expired - Lifetime JP3979996B2 (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2001/032073 WO2003033739A1 (en) 2001-10-12 2001-10-12 Compositions and methods for using a solid support to purify rna

Publications (3)

Publication Number Publication Date
JP2005505305A JP2005505305A (ja) 2005-02-24
JP2005505305A5 JP2005505305A5 (enExample) 2006-01-05
JP3979996B2 true JP3979996B2 (ja) 2007-09-19

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ID=21742909

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JP2003536461A Expired - Lifetime JP3979996B2 (ja) 2001-10-12 2001-10-12 固体支持体を使用してrnaを精製するための組成物および方法

Country Status (5)

Country Link
EP (1) EP1438426A1 (enExample)
JP (1) JP3979996B2 (enExample)
AU (1) AU2002211719B2 (enExample)
CA (1) CA2463317A1 (enExample)
WO (1) WO2003033739A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523463A (ja) * 2003-04-16 2006-10-19 ジェントラ システムズ インコーポレイテッド 固体支持体を使用してrnaを精製するための、組成物および方法

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516731A (ja) 1997-09-17 2001-10-02 ジエントラ・システムズ・インコーポレーテツド 核酸を単離するための装置および方法
US20050032105A1 (en) 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
DK1442045T3 (da) * 2001-11-06 2009-02-09 Cortex Biochem Inc Isolering og oprensning af nukleinsyrer
AU2002359522A1 (en) 2001-11-28 2003-06-10 Applera Corporation Compositions and methods of selective nucleic acid isolation
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
JP3983125B2 (ja) * 2002-07-19 2007-09-26 富士フイルム株式会社 核酸の分離精製方法
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
JP4435787B2 (ja) 2003-12-16 2010-03-24 キアジェン ノース アメリカン ホールディングス,インコーポレイティド タンパク質を変性させるための処方物および方法
WO2005093065A1 (en) * 2004-03-16 2005-10-06 Roche Diagnostics Gmbh Improved method of isolating nucleic acids
EP1809744B1 (en) * 2004-11-05 2018-06-06 QIAGEN North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
JP4956727B2 (ja) * 2005-08-30 2012-06-20 倉敷紡績株式会社 Rna分離精製方法
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
MX347611B (es) 2011-06-19 2017-05-04 Abogen Inc Dispositivos, soluciones y metodos para recoleccion de muestras.
ES2981185T3 (es) * 2013-03-14 2024-10-07 Translate Bio Inc Métodos para la purificación de ARN mensajero
RU2717644C2 (ru) 2014-03-07 2020-03-24 ДиЭнЭй ГЕНОТЕК ИНК. Способ, композиция и набор для стабилизации дезоксирибонуклеиновых кислот в биологических образцах
MX373952B (es) 2014-04-25 2020-07-13 Shire Human Genetic Therapies Métodos de purificación de arn mensajero.
WO2020041793A1 (en) 2018-08-24 2020-02-27 Translate Bio, Inc. Methods for purification of messenger rna

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
AU6230594A (en) * 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
ATE184013T1 (de) * 1994-06-14 1999-09-15 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523463A (ja) * 2003-04-16 2006-10-19 ジェントラ システムズ インコーポレイテッド 固体支持体を使用してrnaを精製するための、組成物および方法

Also Published As

Publication number Publication date
AU2002211719B2 (en) 2007-06-14
CA2463317A1 (en) 2003-04-24
JP2005505305A (ja) 2005-02-24
EP1438426A1 (en) 2004-07-21
WO2003033739A1 (en) 2003-04-24

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